LOCUS pPR220.08 6544 bp ds-DNA circular 28-DEC-2015 DEFINITION . COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers terminator complement(2687..2792) /label="T0" /ApEinfo_revcolor=#ff0517 /ApEinfo_fwdcolor=#ff0517 RBS 793..806 /label="RBS pprotet" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 700..792 /label="ho1 (RBS 80.077)" /ApEinfo_revcolor=#ac0c14 /ApEinfo_fwdcolor=#ac0c14 misc_feature 3942..4010 /label="TM-2" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 3674..3761 /label="apFAB655 " /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 52..63 /label="B0034" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 312..334 /label="Sequencing primer fw SR129" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e rep_origin complement(2907..3594) /label="p15A" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 1703..1722 /label="Sequencing primer fw SR272" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 795..806 /label="B0034" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 6006..6024 /label="PR188" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 418..436 /label="Sequencing primer rev SR358" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 70..680 /label="ho1 (RBS 80.077)" /ApEinfo_revcolor=#ac0c14 /ApEinfo_fwdcolor=#ac0c14 misc_feature 3574..3594 /label="Sequencing Primer fw SR26" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 6306..6434 /label="Terminator B0015" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 misc_feature 1..35 /label="Constitutive promoter J23108" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 4418..4436 /label="PR186" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 3759..6293 /label="uirS" /ApEinfo_revcolor=#8000ff /ApEinfo_fwdcolor=#8000ff misc_feature 1888..1893 /label="Sequencencing primer rev SR27" /ApEinfo_revcolor=#8000ff /ApEinfo_fwdcolor=#8000ff CDS 681..699 /label="ho1 (RBS 80.077)" /ApEinfo_revcolor=#ac0c14 /ApEinfo_fwdcolor=#ac0c14 misc_feature 1888..2679 /label="SpecR" /ApEinfo_revcolor=#ff00ff /ApEinfo_fwdcolor=#ff00ff misc_feature 1938..1954 /label="Cloning primer fw SR256" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 6458..6461 /label="New Feature" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 1919..1941 /label="Cloning primer rev SR255" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 857..857 /label="Silent mutation to C" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 3846..3914 /label="TM-1" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 6435..6461 /label="Cloning primer rev SR386" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter 1740..1887 /label="specR_promoter_from_pKD13" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS 813..1559 /label="pcyA" /ApEinfo_revcolor=#ac0c14 /ApEinfo_fwdcolor=#ac0c14 misc_feature 1566..1694 /label="Terminator B0015" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 misc_feature 5201..5221 /label="PR187" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 4029..4097 /label="TM-3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1938..1941 /label="New Feature" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 696..699 /label="oh" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 RBS 50..63 /label="RBS pprotet" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1874..1887 /label="Sequencing primer rev SR27" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 3635..3669 /label="Bba_J23111" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd ORIGIN 1 CTGACAGCTA GCTCAGTCCT AGGTATAATG CTAGCTCACA CAGAATTCAT TAAAGAGGAG 61 AAAGGTACCA TGAGTGTCAA CTTAGCTTCC CAGTTGCGGG AAGGGACGAA AAAATCCCAC 121 TCCATGGCGG AGAACGTCGG CTTTGTCAAA TGCTTCCTCA AGGGCGTTGT CGAGAAAAAT 181 TCCTACCGTA AGCTGGTTGG CAATCTCTAC TTTGTCTACA GTGCCATGGA AGAGGAAATG 241 GCAAAATTTA AGGACCATCC CATCCTCAGC CACATTTACT TCCCCGAACT CAACCGCAAA 301 CAAAGCCTAG AGCAAGACCT GCAATTCTAT TACGGCTCCA ACTGGCGGCA AGAAGTGAAA 361 ATTTCTGCCG CTGGCCAAGC CTATGTGGAC CGAGTCCGGC AAGTGGCCGC TACGGCCCCT 421 GAATTGTTGG TGGCCCATTC CTACACCCGT TACCTGGGGG ATCTTTCCGG CGGTCAAATT 481 CTCAAGAAAA TTGCCCAAAA TGCCATGAAT CTCCACGATG GTGGCACAGC TTTCTATGAA 541 TTTGCCGACA TTGATGACGA AAAGGCTTTT AAAAATACCT ACCGTCAAGC TATGAATGAT 601 CTGCCCATTG ACCAAGCCAC CGCCGAACGG ATTGTGGATG AAGCCAATGA CGCCTTTGCC 661 ATGAACATGA AAATGTTCAA CGAACTTGAA GGCAACCTGA TCAAGGCGAT CGGCATTATG 721 GTGTTCAACA GCCTCACCCG TCGCCGCAGT CAAGGCAGCA CCGAAGTTGG CCTCGCCACC 781 TCCGAAGGCT AGTTAAAGAG GAGAAAGGAT CCATGGCCGT CACTGATTTA AGTTTGACCA 841 ATTCTTCCCT GATGCCTACG TTGAACCCGA TGATTCAACA GTTGGCCCTG GCGATCGCCG 901 CTAGTTGGCA AAGTTTACCC CTCAAGCCCT ATCAATTGCC GGAGGATTTG GGCTACGTAG 961 AAGGCCGCCT GGAAGGGGAA AAGTTAGTGA TTGAAAATCG GTGCTACCAA ACGCCCCAGT 1021 TTCGCAAAAT GCATTTGGAG TTGGCCAAGG TGGGCAAAGG GTTGGATATT CTCCACTGTG 1081 TAATGTTTCC TGAGCCTTTA TACGGTCTAC CTTTGTTTGG CTGTGACATT GTGGCCGGCC 1141 CCGGTGGAGT AAGTGCGGCT ATTGCGGATC TATCCCCCAC CCAAAGCGAT CGCCAATTGC 1201 CCGCAGCGTA CCAAAAATCA TTGGCAGAGC TAGGCCAGCC AGAATTTGAG CAACAACGGG 1261 AATTGCCCCC CTGGGGAGAA ATATTTTCTG AATATTGTTT ATTCATCCGT CCCAGCAATG 1321 TCACTGAAGA AGAAAGATTT GTACAAAGGG TAGTGGACTT TTTGCAAATT CATTGTCACC 1381 AATCCATCGT TGCCGAACCC TTGTCTGAAG CTCAAACTTT GGAGCACCGT CAGGGGCAAA 1441 TTCATTACTG CCAACAACAA CAGAAAAATG ATAAAACCCG TCGGGTACTG GAAAAAGCTT 1501 TTGGGGAAGC TTGGGCGGAA CGGTATATGA GCCAAGTCTT ATTTGATGTT ATCCAATAAT 1561 CTAGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT 1621 CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC 1681 TTTCTGCGTT TATAGGTACC CGGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTA 1741 GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA 1801 GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT 1861 CAAGAGACAG GATGAGGATC GTTTCGCATG AGGGAAGCGG TGATCGCCGA AGTATCGACT 1921 CAACTATCAG AGGTAGTTGG CGTCATCGAG CGCCATCTCG AACCGACGTT GCTGGCCGTA 1981 CATTTGTACG GCTCCGCAGT GGATGGCGGC CTGAAGCCAC ACAGTGATAT TGATTTGCTG 2041 GTTACGGTGA CCGTAAGGCT TGATGAAACA ACGCGGCGAG CTTTGATCAA CGACCTTTTG 2101 GAAACTTCGG CTTCCCCTGG AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT CACCATTGTT 2161 GTGCACGACG ACATCATTCC GTGGCGTTAT CCAGCTAAGC GCGAACTGCA ATTTGGAGAA 2221 TGGCAGCGCA ATGACATTCT TGCAGGTATC TTCGAGCCAG CCACGATCGA CATTGATCTG 2281 GCTATCTTGC TGACAAAAGC AAGAGAACAT AGCGTTGCCT TGGTAGGTCC AGCGGCGGAG 2341 GAACTCTTTG ATCCGGTTCC TGAACAGGAT CTATTTGAGG CGCTAAATGA AACCTTAACG 2401 CTATGGAACT CGCCGCCCGA CTGGGCTGGC GATGAGCGAA ATGTAGTGCT TACGTTGTCC 2461 CGCATTTGGT ACAGCGCAGT AACCGGCAAA ATCGCGCCGA AGGATGTCGC TGCCGACTGG 2521 GCAATGGAGC GCCTGCCGGC CCAGTATCAG CCCGTCATAC TTGAAGCTAG ACAGGCTTAT 2581 CTTGGACAAG AAGAAGATCG CTTGGCCTCG CGCGCAGATC AGTTGGAAGA ATTTGTCCAC 2641 TACGTGAAAG GCGAGATCAC CAAGGTAGTC GGCAAATAAG AGCTCGCTTG GACTCCTGTT 2701 GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC TCGGTTGCCG 2761 CCGGGCGTTT TTTATTGGTG AGAATCCAAG CACTAGTAAC AACTTATATC GTATGGGGCT 2821 GACTTCAGGT GCTACATTTG AAGAGATAAA TTGCACTGAA ATCTAGTAAT ATTTTATCTG 2881 ATTAATAAGA TGATCTTCTT GAGATCGTTT TGGTCTGCGC GTAATCTCTT GCTCTGAAAA 2941 CGAAAAAACC GCCTTGCAGG GCGGTTTTTC GAAGGTTCTC TGAGCTACCA ACTCTTTGAA 3001 CCGAGGTAAC TGGCTTGGAG GAGCGCAGTC ACCAAAACTT GTCCTTTCAG TTTAGCCTTA 3061 ACCGGCGCAT GACTTCAAGA CTAACTCCTC TAAATCAATT ACCAGTGGCT GCTGCCAGTG 3121 GTGCTTTTGC ATGTCTTTCC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3181 GGTCGGACTG AACGGGGGGT TCGTGCATAC AGTCCAGCTT GGAGCGAACT GCCTACCCGG 3241 AACTGAGTGT CAGGCGTGGA ATGAGACAAA CGCGGCCATA ACAGCGGAAT GACACCGGTA 3301 AACCGAAAGG CAGGAACAGG AGAGCGCACG AGGGAGCCGC CAGGGGGAAA CGCCTGGTAT 3361 CTTTATAGTC CTGTCGGGTT TCGCCACCAC TGATTTGAGC GTCAGATTTC GTGATGCTTG 3421 TCAGGGGGGC GGAGCCTATG GAAAAACGGC TTTGCCGCGG CCCTCTCACT TCCCTGTTAA 3481 GTATCTTCCT GGCATCTTCC AGGAAATCTC CGCCCCGTTC GTAAGCCATT TCCGCTCGCC 3541 GCAGTCGAAC GACCGAGCGT AGCGAGTCAG TGAGCGAGGA AGCGGAATAT ATCCCGAAGC 3601 GGCATGCATT TACGTTGACA CCATCGAATG CTGAttgacg gctagctcag tcctaggtat 3661 agtgctagcC AGTGGGCCCA AGTTCACTTA AAAAGGAGAT CAACAATGAA AGCAATTTTC 3721 GTACTGAAAC ATCTTAATCA TGCTTTGGAG TTTTTCTGAT GGCAATCACC GCATTTACCC 3781 TGGGGGATTT TTTCCAAGCC AACAGCTATA TACCCCACGG CCATTGCTAC CTTTGGCAAA 3841 CTCCCCTGGT CTGGCTCCAC GTCAGCGCTG ATTTTTTCAC GGCGATCGCC TACTACTCTA 3901 TTCCCTTAAC TCTGCTCTAC TTTTTGCGGA AGCGCCAAGA TATTCCTTTT CCTAATATTA 3961 TTTTCCTGTT TAGTACTTTT ATTTTATGCT GTGGCACCAG TCACTTTTTT GACATTATCA 4021 CCCTTTGGTA CCCTATTTAT TGGATATCCG GTACAGTCAA AGCCTCCATG GCGATCGTCT 4081 CTATTATCAC TGTGTTTGAA TTAATTCAAA TTGTACCCAA TGCCCTAAAT TTAAAATCCC 4141 CCACGGAATT AGCTACCCTC AATCTGGCTC TAAATCAAGA AATTAAAGAA AGGCAAACAG 4201 CGGAAATAGC ATTGCAGGAG TTAAACAATA ATCTTGAAAA AAGAGTGGAA GACCGCACTA 4261 CCCAATTAGC AAAAATAAAT CAACAATTAG AACAGGAAAT TGAAGATAAA ACCCGGGCAA 4321 AAGAAGATCT AGAAAAAAAT AAAGATCAAT TGGCGCAATT GGCTGCTATT GTCGAATCTT 4381 CCCAGGACGC AATTATTAGT AAAACCCTAG ATGGTAACAT CACCAGTTGG AACGAGTCTG 4441 CGGAAAGATT ATTTGGCTAC ACAGCAGAAG AAATGATTGG AAGTCATATC ACCAAACTTA 4501 TTCCTGAGGA ATTAATCTTG GAAGAAGACC TTATTGCTGA ATGCATCCGC CAAGGTCAGA 4561 GAATTAACAC CTACGAAACT CAAAGACAGC GTAAAGATGG TACTAAAATT GATGTTGCCC 4621 TGACCATTTC TCCCATTCGA GATGAACACA AAAATGTAGT GGGAGCCTCC AAAATTGTTA 4681 GGGATATTAC CGCCAGATTG GATGTGGAAA ATGCCCTACG GGAAAGTCAA TATTTTATTG 4741 AAAAATTAGC TAATTATTCT CCCCAGATTC TTTATATTTT AGACCCCATT GCTTGGAAAA 4801 ATATTTATGT CAATTATCAA TCCTTCGAAA TCCTAGGCTA CACTCCAGAA GAATTTAAAA 4861 ATGGGGGAAC GGAGCTGTTG TTAAATATTG TGCATCCCGA TGACATCCCA ACCCTTTACG 4921 AAAATAAGAA TTTTTGGCAA AAGTCCCAGG AAGGCCAAGT GTTGACCACT GAATATCGAA 4981 TGCGCCACAA AAATGGTTCT TGGCGGTGGC TACGTTCTCG GGAAGTGGTT TTTGCTAGGG 5041 ATGATTATGG GCAAGTTACT AAGGTTTTGG GCACAGCCCA GGATATTAGT GATAGCAAAG 5101 AACAGGAACA AAGACTTTAT GAGCAAGGCC GCCGCGAATC ATTATTAAGG GAAATTACCC 5161 AGCGTATCCG TCAGTCTTTA GATCTGCCAA CTATTTTTAA CACAGTAGTG CAGGAAATTC 5221 GTCAGTTTTT GGAAGCAGAT CGGGTAGTTA TTTTTCAGTT TTCACCCGAC TCTGACTTTT 5281 CCGTTGGTAA TATTGTGGCA GAGTCGGTAT TGGCTCCATT TAAGCCAATC ATTAATAGTG 5341 CAATTGAAGA AACTTGTTTT AGTAATAACT ATGCCCAAAG GTATCAGCAG GGCAGAATTC 5401 AGGTCATTGA GGATATTCAC CAGTCCCATC TTAGGCAATG CCACATTGAC TTTCTTGCCA 5461 GGCTACAGGT CAGGGCAAAC CTAGTGCTAC CACTAATTAA TGATGCCATT TTGTGGGGCT 5521 TATTGTGTAT TCATCAATGT GACAGTTCTA GAGTTTGGGA ACAAACAGAA ATTGATCTGC 5581 TCAAGCAGAT CACTAATCAG TTTGAAATTG CCATCCAACA GGCTACTTTG TACGAGCAGG 5641 CTCAACAAGA ATTGGCTTCT AAAAATCAGC TTTTTGTACA ATTAACCAAC GAATTAGAGC 5701 AAAAAAAAGT ATTGCTTAAG GAAATTCATC ATCGAGTTAA AAACAATTTG CAAATTATGT 5761 CTAGTTTACT TTATTTGCAA TTTAGTAAGG CTTCTCCAGC CATCCAGCAA TTGAGTGAGG 5821 AATACCAGAA TCGCATTCAA TCCATGGCAT TGATTCATGA GCAGTTATAT CGTAGTGAAG 5881 ACTTAGCTAA TATTGATTTT AGTCAATATC TTAAAAATTT GACTCACAAT ATCTGCCAAA 5941 GTTACGGCTG TAATACTGAT AGTATTAAAA TTAAATTGTT GGTTGAACAA GTTAAAGTTC 6001 CTTTAGAGCA GTCCATTCCT TTGGGATTAA TTATTCAGGA ATTAGTTTCC AATGCTTTAA 6061 AACATGCTTT CCCAACGACG GAAGGGGAAA TTTCGATTAA ATTCACTTCA ATGAACAGTC 6121 ACTATAGTTT ACAGGTGTGG GATAACGGAG TTGGAATTTC CAGGGATATA GATTTGGAAA 6181 ATACAGATAG TTTGGGCATG CAACTTATCT ACAGCCTAAC TGAACAACTC CAGGGAGAAT 6241 TGCACTATGA ATATGTCGGT GGCGCTCAAT TTGGGTTGGA ATTTTCACTT TAGTAATAAT 6301 CTAGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT 6361 CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC 6421 TTTCTGCGTT TATAGTAAAT CACTGCATAA TTCGTGTCGA TTAAACCGCC TAAGTCCCCC 6481 AGGAAAGGGG GATATAACAG TATAGATTTT GTCAGCCTTC AGCTTGGCTT TACCGTCAAA 6541 AAAA //