LOCUS       BC-A1-008               4090 bp    DNA     circular UNA 13-NOV-2016
DEFINITION  Low luxR Promoter, Low luxR Sensitivity, Low GFP RBS, Low GFP
            Degradation Rate.
ACCESSION   urn.local...5m-6u33cf3
VERSION     urn.local...5m-6u33cf3
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(241..855)
                     /gene="rep (pMB1)"
                     /label="rep (pMB1) misc"
     misc_feature    1125..1130
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI"
                     /note="Geneious type: restriction site"
     CDS             complement(1140..1799)
                     /gene="Chloramphenicol resistance"
                     /label="Chloramphenicol resistance CDS"
     misc_feature    2030..2035
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     promoter        2036..2070
                     /created_by="User"
                     /label="BBa_J23109"
     misc_feature    2071..2076
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     RBS             2077..2088
                     /created_by="User"
                     /label="BBa_B0034"
     misc_feature    2089..2094
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     cds             2095..2847
                     /X-Transferred_From="BC-A1-001"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BC-A1-001, BC-A1-003"
                     /Transferred_Similarity="100.00%"
                     /label="luxR"
     misc_feature    2851..2858
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    2852..2857
                     /Recognition_pattern="C^GGCCG"
                     /annotation_group="CGGCCG"
                     /label="EagI"
                     /note="Geneious type: restriction site"
     terminator      2892..3020
                     /created_by="User"
                     /label="Double Terminator"
     misc_feature    3021..3026
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    3105..3110
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     promoter        3111..3165
                     /created_by="User"
                     /label="pLux"
     misc_feature    3166..3171
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     RBS             3172..3182
                     /created_by="User"
                     /label="BBa_B0033"
     misc_feature    3186..3191
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     cds             3189..3905
                     /created_by="User"
                     /X-Transferred_From="BC-A1-002; BC-A1-004"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BC-A1-002, BC-A1-004, BC-A1-006"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     misc_feature    3909..3914
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     terminator      3948..4076
                     /created_by="User"
                     /label="Double Terminator"
     misc_feature    4077..4082
                     /Recognition_pattern="C^CCGGG"
                     /annotation_group="CCCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    4077..4082
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc tgcagtttac
     2041 agctagctca gtcctaggga ctgtgctagc ggatccaaag aggagaaaga attcatgaaa
     2101 aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcgtg tagaagcaat
     2161 aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta
     2221 ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac
     2281 cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta
     2341 gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta
     2401 aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt
     2461 agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa
     2521 aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt
     2581 ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat
     2641 ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat
     2701 atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa
     2761 atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca
     2821 attgattgcc catactttaa aaattaataa gcggccgcca ctgatagtgc tagtgtagat
     2881 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2941 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     3001 tgggcctttc tgcgtttata ggtacctact agagaggttc tgttaagtaa ctgaacccaa
     3061 tgtcgttagt gacgcttacc tcttaagagg tcactgacct aacaaagctt acctgtagga
     3121 tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaaactag ttcacacagg
     3181 actagcatat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     3241 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     3301 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     3361 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcaca
     3421 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     3481 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     3541 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     3601 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     3661 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     3721 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     3781 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     3841 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     3901 aataataatc tagacactga tagtgctagt gtagatcact actagagcca ggcatcaaat
     3961 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     4021 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatacccg
     4081 ggtactagag
//
