LOCUS       BC-A1-010               4091 bp    DNA     circular UNA 13-NOV-2016
DEFINITION  High luxR Promoter, High luxR Sensitivity, High GFP RBS, Low GFP
            Degradation Rate.
ACCESSION   urn.local...5o-6u33cf3
VERSION     urn.local...5o-6u33cf3
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(241..855)
                     /gene="rep (pMB1)"
                     /label="rep (pMB1) misc"
     misc_feature    1125..1130
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI"
                     /note="Geneious type: restriction site"
     CDS             complement(1140..1799)
                     /gene="Chloramphenicol resistance"
                     /label="Chloramphenicol resistance CDS"
     misc_feature    2030..2035
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     promoter        2036..2070
                     /created_by="User"
                     /label="BBa_J23100"
     misc_feature    2071..2076
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     RBS             2077..2088
                     /created_by="User"
                     /label="BBa_B0034"
     misc_feature    2089..2094
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     cds             2095..2847
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="BC-A1-009"
                     /Transferred_Similarity="100.00%"
                     /label="luxR-G2F"
     misc_feature    2851..2858
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    2852..2857
                     /Recognition_pattern="C^GGCCG"
                     /annotation_group="CGGCCG"
                     /label="EagI"
                     /note="Geneious type: restriction site"
     terminator      2892..3020
                     /created_by="User"
                     /label="Double Terminator"
     misc_feature    3021..3026
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    3105..3110
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     promoter        3111..3165
                     /created_by="User"
                     /label="pLux"
     misc_feature    3166..3171
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     RBS             3172..3183
                     /created_by="User"
                     /label="BBa_B0034"
     misc_feature    3187..3192
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     cds             3190..3906
                     /created_by="User"
                     /X-Transferred_From="BC-A1-002; BC-A1-004; BC-A1-006"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="BC-A1-002, BC-A1-004, BC-A1-006,
                     BC-A1-008"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     misc_feature    3910..3915
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     terminator      3949..4077
                     /created_by="User"
                     /label="Double Terminator"
     misc_feature    4078..4083
                     /Recognition_pattern="C^CCGGG"
                     /annotation_group="CCCGGG"
                     /label="XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    4078..4083
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc tgcagttgac
     2041 ggctagctca gtcctaggta cagtgctagc ggatccaaag aggagaaaga attcatgaaa
     2101 aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcgtg tagaagcaat
     2161 aatgatatta atcaatgctt atctgatatg gctaaaatgg tacattgtga atattattta
     2221 ctcgcgttca tttatcctca ttctatggtt aaatcagata tttcaatcct agataattac
     2281 cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta
     2341 gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta
     2401 aataaaaaat ctccaaatgt aattaaagaa gcgaaaacag caggtcttat cactgggttt
     2461 agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa
     2521 aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt
     2581 ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat
     2641 ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat
     2701 atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa
     2761 atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca
     2821 attgattgcc catactttaa aaattaataa gcggccgcca ctgatagtgc tagtgtagat
     2881 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2941 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     3001 tgggcctttc tgcgtttata ggtacctact agagaggttc tgttaagtaa ctgaacccaa
     3061 tgtcgttagt gacgcttacc tcttaagagg tcactgacct aacaaagctt acctgtagga
     3121 tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaaactag taaagaggag
     3181 aaatagcata tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     3241 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     3301 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     3361 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcac
     3421 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     3481 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     3541 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     3601 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     3661 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     3721 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     3781 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     3841 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     3901 aaataataat ctagacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa
     3961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     4021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttataccc
     4081 gggtactaga g
//
