LOCUS       SQT1665                 8653 bp    DNA     circular SYN 18-MAY-2016
DEFINITION  pCAG-CFP.
ACCESSION   urn.local...d-64y2bmt
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="pCAG-CFP"
                     /mol_type="other DNA"
     misc_feature    84..371
                     /label="CAG_enhancer"
     CDS             255..734
                     /translation="MPSTPPIDVNDGKWPAWHYAQYMTLWDFPTWQYIYVLVIAITMG
                     RGEPHVLLHSPHLPPLPTPNFVFIYFLIILCSDGGGGGGGARQAGRGGARGGAGRGGE
                     VRRQPIRAARSESFLLWRGGGGGGPIKSEARGGRESLRCLRPVPRSAPPRAARPGSD*
                     "
                     /label="ORF frame 3"
     CDS             complement(802..1533)
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSA
                     RFGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPP
                     PPARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLC
                     SPRSPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSR
                     SPPHKGPSRSPLRLSRSHRKETSRH*"
                     /label="ORF frame 2"
     CDS             complement(843..1346)
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT
                     KGPPGAL*"
                     /label="ORF frame 3"
     misc_feature    1678..1697
                     /translation="MFMPSSFSYSSWATCWLLCCLIILAKNSAVDGTAGPGSTGRHHG
                     EQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLRQADPEVHLHHRQAARALAH
                     PRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRPGAHHLLQGRRQLQDPRRGEVRGR
                     HPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLYHRRQAEERHQGQLQDPPQHRGRQ
                     RAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQRPQREARSHGPAGVRDRRRDHSRH
                     GRAVQVKRPHSSGAGCLSEGGGWCGQCPGSQIPLRSFSLCQKLWGHHEAP*"
                     /label="pCAG_F_primer"
     misc_feature    1756..1761
                     /Recognition_pattern="A^CCGGT"
                     /annotation_group="ACCGGT"
                     /label="AgeI"
                     /note="Geneious type: restriction site"
     source          <1769..>5590
                     /organism="pY016 (pcDNA3.1-hLbCpf1)"
                     /mol_type="other DNA"
     CDS             1769..5452
                     /created_by="User"
                     /modified_by="User"
                     /label="LbCpf1"
     primer_bind     2278..2297
                     /Sequence="CATCGCCTTCAGGTGTATCA"
                     /Hairpin_Tm="45.6"
                     /Tm="57.4"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="seq F1"
     primer_bind     2883..2901
                     /Sequence="AGAAGAAGGCCGTGGTGAC"
                     /Hairpin_Tm="None"
                     /Tm="59.6"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /created_by="primer3"
                     /label="seq F2"
     primer_bind     3487..3506
                     /Sequence="TGTGAACGGCAATTACGAGA"
                     /Hairpin_Tm="34.9"
                     /Tm="57.6"
                     /Self_Dimer_Tm="8.5"
                     /%GC="45.0"
                     /created_by="primer3"
                     /label="seq F3"
     primer_bind     4086..4105
                     /Sequence="CCAAGAAAACCACAACCCTG"
                     /Hairpin_Tm="None"
                     /Tm="57.1"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="seq F4"
     primer_bind     4680..4700
                     /Sequence="TGAAGGGCTATCAGATCACCA"
                     /Hairpin_Tm="None"
                     /Tm="58.2"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="seq F5"
     primer_bind     5283..5300
                     /Sequence="ATGCCATCCTGCCAAAGA"
                     /Hairpin_Tm="None"
                     /Tm="56.5"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="seq F6"
     ORF             5453..5506
                     /created_by="User"
                     /label="nucleoplasmin NLS"
     ORF             5507..5587
                     /created_by="User"
                     /label="3x HA tag"
     misc_feature    5592..5599
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     terminator      5609..6110
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="rb_glob_PA_terminator"
     promoter        complement(6152..6170)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="M13_reverse_primer"
     misc_feature    complement(6169..6191)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="M13_pUC_rev_primer"
     promoter        complement(6205..6234)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="lac_promoter"
     promoter        6340..6519
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="SV40_promoter"
     rep_origin      6375..6452
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="SV40_origin"
     misc_feature    6437..6456
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="SV40pro_F_primer"
     terminator      6533..6664
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="SV40_PA_terminator"
     misc_feature    6621..6640
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="EBV_rev_primer"
     rep_origin      complement(6888..7507)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="pBR322_origin"
     gene            complement(7662..8522)
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /label="Ampicillin"
     CDS             complement(7662..8522)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 3"
     misc_feature    complement(7802..7807)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     promoter        complement(8564..8592)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gagcaagctg gagaagttta caaactgcta
     1801 ctccctgtct aagaccctga ggttcaaggc catccctgtg ggcaagaccc aggagaacat
     1861 cgacaataag cggctgctgg tggaggacga gaagagagcc gaggattata agggcgtgaa
     1921 gaagctgctg gatcgctact atctgtcttt tatcaacgac gtgctgcaca gcatcaagct
     1981 gaagaatctg aacaattaca tcagcctgtt ccggaagaaa accagaaccg agaaggagaa
     2041 taaggagctg gagaacctgg agatcaatct gcggaaggag atcgccaagg ccttcaaggg
     2101 caacgagggc tacaagtccc tgtttaagaa ggatatcatc gagacaatcc tgccagagtt
     2161 cctggacgat aaggacgaga tcgccctggt gaacagcttc aatggcttta ccacagcctt
     2221 caccggcttc tttgataaca gagagaatat gttttccgag gaggccaaga gcacatccat
     2281 cgccttcagg tgtatcaacg agaatctgac ccgctacatc tctaatatgg acatcttcga
     2341 gaaggtggac gccatctttg ataagcacga ggtgcaggag atcaaggaga agatcctgaa
     2401 cagcgactat gatgtggagg atttctttga gggcgagttc tttaactttg tgctgacaca
     2461 ggagggcatc gacgtgtata acgccatcat cggcggcttc gtgaccgaga gcggcgagaa
     2521 gatcaagggc ctgaacgagt acatcaacct gtataatcag aaaaccaagc agaagctgcc
     2581 taagtttaag ccactgtata agcaggtgct gagcgatcgg gagtctctga gcttctacgg
     2641 cgagggctat acatccgatg aggaggtgct ggaggtgttt agaaacaccc tgaacaagaa
     2701 cagcgagatc ttcagctcca tcaagaagct ggagaagctg ttcaagaatt ttgacgagta
     2761 ctctagcgcc ggcatctttg tgaagaacgg ccccgccatc agcacaatct ccaaggatat
     2821 cttcggcgag tggaacgtga tccgggacaa gtggaatgcc gagtatgacg atatccacct
     2881 gaagaagaag gccgtggtga ccgagaagta cgaggacgat cggagaaagt ccttcaagaa
     2941 gatcggctcc ttttctctgg agcagctgca ggagtacgcc gacgccgatc tgtctgtggt
     3001 ggagaagctg aaggagatca tcatccagaa ggtggatgag atctacaagg tgtatggctc
     3061 ctctgagaag ctgttcgacg ccgattttgt gctggagaag agcctgaaga agaacgacgc
     3121 cgtggtggcc atcatgaagg acctgctgga ttctgtgaag agcttcgaga attacatcaa
     3181 ggccttcttt ggcgagggca aggagacaaa cagggacgag tccttctatg gcgattttgt
     3241 gctggcctac gacatcctgc tgaaggtgga ccacatctac gatgccatcc gcaattatgt
     3301 gacccagaag ccctactcta aggataagtt caagctgtat tttcagaacc ctcagttcat
     3361 gggcggctgg gacaaggata aggagacaga ctatcgggcc accatcctga gatacggctc
     3421 caagtactat ctggccatca tggataagaa gtacgccaag tgcctgcaga agatcgacaa
     3481 ggacgatgtg aacggcaatt acgagaagat caactataag ctgctgcccg gccctaataa
     3541 gatgctgcca aaggtgttct tttctaagaa gtggatggcc tactataacc ccagcgagga
     3601 catccagaag atctacaaga atggcacatt caagaagggc gatatgttta acctgaatga
     3661 ctgtcacaag ctgatcgact tctttaagga tagcatctcc cggtatccaa agtggtccaa
     3721 tgcctacgat ttcaactttt ctgagacaga gaagtataag gacatcgccg gcttttacag
     3781 agaggtggag gagcagggct ataaggtgag cttcgagtct gccagcaaga aggaggtgga
     3841 taagctggtg gaggagggca agctgtatat gttccagatc tataacaagg acttttccga
     3901 taagtctcac ggcacaccca atctgcacac catgtacttc aagctgctgt ttgacgagaa
     3961 caatcacgga cagatcaggc tgagcggagg agcagagctg ttcatgaggc gcgcctccct
     4021 gaagaaggag gagctggtgg tgcacccagc caactcccct atcgccaaca agaatccaga
     4081 taatcccaag aaaaccacaa ccctgtccta cgacgtgtat aaggataaga ggttttctga
     4141 ggaccagtac gagctgcaca tcccaatcgc catcaataag tgccccaaga acatcttcaa
     4201 gatcaataca gaggtgcgcg tgctgctgaa gcacgacgat aacccctatg tgatcggcat
     4261 cgataggggc gagcgcaatc tgctgtatat cgtggtggtg gacggcaagg gcaacatcgt
     4321 ggagcagtat tccctgaacg agatcatcaa caacttcaac ggcatcagga tcaagacaga
     4381 ttaccactct ctgctggaca agaaggagaa ggagaggttc gaggcccgcc agaactggac
     4441 ctccatcgag aatatcaagg agctgaaggc cggctatatc tctcaggtgg tgcacaagat
     4501 ctgcgagctg gtggagaagt acgatgccgt gatcgccctg gaggacctga actctggctt
     4561 taagaatagc cgcgtgaagg tggagaagca ggtgtatcag aagttcgaga agatgctgat
     4621 cgataagctg aactacatgg tggacaagaa gtctaatcct tgtgcaacag gcggcgccct
     4681 gaagggctat cagatcacca ataagttcga gagctttaag tccatgtcta cccagaacgg
     4741 cttcatcttt tacatccctg cctggctgac atccaagatc gatccatcta ccggctttgt
     4801 gaacctgctg aaaaccaagt ataccagcat cgccgattcc aagaagttca tcagctcctt
     4861 tgacaggatc atgtacgtgc ccgaggagga tctgttcgag tttgccctgg actataagaa
     4921 cttctctcgc acagacgccg attacatcaa gaagtggaag ctgtactcct acggcaaccg
     4981 gatcagaatc ttccggaatc ctaagaagaa caacgtgttc gactgggagg aggtgtgcct
     5041 gaccagcgcc tataaggagc tgttcaacaa gtacggcatc aattatcagc agggcgatat
     5101 cagagccctg ctgtgcgagc agtccgacaa ggccttctac tctagcttta tggccctgat
     5161 gagcctgatg ctgcagatgc ggaacagcat cacaggccgc accgacgtgg attttctgat
     5221 cagccctgtg aagaactccg acggcatctt ctacgatagc cggaactatg aggcccagga
     5281 gaatgccatc ctgccaaaga acgccgacgc caatggcgcc tataacatcg ccagaaaggt
     5341 gctgtgggcc atcggccagt tcaagaaggc cgaggacgag aagctggata aggtgaagat
     5401 cgccatctct aacaaggagt ggctggagta cgcccagacc agcgtgaagc acaaaaggcc
     5461 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
     5521 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
     5581 ctatgcctaa agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     5641 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     5701 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     5761 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5821 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg
     5881 ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg
     5941 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     6001 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     6061 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat
     6121 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6301 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     6361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6481 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     6541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6661 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7081 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7321 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8641 aaagtgccac ctg
//
