LOCUS       Exported                9140 bp ds-DNA     circular SYN 06-JUN-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSB700 Cerulean-Weissman scaffold (2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 6, 2016 from SnapGene 3.1.2
            http://www.snapgene.com
COMMENT     LOCUS pLVX-ZsGreen1-N1 8763 bp DNA circular 7-OCT-2009 COMMENT 
            Created by Clontech Laboratories Inc. http://www.clontech.com 
            COMMENT Cat. No. 632565
FEATURES             Location/Qualifiers
     source          1..9140
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..635
                     /label=5'\LTR
                     /note="5' LTR"
                     /note="5'\LTR"
     misc_binding    636..653
                     /label=PBS
                     /note="PBS"
     misc_feature    685..822
                     /label=packaging\signal
                     /note="packaging signal"
                     /note="packaging\signal"
     misc_binding    1303..1536
                     /label=RRE
                     /note="RRE"
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
                     /note="cPPT/CTS (DNA flap)"
                     /note="cPPT/CTS\(DNA\flap)"
     promoter        2246..2495
                     /label=Human U6 Promoter
                     /note="Human U6 Promoter"
     misc_feature    2465..2490
                     /label=User
                     /note="User"
     misc_feature    complement(2496..2501)
                     /label=CGTCTC(1/5)
                     /note="CGTCTC(1/5)"
                     /note="CGTCTC(1/5);Geneious type: restriction site"
     misc_feature    2497..2515
                     /label=GAGCAGGTATTAATGACCTGCCTA
                     /note="GAGCAGGTATTAATGACCTGCCTA"
                     /note="GAGCAGGTATTAATGACCTGCCTA;Geneious type: Editing 
                     History Replacement"
     misc_feature    2509..2514
                     /label=CGTCTC(1/5)(1)
                     /note="CGTCTC(1/5)(1)"
                     /note="CGTCTC(1/5);Geneious type: restriction site"
     misc_feature    2516..2671
                     /label=weissman sgRNA tail ms2
                     /note="weissman sgRNA tail ms2"
     misc_feature    2678..2683
                     /label=G^AATTC
                     /note="G^AATTC"
                     /note="G^AATTC;Geneious type: restriction site"
     misc_feature    complement(2679..2703)
                     /label=User(1)
                     /note="User(1)"
                     /note="User"
     enhancer        2748..3035
                     /label=CAG enhancer H CMV IE enhancer like
                     /note="CAG enhancer H CMV IE enhancer like ""CAG enhanc"
                     /note="""CAG enhancer"" H CMV IE enhancer like;""CAG 
                     enhancer"" H CMV IE enhancer like pCAGGS"
     promoter        3054..4294
                     /label=CAG promoter like pCAGGS
                     /note="CAG promoter like pCAGGS"
     misc_feature    complement(4349..4369)
                     /label=CAGGS cloning primer
                     /note="CAGGS cloning primer"
     misc_feature    4350..4369
                     /label=pCAG_F_primer from Addgene
                     /note="pCAG_F_primer from Addgene"
     misc_feature    4370..4375
                     /label=Kozak
                     /note="Kozak"
     misc_feature    4376..5095
                     /label=CFP
                     /note="CFP"
     misc_feature    5108..5699
                     /label=WPRE
                     /note="WPRE"
     LTR             5902..6538
                     /label=3'\LTR
                     /note="3' LTR"
                     /note="3'\LTR"
     misc_feature    6714..6743
                     /label=lac promoter
                     /note="lac promoter"
     rep_origin      complement(7008..7681)
                     /direction=LEFT
                     /label=pUC\ori
                     /note="pUC ori"
                     /note="pUC\ori"
     CDS             complement(7826..8822)
                     /label=Amp(R) with promoter 7449-8379
                     /note="Amp(R) with promoter 7449-8379"
     misc_feature    8842..8973
                     /label=SV40 polyA
                     /note="SV40 polyA"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cattagtgaa cggatctcga cggtatcgat cacgagacta
     2221 gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt catatttgca
     2281 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
     2341 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
     2401 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
     2461 ggctttatat atcttgtgga aaggacgaaa caccggagac gattaatgcg tctcggttta
     2521 agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa
     2581 aaagtggcac cgagtcggtg cgggagcaca tgaggatcac ccatgtgcga ctcccacagt
     2641 cactggggag tcttcccttt ttttgttttt tatgtctgaa ttctcgacct cgagacaaat
     2701 ggcagtattc gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2761 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2821 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2881 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2941 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3001 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
     3061 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
     3121 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggcgc gcgccaggcg
     3181 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     3241 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     3301 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg
     3361 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     3421 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     3481 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     3541 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     3601 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     3661 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     3721 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     3781 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     3841 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     3901 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     3961 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     4021 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     4081 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     4141 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     4201 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     4261 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     4321 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgg ccaccatggt
     4381 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4441 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4501 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4561 gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     4621 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4681 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     4741 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4801 ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     4861 caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     4921 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4981 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5041 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgt
     5101 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5161 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5221 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5281 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5341 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5401 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5461 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5521 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5581 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5641 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     5701 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     5761 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     5821 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5881 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc
     5941 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     6001 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     6061 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     6121 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     6181 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     6241 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     6301 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     6361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6541 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6601 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat
     6661 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6721 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6781 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6841 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8821 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8881 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8941 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     9001 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     9061 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     9121 tgtactacaa acttagtagt
//
