LOCUS       Exported                9177 bp ds-DNA     circular SYN 06-JUN-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4 (2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 6, 2016 from SnapGene 3.1.2
            http://www.snapgene.com
COMMENT     LOCUS pLVX-ZsGreen1-N1 8763 bp DNA circular 7-OCT-2009 COMMENT 
            Created by Clontech Laboratories Inc. http://www.clontech.com 
            COMMENT Cat. No. 632565
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..635
                     /label=5'\LTR
                     /note="5' LTR"
                     /note="5'\LTR"
     misc_binding    636..653
                     /label=PBS
                     /note="PBS"
     misc_feature    685..822
                     /label=packaging\signal
                     /note="packaging signal"
                     /note="packaging\signal"
     misc_binding    1303..1536
                     /label=RRE
                     /note="RRE"
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
                     /note="cPPT/CTS (DNA flap)"
                     /note="cPPT/CTS\(DNA\flap)"
     promoter        2246..2495
                     /label=Human U6 Promoter
                     /note="Human U6 Promoter"
     misc_feature    2465..2490
                     /label=User
                     /note="User"
     misc_feature    complement(2496..2501)
                     /label=CGTCTC(1/5)
                     /note="CGTCTC(1/5)"
                     /note="CGTCTC(1/5);Geneious type: restriction site"
     misc_feature    2497..2515
                     /label=GAGCAGGTATTAATGACCTGCCTA
                     /note="GAGCAGGTATTAATGACCTGCCTA"
                     /note="GAGCAGGTATTAATGACCTGCCTA;Geneious type: Editing 
                     History Replacement"
     misc_feature    2509..2514
                     /label=CGTCTC(1/5)(1)
                     /note="CGTCTC(1/5)(1)"
                     /note="CGTCTC(1/5);Geneious type: restriction site"
     misc_feature    2516..2651
                     /label=zhang 2.0 guide
                     /note="zhang 2.0 guide"
     misc_feature    2652..2684
                     /label=weissman sgRNA tail ms2
                     /note="weissman sgRNA tail ms2"
     misc_feature    2652..2684
                     /label=Weissman part
                     /note="Weissman part"
     misc_feature    2686
                     /label=weissman sgRNA tail ms2(1)
                     /note="weissman sgRNA tail ms2(1)"
     misc_feature    2686
                     /label=Weissman part(1)
                     /note="Weissman part(1)"
     misc_feature    2688..2690
                     /label=weissman sgRNA tail ms2(2)
                     /note="weissman sgRNA tail ms2(2)"
     misc_feature    2688..2690
                     /label=Weissman part(2)
                     /note="Weissman part(2)"
     misc_feature    2692..2707
                     /label=weissman sgRNA tail ms2(3)
                     /note="weissman sgRNA tail ms2(3)"
     misc_feature    2692..2707
                     /label=Weissman part(3)
                     /note="Weissman part(3)"
     misc_feature    2708..2712
                     /label=zhang 2.0 guide(1)
                     /note="zhang 2.0 guide(1)"
     misc_RNA        2713..2714
                     /label=User(1)
                     /note="User(1)"
                     /note="User;sgRNA backbone"
     misc_feature    2715..2720
                     /label=G^AATTC
                     /note="G^AATTC"
                     /note="G^AATTC;Geneious type: restriction site"
     misc_feature    complement(2716..2740)
                     /label=User(2)
                     /note="User(2)"
                     /note="User"
     enhancer        2785..3072
                     /label=CAG enhancer H CMV IE enhancer like
                     /note="CAG enhancer H CMV IE enhancer like ""CAG enhanc"
                     /note="""CAG enhancer"" H CMV IE enhancer like;""CAG 
                     enhancer"" H CMV IE enhancer like pCAGGS"
     promoter        3091..4331
                     /label=CAG promoter like pCAGGS
                     /note="CAG promoter like pCAGGS"
     misc_feature    complement(4386..4406)
                     /label=CAGGS cloning primer
                     /note="CAGGS cloning primer"
     misc_feature    4387..4406
                     /label=pCAG_F_primer from Addgene
                     /note="pCAG_F_primer from Addgene"
     misc_feature    4407..4412
                     /label=Kozak
                     /note="Kozak"
     misc_feature    4413..5132
                     /label=CFP
                     /note="CFP"
     misc_feature    5145..5736
                     /label=WPRE
                     /note="WPRE"
     LTR             5939..6575
                     /label=3'\LTR
                     /note="3' LTR"
                     /note="3'\LTR"
     misc_feature    6751..6780
                     /label=lac promoter
                     /note="lac promoter"
     rep_origin      complement(7045..7718)
                     /direction=LEFT
                     /label=pUC\ori
                     /note="pUC ori"
                     /note="pUC\ori"
     CDS             complement(7863..8859)
                     /label=Amp(R) with promoter 7449-8379
                     /note="Amp(R) with promoter 7449-8379"
     misc_feature    8879..9010
                     /label=SV40 polyA
                     /note="SV40 polyA"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cattagtgaa cggatctcga cggtatcgat cacgagacta
     2221 gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt catatttgca
     2281 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
     2341 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
     2401 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
     2461 ggctttatat atcttgtgga aaggacgaaa caccggagac gattaatgcg tctcggtttt
     2521 agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc
     2581 tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac
     2641 cgagtcggtg cgggagcaca tgaggatcac ccatgtgcga ctccgagagt aactggggag
     2701 tcttcccttt ttttgaattc tcgacctcga gacaaatggc agtattcgtc attagttcat
     2761 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2821 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2881 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2941 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3001 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3061 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     3121 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     3181 gcgatggggg cggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
     3241 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     3301 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     3361 gggagtcgct gcgacgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     3421 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     3481 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     3541 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     3601 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     3661 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     3721 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     3781 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     3841 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     3901 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     3961 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     4021 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     4081 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     4141 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     4201 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     4261 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     4321 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     4381 tcctgggcaa cgtgctggtt attgtggcca ccatggtgag caagggcgag gagctgttca
     4441 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4501 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4561 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
     4621 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4681 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4741 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4801 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgcc atcagcgaca
     4861 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     4921 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4981 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     5041 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5101 tcactctcgg catggacgag ctgtacaagt aaagcgtctg gaacaatcaa cctctggatt
     5161 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5221 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5281 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5341 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5401 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5461 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5521 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5581 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5641 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5701 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     5761 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     5821 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     5881 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
     5941 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     6001 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     6061 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     6121 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     6181 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     6241 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     6301 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     6361 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     6421 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6481 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6541 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6601 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
     6661 gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6721 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8821 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     8881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9001 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     9061 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     9121 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
