LOCUS       PCDNA6.2PLITRBLA        7800 bp    DNA     circular SYN 20-JAN-2014
DEFINITION  Ligation of Fragment 2 into Fragment 2
ACCESSION   PCDNA6.2PLITRBLA
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  Fragment 2
               Fragment ends: BglII
               Fragment size: 4922
            Insert molecule:  Fragment 2
               Fragment ends: BglII
               Fragment size: 2878
FEATURES             Location/Qualifiers
     CDS             210..229
                     /gene="T7 Pro"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             258..382
                     /gene="attR1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             811..1116
                     /gene="ccdB"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1439..2119
                     /gene="Chloramphenicol"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(2400..2524)
                     /gene="attR2"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2618..2889
                     /gene="TK PA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2891..2901
                     /gene="'TetR"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2907..3038
                     /gene="SV40 PA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             3458..3886
                     /gene="f1 Ori"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             3896..4326
                     /gene="SV40 Promoter"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             4276..4342
                     /gene="EM7 Pro"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             4346..4741
                     /gene="Blasticidin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             4899..5029
                     /gene="SV40 PA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     polyA_signal    complement(5423..5453)
                     /label=SV40 polyA
                     /ApEinfo_fwdcolor=#ffc6b8
                     /ApEinfo_revcolor=#ffc6b8
                     /SECDrawAs="Info only"
                     /SECName="SV40 polyA'"
     misc_feature    5470..5697
                     /label=ITR2 SB
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ITR2 SB"
     CDS             5718..6388
                     /gene="pUC Ori"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             complement(6533..7393)
                     /gene="Ampicillin"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    7554..7780
                     /label=ITR SB
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ITR SB"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattctctg gctaactaga
      181 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      241 ggctagttaa gctatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
      301 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
      361 cacaacatat ccagtcacta tgaatcaact acttagatgg tattagtgac ctgtagtcga
      421 ccgacagcct tccaaatgtt cttcgggtga tgctgccaac ttagtcgacc gacagccttc
      481 caaatgttct tctcaaacgg aatcgtcgta tccagcctac tcgctattgt cctcaatgcc
      541 gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt gtgtgacaaa
      601 ataaaaacat ctacctattc atatacgcta gtgtcatagt cctgaaaatc atctgcatca
      661 agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc atctggattt
      721 tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt atcgacctgc
      781 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
      841 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
      901 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
      961 cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac
     1021 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
     1081 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag
     1141 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
     1201 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac
     1261 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
     1321 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
     1381 tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt
     1441 aagccggatc cacgcggcgt ttacgccccc cctgccactc atcgcagtac tgttgtaatt
     1501 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1561 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1621 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1681 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1741 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1801 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1861 tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca
     1921 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1981 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     2041 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     2101 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     2161 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     2221 caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag
     2281 gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt
     2341 cgggtgatgc tgccaactta gtcgactaca ggtcactaat accatctaag tagttgattc
     2401 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     2461 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     2521 tggttgatct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
     2581 tcgattctac gcgtaccggt tagtaatgag tttaaacggg ggaggctaac tgaaacacgg
     2641 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     2701 cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     2761 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     2821 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     2881 tgccatagca gatcttatta aagcagaact tgtttattgc agcttataat ggttacaaat
     2941 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3001 gtttgtccaa actcatcaat gtatcttatc atgtctggtc gaatccatca cactggcggc
     3061 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     3121 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     3181 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3421 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     3481 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3601 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3661 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3721 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3781 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3841 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     3901 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3961 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4021 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4081 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4141 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4201 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4261 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     4321 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
     4381 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     4441 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     4501 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     4561 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     4621 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     4681 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     4741 agcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     4801 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     4861 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     4921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     5041 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5401 caggaaagaa catgtgctct tcgttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5461 gaatactaat tgagtgtatg taaacttctg acccactggg aatgtgatga aagaaataaa
     5521 agctgaaatg aatcattctc tctactatta ttctgatatt tcacattctt aaaataaagt
     5581 ggtgatccta actgacctaa gacagggaat ttttactagg attaaatgtc aggaattgtg
     5641 aaaaagtgag tttaaatgta tttggctaag gtgtatgtaa acttccgact tcaactgtat
     5701 agagtactgc tagccacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5761 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5821 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5881 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5941 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6001 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6061 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6121 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6181 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6241 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6301 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7501 catttccccg aaaagtgcca cctgacgtcg acgtgcgcat cgaactccct atacagttga
     7561 agtcggaagt ttacatacac ttaagttgga gtcattaaaa ctcgtttttc aactactcca
     7621 caaatttctt gttaacaaac aatagttttg gcaagtcagt taggacatct actttgtgca
     7681 tgacacaagt catttttcca acaattgttt acagacagat tatttcactt ataattcact
     7741 gtatcacaat tccagtgggt cagaagttta catacactaa gttgactgtg ttacggcgtc
//
