LOCUS pDGE011_#632_M4E 4375 bp DNA circular SYN 11-FEB-1999 DEFINITION Cloning vector pUC57, complete sequence. ACCESSION Y14837 VERSION Y14837.1 GI:2440162 KEYWORDS beta-galactosidase; beta-lactamase; bla gene; lac repressor; lacI gene; lacZ gene. SOURCE Cloning vector pUC57 (unknown) ORGANISM Cloning vector pUC57 other sequences; artificial sequences; vectors. REFERENCE 1 AUTHORS Markausakas,A. and Dreguniene,G. TITLE A new cloning vector pUC57 JOURNAL Unpublished REFERENCE 2 (bases 1 to 2710) AUTHORS Markauskas,A. TITLE Direct Submission JOURNAL Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, Vilnius 2028, LITHUANIA FEATURES Location/Qualifiers primer 359..375 /label=M13f misc_feature complement(446..529) /feature_type="sgRNA backbone" /label=sgBB CDS complement(567..872) /label=ccdB CDS complement(1214..1873) /label=CmR misc_feature complement(1993) /feature_type="Transcription Start Site" /label=TSS promoter complement(1994..2082) /label=pU6-26 TATA_signal complement(2017..2024) /label=TATA primer complement(2154..2170) /label=M13r protein_bind complement(2176..2196) /note="lac repressor" /bound_moiety="lacI" misc_feature 2541..3155 /note="replicon of pMB1" rep_origin complement(2555) CDS complement(3315..4175) /gene="bla" /function="confers resistance to ampicilin" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="CAA75111.1" /db_xref="GI:4379389" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" mat_peptide complement(3318..4106) /gene="bla" /product="beta-lactamase" sig_peptide complement(4107..4175) /gene="bla" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCTCGCGAA 421 TGCATCTAGA Taaaggtctc tAAGCAAAAA AAAGCACCGA CTCGGTGCCA CTTTTTCAAG 481 TTGATAACGG ACTAGCCTTA TTTTAACTTG CTATTTCTAG CTCTAAAACA AgtcttcGAC 541 TGGCTGTGTA TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT AATGGCGTTT 601 TTGATGTCAT TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC GGAaACCGGC 661 ACACTGGCCA TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG CACCACCGGG 721 TAAAGTTCAC GGGAGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG GATCACCATC 781 CGTCGCCCGG GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG ATAACGGCTC 841 TCTCTTTTAT AGGTGTAAAC CTTAAACTGC ATTTCACCAG cCCCTGTTCT CGTCAGCAAA 901 AGAGCCGTTC ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT TTTCCGCTTT 961 CCAGCGTTCG GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA CCAGACCGGA 1021 GATATTGACA TCATATATGC CTTGAGCAAC TGATAGCTGT CGCTGTCAAC TGTCACTGTA 1081 ATACGCTGCT TCATAGCAtA CCTCTTTTTG ACATACTTCG GGTATACATA TCAGTATATA 1141 TTCTTATACC GCAAAAATCA GCGCGCAAAT ACGCATACTG TTATCTGGCT TTTAGTAAGC 1201 CGGATCCACG CGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA 1261 GCATTCTGCC GACATGGAAG CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA 1321 TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT 1381 TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA 1441 CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG 1501 CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA 1561 GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC 1621 ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAATTC CGGATGAGCA TTCATCAGGC 1681 GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA 1741 AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA 1801 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA 1861 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC 1921 CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT 1981 CTCAGAAGAC ttCAATCACT ACTTCGACTC TAGCTGTATA TAAACTCAGC TTCGTTTTCT 2041 TATCTAAGCG ATGTGGGACT TTTGAAGATT GTTTTCAACT TACCATtgag accaaaATCG 2101 GATCCCGGGC CCGTCGACTG CAGAGGCCTG CATGCAAGCT TGGCGTAATC ATGGTCATAG 2161 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 2221 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 2281 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 2341 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 2401 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 2461 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 2521 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 2581 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 2641 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 2701 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 2761 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 2821 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 2881 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 2941 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 3001 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 3061 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 3121 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 3181 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 3241 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 3301 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 3361 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 3421 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAaC CACGCTCACC GGCTCCAGAT 3481 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 3541 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 3601 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 3661 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 3721 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 3781 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 3841 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 3901 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 3961 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 4021 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 4081 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 4141 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 4201 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 4261 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC 4321 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTC //