LOCUS       pDGE011_#632_M4E      4375 bp    DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     Y14837.1  GI:2440162
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; lacI
            gene; lacZ gene.
SOURCE      Cloning vector pUC57 (unknown)
  ORGANISM  Cloning vector pUC57
            other sequences; artificial sequences; vectors.
REFERENCE   1  
  AUTHORS   Markausakas,A. and Dreguniene,G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Markauskas,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8,
            Vilnius 2028, LITHUANIA
FEATURES             Location/Qualifiers
     primer          359..375
                     /label=M13f
     misc_feature    complement(446..529)
                     /feature_type="sgRNA backbone"
                     /label=sgBB
     CDS             complement(567..872)
                     /label=ccdB
     CDS             complement(1214..1873)
                     /label=CmR
     misc_feature    complement(1993)
                     /feature_type="Transcription Start Site"
                     /label=TSS
     promoter        complement(1994..2082)
                     /label=pU6-26
     TATA_signal     complement(2017..2024)
                     /label=TATA
     primer          complement(2154..2170)
                     /label=M13r
     protein_bind    complement(2176..2196)
                     /note="lac repressor"
                     /bound_moiety="lacI"
     misc_feature    2541..3155
                     /note="replicon of pMB1"
     rep_origin      complement(2555)
     CDS             complement(3315..4175)
                     /gene="bla"
                     /function="confers resistance to ampicilin"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="CAA75111.1"
                     /db_xref="GI:4379389"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     mat_peptide     complement(3318..4106)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4107..4175)
                     /gene="bla"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCTCGCGAA
      421 TGCATCTAGA Taaaggtctc tAAGCAAAAA AAAGCACCGA CTCGGTGCCA CTTTTTCAAG
      481 TTGATAACGG ACTAGCCTTA TTTTAACTTG CTATTTCTAG CTCTAAAACA AgtcttcGAC
      541 TGGCTGTGTA TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT AATGGCGTTT
      601 TTGATGTCAT TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC GGAaACCGGC
      661 ACACTGGCCA TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG CACCACCGGG
      721 TAAAGTTCAC GGGAGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG GATCACCATC
      781 CGTCGCCCGG GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG ATAACGGCTC
      841 TCTCTTTTAT AGGTGTAAAC CTTAAACTGC ATTTCACCAG cCCCTGTTCT CGTCAGCAAA
      901 AGAGCCGTTC ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT TTTCCGCTTT
      961 CCAGCGTTCG GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA CCAGACCGGA
     1021 GATATTGACA TCATATATGC CTTGAGCAAC TGATAGCTGT CGCTGTCAAC TGTCACTGTA
     1081 ATACGCTGCT TCATAGCAtA CCTCTTTTTG ACATACTTCG GGTATACATA TCAGTATATA
     1141 TTCTTATACC GCAAAAATCA GCGCGCAAAT ACGCATACTG TTATCTGGCT TTTAGTAAGC
     1201 CGGATCCACG CGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA
     1261 GCATTCTGCC GACATGGAAG CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA
     1321 TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT
     1381 TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA
     1441 CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG
     1501 CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA
     1561 GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC
     1621 ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAATTC CGGATGAGCA TTCATCAGGC
     1681 GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA
     1741 AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA
     1801 ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA
     1861 TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC
     1921 CCGGTAGTGA TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT
     1981 CTCAGAAGAC ttCAATCACT ACTTCGACTC TAGCTGTATA TAAACTCAGC TTCGTTTTCT
     2041 TATCTAAGCG ATGTGGGACT TTTGAAGATT GTTTTCAACT TACCATtgag accaaaATCG
     2101 GATCCCGGGC CCGTCGACTG CAGAGGCCTG CATGCAAGCT TGGCGTAATC ATGGTCATAG
     2161 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
     2221 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
     2281 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
     2341 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
     2401 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
     2461 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     2521 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     2581 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     2641 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     2701 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     2761 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     2821 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     2881 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     2941 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
     3001 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     3061 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     3121 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     3181 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     3241 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     3301 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     3361 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     3421 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAaC CACGCTCACC GGCTCCAGAT
     3481 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     3541 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     3601 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     3661 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     3721 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     3781 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     3841 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     3901 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     3961 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     4021 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     4081 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     4141 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     4201 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     4261 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC
     4321 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTC
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