LOCUS       pDGE006_#616_M1E      4375 bp    DNA     circular SYN 11-FEB-1999
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     Y14837.1  GI:2440162
KEYWORDS    beta-galactosidase; beta-lactamase; bla gene; lac repressor; lacI
            gene; lacZ gene.
SOURCE      Cloning vector pUC57 (unknown)
  ORGANISM  Cloning vector pUC57
            other sequences; artificial sequences; vectors.
REFERENCE   1  
  AUTHORS   Markausakas,A. and Dreguniene,G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Markauskas,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8,
            Vilnius 2028, LITHUANIA
FEATURES             Location/Qualifiers
     primer          359..375
                     /label=M13f
     misc_feature    complement(445..528)
                     /feature_type="sgRNA backbone"
                     /label=sgBB
     CDS             complement(566..871)
                     /label=ccdB
     CDS             complement(1217..1876)
                     /label=CmR
     misc_feature    complement(1996)
                     /feature_type="Transcription Start Site"
                     /label=TSS
     promoter        complement(1997..2085)
                     /label=pU6-26
     TATA_signal     complement(2020..2027)
                     /label=TATA
     primer          complement(2154..2170)
                     /label=M13r
     protein_bind    complement(2176..2196)
                     /note="lac repressor"
                     /bound_moiety="lacI"
     protein_bind    complement(2243..2280)
                     /bound_moiety="CAP"
     misc_feature    2541..3155
                     /note="replicon of pMB1"
     rep_origin      complement(2555)
     CDS             complement(3315..4175)
                     /gene="bla"
                     /function="confers resistance to ampicilin"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="CAA75111.1"
                     /db_xref="GI:4379389"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(3315..4175)
                     /gene="bla"
     mat_peptide     complement(3318..4106)
                     /gene="bla"
                     /product="beta-lactamase"
     sig_peptide     complement(4107..4175)
                     /gene="bla"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCTCGCGAA
      421 TGCATCTAGA Taaggtctct AAGCAAAAAA AAGCACCGAC TCGGTGCCAC TTTTTCAAGT
      481 TGATAACGGA CTAGCCTTAT TTTAACTTGC TATTTCTAGC TCTAAAACAA gtcttcGACT
      541 GGCTGTGTAT AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT
      601 TGATGTCATT TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG GAaACCGGCA
      661 CACTGGCCAT ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT
      721 AAAGTTCACG GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC
      781 GTCGCCCGGG CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT
      841 CTCTTTTATA GGTGTAAACC TTAAACTGCA TTTCACCAGc CCCTGTTCTC GTCAGCAAAA
      901 GAGCCGTTCA TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT TTCCGCTTTC
      961 CAGCGTTCGG CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC CAGACCGGAG
     1021 ATATTGACAT CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT GTCACTGTAA
     1081 TACGCTGCTT CATAGCAtAC CTCTTTTTGA CATACTTCGG GTATACATAT CAGTATATAT
     1141 TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT TTAGTAAGCC
     1201 GGATCCACGC GAgcgtTTAC GCCCCcCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT
     1261 TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA ATCGCCAGCG
     1321 GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA
     1381 AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG
     1441 AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC
     1501 ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC
     1561 AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT
     1621 CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA TTCCGGATGA GCATTCATCA
     1681 GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT
     1741 TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT
     1801 GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG
     1861 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA
     1921 CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCGATCAA
     1981 CGTCTCAGAA GACttCAATC ACTACTTCGA CTCTAGCTGT ATATAAACTC AGCTTCGTTT
     2041 TCTTATCTAA GCGATGTGGG ACTTTTGAAG ATTGTTTTCA ACTTAGCAAt gagaccATCG
     2101 GATCCCGGGC CCGTCGACTG CAGAGGCCTG CATGCAAGCT TGGCGTAATC ATGGTCATAG
     2161 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
     2221 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
     2281 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
     2341 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
     2401 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
     2461 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     2521 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     2581 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     2641 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     2701 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     2761 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     2821 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     2881 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     2941 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
     3001 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     3061 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     3121 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     3181 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     3241 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     3301 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     3361 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     3421 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAaC CACGCTCACC GGCTCCAGAT
     3481 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     3541 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     3601 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     3661 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     3721 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     3781 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     3841 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     3901 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     3961 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     4021 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     4081 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     4141 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     4201 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     4261 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC
     4321 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTC
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