LOCUS pEMS2046\ssAAV\Ple257-emGFP\WPRE 7118 bp DNA circular 21-FEB-2013 SOURCE ORGANISM COMMENT http://www.informaxinc.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|653577105| COMMENT VNTDBDATE|654356999| COMMENT LSOWNER| COMMENT VNTNAME|pEMS2046 ssAAV Ple257-emGFP WPRE| COMMENT VNTAUTHORNAME|Jack Hickmott| COMMENT VNTOAUTHORNAME|User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0.932452 COMMENT #214#)) (CObjectList))) (CObjectList)) COMMENT #216=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1088 100) COMMENT (CObjectList) (CObjectList)) COMMENT #217=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1158 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers CDS 5071..5928 /vntifkey="4" /label=Amp-R misc_feature 6102..6690 /vntifkey="21" /label=Origin misc_feature 4133..4178 /vntifkey="21" /label=additional\AAV\sequences repeat_region 1..168 /vntifkey="34" /label=5'\ITR repeat_region complement(4141..4308) /vntifkey="34" /label=3'\ITR misc_feature 197..208 /vntifkey="21" /label=MCS\5'\end intron 2369..2541 /vntifkey="15" /label=Intron enhancer 3288..3874 /vntifkey="9" /label=WPRE exon 2542..3281 /vntifkey="61" /label=emGFP primer_bind 3365..3389 /vntifkey="28" /label=oEMS5939 primer_bind 2670..2690 /vntifkey="28" /label=oEMS5954 primer_bind 2933..2953 /vntifkey="28" /label=oEMS5955 promoter 215..2360 /vntifkey="29" /label=Ple257\Pax6\Enhancer BASE COUNT 1614 a 1905 c 1841 g 1758 t ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 aggaagatcg gaattcccta ggccggccac gcgtctccac tcctatccca tccccacgtc 241 cccgaaatga ataatgcagg caggagccaa gatttctgca ttagcgccac gttgccccgg 301 cggctggcgc taggatcccg aagtcggata atggaaatta aactgttgtt gtgtggcggg 361 gatgtcatta tgtgactgtt catattcaaa gtcaggcagc aatgaattgt aatcatttca 421 attatcttca atacactttc tttaggacac aagttgtcaa ggataaaaaa aaaaagtaca 481 ttcttgcggc tccgtgggtc cattttccag atggtttgtt actcttgctg cctgatttag 541 tttcggcttt tattccccag cctcagattc tcttgcagag actccgccgg agtgagcggc 601 ttctctggcg ctgcgggccg gctacgctgg tcggcaatcg gcctccgtgg gcctggcgtg 661 cgcattttgg ttgctttcag gtataattaa ctttgaacaa caaatatgcc tggagctctg 721 gatctagtgt ccccttgggg aagggggccg tcgtattata ctctgcgtcc ttcccagcct 781 ggggcggggt gggggctggc tgcagcagct gcacccactc cccaggccag cgctgcgcgt 841 cagaaaagca tgtgctgggc tgtacccact cctccgacag agcccggacc ctctccttcc 901 aacccagaga actcagggaa aagccgggat ccccggagcc tgccgtcctt tgttgaaatt 961 accctctgcc caatgttcgc tccctcctgt gcctgaacgt ggggctgcga caagttggat 1021 gtggtgtcct tgaagtatcc cttcgagcgc cctccgcccc cagactgggc aaaaagagta 1081 ggggtcgcct ttgaagccca agtccgcggc tcctatttgg actaatcccg atctttacac 1141 taatatcgcc tctcgggttt tcattatact catttgggag gaaatgagct tttgctgggc 1201 aaatagcacc ctgcacccca cttctcatag ctgtgcaggc ccggggcctg aacaaccccg 1261 caggcaatcc gggagctgcc gatattatat ttgtgaaatg tccttttgtt gaggctttta 1321 acaccttctt ataacgaatc atttgttctt ttctctttaa ttttgataga caattagcca 1381 tcagagatac accagttgac tgtaaccgag cattaaactc gcctttatgt aacgctaatt 1441 tagcatagct gctttgttta actttgctat ataagaacca ggttttgtag caaaaaaaaa 1501 aaggtgtaat tttatgtggg gcttcatttc ttttggccct gtacacagct agccctctcc 1561 tctttgtgaa gcgttctgct ggggcctgac gccatttccc gtgcctagca cctgcaggct 1621 ggggggagag agggaattag cattgatata gatttagttg aaatgtaatc tcttaaatct 1681 gtccaagatt cggggagata attacacttt cactgaattg ccaggggtca gcgtctctga 1741 ggacctgagg agttgattta ctaacaatag tgttacataa aaaatggcgt ttgagattat 1801 aatgttaatg gctgcggtcg ctggggaatg cgcccgcttc caactaattc catgtaactt 1861 gttttttttc ctcccttttc ctccctccct ttcttccagg ctccgtcttg gggatcggag 1921 gaggggacag ggtgattacc cagagaggta gctggccagc ctaagggcag agatcttggg 1981 gccctagtgc ccgaaggtgc ggaggagcgc actcggcaag actagtttcc tggggatcga 2041 ctctacgcca tacaggacgg cggcccaggc tggaccgggc cgggctagag cagtcacagg 2101 ccgggccaag gaaggccaaa gcaggggttg gagccggccg gaccctgggt ggggagaagc 2161 aggctcccgc ccggccggaa aactagtcgg cgcagagctg tgcccaactc tagccgccat 2221 gacgtcacgc gggccgggca gccaatgagg acggcgctgg cgtggatatt aaggaaagtt 2281 agcgcctgcc tgagcaccct cttttcttat cattgacatt taaactctgg ggcaggtcct 2341 cgcgtagaac gcggctgtca ggcgcgccga agttggtcgt gaggcactgg gcaggtaagt 2401 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga 2461 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc 2521 tccacaggtg tccactccca ggcggccgcc accatggtga gcaagggcga ggagctgttc 2581 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 2641 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 2701 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcac ctacggcgtg 2761 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 2821 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 2881 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 2941 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 3001 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc 3061 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 3121 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 3181 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 3241 atcactctcg gcatggacga gctgtacaag taagcggccg cgatcgctca acctctggat 3301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 3361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 3421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 3481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 3541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 3601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 3661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 3721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 3781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 3841 acgagtcgga tctccctttg ggccgcctcc ccgcgatcgc agacatgata agatacattg 3901 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 3961 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 4021 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt 4081 aaaacctcta caaatgtggt agtcgaaatt cccgataagg atcttcctag agcatggcta 4141 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt 4201 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg 4261 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc 4321 taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 4381 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 4441 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg 4501 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 4561 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 4621 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 4681 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 4741 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 4801 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 4861 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 4921 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt 4981 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 5041 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 5101 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 5161 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 5221 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 5281 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 5341 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 5401 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 5461 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 5521 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 5581 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 5641 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 5701 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 5761 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 5821 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 5881 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 5941 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 6001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 6061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 6121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 6181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 6241 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 6301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 6361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 6421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 6481 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 6541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 6601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 6661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 6721 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 6781 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 6841 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 6901 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 6961 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 7021 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 7081 aacagctatg accatgatta cgccagattt aattaagg //