LOCUS       pEMS2046\ssAAV\Ple257-emGFP\WPRE        7118 bp    DNA     circular     21-FEB-2013
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|653577105|
COMMENT     VNTDBDATE|654356999|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2046 ssAAV Ple257-emGFP WPRE|
COMMENT     VNTAUTHORNAME|Jack Hickmott|
COMMENT     VNTOAUTHORNAME|User|
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FEATURES             Location/Qualifiers
     CDS             5071..5928
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    6102..6690
                     /vntifkey="21"
                     /label=Origin
     misc_feature    4133..4178
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(4141..4308)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          2369..2541
                     /vntifkey="15"
                     /label=Intron
     enhancer        3288..3874
                     /vntifkey="9"
                     /label=WPRE
     exon            2542..3281
                     /vntifkey="61"
                     /label=emGFP
     primer_bind     3365..3389
                     /vntifkey="28"
                     /label=oEMS5939
     primer_bind     2670..2690
                     /vntifkey="28"
                     /label=oEMS5954
     primer_bind     2933..2953
                     /vntifkey="28"
                     /label=oEMS5955
     promoter        215..2360
                     /vntifkey="29"
                     /label=Ple257\Pax6\Enhancer
BASE COUNT     1614 a      1905 c      1841 g      1758 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgtctccac tcctatccca tccccacgtc 
      241 cccgaaatga ataatgcagg caggagccaa gatttctgca ttagcgccac gttgccccgg 
      301 cggctggcgc taggatcccg aagtcggata atggaaatta aactgttgtt gtgtggcggg 
      361 gatgtcatta tgtgactgtt catattcaaa gtcaggcagc aatgaattgt aatcatttca 
      421 attatcttca atacactttc tttaggacac aagttgtcaa ggataaaaaa aaaaagtaca 
      481 ttcttgcggc tccgtgggtc cattttccag atggtttgtt actcttgctg cctgatttag 
      541 tttcggcttt tattccccag cctcagattc tcttgcagag actccgccgg agtgagcggc 
      601 ttctctggcg ctgcgggccg gctacgctgg tcggcaatcg gcctccgtgg gcctggcgtg 
      661 cgcattttgg ttgctttcag gtataattaa ctttgaacaa caaatatgcc tggagctctg 
      721 gatctagtgt ccccttgggg aagggggccg tcgtattata ctctgcgtcc ttcccagcct 
      781 ggggcggggt gggggctggc tgcagcagct gcacccactc cccaggccag cgctgcgcgt 
      841 cagaaaagca tgtgctgggc tgtacccact cctccgacag agcccggacc ctctccttcc 
      901 aacccagaga actcagggaa aagccgggat ccccggagcc tgccgtcctt tgttgaaatt 
      961 accctctgcc caatgttcgc tccctcctgt gcctgaacgt ggggctgcga caagttggat 
     1021 gtggtgtcct tgaagtatcc cttcgagcgc cctccgcccc cagactgggc aaaaagagta 
     1081 ggggtcgcct ttgaagccca agtccgcggc tcctatttgg actaatcccg atctttacac 
     1141 taatatcgcc tctcgggttt tcattatact catttgggag gaaatgagct tttgctgggc 
     1201 aaatagcacc ctgcacccca cttctcatag ctgtgcaggc ccggggcctg aacaaccccg 
     1261 caggcaatcc gggagctgcc gatattatat ttgtgaaatg tccttttgtt gaggctttta 
     1321 acaccttctt ataacgaatc atttgttctt ttctctttaa ttttgataga caattagcca 
     1381 tcagagatac accagttgac tgtaaccgag cattaaactc gcctttatgt aacgctaatt 
     1441 tagcatagct gctttgttta actttgctat ataagaacca ggttttgtag caaaaaaaaa 
     1501 aaggtgtaat tttatgtggg gcttcatttc ttttggccct gtacacagct agccctctcc 
     1561 tctttgtgaa gcgttctgct ggggcctgac gccatttccc gtgcctagca cctgcaggct 
     1621 ggggggagag agggaattag cattgatata gatttagttg aaatgtaatc tcttaaatct 
     1681 gtccaagatt cggggagata attacacttt cactgaattg ccaggggtca gcgtctctga 
     1741 ggacctgagg agttgattta ctaacaatag tgttacataa aaaatggcgt ttgagattat 
     1801 aatgttaatg gctgcggtcg ctggggaatg cgcccgcttc caactaattc catgtaactt 
     1861 gttttttttc ctcccttttc ctccctccct ttcttccagg ctccgtcttg gggatcggag 
     1921 gaggggacag ggtgattacc cagagaggta gctggccagc ctaagggcag agatcttggg 
     1981 gccctagtgc ccgaaggtgc ggaggagcgc actcggcaag actagtttcc tggggatcga 
     2041 ctctacgcca tacaggacgg cggcccaggc tggaccgggc cgggctagag cagtcacagg 
     2101 ccgggccaag gaaggccaaa gcaggggttg gagccggccg gaccctgggt ggggagaagc 
     2161 aggctcccgc ccggccggaa aactagtcgg cgcagagctg tgcccaactc tagccgccat 
     2221 gacgtcacgc gggccgggca gccaatgagg acggcgctgg cgtggatatt aaggaaagtt 
     2281 agcgcctgcc tgagcaccct cttttcttat cattgacatt taaactctgg ggcaggtcct 
     2341 cgcgtagaac gcggctgtca ggcgcgccga agttggtcgt gaggcactgg gcaggtaagt 
     2401 atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga 
     2461 agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc 
     2521 tccacaggtg tccactccca ggcggccgcc accatggtga gcaagggcga ggagctgttc 
     2581 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 
     2641 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
     2701 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcac ctacggcgtg 
     2761 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 
     2821 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 
     2881 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 
     2941 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 
     3001 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc 
     3061 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 
     3121 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 
     3181 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 
     3241 atcactctcg gcatggacga gctgtacaag taagcggccg cgatcgctca acctctggat 
     3301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 
     3361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 
     3421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 
     3481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 
     3541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 
     3601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 
     3661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 
     3721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 
     3781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 
     3841 acgagtcgga tctccctttg ggccgcctcc ccgcgatcgc agacatgata agatacattg 
     3901 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 
     3961 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 
     4021 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt 
     4081 aaaacctcta caaatgtggt agtcgaaatt cccgataagg atcttcctag agcatggcta 
     4141 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt 
     4201 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg 
     4261 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc 
     4321 taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 
     4381 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 
     4441 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg 
     4501 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     4561 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     4621 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 
     4681 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     4741 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     4801 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 
     4861 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 
     4921 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     4981 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     5041 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 
     5101 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 
     5161 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 
     5221 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 
     5281 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 
     5341 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 
     5401 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 
     5461 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 
     5521 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 
     5581 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 
     5641 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 
     5701 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 
     5761 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 
     5821 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 
     5881 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 
     5941 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 
     6001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     6061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     6121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     6181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     6241 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     6301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     6361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     6421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     6481 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     6541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     6601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     6661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     6721 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 
     6781 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 
     6841 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 
     6901 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 
     6961 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 
     7021 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 
     7081 aacagctatg accatgatta cgccagattt aattaagg 
//
