LOCUS       pEMS2048\ssAAV\Ple259-emGFP\WPRE        7059 bp    DNA     circular     23-OCT-2015
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|745597984|
COMMENT     VNTDBDATE|748522224|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2048 ssAAV Ple259-emGFP WPRE|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|User|
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FEATURES             Location/Qualifiers
     CDS             5012..5869
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    6043..6631
                     /vntifkey="21"
                     /label=Origin
     misc_feature    4074..4119
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(4082..4249)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          2310..2482
                     /vntifkey="15"
                     /label=Intron
     enhancer        3229..3815
                     /vntifkey="9"
                     /label=WPRE
     exon            2483..3222
                     /vntifkey="61"
                     /label=emGFP
     primer_bind     3306..3330
                     /vntifkey="28"
                     /label=oEMS5939
     primer_bind     2611..2631
                     /vntifkey="28"
                     /label=oEMS5954
     primer_bind     2874..2894
                     /vntifkey="28"
                     /label=oEMS5955
     misc_feature    215..2301
                     /vntifkey="21"
                     /label=Ple259
     misc_feature    1848..2301
                     /vntifkey="21"
                     /label=P0
     misc_feature    1214..1847
                     /vntifkey="21"
                     /label=RR2
     misc_feature    215..1213
                     /vntifkey="21"
                     /label=RR1
     primer          complement(432..450)
                     /vntifkey="27"
                     /label=oEMS5983
BASE COUNT     1672 a      1815 c      1842 g      1730 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgtgggtgc cgggactgag agacaggaag 
      241 gaagcattgg agagccaacc tagataaggg taactagtaa ccctctgctg tggccagtat 
      301 ttacccaatg ctgagaaaaa gtcctcattt tcggtttatt tttatggatt taagctgtct 
      361 accaagtagt taaaaagtag accaggaatg gccttgtaga gtaaaatgtg cttcttgaaa 
      421 ttctttgctt gcaggcaggc agatcaataa tcaccatagg taaaacagaa aaaataatgc 
      481 atttaaaaaa aaacagagag aaatgatgct gtgactgttt tgttatttta cctctttttg 
      541 gggggcagac ctcttgaatg ggctctttat tgtaactctc tcttcccaat tgagctcccc 
      601 acggtgccgt tgtatctttt tacagcccct aacaactcac accagtaaca caattacctc 
      661 cagatattta caacaagaaa ttacttttct attttctcca ctcacccaaa agactttttt 
      721 tttttttcct ttgggaaagg tagggaggtg ttcgtacggg agcagcctcg gggacccctg 
      781 cactgggtca gggcttatga agctagaagc gtccctctgt tccctttgtg agttggtggg 
      841 ttgttggtac atttggttgg aagctgtgtt gctggttagg gagactcggt tttgctcctt 
      901 gggttcgagg aaagctggag aatagaagcc attgtttgcc gtctgtcggc tttgtcgacc 
      961 acgctcaccc cctcctgttc gtacttttta aagcagtgag gcgaggtaga cagggtgtgt 
     1021 cacagtacag ttaaaggggt gaagatctaa acgccaaaag agaagttaat cacaataagt 
     1081 gaggtttggg ataaaaagtt gggcttgccc ctttcaaagt cccagaaagc tgggaggtag 
     1141 atggagaggg ggccattggg aagttttttt ggtgtaggga gaggagtaga agataaaggg 
     1201 taagcagagt gttcctgcgt tctagacaga aaagcccctt ctgaccatgc attagtagta 
     1261 ctactccatt attcctgtta gaacaagtta aaagtaaggg ttgagcccaa aggccccagg 
     1321 aggggggtca tgtgcgcccc agtcactcag gctcccctcg cttctccggt tcgagttatg 
     1381 ccatcaagct aatatattgt gactgctctt ctctcctgtg acaaaggctt gcagctgcct 
     1441 ccaaatcaat agatgtcaaa gaaatatgaa aacaatcatg acacaaaact taaatcctgg 
     1501 ctggagccta cataatcaga aatgtgctac tgttcttcaa gccaccgatt aatttatccc 
     1561 aaactttagt caaattttta ttccggtacc tttttcccag cagatcctgc tacgtctgtc 
     1621 gggtttgtaa tgtaatttgt aattactgcc cttcatgtgg tccggtgcct tgaaccatct 
     1681 ttaattaaaa gcataattaa gggaagatct aaagaaagac aattaccaga tggtcttttt 
     1741 tttagaggcg gtagttgcgc agagaggggc tcggggtctg tccccgggac cccacgcctt 
     1801 gggagggggc ggcgggcccg aacggcgcct gcgcactgag gaggcctctt ggggatcgga 
     1861 ggaggggaca gggtgattac ccagagaggt agctggccag cctaagggca gagatcttgg 
     1921 ggccctagtg cccgaaggtg cggaggagcg cactcggcaa gactagtttc ctggggatcg 
     1981 actctacgcc atacaggacg gcggcccagg ctggaccggg ccgggctaga gcagtcacag 
     2041 gccgggccaa ggaaggccaa agcaggggtt ggagccggcc ggaccctggg tggggagaag 
     2101 caggctcccg cccggccgga aaactagtcg gcgcagagct gtgcccaact ctagccgcca 
     2161 tgacgtcacg cgggccgggc agccaatgag gacggcgctg gcgtggatat taaggaaagt 
     2221 tagcgcctgc ctgagcaccc tcttttctta tcattgacat ttaaactctg gggcaggtcc 
     2281 tcgcgtagaa cgcggctgtc aggcgcgccg aagttggtcg tgaggcactg ggcaggtaag 
     2341 tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag 
     2401 aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct 
     2461 ctccacaggt gtccactccc aggcggccgc caccatggtg agcaagggcg aggagctgtt 
     2521 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 
     2581 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 
     2641 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt 
     2701 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 
     2761 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 
     2821 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 
     2881 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 
     2941 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg 
     3001 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 
     3061 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 
     3121 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 
     3181 gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcgatcgctc aacctctgga 
     3241 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 
     3301 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 
     3361 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 
     3421 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 
     3481 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 
     3541 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 
     3601 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 
     3661 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 
     3721 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 
     3781 gacgagtcgg atctcccttt gggccgcctc cccgcgatcg cagacatgat aagatacatt 
     3841 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 
     3901 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 
     3961 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag 
     4021 taaaacctct acaaatgtgg tagtcgaaat tcccgataag gatcttccta gagcatggct 
     4081 acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt 
     4141 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 
     4201 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc cttaattaac 
     4261 ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 
     4321 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 
     4381 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 
     4441 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 
     4501 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 
     4561 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 
     4621 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 
     4681 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 
     4741 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 
     4801 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 
     4861 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     4921 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     4981 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
     5041 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
     5101 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
     5161 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
     5221 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
     5281 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
     5341 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
     5401 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
     5461 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
     5521 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
     5581 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
     5641 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
     5701 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
     5761 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
     5821 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
     5881 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     5941 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     6001 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     6061 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
     6121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
     6181 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
     6241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
     6301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
     6361 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
     6421 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
     6481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
     6541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
     6601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
     6661 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 
     6721 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 
     6781 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 
     6841 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
     6901 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 
     6961 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 
     7021 aaacagctat gaccatgatt acgccagatt taattaagg 
//
