LOCUS       pEMS2049\ssAAV\Ple260-emGFP\WPRE        7105 bp    DNA     circular      4-APR-2013
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|653576967|
COMMENT     VNTDBDATE|658410029|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2049 ssAAV Ple260-emGFP WPRE|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|User|
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FEATURES             Location/Qualifiers
     CDS             5058..5915
                     /vntifkey="4"
                     /label=Amp-R
     misc_feature    6089..6677
                     /vntifkey="21"
                     /label=Origin
     misc_feature    4120..4165
                     /vntifkey="21"
                     /label=additional\AAV\sequences
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
     repeat_region   complement(4128..4295)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..208
                     /vntifkey="21"
                     /label=MCS\5'\end
     intron          2356..2528
                     /vntifkey="15"
                     /label=Intron
     enhancer        3275..3861
                     /vntifkey="9"
                     /label=WPRE
     exon            2529..3268
                     /vntifkey="61"
                     /label=emGFP
     primer_bind     3352..3376
                     /vntifkey="28"
                     /label=oEMS5939
     primer_bind     2657..2677
                     /vntifkey="28"
                     /label=oEMS5954
     primer_bind     2920..2940
                     /vntifkey="28"
                     /label=oEMS5955
     promoter        215..2347
                     /vntifkey="29"
                     /label=Ple260\PAX6\retinal\enhancer
     primer          complement(404..423)
                     /vntifkey="27"
                     /label=oEMS5978
     primer          complement(500..519)
                     /vntifkey="27"
                     /label=oEMS5984
BASE COUNT     1596 a      1860 c      1933 g      1716 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcg gaattcccta ggccggccac gcgtcagact gggaattgcc gtggctatgc 
      241 ccgaagtttg gaaagtctct ctcggtctct ttctgggaca gcctttcttt ttccgggaag 
      301 cttcctgtgc ctagaggtgg ggctctgtag ctggaggggt agatggaact tgcgaagcaa 
      361 aggaaggcaa taaaaggaag ttaggagaaa ccggaaacgt gggccaggcc ttgctctgtt 
      421 ggagaagcgg tgtccccgca aagtccctgc ttgcgctggt cccggccgga gcccagatcc 
      481 cccaaacgcc tgtcacttct catcgagttg aggctccggc ccccacccgc gccttggtga 
      541 gggccccccc tcagccccaa gccttcggct accctgaaaa cgcggctccc ctcgaagggg 
      601 aaacaagtgc agttcaatct cgtctgagtg atctacaaat aaggacggaa agggtcgttt 
      661 tatcacggtc ccgtttgtcg tgacgatgca atttcccgga gcggagcact gtcacaaagt 
      721 gacaaggctg ccacaagcgc cccgactgat cttttcaatt agccttccat gcatgatccg 
      781 gagcgacttc cgcctatttc cagaaattaa gctcaaactt gacgtgcagc tagttttatt 
      841 ttaaagacaa atgtcagaga ggctcatcat attttccccc ctcttctata tttggagctt 
      901 atttattgct aagaagctca ggctcctggc gtcaatttat cagtaggctc caaggagaag 
      961 agaggagagg agaggagagc tgaacaggga gccacgtctt ttcctgggag ggctgctatc 
     1021 taagtcgggg ctgcaggttg gagattttta aggaagtgga aattggcaat tggctttgtg 
     1081 tgtctgtggt ttttggggag ggggactaca aagaggggct aactccctct ccctattctc 
     1141 taaggttgga ccacagggat gaggttgtga gatacaaaga taaaggaggg atggggaaca 
     1201 ctatgatgtg gtatttttct tttctgtttt ttctttttga taatatctat ccttggctaa 
     1261 ggggagggcg gagttcagcg gcggaaataa agcgagcagt ggctggtgcg aaccgactcc 
     1321 cggctctaag ccttacttgc ggtggccgga cttgtctgtg gctgaagccg agcccgggct 
     1381 ctgactctca cgtctgcact ggaggtgcgg aaacctggac tggggttcac cagccacata 
     1441 ctggctgctc tggttgttct ctcctcttcc ttcttctatt ctaaccaaac aaaacccaac 
     1501 tcgatgcttg tgccaggcct tggccagttt ggacggtgat ggaaacattt ctggatttta 
     1561 gcgtctaagg gagcactcaa gggctgtaag gtttgctatc actgtcccaa cactgcagag 
     1621 accttgaagg ttcagtgtgg gatctgtaga acccatggtt tgcggtgaca ctcagagggg 
     1681 atctttggga gatttataga gccggttctt agcgctttgt gggttcagag gctggctgca 
     1741 gtgtttatga agaggggcag tgggctgggg acactgctgg ggttatggct gtagtgaggt 
     1801 ccatgtgtac ttgttccttg gcatgtgtct gactctgtgt tgctgctgca gtacagtggg 
     1861 caggggcacg gttgcttgga ctgggctgcc cgatcaccct ccgaggactg ggagtcccga 
     1921 gtccagctta gggggagtgg gggcgcgacc cccaacccag aaaccttcac ttgaccgctc 
     1981 aagttcgcgg cagcagggcg ggccgcgccg aatctcggcg tgcgcggagc ggggagatgc 
     2041 aggcgagcgc cagagcccgg gctcgggggc cctgcgccgg ggagaggagc cgggacccac 
     2101 cggcggagcc gaaaacaagt gtattcatat tcaaacaaac ggaccaattg caccaggcgg 
     2161 ggagagggag catccaatcg gctggcgcga ggccccggcg ctgctttgca taaagcaata 
     2221 ttttgtgtga gagcgagcgg tgcatttgca tgttgcggag tgattagtgg gtttgaaaag 
     2281 ggaaccgtgg ctcggcctca tttcccgctc tggttcaggc gcaggaggaa gtgttttgct 
     2341 ggaggatggc gcgccgaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa 
     2401 gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt 
     2461 tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc 
     2521 actcccaggc ggccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 
     2581 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 
     2641 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 
     2701 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcgccc 
     2761 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 
     2821 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 
     2881 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 
     2941 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca 
     3001 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg 
     3061 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 
     3121 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 
     3181 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 
     3241 tggacgagct gtacaagtaa gcggccgcga tcgctcaacc tctggattac aaaatttgtg 
     3301 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 
     3361 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 
     3421 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 
     3481 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 
     3541 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 
     3601 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 
     3661 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 
     3721 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 
     3781 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 
     3841 ccctttgggc cgcctccccg cgatcgcaga catgataaga tacattgatg agtttggaca 
     3901 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 
     3961 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 
     4021 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa 
     4081 atgtggtagt cgaaattccc gataaggatc ttcctagagc atggctacgt agataagtag 
     4141 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct 
     4201 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 
     4261 gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa ttcactggcc 
     4321 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 
     4381 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 
     4441 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg 
     4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 
     4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
     4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
     4681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
     4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
     4801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
     4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 
     4921 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
     4981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
     5041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
     5101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
     5161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
     5221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
     5281 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
     5341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
     5401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
     5461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
     5521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
     5581 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
     5641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
     5701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
     5761 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
     5821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
     5881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
     5941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     6001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
     6061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
     6121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 
     6181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 
     6241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 
     6301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 
     6361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 
     6421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
     6481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
     6541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
     6601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
     6661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
     6721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
     6781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 
     6841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 
     6901 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 
     6961 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 
     7021 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 
     7081 atgattacgc cagatttaat taagg 
//
