LOCUS       pEMS1930        4855 bp    DNA     circular SYN 15-FEB-2012
DEFINITION  Ple257 [cloned in pJ204].
ACCESSION   DNA2.0 Id: 75843
SOURCE      Synthetic.
  ORGANISM  Synthetic
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617879903|
COMMENT     VNTDBDATE|617879903|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1930|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "DNA 'pEMS1930'" 1) 
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COMMENT                          "Ple257 [cloned in pJ204]" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 02/15/12 09:18AM" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/DNA2.0\">DNA2.0</a>; <a href=\"ncbilink:dnarna/Id:\">Id:</a>; <a href=\"ncbilink:dnarna/75843\">75843</a>" 
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FEATURES             Location/Qualifiers
     source          1..4855
                     /organism=synthetic
                     /vntifkey="98"
     gene            complement(1235..3394)
                     /gene=Ple257
                     /vntifkey="60"
     misc_feature    complement(1235..3394)
                     /product=Ple257
                     /vntifkey="21"
                     /label=Ple257
     misc_feature    complement(4011..4814)
                     /product=pUC_ori
                     /vntifkey="21"
                     /label=pUC_ori
     misc_feature    3726..3839
                     /product=rpn txn terminator
                     /vntifkey="21"
                     /label=rpn\txn\terminator
     misc_feature    3419..3719
                     /product=bla txn terminator
                     /vntifkey="21"
                     /label=bla\txn\terminator
     misc_feature    complement(1038..1212)
                     /product=rrnB1 B2 T1 txn terminator
                     /vntifkey="21"
                     /label=rrnB1\B2\T1\txn\terminator
     misc_feature    complement(3510..3535)
                     /product=pTF3
                     /vntifkey="21"
                     /label=pTF3
     misc_feature    1113..1129
                     /product=pTR
                     /vntifkey="21"
                     /label=pTR
     misc_feature    complement(8..856)
                     /product=Ampicilin
                     /vntifkey="21"
                     /label=Ampicilin
BASE COUNT     1252 a      1238 c      1183 g      1182 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgccctttt gacgggcgcg cctgacagcc gcgttctacg 
     1261 cgaggacctg ccccagagtt taaatgtcaa tgataagaaa agagggtgct caggcaggcg 
     1321 ctaactttcc ttaatatcca cgccagcgcc gtcctcattg gctgcccggc ccgcgtgacg 
     1381 tcatggcggc tagagttggg cacagctctg cgccgactag ttttccggcc gggcgggagc 
     1441 ctgcttctcc ccacccaggg tccggccggc tccaacccct gctttggcct tccttggccc 
     1501 ggcctgtgac tgctctagcc cggcccggtc cagcctgggc cgccgtcctg tatggcgtag 
     1561 agtcgatccc caggaaacta gtcttgccga gtgcgctcct ccgcaccttc gggcactagg 
     1621 gccccaagat ctctgccctt aggctggcca gctacctctc tgggtaatca ccctgtcccc 
     1681 tcctccgatc cccaagacgg agcctggaag aaagggaggg aggaaaaggg aggaaaaaaa 
     1741 acaagttaca tggaattagt tggaagcggg cgcattcccc agcgaccgca gccattaaca 
     1801 ttataatctc aaacgccatt ttttatgtaa cactattgtt agtaaatcaa ctcctcaggt 
     1861 cctcagagac gctgacccct ggcaattcag tgaaagtgta attatctccc cgaatcttgg 
     1921 acagatttaa gagattacat ttcaactaaa tctatatcaa tgctaattcc ctctctcccc 
     1981 ccagcctgca ggtgctaggc acgggaaatg gcgtcaggcc ccagcagaac gcttcacaaa 
     2041 gaggagaggg ctagctgtgt acagggccaa aagaaatgaa gccccacata aaattacacc 
     2101 tttttttttt tgctacaaaa cctggttctt atatagcaaa gttaaacaaa gcagctatgc 
     2161 taaattagcg ttacataaag gcgagtttaa tgctcggtta cagtcaactg gtgtatctct 
     2221 gatggctaat tgtctatcaa aattaaagag aaaagaacaa atgattcgtt ataagaaggt 
     2281 gttaaaagcc tcaacaaaag gacatttcac aaatataata tcggcagctc ccggattgcc 
     2341 tgcggggttg ttcaggcccc gggcctgcac agctatgaga agtggggtgc agggtgctat 
     2401 ttgcccagca aaagctcatt tcctcccaaa tgagtataat gaaaacccga gaggcgatat 
     2461 tagtgtaaag atcgggatta gtccaaatag gagccgcgga cttgggcttc aaaggcgacc 
     2521 cctactcttt ttgcccagtc tgggggcgga gggcgctcga agggatactt caaggacacc 
     2581 acatccaact tgtcgcagcc ccacgttcag gcacaggagg gagcgaacat tgggcagagg 
     2641 gtaatttcaa caaaggacgg caggctccgg ggatcccggc ttttccctga gttctctggg 
     2701 ttggaaggag agggtccggg ctctgtcgga ggagtgggta cagcccagca catgcttttc 
     2761 tgacgcgcag cgctggcctg gggagtgggt gcagctgctg cagccagccc ccaccccgcc 
     2821 ccaggctggg aaggacgcag agtataatac gacggccccc ttccccaagg ggacactaga 
     2881 tccagagctc caggcatatt tgttgttcaa agttaattat acctgaaagc aaccaaaatg 
     2941 cgcacgccag gcccacggag gccgattgcc gaccagcgta gccggcccgc agcgccagag 
     3001 aagccgctca ctccggcgga gtctctgcaa gagaatctga ggctggggaa taaaagccga 
     3061 aactaaatca ggcagcaaga gtaacaaacc atctggaaaa tggacccacg gagccgcaag 
     3121 aatgtacttt ttttttttat ccttgacaac ttgtgtccta aagaaagtgt attgaagata 
     3181 attgaaatga ttacaattca ttgctgcctg actttgaata tgaacagtca cataatgaca 
     3241 tccccgccac acaacaacag tttaatttcc attatccgac ttcgggatcc tagcgccagc 
     3301 cgccggggca acgtggcgct aatgcagaaa tcttggctcc tgcctgcatt attcatttcg 
     3361 gggacgtggg gatgggatag gagtggagac gcgtttcagc gaagggcgac acaaaattta 
     3421 ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc 
     3481 gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta 
     3541 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata 
     3601 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt 
     3661 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag 
     3721 gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc 
     3781 cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg 
     3841 gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa 
     3901 aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc 
     3961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     4021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     4081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     4141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     4201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     4261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     4321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     4381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     4441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     4501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg 
     4561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 
     4621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     4681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     4741 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg 
     4801 gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg ctttt 
//
