LOCUS       pEMS1931        4808 bp    DNA     circular SYN 15-FEB-2012
DEFINITION  Ple258 [cloned in pJ204].
ACCESSION   DNA2.0 Id: 75844
SOURCE      Synthetic.
  ORGANISM  Synthetic
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|617879991|
COMMENT     VNTDBDATE|617879991|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS1931|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Ple258 [cloned in pJ204]" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/DNA2.0\">DNA2.0</a>; <a href=\"ncbilink:dnarna/Id:\">Id:</a>; <a href=\"ncbilink:dnarna/75844\">75844</a>" 
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FEATURES             Location/Qualifiers
     source          1..4808
                     /organism=synthetic
                     /vntifkey="98"
     gene            complement(1235..3346)
                     /gene=Ple258
                     /vntifkey="60"
     misc_feature    complement(1235..3346)
                     /product=Ple258
                     /vntifkey="21"
                     /label=Ple258
     misc_feature    complement(3964..4767)
                     /product=pUC_ori
                     /vntifkey="21"
                     /label=pUC_ori
     misc_feature    3679..3792
                     /product=rpn txn terminator
                     /vntifkey="21"
                     /label=rpn\txn\terminator
     misc_feature    3372..3672
                     /product=bla txn terminator
                     /vntifkey="21"
                     /label=bla\txn\terminator
     misc_feature    complement(1038..1212)
                     /product=rrnB1 B2 T1 txn terminator
                     /vntifkey="21"
                     /label=rrnB1\B2\T1\txn\terminator
     misc_feature    complement(3463..3488)
                     /product=pTF3
                     /vntifkey="21"
                     /label=pTF3
     misc_feature    1113..1129
                     /product=pTR
                     /vntifkey="21"
                     /label=pTR
     misc_feature    complement(8..856)
                     /product=Ampicilin
                     /vntifkey="21"
                     /label=Ampicilin
BASE COUNT     1310 a      1123 c      1009 g      1366 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgccctttt gacgggcgcg cctgacagcc gcgttctacg 
     1261 cgaggacctg ccccagagtt taaatgtcaa tgataagaaa agagggtgct caggcaggcg 
     1321 ctaactttcc ttaatatcca cgccagcgcc gtcctcattg gctgcccggc ccgcgtgacg 
     1381 tcatggcggc tagagttggg cacagctctg cgccgactag ttttccggcc gggcgggagc 
     1441 ctgcttctcc ccacccaggg tccggccggc tccaacccct gctttggcct tccttggccc 
     1501 ggcctgtgac tgctctagcc cggcccggtc cagcctgggc cgccgtcctg tatggcgtag 
     1561 agtcgatccc caggaaacta gtcttgccga gtgcgctcct ccgcaccttc gggcactagg 
     1621 gccccaagat ctctgccctt aggctggcca gctacctctc tgggtaatca ccctgtcccc 
     1681 tcctccgatc cccaagtaag attaaatgaa agaagtaaat cctctttgaa ataagcaaat 
     1741 gtcaggttga ccatttaaat acacagtact ttgcttatgt actatcagaa tggaaattta 
     1801 tctcatcttt aaaccttgca atttctgtta ttcatataat tgagaaagtc ctagtttata 
     1861 tcactgaaat cttgaataga tgagttctaa caaaaattga cttgtatcat tttagtttca 
     1921 gatatttaga taaaaaggct tagtctttta tttatagata aaaggaaatc tgaccttttg 
     1981 attaagaaat attgtcagta gcctttataa atgcttgttc atctgagggt ccatgacagg 
     2041 agtactcttc ccagttgagt ttatcctaaa aatgaaatga taagagcctg ctggttatta 
     2101 tccatgattc ttcttaatgg gggagccaca tggttattct tcatctccca cttgttctca 
     2161 ccctttacta ccaacccagc cacaccccac ccccacccca acattgttca gtactcagca 
     2221 caatgaatca ttttcaagtt gactcattgt gaactgtatc ccagctgttg gaatgtctgg 
     2281 cagacatttt tgtttgggtg aagaataact ggctgaaacc caacctggat gagtttgata 
     2341 taatggtctt tggctaaaga aatattgaga ggaacatgtt tatggcctct gaagcccctg 
     2401 ttattcctgg agtaggacca tcccctgtgg cacagaactg aaagtaagga atcaatctgg 
     2461 attattttct caccattgaa tgaaaaattg ccttagaaaa aataggcatt tccaaactat 
     2521 gtttttctat acttagtgtg cccacgtgat tgaacatatt aaagacaatt aagaataatt 
     2581 atttttactg attcttttta acaacactga tccaaaggct gaaacattcc ttgtataatg 
     2641 ctgaaacatt ctttatataa cacacattcc aacttatgaa ggagtttcaa atactgctct 
     2701 aggtgtgagt taaccagtca tttcaacaga atgtttggtg acacaggcta ctatgtgttt 
     2761 cataatacca agtcaggtaa cattgaccag ccctctttaa tttttcattc ttaggccatc 
     2821 tttataaagg gacttaagaa acaagaatat tagtaagctc agttgtaatc atgttcccag 
     2881 aaaatgaaaa tcttttatta tatttgacct ctttctaaca ggggatatag caggctgata 
     2941 aattttcttg actgttttat atttcccatt ctcttcttca aagattagtt ttaaactggt 
     3001 gggcattacc attgtataaa atgagagttt caaaattgca aaattttaca aaaaacattt 
     3061 tcagcttatc tagatctgcc aagcaaaaaa ttgtctaaga catcatagca tataatcaca 
     3121 gttttctaat ctaccaccct gtcaggggct aaaaggaata ctagatttcc agtaattaaa 
     3181 gaattaaaaa aaaaaaccct accagatatt tgatgattat attcttatag ccaggagatt 
     3241 cttagtattt tagaactttt gctacttaat aactggacat actatgttta taaacatatt 
     3301 aacctattgt tttgatctgg caaaatgtga catttattaa acgcgtattc agcgaagggc 
     3361 gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag tttgtattat 
     3421 attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta 
     3481 ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa 
     3541 atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta 
     3601 tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca 
     3661 agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa actcccccca 
     3721 taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc 
     3781 agaaccgccc agggggcccg agcttaagac tggccgtcgt tttacaacac agaaagagtt 
     3841 tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc ttagtttgat gcctggcagt 
     3901 tccctactct cgccttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     3961 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     4021 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     4081 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     4141 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     4201 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     4261 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     4321 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     4381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     4441 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     4501 ttgaagtggt gggctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 
     4561 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     4621 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     4681 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgcgcgta 
     4741 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct 
     4801 ctgctttt 
//
