LOCUS       pSAH0016                4271 bp    DNA     circular UNA 10-JUN-2016
DEFINITION  TIA2GS-301-pSB4X5 for AddGene.
ACCESSION   urn.local.Stefan.1465547616104.12
VERSION     urn.local.Stefan.1465547616104.12
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..2240
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of TIA2GS-016-pSB4X5 - Fragment 2 -
                     Digestion of TIA2G-016-pSB4X5 - Fragment 2 (concatenated
                     sequence 1) extraction (concatenated sequence 1)"
     misc_feature    complement(1..2037)
                     /label="BBa I50041"
     misc_difference 36
                     /label="Mut: difference from canonical pSC101 sequence in
                     Genbank"
     polyA_signal    complement(76..91)
                     /label="putative polyA"
     misc_difference 126
                     /label="Mut: difference from canonical pSC101 sequence in
                     Genbank"
     CDS             complement(202..1152)
                     /gene="repA"
                     /label="repA CDS"
     misc_difference 288
                     /label="Mut: difference from canonical pSC101 sequence in
                     Genbank"
     misc_difference 383
                     /label="Mut: difference from canonical pSC101 sequence in
                     Genbank"
     RBS             complement(1154..1159)
                     /label="RBS"
     misc_feature    complement(1154..1176)
                     /note="Geneious type: misc"
                     /gene="inverted repeat"
                     /label="inverted repeat misc"
     RBS             complement(1156..1168)
                     /label="RBS"
     misc_feature    complement(1179..1200)
                     /note="Geneious type: misc"
                     /gene="inverted repeat"
                     /label="inverted repeat misc"
     misc_difference complement(1219..1224)
                     /label="Mut: Mutated SpeI site"
     misc_feature    complement(1250..1269)
                     /note="Geneious type: misc"
                     /gene="direct repeat"
                     /label="direct repeat misc"
     misc_feature    complement(1282..1301)
                     /note="Geneious type: misc"
                     /gene="direct repeat"
                     /label="direct repeat misc"
     misc_feature    complement(1303..1322)
                     /note="Geneious type: misc"
                     /gene="direct repeat"
                     /label="direct repeat misc"
     misc_feature    complement(1321..1403)
                     /note="Geneious type: misc"
                     /gene="AT rich region"
                     /label="AT rich region misc"
     misc_feature    complement(1534..1901)
                     /note="Geneious type: misc"
                     /gene="par region"
                     /label="par region misc"
     misc_feature    complement(1800..1817)
                     /note="Geneious type: misc"
                     /gene="inverted repeat"
                     /label="inverted repeat misc"
     misc_feature    complement(1823..1846)
                     /note="Geneious type: misc"
                     /gene="inverted repeat"
                     /label="inverted repeat misc"
     misc_feature    complement(1857..1873)
                     /note="Geneious type: misc"
                     /gene="inverted repeat"
                     /label="inverted repeat misc"
     misc_feature    complement(1876..1880)
                     /note="Geneious type: misc"
                     /gene="Tn3 integration site"
                     /label="Tn3 integration site misc"
     misc_feature    complement(1886..1899)
                     /note="Geneious type: misc"
                     /gene="inverted repeat"
                     /label="inverted repeat misc"
     stem_loop       complement(1963..1992)
                     /label="terminator"
     primer_bind     1973..1991
                     /label="GATC_std_pQE-RP"
     misc_feature    complement(2038..2044)
                     /label="BBa G0006"
     misc_feature    complement(2045..2116)
                     /label="BBa B0053"
     stem_loop       2053..2087
                     /label="stem loop"
     misc_feature    complement(2117..2136)
                     /label="BBa G00100"
     misc_feature    complement(2137..2214)
                     /label="BBa B0055"
     misc_feature    complement(2164..2202)
                     /note="Geneious type: misc"
                     /gene="identical to bacteriophage T3"
                     /label="identical to bacteriophage T3 misc"
     stem_loop       complement(2172..2193)
                     /label="stem loop from bacteriophage T3"
     misc_feature    complement(2215..2226)
                     /label="BBa B0042"
     misc_feature    complement(2215..2217)
                     /label="stop: stop codon (-1 frame)"
     misc_feature    2216..2218
                     /label="stop: stop codon (+2 frame)"
     misc_feature    complement(2219..2221)
                     /label="stop: stop codon (-3 frame)"
     misc_feature    2220..2222
                     /label="stop: stop codon (+3 frame)"
     misc_feature    complement(2223..2225)
                     /label="stop: stop codon (-2 frame)"
     misc_feature    2224..2226
                     /label="stop: stop codon (+1 frame)"
     misc_feature    2227..2231
                     /note="Geneious type: misc"
                     /gene="Topoisomerase I recognition site"
                     /label="Topoisomerase I recognition site misc"
     misc_feature    complement(2227..2239)
                     /label="BBa B0043"
     misc_feature    2231
                     /note="Geneious type: misc"
                     /gene="Generated T overhang"
                     /label="Generated T overhang misc"
     misc_feature    2232..2234
                     /note="Geneious type: misc"
                     /gene="Arbitrary sequence (could be any bases)"
                     /label="Arbitrary sequence (could be any bases) misc"
     misc_feature    complement(2235..2239)
                     /note="Geneious type: misc"
                     /gene="Nt.BstNB I recognition site"
                     /label="Nt.BstNB I recognition site misc"
     misc_feature    2240
                     /note="Geneious type: misc"
                     /gene="Prefix"
                     /label="Prefix misc"
     misc_feature    2240
                     /label="BBa G00000"
     primer_bind     2241..2317
                     /label="TIA-301 fw"
     misc_feature    <2241..2245
                     /note="Geneious type: polylinker"
                     /label="EcoRI"
     misc_feature    2241..2244
                     /note="Geneious type: ligation"
                     /label="Ligation"
     misc_feature    order(2241..>2317,<2241..>2317)
                     /note="Geneious type: Concatenated sequence"
                     /label="Ligated sequences (concatenated sequence 1)"
     misc_feature    <2241..2244
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of pSB4K5 (modified) - Fragment 1
                     extraction 2 (concatenated sequence 2)"
     misc_feature    <2241..>2244
                     /note="Geneious type: misc"
                     /gene="Prefix"
                     /label="Prefix misc"
     misc_feature    complement(<2241..>2244)
                     /label="BBa G00000"
     misc_feature    2245..>2317
                     /note="Geneious type: Concatenated sequence"
                     /label="Ligated sequences extraction 2 (concatenated
                     sequence 1)"
     misc_feature    2245..2317
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of TIA2G-301-pSB4X5 - Fragment 1
                     (concatenated sequence 2)"
     misc_feature    2246..2254
                     /note="Geneious type: motif"
                     /label="proximal UP (Estrem et al 1999)"
     promoter        2246..2291
                     /label="conI w UP"
     misc_feature    2245^2246
                     /note="Geneious type: Editing History Deletion"
                     /label="AATTGTGAGCGCTCACAATTCC"
     misc_feature    2254^2255
                     /note="Geneious type: Editing History Deletion"
                     /label="T"
     misc_feature    <2255..2291
                     /label="conI promoter"
     misc_feature    2290^2291
                     /note="Geneious type: Editing History Deletion"
                     /label="GATGACGTCACTAGATGACGTCACTAGATGACGTCACTAGATGACGTCAT
                     CTAG"
     misc_feature    2292..>2317
                     /note="Geneious type: Editing History Insertion"
                     /label
     misc_signal     2299..2300
                     /label="LacO center"
     misc_feature    2317
                     /note="Geneious type: polylinker"
                     /label="XbaI"
     misc_feature    <2318..2322
                     /note="Geneious type: polylinker"
                     /label="XbaI"
     misc_feature    2318..2321
                     /note="Geneious type: ligation"
                     /label="Ligation"
     misc_feature    order(<2318..2995,4110..>2240)
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of TIA2G-016-pSB4X5 - Fragment 2
                     (concatenated sequence 1)"
     misc_feature    2322..4271
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of TIA2GS-016-pSB4X5 - Fragment 2
                     (concatenated sequence 3)"
     misc_feature    2335..2994
                     /note="Geneious type: Marker"
                     /gene="CAT marker"
                     /label="CAT marker"
     misc_feature    complement(2970..2985)
                     /note="Geneious type: primer_bind_reverse"
                     /label="GATC_std_pTeSp-2"
     misc_feature    2995
                     /note="Geneious type: polylinker"
                     /label="HindIII"
     misc_feature    <2996..3000
                     /note="Geneious type: Editing History Insertion"
                     /label
     misc_feature    2996..2999
                     /note="Geneious type: ligation"
                     /label="Ligation"
     misc_feature    3000..4105
                     /note="Geneious type: Concatenated sequence"
                     /label="Digestion of aadA for TIA2 reverse resistance -
                     Fragment 2 (reversed) (concatenated sequence 2)"
     misc_feature    complement(3004..4011)
                     /note="Geneious type: Marker"
                     /label="aadA SpR Marker"
     misc_feature    4105..>4109
                     /note="Geneious type: Editing History Insertion"
                     /label
     misc_feature    4106..4109
                     /note="Geneious type: ligation"
                     /label="Ligation"
     misc_feature    4110
                     /note="Geneious type: misc"
                     /gene="Suffix"
                     /label="Suffix misc"
     misc_feature    4110
                     /label="BBa G00001"
     misc_feature    4111..4115
                     /note="Geneious type: misc"
                     /gene="Nt.BstNB I recognition site"
                     /label="Nt.BstNB I recognition site misc"
     misc_feature    complement(4111..4123)
                     /label="BBa B0044"
     misc_feature    4116..4118
                     /note="Geneious type: misc"
                     /gene="Arbitrary sequence (could be any bases)"
                     /label="Arbitrary sequence (could be any bases) misc"
     misc_feature    complement(4119..4123)
                     /note="Geneious type: misc"
                     /gene="Topoisomerase I recognition site"
                     /label="Topoisomerase I recognition site misc"
     misc_feature    4119
                     /note="Geneious type: misc"
                     /gene="Generated T overhang"
                     /label="Generated T overhang misc"
     misc_feature    complement(4124..4135)
                     /label="BBa B0042"
     misc_feature    complement(4124..4126)
                     /label="stop: stop codon (-1 frame)"
     misc_feature    4125..4127
                     /label="stop: stop codon (+2 frame)"
     misc_feature    complement(4128..4130)
                     /label="stop: stop codon (-3 frame)"
     misc_feature    4129..4131
                     /label="stop: stop codon (+3 frame)"
     misc_feature    complement(4132..4134)
                     /label="stop: stop codon (-2 frame)"
     misc_feature    4133..4135
                     /label="stop: stop codon (+1 frame)"
     misc_feature    complement(4136..4204)
                     /label="BBa B0054"
     misc_feature    4139..4178
                     /note="Geneious type: misc"
                     /gene="identical to E. coli MG1655 between tonB and yciA"
                     /label="identical to E. coli MG1655 between tonB and yciA
                     misc"
     stem_loop       4146..4177
                     /label="stem loop"
     misc_feature    complement(4205..4224)
                     /label="BBa G00102"
     misc_feature    complement(4225..4265)
                     /label="BBa B0062"
     stem_loop       4238..4257
                     /label="stem loop"
     misc_feature    complement(4266..>4271)
                     /label="BBa G0005"
ORIGIN      
        1 gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc
       61 tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt
      121 gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata
      181 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
      241 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat
      301 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
      361 tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt
      421 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
      481 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
      541 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
      601 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
      661 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
      721 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
      781 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
      841 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
      901 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
      961 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     1021 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     1081 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     1141 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     1201 ggctagtcaa tgataattac atgtcctttt cctttgagtt gtgggtatct gtaaattctg
     1261 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc
     1321 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     1381 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     1441 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     1501 ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt
     1561 ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca
     1621 cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa
     1681 ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg
     1741 gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc
     1801 cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt
     1861 aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca
     1921 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1981 gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacagctc
     2041 tagctccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     2101 aagattactt cgcgtttgcc acctgacgtc taagaaaagg aatattcagc aatttgcccg
     2161 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaattagtt
     2221 agttagccct tagtgactcg aattcaaaaa aatgcttgac aattaatcat cggctcgtat
     2281 aatgtgtgga attgtgagcg ctcacaattc cacacatcta gaaaggaagc taaaatggag
     2341 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     2401 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     2461 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     2521 cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg
     2581 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     2641 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     2701 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     2761 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     2821 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     2881 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc
     2941 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaaagctt
     3001 ttatttacct attaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     3061 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     3121 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     3181 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     3241 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     3301 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     3361 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     3421 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     3481 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     3541 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     3601 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     3661 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     3721 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     3781 cgcttccctc atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3841 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     3901 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     3961 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtaagcgca ttttagcttc
     4021 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     4081 atggtgaaag ttggaacctc ttacctgcag gagtcactaa gggttagtta gttagattag
     4141 cagaaagtca aaagcctccg accggaggct tttgactaaa acttcccttg gggttatcat
     4201 tggggctcac tcaaaggcgg taatcagata aaaaaaatcc ttagctttcg ctaaggatga
     4261 tttctgctag t
//
