LOCUS       pBAD-Kan_LacI-Hi        5026 bp    DNA     circular UNA 31-JUL-2014
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local.Stefan.1365063780534.54
VERSION     urn.local.Stefan.1365063780534.54
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      1..620
                     /gene="pBR322 ori"
                     /note="REP_ORIGIN REP_ORIGIN REP_ORIGIN REP_ORIGIN
                     REP_ORIG IN REP_ORIG IN REP_ORIG IN REP_ORIG IN REP_ORIG
                     IN REP_ORIG IN REP_ORIG REP_ORIGIN"
                     /note="REP_ORIGIN"
                     /label="pBR322 ori rep origin"
     misc_feature    complement(95..112)
                     /note="Geneious type: primer_bind_reverse"
                     /label="o_pBR322ori_r"
     primer_bind     263..287
                     /label="o_F1-fw"
     primer_bind     434..453
                     /label="MluI sequencing primer"
     primer_bind     705..724
                     /label="O51 VR2 reverse"
     primer_bind     705..724
                     /label="VR-2 Reverse Primer"
     primer_bind     979..997
                     /label="Invitrogen Interference2"
     primer_bind     1020..1038
                     /label="GATC_std_pGEX-3"
     primer_bind     1191..1215
                     /label="pf_AraC_DI"
     CDS             complement(1195..2073)
                     /gene="AraC"
                     /note="CDS"
                     /note="CDS"
                     /codon_start=0
                     /label="AraC CDS"
     misc_feature    2103..2118
                     /note="Geneious type: prot_bind"
                     /gene="araO1"
                     /label="araO1 prot bind"
     misc_feature    2260..2271
                     /note="Geneious type: prot_bind"
                     /gene="Ara O2"
                     /label="Ara O2 prot bind"
     misc_feature    2302..2315
                     /note="Geneious type: prot_bind"
                     /gene="CAP"
                     /label="CAP prot bind"
     primer_bind     2307..2326
                     /label="GATC_std_pBAD-FP"
     misc_feature    2312..2350
                     /note="Geneious type: prot_bind"
                     /gene="Ara 1|2"
                     /label="Ara 1|2 prot bind"
     promoter        2347..2379
                     /gene="Ara"
                     /note="PROMOTER PROMOTER PROMOTER PROMOTER PROMOTER
                     PROMOTE R PROMOTE R PROMOTE R PROMOTER PROMOTER"
                     /note="PROMOTER"
                     /label="Ara promoter"
     primer_bind     2361..2388
                     /label="pBAD ColPCR fw"
     misc_feature    2416..2432
                     /note="Geneious type: prot_bind"
                     /gene="T7 RBS"
                     /label="T7 RBS prot bind"
     misc_feature    2434..2437
                     /note="Geneious type: ligation"
                     /label="Ligation"
     misc_feature    <2434..2438
                     /note="Geneious type: polylinker"
                     /label="XbaI"
     misc_feature    2439
                     /note="Geneious type: Editing History Replacement"
                     /label="G>A"
     misc_feature    3519..>3523
                     /note="Geneious type: polylinker"
                     /label="PstI"
     misc_feature    3520..3523
                     /note="Geneious type: ligation"
                     /label="Ligation"
     CDS             3531..3548
                     /gene="His"
                     /note="GENE GENE GENE GENE GENE GENE GENE GENE"
                     /note="GENE"
                     /label="His CDS"
     misc_feature    complement(3550..3573)
                     /note="Geneious type: primer_bind_reverse"
                     /label="pBAD ColPCR rv"
     misc_feature    complement(3604..3623)
                     /note="Geneious type: primer_bind_reverse"
                     /label="GATC_std_pTrcHis-RP"
     misc_feature    complement(3611..3633)
                     /note="Geneious type: primer_bind_reverse"
                     /label="Eurofins-pTrcHis_rev"
     terminator      3654..3811
                     /gene="rrnB"
                     /note="TERMINATOR TERMINATOR TERMINATOR TERMINATOR
                     TERMINAT OR TERMINAT OR TERMINATOR TERMINATOR"
                     /note="TERMINATOR"
                     /label="rrnB terminator"
     misc_feature    complement(3696..3715)
                     /note="Geneious type: primer_bind_reverse"
                     /label="GATC_std_pTI2-1-2"
     terminator      3777..3820
                     /gene="rrnB_T1"
                     /note="TERMINATOR TERMINATOR TERMINATOR TERMINATOR
                     TERMINAT OR TERMINAT OR TERMINATOR TERMINATOR"
                     /note="TERMINATOR"
                     /label="rrnB T1 terminator"
     misc_feature    complement(3826..3844)
                     /note="Geneious type: primer_bind_reverse"
                     /label="GATC-pEX_3-274412"
     terminator      3952..3979
                     /gene="rrnB_T2"
                     /note="TERMINATOR TERMINATOR TERMINATOR TERMINATOR
                     TERMINAT OR TERMINAT OR TERMINATOR TERMINATOR"
                     /note="TERMINATOR"
                     /label="rrnB T2 terminator"
     misc_feature    complement(3969..3987)
                     /note="Geneious type: primer_bind_reverse"
                     /label="o_pBAD_r"
     primer_bind     complement(3969..3987)
                     /label="o_pBAD_r"
     primer_bind     3988..4004
                     /label="o_KanR_f"
     primer_bind     4005..4023
                     /label="pUC18_r"
     CDS             complement(4079..4894)
                     /label="KanR"
     misc_feature    complement(4831..4850)
                     /note="Geneious type: primer_bind_reverse"
                     /label="sf_bb_3_1_n"
     primer_bind     4970..4987
                     /label="o_pBAD_f"
     misc_feature    complement(4979..4996)
                     /note="Geneious type: primer_bind_reverse"
                     /label="o_Kanr_r"
     primer_bind     4997..5014
                     /label="o_pBAD_f"
     misc_feature    complement(5006..5023)
                     /note="Geneious type: primer_bind_reverse"
                     /label="o_Kanr_r"
ORIGIN      
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      841 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
      901 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
      961 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     1021 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
     1081 ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt caaatggacg aagcagggat
     1141 tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga
     1201 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
     1261 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
     1321 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat
     1381 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga
     1441 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
     1501 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
     1561 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
     1621 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
     1681 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
     1741 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
     1801 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
     1861 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
     1921 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
     1981 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
     2041 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
     2101 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
     2161 ctcgctaacc aaaccgggaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
     2221 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
     2281 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
     2341 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc
     2401 tagaaataat tttgtttaac tttaagaagg agtctagaat gaaaccagta acgttatacg
     2461 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     2521 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     2581 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     2641 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     2701 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     2761 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     2821 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     2881 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     2941 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     3001 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     3061 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     3121 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     3181 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggaca
     3241 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     3301 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     3361 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     3421 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3481 agctggcacg acaggtttcc cgactggaaa gcgggcagct gcagcccggt catcatcacc
     3541 atcaccattg agtttaaacg gtctccagct tggctgtttt ggcggatgag agaagatttt
     3601 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
     3661 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     3721 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
     3781 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     3841 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
     3901 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
     3961 tcctgacgga tggccttttt gcgtttcagg tggcactttt cggggaaatg tgcgcggaac
     4021 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt
     4081 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     4141 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     4201 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     4261 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     4321 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     4381 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     4441 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     4501 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     4561 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
     4621 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     4681 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     4741 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     4801 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     4861 tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     4921 aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga
     4981 gcgtcagacc ccgtagcgtt ccactgagcg tcagaccccg tagaaa
//
