LOCUS       pKA-141corrected\BphP1        5805 bp    DNA     circular SYN  1-MAY-2018
DEFINITION  CMVd1 Flexi Vector pFC15K (HaloTag 7), complete sequence.
ACCESSION   EU332338
VERSION     EU332338.1  GI:163943735
SOURCE      CMVd1 Flexi Vector pFC15K (HaloTag 7).
  ORGANISM  CMVd1 Flexi Vector pFC15K (HaloTag 7)
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Brouette,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2007) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12269378|
COMMENT     VNTDBDATE|-12269378|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pKA-141corrected BphP1|
COMMENT     VNTAUTHORNAME|KaaG|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "CMVd1 Flexi Vector pFC15K (HaloTag 7), complete sequence." 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/EU332338\">EU332338</a>" 
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COMMENT                          "Sequence Source: CMVd1 Flexi Vector pFC15K (HaloTag 7)." 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/CMVd1+Flexi+Vector+pFC15K+(HaloTag+7)\">CMVd1 Flexi Vector pFC15K (HaloTag 7)(CMVd1 Flexi Vector pFC15K (HaloTag 7))</a>" 
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FEATURES             Location/Qualifiers
     promoter        1..121
                     /vntifkey="30"
                     /label=CMV\immediate/early\promoter;\CMVd1\starts\at\-116
                     /note="CMV immediate/early promoter; CMVd1 starts at -116"
     promoter        132..151
                     /vntifkey="30"
                     /label=T7\RNA\polymerase
                     /note="T7 RNA polymerase"
     promoter        156..175
                     /vntifkey="30"
                     /label=SP6\RNA\polymerase
                     /note="SP6 RNA polymerase"
     polyA_signal    3270..3491
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="SV40 late polyadenylation signal"
     gene            3799..4593
                     /gene="kanR"
                     /vntifkey="60"
     CDS             3799..4593
                     /gene="kanR"
                     /codon_start=1
                     /transl_table=11
                     /product="kanamycin resistance protein"
                     /protein_id="ABY48992.1"
                     /db_xref="GI:163943737"
                     /vntifkey="4"
                     /label=kanR
     rep_origin      4762..4798
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ColE1-derived origin of replication"
     misc_feature    5469..5754
                     /vntifkey="21"
                     /label=Misc_Feature_4
                     /note="Cer region"
     misc_feature    2435..3145
                     /vntifkey="21"
                     /label=mCherry
     misc_feature    200..2392
                     /vntifkey="21"
                     /label=BphP1
BASE COUNT     1186 a      1677 c      1743 g      1199 t 
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg 
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 
      121 cactagaagc ttaatacgac tcactatagg gtacgattta ggtgacacta tagaataagg 
      181 agcgatcgcc atggctagcg tggcaggtca tgcctctggc agccccgcat tcgggaccgc 
      241 cgatctttcg aattgcgaac gtgaagagat ccacctcgcc ggctcgatcc agccgcatgg 
      301 cgcgcttctg gtcgtcagcg agccggatca tcgcatcatc caggccagcg ccaacgccgc 
      361 ggaatttctg aatctcggaa gcgtgctcgg cgttccgctc gccgagatcg acggcgatct 
      421 gttgatcaag atcctgccgc atctcgatcc caccgccgaa ggcatgccgg tcgcggtgcg 
      481 ctgccggatc ggcaatccct ccacggagta cgacggtctg atgcatcggc ctccggaagg 
      541 cgggctgatc atcgagctcg aacgtgccgg cccgccgatc gatctgtccg gcacgctggc 
      601 gccggcgctg gagcggatcc gcacggcggg ctcgctgcgc gcgctgtgcg atgacaccgc 
      661 gctgctgttt cagcagtgca ccggctacga ccgggtgatg gtgtatcgct tcgacgagca 
      721 gggccacggc gaagtgttct ccgagcgcca cgtgcccggg ctcgaatcct atttcggcaa 
      781 ccgctatccg tcgtcggaca ttccgcagat ggcgcggcgg ctgtacgagc ggcagcgcgt 
      841 ccgcgtgctg gtcgacgtca gctatcagcc ggtgccgctg gagccgcggc tgtcgccgct 
      901 gaccgggcgc gatctcgaca tgtcgggctg cttcctgcgc tcgatgtcgc cgatccatct 
      961 gcagtacctg aagaacatgg gcgtgcgcgc caccctggtg gtgtcgctgg tggtcggcgg 
     1021 caagctgtgg ggcctggttg cctgtcacca ttatctgccg cgcttcatcc atttcgagct 
     1081 gcgggcgatc tgcgaactgc tcgccgaagc gatcgcgacg cggatcaccg cgcttgagag 
     1141 cttcgcgcag agccagtcgg agctgttcgt gcagcggctc gaacagcgca tgatcgaagc 
     1201 gatcacccgt gaaggcgatt ggcgcgcagc gattttcgac accagccaat cgatcctgca 
     1261 gccgctgcac gccgacggtt gcgcgctggt gtacgaagac cagatcagga ccatcggtga 
     1321 cgtaccttcc acgcaggatg ttcgcgagat cgccgggtgg ctcgatcgcc agccacgtgc 
     1381 ggcggtgacc tcgaccgcgt cgctcggtct cgacgtgccg gagctcgcgc atctgacgcg 
     1441 gatggcgagc ggcgtggtcg cggcgccgat ttcggatcat cgcggcgagt ttctgatgtg 
     1501 gttccgcccc gagcgcgtcc acaccgttac ctggggcggc gatccgaaga agccgttcac 
     1561 gatgggcgat acaccggcgg atctgtcgcc gcggcgctcc ttcgccaaat ggcatcaggt 
     1621 tgtcgaaggc acgtccgatc cgtggacggc cgccgatctc gccgcggctc gcaccatcgg 
     1681 tcagaccgtc gccgacatcg tgctgcaatt ccgcgcggtg cggacactga tcgcccgcga 
     1741 acagtacgaa cagttttcgt cccaggtgca cgcttcgatg cagccggtgc tgatcaccga 
     1801 cgccgaaggc cgcatcctgc tgatgaacga ctcgttccgc gacatgttgc cggcggggtc 
     1861 gccatccgcc gtccatctcg acgatctcgc cgggttcttc gtcgaatcga acgatttcct 
     1921 gcgcaacgtc gccgaactga tcgatcacgg ccgcgggtgg cgcggcgaag ttctgctgcg 
     1981 cggcgcaggt aatcgcccgt tgccgctggc agtgcgcgcc gatccggtga cgcgcacgga 
     2041 ggaccagtcg ctcggcttcg tgctgatctt cagcgacgct accgatcgtc gcaccgcaga 
     2101 tgccgcacgc acgcgtttcc aggaaggcat tcttgccagc gcacgtcccg gcgtgcggct 
     2161 cgactccaag tccgacctct tgcacgagaa gctgctgtcc gcgctggtcg agaacgcgca 
     2221 gcttgccgca ttggaaatta cttacggcgt cgagaccgga cgcatcgccg agctgctcga 
     2281 aggcgttcgc cagtcgatgc tgcgcaccgc cgaagtgctc ggccatctgg tgcagcacgc 
     2341 ggcgcgcacg gccggcagcg acagctcgag caatggctcg cagaacaaga aggaattcga 
     2401 tagtgctggt agtgctggta gtgctggtac tagtatggtg agcaagggcg aggaggataa 
     2461 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 
     2521 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     2581 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 
     2641 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 
     2701 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 
     2761 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 
     2821 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     2881 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 
     2941 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 
     3001 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 
     3061 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 
     3121 cggcatggac gagctgtaca agtaatctag agtcgacctg caggcatgca agctgatccg 
     3181 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta 
     3241 gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg 
     3301 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 
     3361 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 
     3421 ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta 
     3481 caaatgtggt aaaatcgaat tctaatggat cctctttgcg cttgcgtttt cccttgtcca 
     3541 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta 
     3601 cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgagcttca 
     3661 aaagaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg 
     3721 atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca 
     3781 ggatgacggt cgtttcgcat gcttgaacaa gatggattgc acgcaggttc tccggccgct 
     3841 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 
     3901 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 
     3961 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 
     4021 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 
     4081 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 
     4141 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 
     4201 caccaagcga aacatcgcat cgagcgagca cgcactcgga tggaagccgg tcttgtcgat 
     4261 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 
     4321 aaggcgcgta tgccggatgg tgaggatctc gtcgtgactc atggcgatgc ctgcttgccg 
     4381 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 
     4441 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 
     4501 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 
     4561 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 
     4621 accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     4681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     4741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     4801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     4861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     4921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     4981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     5041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     5101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     5161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     5221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     5281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     5341 aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     5401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     5461 tttatagtcc ggaaatacag gaacgcacgc tggatggccc ttcgctggga tggtgaaacc 
     5521 atgaaaaatg gcagcttcag tggattaagt gggggtaatg tggcctgtac cctctggttg 
     5581 cataggtatt catacggtta aaatttatca ggcgcgattg cggcagtttt tcgggtggtt 
     5641 tgttgccatt tttacctgtc tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta 
     5701 caattaaggg attatggtaa atccacttac tgtctgccct cgtagccatc gagataaacc 
     5761 gcagtactcc ggccacgatg cgtccggcgt agaggatcga gatct 
//
