LOCUS       pKA-142        4980 bp    DNA     circular SYN 30-JAN-2014
DEFINITION  CMVd1 Flexi Vector pFC15K (HaloTag 7), complete sequence.
ACCESSION   EU332338
VERSION     EU332338.1  GI:163943735
SOURCE      CMVd1 Flexi Vector pFC15K (HaloTag 7).
  ORGANISM  CMVd1 Flexi Vector pFC15K (HaloTag 7)
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Brouette,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2007) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|688317000|
COMMENT     VNTDBDATE|688317743|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pKA-142|
COMMENT     VNTAUTHORNAME|KaaG|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "CMVd1 Flexi Vector pFC15K (HaloTag 7), complete sequence." 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/EU332338\">EU332338</a>" 
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COMMENT                          "Sequence Source: CMVd1 Flexi Vector pFC15K (HaloTag 7)." 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/CMVd1+Flexi+Vector+pFC15K+(HaloTag+7)\">CMVd1 Flexi Vector pFC15K (HaloTag 7)(CMVd1 Flexi Vector pFC15K (HaloTag 7))</a>" 
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FEATURES             Location/Qualifiers
     promoter        1..121
                     /vntifkey="30"
                     /label=CMV\immediate/early\promoter;\CMVd1\starts\at\-116
                     /note="CMV immediate/early promoter; CMVd1 starts at -116"
     promoter        132..151
                     /vntifkey="30"
                     /label=T7\RNA\polymerase
                     /note="T7 RNA polymerase"
     promoter        156..175
                     /vntifkey="30"
                     /label=SP6\RNA\polymerase
                     /note="SP6 RNA polymerase"
     polyA_signal    2445..2666
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="SV40 late polyadenylation signal"
     gene            2974..3768
                     /gene="kanR"
                     /vntifkey="60"
     CDS             2974..3768
                     /gene="kanR"
                     /codon_start=1
                     /transl_table=11
                     /product="kanamycin resistance protein"
                     /protein_id="ABY48992.1"
                     /db_xref="GI:163943737"
                     /vntifkey="4"
                     /label=kanR
     rep_origin      3937..3973
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ColE1-derived origin of replication"
     misc_feature    4644..4929
                     /vntifkey="21"
                     /label=Misc_Feature_4
                     /note="Cer region"
     misc_feature    190..1581
                     /vntifkey="21"
                     /label=PpsR2(Cys439Ser)
     misc_feature    1660..2377
                     /vntifkey="21"
                     /label=mVenus
     misc_feature    1588..1647
                     /vntifkey="21"
                     /label=20aaLinker
     misc_feature    2383..2430
                     /vntifkey="21"
                     /label=Kras4BCT
BASE COUNT     1079 a      1380 c      1471 g      1050 t 
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg 
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 
      121 cactagaagc ttaatacgac tcactatagg gtacgattta ggtgacacta tagaataagg 
      181 agcgatcgca tggcgtcaaa gtccgttcat gccgacatca cccttctgct cgatatggag 
      241 ggtgtgattc gcgaagccac cctgtctccg acgatggcgg ccgagagcgt ggacggttgg 
      301 ctggggcgtc gctggagcga catcgccggc gccgaaggcg gcgacaaggt tcgccgcatg 
      361 gtcgaagacg cccgccgcag cggcatctcg gctttccgcc agatcaatca gcctttcccg 
      421 agcggcgtcg aaatcccgat cgaattcacc acgatgctgc tgggcgaccg caccggcatg 
      481 atcgcggtcg gcaagaacat gcaggcggtc accgagctgc attcccggct gatcgctgcg 
      541 cagcaggcga tggagcgcga ctattggcgg ttgcgtgaat tggagactcg ctaccgcctg 
      601 gtgttcgacg ctgccgccga tgcggtgatg atcgtctccg ccggcgacat gcgcatcgtc 
      661 gaagccaacc gggcggcggt gaatgcgatc agccgcgtcg agcgcggcaa tgacgacctt 
      721 gcggggcgtg atttcctcgc cgaagtggcg gctgccgatc gcgatgcggt gcgcgacatg 
      781 ctggcccagg tgcgtcagcg cggcaccgca ctcagcgtcc tcgttcatct cggccgttac 
      841 gaccgcgcct ggatgctgcg cggttcgctg atgtcgtccg agcgtcgtca ggttttcctg 
      901 ctgcacttca ccccggtgac cacgactccc gcgatcgacg acgtcgacga tgatgccgtg 
      961 ctgcgcgggc tgatcgatcg cattcccgac gggttcgtcg cactggattc ggaaggcgtc 
     1021 gttcgtcacg ccaaccaggc gtttctcgat ctggtccaga tcggctccaa gcctgcggcg 
     1081 gtcggacgat cgctgggcgt ctggatgggt cgtccgggcg ccgatctgtc cagcttgctg 
     1141 acgctgctgc ggcgctacaa gacggtgcgg ctgttccaaa cgacgatccg cggcgagctc 
     1201 ggcaccgaga ctgaagtcga ggtctcggcc gtcgacggcg aggacgacca atacatcggc 
     1261 gttctgatgc gcaatgtcgc gcgacgcctc gacgctgcgg acgaccacga tgccttgcgt 
     1321 caggcgctcg gcccgatcag caagcagctc gggcgatcct cgctgcgcaa gctggtgaag 
     1381 aacgccgtga gcattgtcga gcagcactac gtgaaggaag cgctgttgcg atccaagggc 
     1441 aatcgcacgg caactgccga actgctcgga ttgagccggc agagccttta tgcaaaactc 
     1501 aactcctacg gcttcgacga caaaggtgtc gttgcttctg ctgccgacgg tgcagagggc 
     1561 gcctcagacg acgcagagga taagcttagt gctggtggta gtgctggtgg tagtgctggt 
     1621 ggtagtgctg gtggtagtgc tggtggaccg gtcgccacca tggtgagcaa gggcgaggag 
     1681 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 
     1741 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg 
     1801 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac 
     1861 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 
     1921 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 
     1981 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 
     2041 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 
     2101 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 
     2161 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc 
     2221 cccatcggcg acggccccgt gctgctgccc gacaaccact acctaagcta ccagtccaag 
     2281 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggaacg cgtgaccgcc 
     2341 gccgggatca ctctcggcat ggacgagctg tacaaggcta gcggtaaaaa gaagaaaaag 
     2401 aagtcaaaga caaagtgtgt aattatgtaa gcggccgctt cgagcagaca tgataagata 
     2461 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 
     2521 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 
     2581 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag 
     2641 caagtaaaac ctctacaaat gtggtaaaat cgaattctaa tggatcctct ttgcgcttgc 
     2701 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg 
     2761 cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg 
     2821 gcagcgtgag cttcaaaaga attgccagct ggggcgccct ctggtaaggt tgggaagccc 
     2881 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga 
     2941 tctgatcaag agacaggatg acggtcgttt cgcatgcttg aacaagatgg attgcacgca 
     3001 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 
     3061 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 
     3121 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg 
     3181 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 
     3241 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 
     3301 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 
     3361 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgcac tcggatggaa 
     3421 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 
     3481 ctgttcgcca ggctcaaggc gcgtatgccg gatggtgagg atctcgtcgt gactcatggc 
     3541 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 
     3601 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 
     3661 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 
     3721 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 
     3781 ggttcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat 
     3841 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     3901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     3961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     4021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     4081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     4141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     4201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     4261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     4321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     4381 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     4441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     4501 gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga 
     4561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     4621 cttcacctag atccttttat agtccggaaa tacaggaacg cacgctggat ggcccttcgc 
     4681 tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc 
     4741 tgtaccctct ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gattgcggca 
     4801 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg 
     4861 ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag 
     4921 ccatcgagat aaaccgcagt actccggcca cgatgcgtcc ggcgtagagg atcgagatct 
//
