LOCUS       pKA-144        6808 bp    DNA     circular SYN 30-JAN-2014
DEFINITION  CMVd1 Flexi Vector pFC15K (HaloTag 7), complete sequence.
ACCESSION   EU332338
VERSION     EU332338.1  GI:163943735
SOURCE      CMVd1 Flexi Vector pFC15K (HaloTag 7).
  ORGANISM  CMVd1 Flexi Vector pFC15K (HaloTag 7)
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Brouette,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2007) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|688325471|
COMMENT     VNTDBDATE|688325515|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pKA-144|
COMMENT     VNTAUTHORNAME|KaaG|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "CMVd1 Flexi Vector pFC15K (HaloTag 7), complete sequence." 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/EU332338\">EU332338</a>" 
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COMMENT                          "Sequence Source: CMVd1 Flexi Vector pFC15K (HaloTag 7)." 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/CMVd1+Flexi+Vector+pFC15K+(HaloTag+7)\">CMVd1 Flexi Vector pFC15K (HaloTag 7)(CMVd1 Flexi Vector pFC15K (HaloTag 7))</a>" 
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FEATURES             Location/Qualifiers
     promoter        1..121
                     /vntifkey="30"
                     /label=CMV\immediate/early\promoter;\CMVd1\starts\at\-116
                     /note="CMV immediate/early promoter; CMVd1 starts at -116"
     promoter        132..151
                     /vntifkey="30"
                     /label=T7\RNA\polymerase
                     /note="T7 RNA polymerase"
     promoter        156..175
                     /vntifkey="30"
                     /label=SP6\RNA\polymerase
                     /note="SP6 RNA polymerase"
     polyA_signal    4273..4494
                     /vntifkey="25"
                     /label=PolyA_Signal_1
                     /note="SV40 late polyadenylation signal"
     gene            4802..5596
                     /gene="kanR"
                     /vntifkey="60"
     CDS             4802..5596
                     /gene="kanR"
                     /codon_start=1
                     /transl_table=11
                     /product="kanamycin resistance protein"
                     /protein_id="ABY48992.1"
                     /db_xref="GI:163943737"
                     /vntifkey="4"
                     /label=kanR
     rep_origin      5765..5801
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ColE1-derived origin of replication"
     misc_feature    6472..6757
                     /vntifkey="21"
                     /label=Misc_Feature_4
                     /note="Cer region"
     misc_feature    200..2392
                     /vntifkey="21"
                     /label=BphP1
     misc_feature    2435..3142
                     /vntifkey="21"
                     /label=mCherry
     misc_feature    3200..4258
                     /vntifkey="21"
                     /label=Intersect\DHPH
     misc_feature    3143..3193
                     /vntifkey="21"
                     /label=16aaLinker
     misc_feature    2399..2428
                     /vntifkey="21"
                     /label=10aa\Linker
BASE COUNT     1472 a      1924 c      2004 g      1408 t 
ORIGIN
        1 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg 
       61 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 
      121 cactagaagc ttaatacgac tcactatagg gtacgattta ggtgacacta tagaataagg 
      181 agcgatcgcc atggctagcg tggcaggtca tgcctctggc agccccgcat tcgggaccgc 
      241 cgatctttcg aattgcgaac gtgaagagat ccacctcgcc ggctcgatcc agccgcatgg 
      301 cgcgcttctg gtcgtcagcg agccggatca tcgcatcatc caggccagcg ccaacgccgc 
      361 ggaatttctg aatctcggaa gcgtgctcgg cgttccgctc gccgagatcg acggcgatct 
      421 gttgatcaag atcctgccgc atctcgatcc caccgccgaa ggcatgccgg tcgcggtgcg 
      481 ctgccggatc ggcaatccct ccacggagta cgacggtctg atgcatcggc ctccggaagg 
      541 cgggctgatc atcgagctcg aacgtgccgg cccgccgatc gatctgtccg gcacgctggc 
      601 gccggcgctg gagcggatcc gcacggcggg ctcgctgcgc gcgctgtgcg atgacaccgc 
      661 gctgctgttt cagcagtgca ccggctacga ccgggtgatg gtgtatcgct tcgacgagca 
      721 gggccacggc gaagtgttct ccgagcgcca cgtgcccggg ctcgaatcct atttcggcaa 
      781 ccgctatccg tcgtcggaca ttccgcagat ggcgcggcgg ctgtacgagc ggcagcgcgt 
      841 ccgcgtgctg gtcgacgtca gctatcagcc ggtgccgctg gagccgcggc tgtcgccgct 
      901 gaccgggcgc gatctcgaca tgtcgggctg cttcctgcgc tcgatgtcgc cgatccatct 
      961 gcagtacctg aagaacatgg gcgtgcgcgc caccctggtg gtgtcgctgg tggtcggcgg 
     1021 caagctgtgg ggcctggttg cctgtcatca ttatctgccg cgcttcatgc atttcgagct 
     1081 gcgggcgatc tgcgaactgc tcgccgaagc gatcgcgacg cggatcaccg cgcttgagag 
     1141 cttcgcgcag agccagtcgg agctgttcgt gcagcggctc gaacagcgca tgatcgaagc 
     1201 gattacccgt gaaggcgatt ggcgcgcagc gattttcgac accagccaat cgatcctgca 
     1261 gccgctgcac gccgccggtt gcgcgctggt gtacgaagac cagatcagga ccatcggcga 
     1321 cgtgccttcc acgcaggatg tgcgcgagat cgccgggtgg ctcgatcgcc agccgcgcgc 
     1381 ggcggtgacc tcgaccgcgt cgctcggtct cgacgtgccg gagctcgcgc atctgacgcg 
     1441 gatggcgagc ggcgtggtcg cggcgccgat ttcggatcat cgcggcgagt ttctgatgtg 
     1501 gttccgcccc gagcgcgtcc acaccgttac ctggggcggc gatccgaaga agccgttcac 
     1561 gatgggcgat acaccggcgg atctgtcgcc gcggcgctcc ttcgccaaat ggcatcaggt 
     1621 tgtcgaaggc acgtccgatc cgtggacggc cgccgatctc gccgcggctc gcaccatcgg 
     1681 tcagaccgtc gccgacatcg tgctgcaatt ccgcgcggtg cggacactga tcgcccgcga 
     1741 acagtacgaa cagttttcgt cccaggtgca cgcttcgatg cagccggtgc tgatcaccga 
     1801 cgccgaaggc cgcatcctgc tgatgaacga ctcgttccgc gacatgttgc cggcgggttc 
     1861 gccatccgcc gtccatctcg acgatctcgc cgggttcttc gtcgaatcga acgatttcct 
     1921 gcgcaacgtc gccgaactga tcgatcacgg ccgcgggtgg cgcggcgaag ttctgctgcg 
     1981 cggcgcaggc aaccgcccgt tgccgctggc agtgcgcgcc gatccggtga cgcgcacgga 
     2041 ggaccagtcg ctcggcttcg tgctgatctt cagcgacgct accgatcgtc gcaccgcaga 
     2101 tgccgcacgc acgcgtttcc aggaaggcat tcttgccagc gcacgtcccg gcgtgcggct 
     2161 cgactccaag tccgacctgt tgcacgagaa gctgctgtcc gcgctggtcg agaacgcgca 
     2221 gcttgccgca ttggaaatca cttacggcgt cgagaccgga cgcatcgccg agctgctcga 
     2281 aggcgtccgc cagtcgatgc tgcgcaccgc cgaagtgctc ggccatctgg tgcagcacgc 
     2341 ggcgcgcacg gccggcagcg acagctcgag caatggctcg cagaacaaga aggaattcga 
     2401 tagtgctggt agtgctggta gtgctggtac tagtatggtg agcaagggcg aggaggataa 
     2461 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 
     2521 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     2581 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 
     2641 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 
     2701 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 
     2761 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 
     2821 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     2881 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 
     2941 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 
     3001 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 
     3061 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 
     3121 cggcatggac gagctgtaca agagtgctgg tgggtccgcc ggcgggagtg ctggcggtag 
     3181 tgccggcggt agttctagag atatgttgac cccaactgaa agaaagcgac aaggatacat 
     3241 ccacgagctc attgtcaccg aggagaacta tgtgaatgac ctgcagctgg tcacagagat 
     3301 ttttcaaaaa cccctgatgg agtctgagct gctgacagaa aaagaggttg ctatgatttt 
     3361 tgtgaactgg aaggagctga ttatgtgtaa tatcaaacta ctaaaagcgc tgagagtccg 
     3421 caagaagatg tccggggaga agatgcctgt gaagatgatt ggagacatcc tgagcgcaca 
     3481 gctgccgcac atgcagccct acatccgctt ctgcagccgc cagctcaacg gggctgccct 
     3541 gatccagcag aagacggacg aggccccaga cttcaaggag ttcgtcaaaa gattggcaat 
     3601 ggatcctcgg tgtaaaggga tgccactctc tagttttata ctgaagccta tgcaacgggt 
     3661 aacaagatac ccactgatca ttaaaaatat cctggaaaac acccctgaaa accacccgga 
     3721 ccacagccac ttgaagcacg ccctggagaa ggcggaagag ctctgttccc aggtgaacga 
     3781 aggggtgcgg gagaaggaga actctgaccg gctggagtgg atccaggccc acgtgcagtg 
     3841 tgaaggcctg tctgagcaac ttgtgttcaa ttcagtgacc aattgcttgg ggccgcgcaa 
     3901 atttctgcac agtgggaagc tctacaaggc caagagcaac aaggagctgt atggcttcct 
     3961 tttcaacgac ttcctcctgc tgactcagat cacgaagcct ttggggtctt ctggcaccga 
     4021 caaagtcttc agccccaaat caaacctgca gtataaaatg tataaaacac ctattttcct 
     4081 aaatgaggtt ctagtaaaat tacccaccga cccttctgga gacgagccca tcttccacat 
     4141 ctcccacatt gaccgcgtct atactctccg agcagaaagc ataaatgaaa ggactgcctg 
     4201 ggtgcagaaa atcaaagctg cttctgaact ctacatagag actgagaaaa agaagtaagc 
     4261 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 
     4321 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 
     4381 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 
     4441 agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg 
     4501 aattctaatg gatcctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga 
     4561 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt 
     4621 agcagccctt gcgccctgag tgcttgcggc agcgtgagct tcaaaagaat tgccagctgg 
     4681 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc 
     4741 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgac ggtcgtttcg 
     4801 catgcttgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     4861 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     4921 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     4981 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     5041 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     5101 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     5161 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     5221 catcgagcga gcacgcactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     5281 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gtatgccgga 
     5341 tggtgaggat ctcgtcgtga ctcatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     5401 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     5461 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     5521 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     5581 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     5641 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     5701 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     5761 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     5821 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     5881 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     5941 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     6001 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     6061 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     6121 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     6181 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     6241 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     6301 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag 
     6361 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     6421 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttatag tccggaaata 
     6481 caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa atggcagctt 
     6541 cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt attcatacgg 
     6601 ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg gtttgttgcc atttttacct 
     6661 gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa gggattatgg 
     6721 taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagtac tccggccacg 
     6781 atgcgtccgg cgtagaggat cgagatct 
//
