LOCUS       pT2/SV_Neo-103        9500 bp    DNA     circular     10-JUN-2016
DEFINITION  pT2/SVNeo.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|715024180|
COMMENT     VNTDBDATE|-11275992|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pT2/SV_Neo-103|
COMMENT     VNTAUTHORNAME|KaaG|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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COMMENT                                   11.0089 12.1963 0 -10 3.172 1.04901 #249#)) 
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COMMENT                                   "@N" 1 -17.4506 -3.48466 0 -10 5.22007 
COMMENT                                   1.04901 #258#) 
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COMMENT                                   0 -10 8.74174 1.04901 #260#) 
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COMMENT                      #266=(CGroupWidget 
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COMMENT                                   "@N" 1 -8.98833 -13.9386 0 -10 5.12016 
COMMENT                                   1.04901 #267#) 
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COMMENT                                    (LOGPEN 0 0 3355545) 1 
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COMMENT               #274=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 875 100) 
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FEATURES             Location/Qualifiers
     misc_feature    34..56
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     promoter        49..65
                     /vntifkey="30"
                     /label=M13_forward20_primer
     promoter        75..93
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(4835..4855)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(4841..5056)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        4853..5121
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      5020..5097
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    5082..5101
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             5236..6030
                     /vntifkey="4"
                     /label=ORF\frame\1
     gene            5239..6027
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(5545..6081)
                     /vntifkey="4"
                     /label=ORF\frame\3
     terminator      6207..6338
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    6295..6314
                     /vntifkey="21"
                     /label=EBV_rev_primer
     promoter        complement(6760..6779)
                     /vntifkey="30"
                     /label=T3_promoter
     promoter        complement(6796..6814)
                     /vntifkey="30"
                     /label=M13_reverse_primer
     misc_feature    complement(6813..6835)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(6849..6878)
                     /vntifkey="30"
                     /label=lac_promoter
     gene            complement(7961..8821)
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             complement(7961..8821)
                     /vntifkey="4"
                     /label=ORF\frame\2
     promoter        complement(8863..8891)
                     /vntifkey="30"
                     /label=AmpR_promoter
     rep_origin      8970..9276
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    681..2873
                     /vntifkey="21"
                     /label=BphP1
     misc_feature    2916..3623
                     /vntifkey="21"
                     /label=mCherry
     misc_feature    3696..4319
                     /vntifkey="21"
                     /label=TetR
     promoter        482..602
                     /vntifkey="29"
                     /label=CMVd1
BASE COUNT     2166 a      2533 c      2571 g      2230 t 
ORIGIN
        1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
       61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca 
      121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta 
      181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt 
      241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat 
      301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt 
      361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagcttcc cacaataagt 
      421 tgggtgaatt ttggctcatt cctcctttct ataggattga ggtcagagct ttgtgatggc 
      481 tccaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg 
      541 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga 
      601 tcactagaag cttaatacga ctcactatag ggtacgattt aggtgacact atagaataag 
      661 gagcgatcgc catggctagc gtggcaggtc atgcctctgg cagccccgca ttcgggaccg 
      721 ccgatctttc gaattgcgaa cgtgaagaga tccacctcgc cggctcgatc cagccgcatg 
      781 gcgcgcttct ggtcgtcagc gagccggatc atcgcatcat ccaggccagc gccaacgccg 
      841 cggaatttct gaatctcgga agcgtgctcg gcgttccgct cgccgagatc gacggcgatc 
      901 tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg gtcgcggtgc 
      961 gctgccggat cggcaatccc tccacggagt acgacggtct gatgcatcgg cctccggaag 
     1021 gcgggctgat catcgagctc gaacgtgccg gcccgccgat cgatctgtcc ggcacgctgg 
     1081 cgccggcgct ggagcggatc cgcacggcgg gctcgctgcg cgcgctgtgc gatgacaccg 
     1141 cgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgc ttcgacgagc 
     1201 agggccacgg cgaagtgttc tccgagcgcc acgtgcccgg gctcgaatcc tatttcggca 
     1261 accgctatcc gtcgtcggac attccgcaga tggcgcggcg gctgtacgag cggcagcgcg 
     1321 tccgcgtgct ggtcgacgtc agctatcagc cggtgccgct ggagccgcgg ctgtcgccgc 
     1381 tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg ccgatccatc 
     1441 tgcagtacct gaagaacatg ggcgtgcgcg ccaccctggt ggtgtcgctg gtggtcggcg 
     1501 gcaagctgtg gggcctggtt gcctgtcatc attatctgcc gcgcttcatg catttcgagc 
     1561 tgcgggcgat ctgcgaactg ctcgccgaag cgatcgcgac gcggatcacc gcgcttgaga 
     1621 gcttcgcgca gagccagtcg gagctgttcg tgcagcggct cgaacagcgc atgatcgaag 
     1681 cgattacccg tgaaggcgat tggcgcgcag cgattttcga caccagccaa tcgatcctgc 
     1741 agccgctgca cgccgccggt tgcgcgctgg tgtacgaaga ccagatcagg accatcggcg 
     1801 acgtgccttc cacgcaggat gtgcgcgaga tcgccgggtg gctcgatcgc cagccgcgcg 
     1861 cggcggtgac ctcgaccgcg tcgctcggtc tcgacgtgcc ggagctcgcg catctgacgc 
     1921 ggatggcgag cggcgtggtc gcggcgccga tttcggatca tcgcggcgag tttctgatgt 
     1981 ggttccgccc cgagcgcgtc cacaccgtta cctggggcgg cgatccgaag aagccgttca 
     2041 cgatgggcga tacaccggcg gatctgtcgc cgcggcgctc cttcgccaaa tggcatcagg 
     2101 ttgtcgaagg cacgtccgat ccgtggacgg ccgccgatct cgccgcggct cgcaccatcg 
     2161 gtcagaccgt cgccgacatc gtgctgcaat tccgcgcggt gcggacactg atcgcccgcg 
     2221 aacagtacga acagttttcg tcccaggtgc acgcttcgat gcagccggtg ctgatcaccg 
     2281 acgccgaagg ccgcatcctg ctgatgaacg actcgttccg cgacatgttg ccggcgggtt 
     2341 cgccatccgc cgtccatctc gacgatctcg ccgggttctt cgtcgaatcg aacgatttcc 
     2401 tgcgcaacgt cgccgaactg atcgatcacg gccgcgggtg gcgcggcgaa gttctgctgc 
     2461 gcggcgcagg caaccgcccg ttgccgctgg cagtgcgcgc cgatccggtg acgcgcacgg 
     2521 aggaccagtc gctcggcttc gtgctgatct tcagcgacgc taccgatcgt cgcaccgcag 
     2581 atgccgcacg cacgcgtttc caggaaggca ttcttgccag cgcacgtccc ggcgtgcggc 
     2641 tcgactccaa gtccgacctg ttgcacgaga agctgctgtc cgcgctggtc gagaacgcgc 
     2701 agcttgccgc attggaaatc acttacggcg tcgagaccgg acgcatcgcc gagctgctcg 
     2761 aaggcgtccg ccagtcgatg ctgcgcaccg ccgaagtgct cggccatctg gtgcagcacg 
     2821 cggcgcgcac ggccggcagc gacagctcga gcaatggctc gcagaacaag aaggaattcg 
     2881 atagtgctgg tagtgctggt agtgctggta ctagtatggt gagcaagggc gaggaggata 
     2941 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg 
     3001 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 
     3061 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc 
     3121 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga 
     3181 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 
     3241 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc 
     3301 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg 
     3361 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga 
     3421 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca 
     3481 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc 
     3541 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg 
     3601 gcggcatgga cgagctgtac aagagtgctg gtgggtccgc cggcgggagt gctggcggta 
     3661 gtgccggcgg tagttctaga agtgctggtg ggtccatgac tagattagat aaaagtaaag 
     3721 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 
     3781 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 
     3841 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 
     3901 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 
     3961 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 
     4021 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 
     4081 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 
     4141 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 
     4201 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 
     4261 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcctaag 
     4321 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga 
     4381 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 
     4441 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 
     4501 cagggggaga tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc 
     4561 gaattctaat ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg 
     4621 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt 
     4681 tagcagccct tgcgccctga gtgcttgcgg cagcgtgagc ttcaaaagaa ttgccagctg 
     4741 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc 
     4801 caaggatctg atggcgcagg ggatcaaatt ctgtggaatg tgtgtcagtt agggtgtgga 
     4861 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     4921 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 
     4981 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 
     5041 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
     5101 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 
     5161 ttttgcaaaa agctcccggg agcttggata tccattttcg gatctgatca agagacagga 
     5221 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 
     5281 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 
     5341 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 
     5401 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 
     5461 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 
     5521 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 
     5581 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 
     5641 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 
     5701 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 
     5761 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 
     5821 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 
     5881 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 
     5941 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 
     6001 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 
     6061 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 
     6121 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 
     6181 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 
     6241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     6301 tgtccaaact catcaatgta tcttatcatg tctggatccc ttgaaataca tccacaggta 
     6361 cacctccaat tgactcaaat gatgtcaatt agtctatcag aagcttgtgg aaggctactc 
     6421 gaaatgtttg acccaagtta aacaatttaa aggcaatgct accaaatact aattgagtgt 
     6481 atgtaaactt ctgacccact gggaatgtga tgaaagaaat aaaagctgaa atgaatcatt 
     6541 ctctctacta ttattctgat atttcacatt cttaaaataa agtggtgatc ctaactgacc 
     6601 taagacaggg aatttttact aggattaaat gtcaggaatt gtgaaaaagt gagtttaaat 
     6661 gtatttggct aaggtgtatg taaacttccg acttcaactg tatagggatc ctctagctag 
     6721 agtcgacctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg 
     6781 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 
     6841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 
     6901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     6961 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     7021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     7081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     7141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     7201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     7261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     7321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     7381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     7441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     7501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     7561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     7621 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     7681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     7741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     7801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     7861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     7921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     7981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     8041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     8101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     8161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     8221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     8281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     8341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     8401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     8461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     8521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     8581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     8641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     8701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     8761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     8821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     8881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     8941 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     9001 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     9061 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 
     9121 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
     9181 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     9241 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 
     9301 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 
     9361 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt 
     9421 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 
     9481 ggcgaaaggg ggatgtgctg 
//
