LOCUS       pTRE-Tight-Rluc8        3841 bp    DNA     circular     10-JUN-2016
DEFINITION  Complementary copy of pTREtight2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11276051|
COMMENT     VNTDBDATE|-11276052|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTRE-Tight-Rluc8|
COMMENT     VNTAUTHORNAME|KaaG|
COMMENT     VNTOAUTHORNAME|Anton Shemetov|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Complementary copy of pTREtight2." 1) 
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COMMENT                          "Currently local object. Original author: Anton Shemetov" 
COMMENT                          1) 
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FEATURES             Location/Qualifiers
     misc_feature    9..258
                     /vntifkey="21"
                     /label=TREmod
     promoter        1799..1827
                     /vntifkey="30"
                     /label=AmpR_promoter
     CDS             1869..2729
                     /vntifkey="4"
                     /label=AmpR
     gene            1869..2729
                     /gene="Ampicillin"
                     /vntifkey="60"
     misc_feature    295..314
                     /vntifkey="21"
                     /label=pCEP_fwd_primer
     misc_feature    297..321
                     /vntifkey="21"
                     /label=LNCX_primer
     misc_feature    1618..1640
                     /vntifkey="21"
                     /label=pGEX_3_primer
     source          complement(1669..1668)
                     /organism="pTREtight2"
                     /mol_type="other DNA"
                     /vntifkey="98"
     CDS             359..1288
                     /vntifkey="4"
                     /label=Rluc8\(Nano-lantern)
     rep_origin      2877..3544
                     /vntifkey="33"
                     /label=pUC\ori
BASE COUNT      973 a       971 c      1013 g       884 t 
ORIGIN
        1 gtgtggctcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat 
       61 cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt 
      121 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa 
      181 cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca 
      241 gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct 
      301 cgtttagtga accgtcagat cgcctggaga attcgagctc ggtacccggg gatccaccat 
      361 gaaggtgtac gaccccgagc agaggaagag gatgatcacc ggcccccagt ggtgggccag 
      421 gtgcaagcag atgaacgtgc tggacagctt catcaactac tacgacagcg agaagcacgc 
      481 cgagaacgcc gtgatcttcc tgcacggcaa cgccactagc agctacctgt ggaggcacgt 
      541 ggtgccccac atcgagcccg tggccaggtg catcatcccc gatctgatcg gcatgggcaa 
      601 gagcggcaag agcggcaacg gcagctacag gctgctggac cactacaagt acctgaccgc 
      661 ctggttcgag cttctgaacc tgcccaagaa gatcatcttc gtgggccacg actggggcgc 
      721 cgccctggcc ttccactacg cctacgagca ccaggacagg atcaaggcca tcgtgcacat 
      781 ggagagcgtg gtggacgtga tcgagagctg ggacgagtgg ccagacatcg aggaggacat 
      841 cgccctgatc aagagcgagg agggcgagaa gatggtgctg gagaacaact tcttcgtgga 
      901 gaccgtgctg cccagcaaga tcatgagaaa gctggagccc gaggagttcg ccgcctacct 
      961 ggagcccttc aaggagaagg gcgaggtgag aagacccacc ctgagctggc ccagagagat 
     1021 ccccctggtg aagggcggca agcccgacgt ggtgcagatc gtgagaaact acaacgccta 
     1081 cctgagagcc agcgacgacc tgcccaagct gttcatcgag ggcgaccccg gcttcttcag 
     1141 caacgccatc gtggagggcg ccaagaagtt ccccaacacc gagttcgtga aggtgaaggg 
     1201 cctgcacttc ctccaggagg acgcccccga cgagatgggc aagtacatca agagcttcgt 
     1261 ggagagagtg ctgaagaacg agcagtaagc ggccgcatcg ataagcttgt cgacgatatc 
     1321 tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 
     1381 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 
     1441 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     1501 catttttttc actgcctcga gagttaagcc agccccgaca cccgccaaca cccgctgacg 
     1561 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
     1621 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 
     1681 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 
     1741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     1801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     1861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     1921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     1981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     2041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     2101 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     2161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     2221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     2281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     2341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     2401 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     2461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     2521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     2581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     2641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     2701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     2761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     2821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     2881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     2941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     3001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     3061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     3121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     3181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     3241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     3301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     3361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     3421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     3481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     3541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     3601 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     3661 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
     3721 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
     3781 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
     3841 t 
//
