LOCUS eSpCas9(1.1)_No_FLAG.xdna 8437 bp DNA circular 2016-07-07 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: PX330_-addgene_( - [ACCESSION]: u rn.local...1405433855830.449 - [SOURCE]: 1..8506 COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers promoter 1..249 /label=Human U6 Promoter /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=dnas_title="Human U6 Promoter" - vntifkey="29" - label=Human U6 Promoter - 1..249 \ misc_feature 268..343 /label=chimeric guide RNA scaffold /SerialCloner_Color=&hCC66FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="chimeric guide RNA scaffold" - 268..343 \ misc_feature 344..349 /label=U6 terminator /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="U6 terminator" - 344..349 \ misc_feature 440..1238 /label=CBh Promoter /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="CBh" - 440..1238 \ misc_feature 5460..5691 /label=bGH polyA /SerialCloner_Color=&hDC143C /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="bGH polyA" - 5529..5760 \ misc_feature 5700..5840 /label=R-ITR /SerialCloner_Color=&h666666 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=dnas_title="R-ITR" - vntifkey="21" - label=R-ITR - 5769..5909 \ rep_origin 5932..6238 /label=f1 Origin /SerialCloner_Color=&h666666 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=dnas_title="f1 Origin" - vntifkey="33" - label=f1 Origin - 6001..6307 \ misc_feature 6757..7614 /label=AmpR /SerialCloner_Color=&h666666 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=dnas_title="AmpR" - vntifkey="21" - label=AmpR - 6826..7683 \ rep_origin 7765..8432 /label=pUC ori /SerialCloner_Color=&h666666 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=dnas_title="pUC ori" - vntifkey="33" - label=pUC ori - 7834..8501 \ misc_feature 5403..5450 /label=Nucleoplasmin NLS /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 1302..5402 /label=eSpCas9 (1.1) ORF /SerialCloner_Color=&h0000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3840..3842 /label=K848A /SerialCloner_Color=&hFF0080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="A" \ misc_feature 4305..4307 /label=K1003A /SerialCloner_Color=&hFF0080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="A" \ misc_feature 4476..4478 /label=R1060A /SerialCloner_Color=&hFF0080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="A" \ misc_feature 252..257 /label=BbsI /SerialCloner_Color=&hFF0080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="VF" \ misc_feature 260..265 /label=BbsI /SerialCloner_Color=&hFF0080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="ED" \ misc_feature 1245..1254 /label=Kozak /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="ATMX" \ misc_feature 1257..1277 /label=SV40 NLS /SerialCloner_Color=&h00FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation" \ /SerialCloner_Desc=translation="PKKKRKV" \ ORIGIN 1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga 241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag 301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct 361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac 421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc 541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt 661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac 781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg 841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga 901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc 961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc 1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg 1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag 1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac 1201 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc ATGGCCCCAA 1261 AGAAGAAGCG GAAGGTCGGT ATCCACGGAG TCCCAGCAGC CGACAAGAAG TACAGCATCG 1321 GCCTGGACAT CGGCACCAAC TCTGTGGGCT GGGCCGTGAT CACCGACGAG TACAAGGTGC 1381 CCAGCAAGAA ATTCAAGGTG CTGGGCAACA CCGACCGGCA CAGCATCAAG AAGAACCTGA 1441 TCGGAGCCCT GCTGTTCGAC AGCGGCGAAA CAGCCGAGGC CACCCGGCTG AAGAGAACCG 1501 CCAGAAGAAG ATACACCAGA CGGAAGAACC GGATCTGCTA TCTGCAAGAG ATCTTCAGCA 1561 ACGAGATGGC CAAGGTGGAC GACAGCTTCT TCCACAGACT GGAAGAGTCC TTCCTGGTGG 1621 AAGAGGATAA GAAGCACGAG CGGCACCCCA TCTTCGGCAA CATCGTGGAC GAGGTGGCCT 1681 ACCACGAGAA GTACCCCACC ATCTACCACC TGAGAAAGAA ACTGGTGGAC AGCACCGACA 1741 AGGCCGACCT GCGGCTGATC TATCTGGCCC TGGCCCACAT GATCAAGTTC CGGGGCCACT 1801 TCCTGATCGA GGGCGACCTG AACCCCGACA ACAGCGACGT GGACAAGCTG TTCATCCAGC 1861 TGGTGCAGAC CTACAACCAG CTGTTCGAGG AAAACCCCAT CAACGCCAGC GGCGTGGACG 1921 CCAAGGCCAT CCTGTCTGCC AGACTGAGCA AGAGCAGACG GCTGGAAAAT CTGATCGCCC 1981 AGCTGCCCGG CGAGAAGAAG AATGGCCTGT TCGGAAACCT GATTGCCCTG AGCCTGGGCC 2041 TGACCCCCAA CTTCAAGAGC AACTTCGACC TGGCCGAGGA TGCCAAACTG CAGCTGAGCA 2101 AGGACACCTA CGACGACGAC CTGGACAACC TGCTGGCCCA GATCGGCGAC CAGTACGCCG 2161 ACCTGTTTCT GGCCGCCAAG AACCTGTCCG ACGCCATCCT GCTGAGCGAC ATCCTGAGAG 2221 TGAACACCGA GATCACCAAG GCCCCCCTGA GCGCCTCTAT GATCAAGAGA TACGACGAGC 2281 ACCACCAGGA CCTGACCCTG CTGAAAGCTC TCGTGCGGCA GCAGCTGCCT GAGAAGTACA 2341 AAGAGATTTT CTTCGACCAG AGCAAGAACG GCTACGCCGG CTACATTGAC GGCGGAGCCA 2401 GCCAGGAAGA GTTCTACAAG TTCATCAAGC CCATCCTGGA AAAGATGGAC GGCACCGAGG 2461 AACTGCTCGT GAAGCTGAAC AGAGAGGACC TGCTGCGGAA GCAGCGGACC TTCGACAACG 2521 GCAGCATCCC CCACCAGATC CACCTGGGAG AGCTGCACGC CATTCTGCGG CGGCAGGAAG 2581 ATTTTTACCC ATTCCTGAAG GACAACCGGG AAAAGATCGA GAAGATCCTG ACCTTCCGCA 2641 TCCCCTACTA CGTGGGCCCT CTGGCCAGGG GAAACAGCAG ATTCGCCTGG ATGACCAGAA 2701 AGAGCGAGGA AACCATCACC CCCTGGAACT TCGAGGAAGT GGTGGACAAG GGCGCTTCCG 2761 CCCAGAGCTT CATCGAGCGG ATGACCAACT TCGATAAGAA CCTGCCCAAC GAGAAGGTGC 2821 TGCCCAAGCA CAGCCTGCTG TACGAGTACT TCACCGTGTA TAACGAGCTG ACCAAAGTGA 2881 AATACGTGAC CGAGGGAATG AGAAAGCCCG CCTTCCTGAG CGGCGAGCAG AAAAAGGCCA 2941 TCGTGGACCT GCTGTTCAAG ACCAACCGGA AAGTGACCGT GAAGCAGCTG AAAGAGGACT 3001 ACTTCAAGAA AATCGAGTGC TTCGACTCCG TGGAAATCTC CGGCGTGGAA GATCGGTTCA 3061 ACGCCTCCCT GGGCACATAC CACGATCTGC TGAAAATTAT CAAGGACAAG GACTTCCTGG 3121 ACAATGAGGA AAACGAGGAC ATTCTGGAAG ATATCGTGCT GACCCTGACA CTGTTTGAGG 3181 ACAGAGAGAT GATCGAGGAA CGGCTGAAAA CCTATGCCCA CCTGTTCGAC GACAAAGTGA 3241 TGAAGCAGCT GAAGCGGCGG AGATACACCG GCTGGGGCAG GCTGAGCCGG AAGCTGATCA 3301 ACGGCATCCG GGACAAGCAG TCCGGCAAGA CAATCCTGGA TTTCCTGAAG TCCGACGGCT 3361 TCGCCAACAG AAACTTCATG CAGCTGATCC ACGACGACAG CCTGACCTTT AAAGAGGACA 3421 TCCAGAAAGC CCAGGTGTCC GGCCAGGGCG ATAGCCTGCA CGAGCACATT GCCAATCTGG 3481 CCGGCAGCCC CGCCATTAAG AAGGGCATCC TGCAGACAGT GAAGGTGGTG GACGAGCTCG 3541 TGAAAGTGAT GGGCCGGCAC AAGCCCGAGA ACATCGTGAT CGAAATGGCC AGAGAGAACC 3601 AGACCACCCA GAAGGGACAG AAGAACAGCC GCGAGAGAAT GAAGCGGATC GAAGAGGGCA 3661 TCAAAGAGCT GGGCAGCCAG ATCCTGAAAG AACACCCCGT GGAAAACACC CAGCTGCAGA 3721 ACGAGAAGCT GTACCTGTAC TACCTGCAGA ATGGGCGGGA TATGTACGTG GACCAGGAAC 3781 TGGACATCAA CCGGCTGTCC GACTACGATG TGGACCATAT CGTGCCTCAG AGCTTTCTGG 3841 CCGACGACTC CATCGACAAC AAGGTGCTGA CCAGAAGCGA CAAGAACCGG GGCAAGAGCG 3901 ACAACGTGCC CTCCGAAGAG GTCGTGAAGA AGATGAAGAA CTACTGGCGG CAGCTGCTGA 3961 ACGCCAAGCT GATTACCCAG AGAAAGTTCG ACAATCTGAC CAAGGCCGAG AGAGGCGGCC 4021 TGAGCGAACT GGATAAGGCC GGCTTCATCA AGAGACAGCT GGTGGAAACC CGGCAGATCA 4081 CAAAGCACGT GGCACAGATC CTGGACTCCC GGATGAACAC TAAGTACGAC GAGAATGACA 4141 AGCTGATCCG GGAAGTGAAA GTGATCACCC TGAAGTCCAA GCTGGTGTCC GATTTCCGGA 4201 AGGATTTCCA GTTTTACAAA GTGCGCGAGA TCAACAACTA CCACCACGCC CACGACGCCT 4261 ACCTGAACGC CGTCGTGGGA ACCGCCCTGA TCAAAAAGTA CCCTGCGCTG GAAAGCGAGT 4321 TCGTGTACGG CGACTACAAG GTGTACGACG TGCGGAAGAT GATCGCCAAG AGCGAGCAGG 4381 AAATCGGCAA GGCTACCGCC AAGTACTTCT TCTACAGCAA CATCATGAAC TTTTTCAAGA 4441 CCGAGATTAC CCTGGCCAAC GGCGAGATCC GGAAGGCGCC TCTGATCGAG ACAAACGGCG 4501 AAACCGGGGA GATCGTGTGG GATAAGGGCC GGGATTTTGC CACCGTGCGG AAAGTGCTGA 4561 GCATGCCCCA AGTGAATATC GTGAAAAAGA CCGAGGTGCA GACAGGCGGC TTCAGCAAAG 4621 AGTCTATCCT GCCCAAGAGG AACAGCGATA AGCTGATCGC CAGAAAGAAG GACTGGGACC 4681 CTAAGAAGTA CGGCGGCTTC GACAGCCCCA CCGTGGCCTA TTCTGTGCTG GTGGTGGCCA 4741 AAGTGGAAAA GGGCAAGTCC AAGAAACTGA AGAGTGTGAA AGAGCTGCTG GGGATCACCA 4801 TCATGGAAAG AAGCAGCTTC GAGAAGAATC CCATCGACTT TCTGGAAGCC AAGGGCTACA 4861 AAGAAGTGAA AAAGGACCTG ATCATCAAGC TGCCTAAGTA CTCCCTGTTC GAGCTGGAAA 4921 ACGGCCGGAA GAGAATGCTG GCCTCTGCCG GCGAACTGCA GAAGGGAAAC GAACTGGCCC 4981 TGCCCTCCAA ATATGTGAAC TTCCTGTACC TGGCCAGCCA CTATGAGAAG CTGAAGGGCT 5041 CCCCCGAGGA TAATGAGCAG AAACAGCTGT TTGTGGAACA GCACAAGCAC TACCTGGACG 5101 AGATCATCGA GCAGATCAGC GAGTTCTCCA AGAGAGTGAT CCTGGCCGAC GCTAATCTGG 5161 ACAAAGTGCT GTCCGCCTAC AACAAGCACC GGGATAAGCC CATCAGAGAG CAGGCCGAGA 5221 ATATCATCCA CCTGTTTACC CTGACCAATC TGGGAGCCCC TGCCGCCTTC AAGTACTTTG 5281 ACACCACCAT CGACCGGAAG AGGTACACCA GCACCAAAGA GGTGCTGGAC GCCACCCTGA 5341 TCCACCAGAG CATCACCGGC CTGTACGAGA CACGGATCGA CCTGTCTCAG CTGGGAGGCG 5401 ACAAAAGGCC GGCGGCCACG AAAAAGGCCG GCCAGGCAAA AAAGAAAAAG TAAgaattcc 5461 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 5521 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 5581 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 5641 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca 5701 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 5761 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg 5821 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg 5881 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc 5941 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 6001 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 6061 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 6121 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 6181 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 6241 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 6301 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 6361 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 6421 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 6481 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 6541 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 6601 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 6661 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 6721 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 6781 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 6841 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 6901 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 6961 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 7021 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 7081 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 7141 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 7201 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 7261 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 7321 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 7381 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 7441 attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg 7501 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 7561 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 7621 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 7681 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 7741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 7801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 7861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 7921 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 7981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 8041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 8101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 8161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 8221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 8281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 8341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 8401 cggttcctgg ccttttgctg gccttttgct cacatgt //