LOCUS       FancF_N_FS_v2_Donor    3542 bp      DNA     circular   2016-06-14
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : FancF_N_FS_v2_Donor_Step1 [3538  nt] : (EcoRI[
            2974] / EcoRI[1482])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    complement(2485..3342)
                     /label=AP(R)
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= bla gene- Ap(r) determinant  \ 
     misc_feature    1723..1723
                     /label=ORI
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= RNaseH cleavage point  \ 
     misc_feature    complement(2485..3342)
                     /label=AmpR
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1723..2310
                     /label=Ori
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3343..3407
                     /label=bla promoter
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    586..651
                     /label=3X FLAG
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    637..651
                     /label=Enterokinase
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    661..750
                     /label=Twin_Strep_Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    667..690
                     /label=Strep Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    727..750
                     /label=Strep Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    691..714
                     /label=(GGGS)2
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(1540..1559)
                     /label=pUC19 R
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    580..585
                     /label=NgoMIV
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    579..586
                     /label=SgrAI
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    194..564
                     /label=FancF Left Arm
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    565..574
                     /label=Kosak
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    757..1487
                     /label=FancF Right Arm
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatGGT ACCAACCTCA ACACAGATTC TAGTTTTAGG CAAAGCTCAG AAAATTTCTA 
      241 CTTAAGGATA TTTCCAAAGC GAAAGGAAGC GCGGAGACGT TCATGACTGG CATCATCTCG 
      301 CACGTGGTTC CGGAAATTCT CGGTAGGATG CCCTACATCT GCTCTCCCTC CACTAAGAAG 
      361 AACCTCTTTG TGTGGCGAAA GTAAAAGTAT TAGGGCTTTT AAGTTGCCCA GAGTCAAGGA 
      421 ACACGGATAA AGACGCTGGG AGATTGACAT GCATTTCGAC CAATAGCATT GCAGAGAGGC 
      481 GTATCATTTC GCGGATGTTC CAATCAGTAC GCAGAGAGTC GCCGTCTCCA AGGTGAAAGC 
      541 GGAAGTAGGG CCTTCGCGCA CCTCgccacc atgggatccg ccggcgacta caaggaccac 
      601 gacggcgatt ataaggatca cgacatcgac tacaaagacg acgatgacaa gggcgccagc 
      661 agcgcctggt cccaccctca gtttgagaag ggcggaggct ctggcggcgg aagcggagga 
      721 tctgcttgga gccaccccca gttcgaaaag ggcgccgaat cccttctgca gcacctggat 
      781 cgcttttccg agcttctggc ggtctcaagc actacctacg tcagcacctg ggaccccgcc 
      841 accgtgcgcc gggccttgca gtgggcgcgc tacctgcgcc acatccatcg gcgctttggt 
      901 cggcatggcc ccattcgcac ggctctggag cggcggctgc acaaccagtg gaggcaagag 
      961 ggcggctttg ggcggggtcc agttccggga ttagcgaact tccaggccct cggtcactgt 
     1021 gacgtcctgc tctctctgcg cctgctggag aaccgggccc tcggggatgc agctcgttac 
     1081 cacctggtgc agcaactctt tcccggcccg ggcgtccggg acgccgatga ggagacactc 
     1141 caagagagcc tggcccgcct tgcccgccgg cggtctgcgg tgcacatgct gcgcttcaat 
     1201 ggctatagag agaacccaaa tctccaggag gactctctga tgaagaccca ggcggagctg 
     1261 ctgctggagc gtctgcagga ggtggggaag gccgaagcgg agcgtcccgc caggtttctc 
     1321 agcagcctgt gggagcgctt gcctcagaac aacttcctga aggtgatagc ggtggcgctg 
     1381 ttgcagccgc ctttgtctcg tcggccccaa gaagagttgg aacccggcat ccacaaatca 
     1441 cctggagagg ggagccaagt gctagtccac tggcttctgg ggaattctgc attaatgaat 
     1501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     1561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     1621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     1681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     1741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     1801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     1861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     1921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     1981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     2041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     2101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     2161 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     2221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     2281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     2341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     2401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     2461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     2521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     2581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     2641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     2701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     2761 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     2821 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     2881 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     2941 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     3001 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     3061 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     3121 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     3181 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     3241 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     3301 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     3361 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     3421 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 
     3481 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 
     3541 tc
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