LOCUS       EZH2-TALEN_Right_No_FLAG    8029 bp      DNA     circular   2016-06-14
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : JDS78-36776 [7935  nt] : (Acc65I[1014] / NheI[
            895]) to pVAX-N2A-NLS-15556-FokELD [86  nt] : (NheI[696] / A
            cc65I[786])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    3095..3283
                     /label=C-terminal of repeat region
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=note="direct strand translation"translation="SIVAQLSRPDPALAALTNDHLVALACLGGRPALDAVKKGLPHAPALIKRTNRRIPERTSHRVA" \ 
     misc_feature    995..1399
                     /label=N-terminal of repeat region
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=note="direct strand translation"translation="VDLRTLGYSQQQQEKIKPKVRSTVAQHHEALVGHGFTHAHIVALSQHPAALGTVAVKYQDMIAALPEATHEAIVGVGKQWSGARALEALLTVAGELRGPPLQLDTGQLLKIAKRGGVTAVEAVHAWRNALTGAPL" \ 
     misc_feature    1400..3037
                     /label=TAL2362
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="NLTPDQVVAIANNNGGKQALETVQRLLPVLCQDHGLTPEQVVAIASHDGGKQALETVQRLLPVLCQAHGLTPDQVVAIASNGGGKQALETVQRLLPVLCQAHGLTPAQVVAIASNGGGKQALETVQRLLPVLCQDHGLTPDQVVAIASNGGGKQALETVQRLLPVLCQDHGLTPEQVVAIASNGGGKQALETVQRLLPVLCQAHGLTPD \ 
     misc_feature    3038..3094
                     /label=Half repeat
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=note="direct strand translation"translation="TPEQVVAIASNGGGRPALE" \ 
     polyA_signal    3990..4214
                     /label=BGH pA
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=2156..2380 \ 
     misc_feature    947..949
                     /label=ATG
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    953..973
                     /label=SV40 NLS
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 
       61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 
      121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 
      181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 
      241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 
      301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 
      361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 
      421 ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 
      481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 
      541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 
      601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 
      661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 
      721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 
      781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 
      841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGctagc 
      901 gtttaaactt aagctgatcc actagtccag tgtggtggaa ttcgccatgg cccccaagaa 
      961 gaagaggaag gtgggcatcc acggggtacC TATGGTGGAC TTGAGGACAC TCGGTTATTC 
     1021 GCAACAGCAA CAGGAGAAAA TCAAGCCTAA GGTCAGGAGC ACCGTCGCGC AACACCACGA 
     1081 GGCGCTTGTG GGGCATGGCT TCACTCATGC GCATATTGTC GCGCTTTCAC AGCACCCTGC 
     1141 GGCGCTTGGG ACGGTGGCTG TCAAATACCA AGATATGATT GCGGCCCTGC CCGAAGCCAC 
     1201 GCACGAGGCA ATTGTAGGGG TCGGTAAACA GTGGTCGGGA GCGCGAGCAC TTGAGGCGCT 
     1261 GCTGACTGTG GCGGGTGAGC TTAGGGGGCC TCCGCTCCAG CTCGACACCG GGCAGCTGCT 
     1321 GAAGATCGCG AAGAGAGGGG GAGTAACAGC GGTAGAGGCA GTGCACGCCT GGCGCAATGC 
     1381 GCTCACCGGG GCCCCCTTGA ACCTGACCCC AGACCAGGTA GTCGCAATCG CGAACAATAA 
     1441 TGGGGGAAAG CAAGCCCTGG AAACCGTGCA AAGGTTGTTG CCGGTCCTTT GTCAAGACCA 
     1501 CGGCCTTACA CCGGAGCAAG TCGTGGCCAT TGCAAATAAT AACGGTGGCA AACAGGCTCT 
     1561 TGAGACGGTT CAGAGACTTC TCCCAGTTCT CTGTCAAGCC CACGGGCTGA CTCCCGATCA 
     1621 AGTTGTAGCG ATTGCGTCCA ACGGTGGAGG GAAACAAGCA TTGGAGACTG TCCAACGGCT 
     1681 CCTTCCCGTG TTGTGTCAAG CCCACGGTTT GACGCCTGCA CAAGTGGTCG CCATCGCCAG 
     1741 CCATGATGGC GGTAAGCAGG CGCTGGAAAC AGTACAGCGC CTGCTGCCTG TACTGTGCCA 
     1801 GGATCATGGA CTGACCCCAG ACCAGGTAGT CGCAATCGCG TCACATGACG GGGGAAAGCA 
     1861 AGCCCTGGAA ACCGTGCAAA GGTTGTTGCC GGTCCTTTGT CAAGACCACG GCCTTACACC 
     1921 GGAGCAAGTC GTGGCCATTG CATCCCACGA CGGTGGCAAA CAGGCTCTTG AGACGGTTCA 
     1981 GAGACTTCTC CCAGTTCTCT GTCAAGCCCA CGGGCTGACT CCCGATCAAG TTGTAGCGAT 
     2041 TGCGTCCAAC GGTGGAGGGA AACAAGCATT GGAGACTGTC CAACGGCTCC TTCCCGTGTT 
     2101 GTGTCAAGCC CACGGTTTGA CGCCTGCACA AGTGGTCGCC ATCGCCTCGA ATGGCGGCGG 
     2161 TAAGCAGGCG CTGGAAACAG TACAGCGCCT GCTGCCTGTA CTGTGCCAGG ATCATGGACT 
     2221 GACCCCAGAC CAGGTAGTCG CAATCGCGTC ACATGACGGG GGAAAGCAAG CCCTGGAAAC 
     2281 CGTGCAAAGG TTGTTGCCGG TCCTTTGTCA AGACCACGGC CTTACACCGG AGCAAGTCGT 
     2341 GGCCATTGCA AGCAATGGGG GTGGCAAACA GGCTCTTGAG ACGGTTCAGA GACTTCTCCC 
     2401 AGTTCTCTGT CAAGCCCACG GGCTGACTCC CGATCAAGTT GTAGCGATTG CGTCGCATGA 
     2461 CGGAGGGAAA CAAGCATTGG AGACTGTCCA ACGGCTCCTT CCCGTGTTGT GTCAAGCCCA 
     2521 CGGTTTGACG CCTGCACAAG TGGTCGCCAT CGCCTCCAAT ATTGGCGGTA AGCAGGCGCT 
     2581 GGAAACAGTA CAGCGCCTGC TGCCTGTACT GTGCCAGGAT CATGGACTGA CCCCAGACCA 
     2641 GGTAGTCGCA ATCGCGAACA ATAATGGGGG AAAGCAAGCC CTGGAAACCG TGCAAAGGTT 
     2701 GTTGCCGGTC CTTTGTCAAG ACCACGGCCT TACACCGGAG CAAGTCGTGG CCATTGCAAG 
     2761 CAACATCGGT GGCAAACAGG CTCTTGAGAC GGTTCAGAGA CTTCTCCCAG TTCTCTGTCA 
     2821 AGCCCACGGG CTGACTCCCG ATCAAGTTGT AGCGATTGCG TCCAACGGTG GAGGGAAACA 
     2881 AGCATTGGAG ACTGTCCAAC GGCTCCTTCC CGTGTTGTGT CAAGCCCACG GTTTGACGCC 
     2941 TGCACAAGTG GTCGCCATCG CCTCGAATGG CGGCGGTAAG CAGGCGCTGG AAACAGTACA 
     3001 GCGCCTGCTG CCTGTACTGT GCCAGGATCA TGGACTGACA CCCGAACAGG TGGTCGCCAT 
     3061 TGCTTCTAAT GGGGGAGGAC GGCCAGCCTT GGAGTCCATC GTAGCCCAAT TGTCCAGGCC 
     3121 CGATCCCGCG TTGGCTGCGT TAACGAATGA CCATCTGGTG GCGTTGGCAT GTCTTGGTGG 
     3181 ACGACCCGCG CTCGATGCAG TCAAAAAGGG TCTGCCTCAT GCTCCCGCAT TGATCAAAAG 
     3241 AACCAACCGG CGGATTCCCG AGAGAACTTC CCATCGAGTC GCGGGATCCC AACTAGTCAA 
     3301 AAGTGAACTG GAGGAGAAGA AATCTGAACT TCGTCATAAA TTGAAATATG TGCCTCATGA 
     3361 ATATATTGAA TTAATTGAAA TTGCCAGAAA TTCCACTCAG GATAGAATTC TTGAAATGAA 
     3421 GGTAATGGAA TTTTTTATGA AAGTTTATGG ATATAGAGGT AAACATTTGG GTGGATCAAG 
     3481 GAAACCGGAC GGAGCAATTT ATACTGTCGG ATCTCCTATT GATTACGGTG TGATCGTGGA 
     3541 TACTAAAGCT TATAGCGGAG GTTATAATCT GCCAATTGGC CAAGCAGATG AAATGCAACG 
     3601 ATATGTCGAA GAAAATCAAA CACGAAACAA ACATATCAAC CCTAATGAAT GGTGGAAAGT 
     3661 CTATCCATCT TCTGTAACGG AATTTAAGTT TTTATTTGTG AGTGGTCACT TTAAAGGAAA 
     3721 CTACAAAGCT CAGCTTACAC GATTAAATCA TATCACTAAT TGTAATGGAG CTGTTCTTAG 
     3781 TGTAGAAGAG CTTTTAATTG GTGGAGAAAT GATTAAAGCC GGCACATTAA CCTTAGAGGA 
     3841 AGTCAGACGG AAATTTAATA ACGGCGAGAT AAACTTTTAA GGGCCCTTCG AAGGTAAGCC 
     3901 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC ACCATCACCA 
     3961 TTGAGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 
     4021 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 
     4081 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 
     4141 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC 
     4201 GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA 
     4261 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC 
     4321 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC 
     4381 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT TCCGATTTAG 
     4441 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC 
     4501 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG 
     4561 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 
     4621 AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 
     4681 CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4741 GGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC 
     4801 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT 
     4861 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC 
     4921 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA 
     4981 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC 
     5041 GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA ATCATCGGCA 
     5101 TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC CAAGCCTTTG 
     5161 TCTCAAGAAG AATCCACCCT CATTGAAAGA GCAACGGCTA CAATCAACAG CATCCCCATC 
     5221 TCTGAAGACT ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT CTTCACTGGT 
     5281 GTCAATGTAT ATCATTTTAC TGGGGGACCT TGTGCAGAAC TCGTGGTGCT GGGCACTGCT 
     5341 GCTGCTGCGG CAGCTGGCAA CCTGACTTGT ATCGTCGCGA TCGGAAATGA GAACAGGGGC 
     5401 ATCTTGAGCC CCTGCGGACG GTGTCGACAG GTGCTTCTCG ATCTGCATCC TGGGATCAAA 
     5461 GCGATAGTGA AGGACAGTGA TGGACAGCCG ACGGCAGTTG GGATTCGTGA ATTGCTGCCC 
     5521 TCTGGTTATG TGTGGGAGGG CTAAGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT 
     5581 ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG 
     5641 GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC 
     5701 CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 
     5761 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 
     5821 TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT 
     5881 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 
     5941 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 
     6001 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 
     6061 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 
     6121 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6181 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6241 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6301 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6361 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6421 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG 
     6481 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6541 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6601 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6661 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 
     6721 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6781 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6841 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6901 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6961 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 
     7021 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 
     7081 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 
     7141 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 
     7201 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 
     7261 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 
     7321 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 
     7381 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 
     7441 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 
     7501 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 
     7561 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 
     7621 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 
     7681 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 
     7741 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 
     7801 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 
     7861 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 
     7921 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 
     7981 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC
//