LOCUS       EZH2_N_FS_v2_Donor    3251 bp      DNA     circular   2016-06-14
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : Ligation #1 [2901  nt] : (EcoRI[4139] / #EciI[
            831]) to EZH2_genomic copy [347  nt] : (#EciI[466] / EcoRI[8
            29])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    complement(2194..3051)
                     /label=AP(R)
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= bla gene- Ap(r) determinant  \ 
     misc_feature    1432..1432
                     /label=ORI
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= RNaseH cleavage point  \ 
     misc_feature    complement(2194..3051)
                     /label=AmpR
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1432..2019
                     /label=Ori
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3052..3116
                     /label=bla promoter
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    617..682
                     /label=3X FLAG
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    668..682
                     /label=Enterokinase
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    692..781
                     /label=Twin_Strep_Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    698..721
                     /label=Strep Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    758..781
                     /label=Strep Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    722..745
                     /label=(GGGS)2
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(1249..1268)
                     /label=pUC19 R
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    611..616
                     /label=NgoMIV
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    610..617
                     /label=SgrAI
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    782..787
                     /label=GA linker
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    192..217
                     /label=LA
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    596..605
                     /label=Kosak
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1186..1196
                     /label=XhoI/EcoRI
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    183..193
                     /label=NdeI/Acc65I
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    192..595
                     /label=EZH2 HA-L
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    788..901
                     /label=Exon1 ORF (No ATG)
                     /SerialCloner_Color=&h996633
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    788..1185
                     /label=EZH2 HA-R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatGGT ACCATTATGC CTTGCTACTG TAACAGCTAA TGATGAAAAA CAGGATCTGT 
      241 CTTTATATTT TCTTCCCTCC ACAAATGTGG ATCTCATAGA GTTGAAAACT AGGTTGTGAT 
      301 ATAGTATAGT ATACCTAATT CCTGTAATGG GATCATGTTC CTATAATATG GCCGCAATTT 
      361 AGTGTAGAAT TTTTGTAAAT AAAAGTGTAT TTTAAGTTTA ACTTAAACTT TCAATGAAGT 
      421 GTTTTAAGGA TTTAACCATG CAGCACAAAT GAGCACCTTT CTGTAAATGC CAACAGTGTA 
      481 ATATGTGTCA TTTCTTCACT GATTGTTAGT TTGCTGCGGA TTAAAACACA GGTGATCATA 
      541 TTCAGGCTGG TTAGATTAGT GATTTTAATA TGAAACCATT GCTTTTAGaa taatcgccac 
      601 catgggatcc gccggcgact acaaggacca cgacggcgat tataaggatc acgacatcga 
      661 ctacaaagac gacgatgaca agggcgccag cagcgcctgg tcccaccctc agtttgagaa 
      721 gggcggaggc tctggcggcg gaagcggagg atctgcttgg agccaccccc agttcgaaaa 
      781 gGGCGCCGGC CAGACTGGGA AGAAATCTGA GAAGggacca gtttgttggc ggaagcgtgt 
      841 aaaatcagag tacatgcgac tgagacagct caagaggttc agacgagctg atgaagtaaa 
      901 gGTATAATTT TATCTTTTGT GAAAATGAAT ATACAATTAA GGATAGGTTT TTTCCCCTCC 
      961 TAAGTTCAAT AAGTTTTTAT TTAAAATAAT TCTAGCCAAG TTTGTATTTA CAATAAGATG 
     1021 AGTGAATACT TGGAAGCTTA GGTTCTTGAT CTGTCTTAAC ATTATACCAT GCACATTAAT 
     1081 CAGTCTTTGT TTAGTATACT TTTATTTGCA TATGCAGCTG CAGAAATGCC TCCCTAAATA 
     1141 TAAAGGGATG CATTTCATGA TTTTATGTTT CTAAAGTCAT TGTGActcga gaattctgca 
     1201 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     1261 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     1321 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     1381 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     1441 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     1501 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     1561 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     1621 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     1681 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     1741 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     1801 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     1861 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     1921 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     1981 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     2041 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     2101 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     2161 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     2221 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     2281 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     2341 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     2401 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     2461 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     2521 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     2581 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     2641 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     2701 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     2761 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     2821 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     2881 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     2941 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     3001 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     3061 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     3121 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     3181 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 
     3241 gccctttcgt c
//