LOCUS pWUKI-Cas1_V33A_ 6068 bp DNA circular UNA 20-JUN-2016 DEFINITION A new nucleotide sequence entered manually. ACCESSION urn.local...l1-4cdaw01 (modified) VERSION urn.local...l1-4cdaw01 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..19 /label="T7 prom" misc_feature 19..46 /note="Geneious type: regulatory sequence" /label="lacO reg" RBS 75..80 /label="RBS" gene 88..1005 /label="Cas1" misc_feature 185 /note="Geneious type: Editing History Replacement" /label="T" gene 1007..1291 /label="Cas2" terminator 1409..1456 /label="T7 terminator" misc_difference 1545..1666 /label="""""pLac""""" misc_feature 1593..1629 /label="Plac" misc_feature 1629..1649 /label="LacI operator" gene 1667..1969 /label="Smt3" misc_feature complement(1971..1998) /label="Repeat" promoter complement(1999..>2067) /label="Leader" gene 2080..2892 /label="Kanamycin resistance (no start)" gene complement(2984..3775) /label="SmR (aadA)" promoter complement(3817..3845) /label="amp prom" rep_origin complement(3915..4653) /label="pCDF Ori" gene complement(4863..5945) /label="lacI" promoter complement(5946..6023) /label="lacI promoter" ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt 61 tgtttaactt taataaggag atataccatg acctggcttc cccttaatcc cattccactc 121 aaagatcgcg tctccatgat ctttctgcaa tatgggcaga tcgatgtaat agatggcgcg 181 tttgcactta tcgacaagac agggatccgc actcatattc ctgttggctc ggttgcctgc 241 atcatgctgg aacctggtac acgggtttcg catgcagctg tacgcctggc tgcgcaagtt 301 ggaacattgt tggtatgggt gggggaagcg ggcgttcgtg tttatgcttc tggtcagcct 361 ggaggtgcgc gttcagataa gctgctctat caggcaaaac ttgctctgga tgaagatttg 421 cgtctgaagg tcgtacgtaa aatgtttgaa cttcggtttg gagaacctgc gcctgcccgg 481 cgctccgtag agcaactcag aggtatagaa ggcagtcgcg tgcgggcaac ctacgcactt 541 ctggcgaagc aatacggcgt gacatggaat ggacgtcgct acgatccgaa agactgggaa 601 aagggcgata cgatcaacca atgcattagc gctgcaactt cctgtttata cggcgtaact 661 gaagcggcga tacttgcagc tggttatgca ccagctattg ggtttgtgca tacaggaaag 721 cctctttcct ttgtttacga tattgcagac atcattaaat ttgacactgt tgtaccgaaa 781 gcttttgaga tagcgcgtcg taaccctggt gagccggacc gggaagtccg tttggcgtgc 841 agggatattt ttcgcagtag taaaacatta gccaaattga ttccgcttat agaggacgtg 901 cttgccgctg gagaaataca accgccggcc ccacctgaag atgcacagcc tgttgccatt 961 ccgcttcctg tttcactggg agatgcaggc catcggagta gctgaaatga gtatgttggt 1021 cgtggtcact gaaaatgtac ctccgcgctt acgaggcaga ttagccatct ggttgttgga 1081 ggtacgtgca ggggtatatg taggtgatgt atccgcaaaa attcgtgaaa tgatctggga 1141 acaaatagct ggactggcgg aagaaggcaa tgtagtgatg gcatgggcaa cgaatacgga 1201 aacgggattt gagttccaga catttgggtt aaacaggcgt accccggtag atttggatgg 1261 tttaaggttg gtgtcttttt tacctgtttg agcggccgca ctcgagtctg gtaaagaaac 1321 cgctgctgcg aaatttgaac gccagcacat ggactcgtct actagcgcag cttaattaac 1381 ctaggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt 1441 cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc 1501 ggtagtcaat aaaccggtaa accagcaata gacataagcg gtgagcgcaa cgcaattaat 1561 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 1621 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatgg ggtccctgca 1681 ggactcagaa gtcaatcaag aagctaagcc agaggtcaag ccagaagtca agcctgagac 1741 tcacatcaat ttaaaggtgt ccgatggatc ttcagagatc ttcttcaaga tcaaaaagac 1801 cactccttta agaaggctga tggaagcgtt cgctaaaaga cagggtaagg aaatggactc 1861 cttaagattc ttgtacgacg gtattagaat tcaagctgat caggcccctg aagatttgga 1921 catggaggat aacgatatta ttgaggctca ccgcgaacag attggaggtc ggtttatccc 1981 cgctggcgcg gggaactctc taaaagtata catttgttct taaagcattt tttcccatac 2041 aaacaaccca ccaaccttaa tgtaacagga tctggcagta gccatattca acgggaaacg 2101 tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 2161 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat 2221 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 2281 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 2341 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag 2401 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 2461 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgaccg cgtatttcgt 2521 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 2581 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 2641 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 2701 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 2761 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 2821 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 2881 gagtttttct aataaccgct atttaacgac cctgccctga accgacgacc gggtcatcgt 2941 ggccggatct tgcggcccct cggcttgaac gaattgttag acattatttg ccgactacct 3001 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca 3061 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact 3121 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg 3181 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc 3241 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt 3301 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc 3361 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg 3421 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc 3481 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct 3541 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat 3601 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat 3661 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga 3721 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatactct 3781 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 3841 ttgaatgtat ttagaaaaat aaacaaatag ctagctcact cggtcgctac gctccgggcg 3901 tgagactgcg gcgggcgctg cggacacata caaagttacc cacagattcc gtggataagc 3961 aggggactaa catgtgaggc aaaacagcag ggccgcgccg gtggcgtttt tccataggct 4021 ccgccctcct gccagagttc acataaacag acgcttttcc ggtgcatctg tgggagccgt 4081 gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac 4141 cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg tttaccggat 4201 acctgttccg cctttctccc ttacgggaag tgtggcgctt tctcatagct cacacactgg 4261 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga actccccgtt 4321 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc ggaaaagcac 4381 ggtaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc 4441 taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt 4501 tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa 4561 ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc 4621 agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgct ctagatttca 4681 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa 4741 ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca 4801 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc 4861 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 4921 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt 4981 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg 5041 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata 5101 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc 5161 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc 5221 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg 5281 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta 5341 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg 5401 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg 5461 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca 5521 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc 5581 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg 5641 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc 5701 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc 5761 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc 5821 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg 5881 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt 5941 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag 6001 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat 6061 taggaaat //