LOCUS pWUR_Cas1+2_tetO 7356 bp DNA circular UNA 14-JUN-2016 DEFINITION A new nucleotide sequence entered manually. ACCESSION urn.local...3a-68s1dj2 VERSION urn.local...3a-68s1dj2 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..19 /label="T7 prom" misc_feature 19..46 /note="Geneious type: regulatory sequence" /label="lacO reg" RBS 75..80 /label="RBS" gene 88..1005 /label="Cas1" gene 1007..1291 /label="Cas2" terminator 1300..1724 /label="rrnB terminator" promoter 1745..1835 /note="pLtetO from pZE21" /label="pLtetO from pZE21 promoter" RBS 1836..1861 /label="g10 RBS" gene 1862..2779 /label="Cas1" misc_feature 1933 /note="Geneious type: Editing History Replacement" /label="G" misc_feature 2252 /note="Geneious type: Editing History Replacement" /label="C" misc_feature 2401 /note="Geneious type: Editing History Replacement" /label="T" misc_feature 2466 /note="Geneious type: Editing History Replacement" /label="C" misc_feature 2583 /note="Geneious type: Editing History Replacement" /label="G" misc_feature 2689 /note="Geneious type: Editing History Replacement" /label="A" misc_feature 2751 /note="Geneious type: Editing History Replacement" /label="T" misc_feature 2764 /note="Geneious type: Editing History Replacement" /label="C" gene 2781..3065 /label="Cas2" terminator 3091..3132 /note="tetA/orfL bidirectional terminator termination of bla transcript is slightly weaker than opposite direction" /label="bidir terminator" promoter 3150..3293 /note="proA1 rrnB P1 Davis et al 2011" /label="proA1 rrnB P1 Davis et al 2011 promoter" RBS 3294..3313 /note="Strong RBS Hybrid Rinquest SD8 100 and Salis 235826" /label="RBS" CDS 3314..3937 /note="tet repressor" /label="tetR repressor" terminator 4054..4101 /label="T7 terminator" gene complement(4272..5063) /label="SmR (aadA)" promoter complement(5105..5133) /label="amp prom" rep_origin complement(5203..5941) /label="pCDF Ori" gene complement(6151..7233) /label="lacI" promoter complement(7234..7311) /label="lacI promoter" ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt 61 tgtttaactt taataaggag atataccatg acctggcttc cccttaatcc cattccactc 121 aaagatcgcg tctccatgat ctttctgcaa tatgggcaga tcgatgtaat agatggcgcg 181 tttgtactta tcgacaagac agggatccgc actcatattc ctgttggctc ggttgcctgc 241 atcatgctgg aacctggtac acgggtttcg catgcagctg tacgcctggc tgcgcaagtt 301 ggaacattgt tggtatgggt gggggaagcg ggcgttcgtg tttatgcttc tggtcagcct 361 ggaggtgcgc gttcagataa gctgctctat caggcaaaac ttgctctgga tgaagatttg 421 cgtctgaagg tcgtacgtaa aatgtttgaa cttcggtttg gagaacctgc gcctgcccgg 481 cgctccgtag agcaactcag aggtatagaa ggcagtcgcg tgcgggcaac ctacgcactt 541 ctggcgaagc aatacggcgt gacatggaat ggacgtcgct acgatccgaa agactgggaa 601 aagggcgata cgatcaacca atgcattagc gctgcaactt cctgtttata cggcgtaact 661 gaagcggcga tacttgcagc tggttatgca ccagctattg ggtttgtgca tacaggaaag 721 cctctttcct ttgtttacga tattgcagac atcattaaat ttgacactgt tgtaccgaaa 781 gcttttgaga tagcgcgtcg taaccctggt gagccggacc gggaagtccg tttggcgtgc 841 agggatattt ttcgcagtag taaaacatta gccaaattga ttccgcttat agaggacgtg 901 cttgccgctg gagaaataca accgccggcc ccacctgaag atgcacagcc tgttgccatt 961 ccgcttcctg tttcactggg agatgcaggc catcggagta gctgaaatga gtatgttggt 1021 cgtggtcact gaaaatgtac ctccgcgctt acgaggcaga ttagccatct ggttgttgga 1081 ggtacgtgca ggggtatatg taggtgatgt atccgcaaaa attcgtgaaa tgatctggga 1141 acaaatagct ggactggcgg aagaaggcaa tgtagtgatg gcatgggcaa cgaatacgga 1201 aacgggattt gagttccaga catttgggtt aaacaggcgt accccggtag atttggatgg 1261 tttaaggttg gtgtcttttt tacctgtttg agcggccgcg gctgttttgg cggatgagag 1321 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat 1381 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa 1441 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca 1501 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1561 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca 1621 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca 1681 gaaggccatc cgtcaggatg gcctttttgc gtttctacaa actctgctag caagtaaggc 1741 cgactcgagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag 1801 cacatcagca ggacgcactg accgaattca ttaaatttaa ctttaagaag gagatataca 1861 tatgacctgg cttcccctta atcccattcc actcaaagat cgcgtctcca tgatctttct 1921 gcaatatggg cacatcgatg taatagatgg cgcgtttgta cttatcgaca agacagggat 1981 ccgcactcat attcctgttg gctcggttgc ctgcatcatg ctggaacctg gtacacgggt 2041 ttcgcatgca gctgtacgcc tggctgcgca agttggaaca ttgttggtat gggtggggga 2101 agcgggcgtt cgtgtttatg cttctggtca gcctggaggt gcgcgttcag ataagctgct 2161 ctatcaggca aaacttgctc tggatgaaga tttgcgtctg aaggtcgtac gtaaaatgtt 2221 tgaacttcgg tttggagaac ctgcgcctgc caggcgctcc gtagagcaac tcagaggtat 2281 agaaggcagt cgcgtgcggg caacctacgc acttctggcg aagcaatacg gcgtgacatg 2341 gaatggacgt cgctacgatc cgaaagactg ggaaaagggc gatacgatca accaatgcat 2401 aagcgctgca acttcctgtt tatacggcgt aactgaagcg gcgatacttg cagctggtta 2461 tgcacaagct attgggtttg tgcatacagg aaagcctctt tcctttgttt acgatattgc 2521 agacatcatt aaatttgaca ctgttgtacc gaaagctttt gagatagcgc gtcgtaaccc 2581 tgatgagccg gaccgggaag tccgtttggc gtgcagggat atttttcgca gtagtaaaac 2641 attagccaaa ttgattccgc ttatagagga cgtgcttgcc gctggagata tacaaccgcc 2701 ggccccacct gaagatgcac agcctgttgc cattccgctt cctgtttcac agggagatgc 2761 aggtcatcgg agtagctgaa atgagtatgt tggtcgtggt cactgaaaat gtacctccgc 2821 gcttacgagg cagattagcc atctggttgt tggaggtacg tgcaggggta tatgtaggtg 2881 atgtatccgc aaaaattcgt gaaatgatct gggaacaaat agctggactg gcggaagaag 2941 gcaatgtagt gatggcatgg gcaacgaata cggaaacggg atttgagttc cagacatttg 3001 ggttaaacag gcgtaccccg gtagatttgg atggtttaag gttggtgtct tttttacctg 3061 tttgagcctt aattaacggc actcctcagc aaatataatg accctcttga taacccaaga 3121 gggcattttt taatgcccat ggcgtttacc acagctaaca ccacgtcgtc cctatctgct 3181 gccctaggtc tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtaggct 3241 atattcaggg agaccacaac ggtttccctc tacaaataat tttgtttaac tttgaaataa 3301 ggaggtaata caaatgtctc gtttagataa aagtaaagtg attaacagcg cattagagct 3361 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt 3421 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc 3481 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca 3541 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg 3601 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca 3661 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc 3721 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga 3781 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt 3841 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg 3901 attagaaaaa caacttaaat gtgaaagtgg gtcttaataa gcgacctcga gtctggtaaa 3961 gaaaccgctg ctgcgaaatt tgaacgccag cacatggact cgtctactag cgcagcttaa 4021 ttaacctagg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa 4081 cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg 4141 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc 4201 tgccctgaac cgacgaccgg gtcatcgtgg ccggatcttg cggcccctcg gcttgaacga 4261 attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat 4321 tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt 4381 ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca 4441 gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta 4501 agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt 4561 tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct 4621 ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg 4681 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat 4741 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg 4801 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg 4861 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca 4921 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc 4981 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact 5041 tcggcgatca ccgcttccct catactcttc ctttttcaat attattgaag catttatcag 5101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaatagct 5161 agctcactcg gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca 5221 aagttaccca cagattccgt ggataagcag gggactaaca tgtgaggcaa aacagcaggg 5281 ccgcgccggt ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac 5341 gcttttccgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg 5401 aaacccgaca ggacttaaag atccccaccg tttccggcgg gtcgctccct cttgcgctct 5461 cctgttccga ccctgccgtt taccggatac ctgttccgcc tttctccctt acgggaagtg 5521 tggcgctttc tcatagctca cacactggta tctcggctcg gtgtaggtcg ttcgctccaa 5581 gctgggctgt aagcaagaac tccccgttca gcccgactgc tgcgccttat ccggtaactg 5641 ttcacttgag tccaacccgg aaaagcacgg taaaacgcca ctggcagcag ccattggtaa 5701 ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca 5761 aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag ccagttacca 5821 cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt 5881 ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 5941 cttttctact gaaccgctct agatttcagt gcaatttatc tcttcaaatg tagcacctga 6001 agtcagcccc atacgatata agttgtaatt ctcatgttag tcatgccccg cgcccaccgg 6061 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga 6121 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 6181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 6241 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc 6301 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc 6361 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc 6421 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc 6481 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat 6541 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg 6601 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac 6661 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc 6721 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc 6781 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc 6841 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg 6901 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc 6961 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc 7021 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac 7081 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc 7141 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata 7201 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc 7261 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc 7321 gacgctctcc cttatgcgac tcctgcatta ggaaat //