LOCUS pBPGUw-QF2HACK 6582 bp ds-DNA circular SYN 04-Feb-2016 DEFINITION - ACCESSION - KEYWORDS - SOURCE - COMMENT Generated by Chun-Chieh Lin, Potter Lab, Johns Hopkins University School of Medicine FEATURES Location/Qualifiers misc_feature 50..77 /note=mFRT71 misc_feature complement(900..1760) /note=Amp misc_feature 1965..1992 /note=mFRT71 misc_feature 2008..2061 /note=T2A gene 2068..3120 /note=QF2 terminator 3128..3384 /note=hsp70 termination misc_feature 3394..3427 /note=LoxP misc_feature complement(3726..4436) /note=RFP promoter complement(4563..4613) /note=3xP3 misc_feature 4620..4653 /note=LoxP misc_feature 4676..5472 /note=U6:1 promoter misc_feature 5491..5570 /note=gRNA core misc_feature 5571..5987 /note=U6:3 promoter misc_feature 6006..6085 /note=gRNA core misc_feature complement(6543..6582) /note=attB40 BASE COUNT 1638 A 1669 C 1524 G 1751 T 0 OTHER ORIGIN ? 1 CACCGCGGCT TCGAGACCGT GACCTACATC GTCGACGGTA ACATGTGAAG TTTCTATACT 61 TTCTAGAGAA TAGAAACTTC CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 121 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 181 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 241 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 301 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 361 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 421 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 481 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 541 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 601 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 661 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 721 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 781 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 841 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 901 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 961 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 1021 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 1081 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 1141 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 1201 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 1261 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 1321 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 1381 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 1441 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 1501 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 1561 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 1621 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 1681 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 1741 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 1801 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 1861 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA 1921 TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTA CGAAGTTTCT ATACTTTCTA 1981 GAGAATAGAA ACTTCTACGT AACGCGTGAG GGCCGCGGCA GCCTGCTGAC CTGCGGCGAT 2041 GTGGAGGAGA ACCCCGGGCC CCCTAGGATG CCACCCAAGC GCAAAACGCT TAACGCTGCG 2101 GCTGAGGCTA ACGCTCATGC CGACGGACAC GCCGACGGAA ACGCCGACGG ACACGTGGCC 2161 AATACGGCCG CGTCCTCGAA TAATGCGAGG TTCGCTGATC TCACTAACAT CGATACTCCG 2221 GGTCTGGGAC CCACAACTAC GACCCTGCTC GTGGAACCAG CACGCTCAAA GCGTCAACGA 2281 GTGTCCCGCG CATGCGACCA GTGCCGTGCA GCCCGAGAGA AATGCGACGG AATACAGCCT 2341 GCGTGTTTCC CGTGCGTTTC CCAGGGAAGG TCCTGCACTT ATCAGGCTTC GCCGAAAAAG 2401 AGGGGAGTTC AAACCGGTTA TATTCGTACG CTGGAGCTCG CCCTCGCCTG GATGTTTGAA 2461 AATGTCGCGC GTTCCGAAGA TGCCTTGCAT AACCTCCTCG TCCGTGACGC CGGACAAGGA 2521 TCAGCTCTGC TCGTTGGTAA AGATTCGCCG GCTGCCGAGC GACTCCATGC CCGTTGGGCT 2581 ACTAGCCGTG TCAATAAGAG CATTACCCGC CTCCTCCGTC AGTTGGAGCT CCCTCCTACC 2641 GCCACGGCTA CGGCCTCGAT AATGCCGCAC GTGATGGAGC AGCCTCTCAG TACCAGCATT 2701 AACCCCGTCA ACGACCGCTT CAACGGTATT CCCAACCCCA CTCCGTATAA CTCCGATGCA 2761 GCTCTCGATG CTATCACTCA GACCAACGAT TATGGAAGCG TAAATACACA TGGTATCCTC 2821 TCTACTTACC CGCCACCGGC TACGCACCTT AATGAAGCTT CCGTCGCTCT CGCTCCCGGT 2881 GGCGCCCCCC CCCGACCGCC TCCTCCGTAT GTTGACAGCA CGACCAATCA CCCGCCGTAC 2941 CACTCGAATC TGGTTCCAAT GGCGAACTTT GGTTACTCGA CCGTTGATTA CGATGCCATG 3001 GTTGACGATT TGGCTAGCAT TGAATACACG GACGCTGTGG ATGTCGACCC ACAGTTTATG 3061 ACCAATCTGG GATTCGTTCC TGGATGTAAC TTCTCCGACA TTAATACATA CGAACAGTGA 3121 TGACGTCTAT CGATACCGTC GACTAAAGCC AAATAGAAAA TTATTCAGTT CCTGGCTTAA 3181 GTTTTTAAAA GTGATATTAT TTATTTGGTT GTAACCAACC AAAAGAATGT AAATAACTAA 3241 TACATAATTA TGTTAGTTTT AAGTTAGCAA CAAATTGATT TTAGCTATAT TAGCTACTTG 3301 GTTAATAAAT AGAATATATT TATTTAAAGA TAATTGCGTT TTTATTGTCA GGGAGTGAGT 3361 TTGCTTAAAA ACTCGTTTAG ATCCACCGCA TGCATAACTT CGTATAGCAT ACATTATACG 3421 AAGTTATGGT ACCACTCGAC GATTAACCGT TAGTTTAGCA AGTATAATTG AATTTTTTAT 3481 AAGATCCAGC TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA 3541 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC 3601 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG 3661 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG 3721 AGTCGCGGCG CTTACTTGTA CAGCTCGTCC ATGCCGCCGG TGGAGTGGCG GCCCTCGGCG 3781 CGTTCGTACT GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAACTTGATG 3841 TTGACGTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCTTGTAGGT GGTCTTGACC 3901 TCAGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCT GCTTGATCTC GCCCTTCAGG 3961 GCGCCGTCCT CGGGGTACAT CCGCTCGGAG GAGGCCTCCC AGCCCATGGT TTTCTTCTGC 4021 ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG 4081 CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC 4141 ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GATGTCGGCG 4201 GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC ATGAACTGAG GGGACAGGAT GTCCCAGGCG 4261 AAGGGCAGGG GGCCACCCTT GGTCACCTTC AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG 4321 CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC CTCCATGTGC 4381 ACCTTGAAGC GCATGAACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC CTTGCTCACC 4441 ATGGGCCGAT TGTTTAGCTT GTTCAGCTGC GCTTGTTTAT TTGCTTAGCT TTCGCTTAGC 4501 GACGTGTTCA CTTTGCTTGT TTGAATTGAA TTGTCGCTCC GTAGACGAAG CGCCTCTATT 4561 TATACTCCGG CGCTAATTGA ATTAGCTCTA ATTGAATTAG TCTCTAATTG AATGACGTCA 4621 TAACTTCGTA TAGCATACAT TATACGAAGT TATAGATCAC TAGTGATCTG AATTCATTTT 4681 CAACGTCCTC GATAGTATAG TGGTTAGTAT CCCCGCCTGT CACGCGGGAG ACCGGGGTTC 4741 AATTCCCCGT CGGGGAGAAT CTGTGATTCT TTTTTTTTTT CTTTTACTTT GTTATATAAA 4801 CAATTTTTGT TTTAATTGAA TCTAATTTGC CATTGCTTTT AGGAATCTCA GGCATCCAGC 4861 AAGCGTTTGT CCGCCGAATC GCCCATCAGT GAAGAAGATC CTGTGGCGGC TACGAAAATC 4921 TCCCCGGCCA TGTCGGCCTC CACCTCCAGC GAAAAACCCA TCAGCGAGCT GGCCACCTCT 4981 GTGCTGACCC ACCGCTTTCC AGACTCCACC TCCTCACCCG GCGAACATGG CCTTGGACGA 5041 ATGCAGTTGT CGATCCGCTA CAGCGCCCAG CGTCAAAAAC TAGACGTGAC CATACACAAA 5101 ATCCAGAAGA TACCACTTCG CGATCCCAGC AATATCCCCG ATCCGTATGT TAAGCTGTAT 5161 CTGTTGCCTG GACGCACCAA GGAGTCGAAA CGCAAGACGA GCGTGATCAA GGACAACTGC 5221 AACCCCGTCT ACGATGCATC CTTTGAGTAC CTGATTTCCA TTGCCGAACT CAGGCAGACG 5281 GAACTGGAGG TGACGGTGTG CACCCAAAAG GGATTCCTAT CCGGCGGTAG TCCCATCATT 5341 GGCATGGTAG GTACCCGAAA GCAACCCCTT AGTTACAGAC ACAGCGCGTA CGTCCTTCGC 5401 ATCCTTATGA TTCCCAAGTA CATATTCTGC AAGAGTACAG TATATATAGG AAAGATATCC 5461 GGGTGAACTT CGGGGTCTTC GAGAAGACCT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT 5521 AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTGCTCACCT 5581 GTGATTGCTC CTACTCAAAT ACAAAAACAT CAAATTTTCT GTCAATAAAG CATATTTATT 5641 TATATTTATT TTACAGGAAA GAATTCCTTT TAAAGTGTAT TTTAACCTAT AATGAAAAAC 5701 GATTAAAAAA AATACATAAA ATAATTCGAA AATTTTTGAA TAGCCCAGGT TGATAAAAAT 5761 TCATTTCATA CGTTTTATAA CTTATGCCCC TAAGTATTTT TTGACCATAG TGTTTCAATT 5821 CTACATTAAT TTTACAGAGT AGAATGAAAC GCCACCTACT CAGCCAAGAG GCGAAAAGGT 5881 TAGCTCGCCA AGCAGAGAGG GCGCCAGTGC TCACTACTTT TTATAATTCT CAACTTCTTT 5941 TTCCAGACTC AGTTCGTATA TATAGACCTA TTTTCAATTT AACGTCGGGG TCTTCGAGAA 6001 GACCTGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA 6061 AAAGTGGCAC CGAGTCGGTG CTTTTTTGCC TACCTGGAGC CTGAGAGTTG TTCAATAAAA 6121 TAAAAATGTT TCGTTTTTTT GCTTTCGCCA GTATTTATTA TTTTTCATCA ATATGTATTC 6181 AATTTGGTAT GTATTTAGTA ATTGTAATAT ATAGACAATG GTTTTCCGTT GACGTACATA 6241 CATCTGACGT GTGTTTATTT AGACATAATA GTTATGTTTT CACATCTTTT TAATGTTCGC 6301 TTAATGCGTA TGCTTAATTA ATATGTTCGG CTTGTCGACA TGCCCGCCGT GACCGTCGAG 6361 AACCCGCTGA CGCTGCCCCG CGTATCCGCA CCCGCCGACG CCGTCGCACG TCCCGTGCTC 6421 ACCGTGACCA CCGCGCCCAG CGGTTTCGAG GGCGAGGGCT TCCCGGTGCG CCGCGCGTTC 6481 GCCGGGATCA ACTACCGCCA CCTCGACCCG TTCATCATGA TGGACCAGAT GGGTGAGGTG 6541 GAGTACGCGC CCGGGGAGCC CAAGGGCACG CCCTGGCACC CG //