LOCUS pVH009 9446 bp ds-DNA circular 25-APR-2014 DEFINITION ASdel of pVH003 - AraC/pBad/CusR/LZX/pCusR/SH3/LZx/3xFlag-Venus YFP-AAV. ACCESSION urn.local.vhsiao.v-69ts5xo VERSION KEYWORDS . SOURCE ORGANISM . COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers promoter 242..479 /ApEinfo_fwdcolor="#008040" /ApEinfo_revcolor="#008040" /modified_by="User" /label="pBad" gene 559..1239 /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="#ff8040" /Color="#ff7400" /modified_by="User" /label="CusR" gene 1267..1394 /Original_Bases="AGCGTTAAAGAACTGGAAGATAAAAACGAAGAGCTCTTGAG CGAACTTTATCATTTGAAAAACGAAGTGGCGCGTCTGAAAAAGCTTGTTGGCGAACG" /Color="#056200" /modified_by="User" /label="LZx" misc_feature 1414..1542 /ApEinfo_fwdcolor="#be0c10" /ApEinfo_revcolor="#be0c10" /modified_by="User" /label="Double Terminator" promoter 1555..1645 /ApEinfo_fwdcolor="#37c8a0" /ApEinfo_revcolor="#37c8a0" /modified_by="User" /label="pCusR" misc_feature 1646..1688 /created_by="User" /modified_by="User" /label="RBS" gene 1689..2540 /Original_Bases="CGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCT GGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAA GGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGC CGGTTCCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCG TTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTAT GTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAG TGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAA AGAAGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTT TACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACA ACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGG TGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCT AAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGG GCATCACGCATGGTATGGATGAACTG" /Color="#ffe400" /modified_by="User" /label="Venus YFP" misc_feature 1692..1757 /created_by="User" /label="3x Flag" misc_feature 2505..2537 /created_by="User" /label="AAV Deg Tag" misc_feature 2553..2920 /ApEinfo_fwdcolor="#9191ff" /ApEinfo_revcolor="#9191ff" /label="TrrnB" CDS complement(3502..4293) /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /Color="#555555" /modified_by="User" /label="Kan/neoR" misc_feature 6546..7208 /ApEinfo_fwdcolor="#de9b69" /ApEinfo_revcolor="#de9b69" /label="pBBR1 ORI" misc_feature 8782..214 /ApEinfo_fwdcolor="#808000" /ApEinfo_revcolor="#808000" /label="AraC" ORIGIN 1 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc 61 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga 121 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc 181 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag 241 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 301 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 361 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 421 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 481 atcttacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ctagcggtac 541 ctcacgcgat acagatctat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa 601 tacttgacca aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg 661 aatggctacc atctggcgat gaccggtgat tatgatctga taatcctcga tattatgctg 721 ccggacgtga acggctggga tatcgtgcgc atgttacgct ccgccaataa agggatgccg 781 attctgttgc ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg 841 gcagatgact acctggtgaa gccattcgct tttgctgaac tgctggcgcg ggtgcgcaca 901 ttactgcggc gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatttgatg 961 gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc gcatcacttt gaccagtaaa 1021 gagtttactc tgctggagtt cttccttcgc catcagggcg aagtgctgcc ccgctcgctt 1081 atcgcctcgc aggtatggga catgaatttt gacagcgata ccaatgctat tgatgtggcg 1141 gtgaagcggc tgcgcggcaa aatcgacaac gactttgagc cgaagctaat tcagaccgtg 1201 cgcggcgtgg gttacatgct tgaggtgccg gatggtcagg gatctgggtc cggcagcggt 1261 ggatctctgg aaatccgcgc agcgtttctt gaaaaagaaa acaccgcact gcgtacccgc 1321 gcggccgaac tccgtaaacg tgtgggccgg tgccgcaaca ttgtatccaa atacgaaacc 1381 cgatacggcc cgctttaagg atcctgactc gagccaggca tcaaataaaa cgaaaggctc 1441 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1501 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taccgcggcc taggattggc 1561 aaaatgacaa ttttgtcatt tttctgtcac cggaaaatca gagcctggcg agtaaagttg 1621 gcggcataaa atcaccagaa attatgagct catacattcg taagaaacgg gcgcaggagg 1681 attagatgat ggactacaag gaccatgacg gtgattacaa ggatcatgac atcgactaca 1741 aggatgacga tgacaaggca actagcggca tggttagtaa aggagaagaa cttttcactg 1801 gagttgtccc aattttagtt gaactagatg gcgacgtgaa cggtcataag ttcagtgtct 1861 ccggcgaagg tgagggtgat gcaacgtacg gtaagttaac tttgaagtta atatgtacaa 1921 ccggcaagct gcctgttccc tggcctaccc tggtgacaac gttaggttat gggttgatgt 1981 gctttgctag atacccagat cacatgaaaa ggcatgactt ctttaaatct gcaatgccag 2041 aaggttacgt ccaagaacgt actattttct ttaaagatga cggtaattat aaaactaggg 2101 ctgaagttaa attcgaaggt gacacacttg taaatcgaat agagttaaag gggattgatt 2161 tcaaagagga tggtaatatt ctaggccata aacttgaata taactataat tcacacaacg 2221 tttacattac cgccgacaag cagaagaatg gaatcaaagc caattttaag attagacaca 2281 atattgagga tggtggagta cagcttgctg atcattacca acaaaatacc ccgatcggtg 2341 atggaccagt tttgctaccc gataaccatt atctgtccta tcaaagcaaa ttgtcaaaag 2401 atcctaacga aaaaagagac cacatggtac tcttggaatt tgtaacagct gctgggatta 2461 cacatggcat ggatgaacta tacaaaggtt ctggtaccgc agcagcaaac gacgaaaact 2521 acgctgcagc agtttaataa tacaaatagt gagaagcttg ggcccgaaca aaaactcatc 2581 tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg agtttaaacg 2641 gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat 2701 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc 2761 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt 2821 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa 2881 gactgggcct ttcgttttat ctgttgtttg tcggtgaact aatttctaga ccagcttttg 2941 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt 3001 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 3061 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 3121 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 3181 aggcggtttg cgtattgggc gcatgcataa aaactgttgt aattcattaa gcattctgcc 3241 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt 3301 gtcgccttgc gtataatatt tgcccatggg ggtgggcgaa gaactccagc atgagatccc 3361 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat 3421 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca 3481 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg 3541 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc 3601 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc 3661 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa 3721 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct 3781 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac 3841 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa 3901 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac 3961 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag 4021 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt 4081 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag 4141 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc 4201 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc 4261 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc 4321 ttgatcagat tttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt 4381 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct 4441 tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg 4501 ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccc 4561 aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg ggcctgtttc 4621 tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt gatgatcgcg 4681 gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac gggcttcagg 4741 cgctcccgaa ggtctcgggc cgtctcttgg gcttgatcgg ccttcttgcg catctcacgc 4801 gctcctgcgg cggcctgtag ggcaggctca tacccctgcc gaaccgcttt tgtcagccgg 4861 tcggccacgg cttccggcgt ctcaacgcgc tttgagattc ccagcttttc ggccaatccc 4921 tgcggtgcat aggcgcgtgg ctcgaccgct tgcgggctga tggtgacgtg gcccactggt 4981 ggccgctcca gggcctcgta gaacgcctga atgcgcgtgt gacgtgcctt gctgccctcg 5041 atgccccgtt gcagccctag atcggccaca gcggccgcaa acgtggtctg gtcgcgggtc 5101 atctgcgctt tgttgccgat gaactccttg gccgacagcc tgccgtcctg cgtcagcggc 5161 accacgaacg cggtcatgtg cgggctggtt tcgtcacggt ggatgctggc cgtcacgatg 5221 cgatccgccc cgtacttgtc cgccagccac ttgtgcgcct tctcgaagaa cgccgcctgc 5281 tgttcttggc tggccgactt ccaccattcc gggctggccg tcatgacgta ctcgaccgcc 5341 aacacagcgt ccttgcgccg cttctctggc agcaactcgc gcagtcggcc catcgcttca 5401 tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc tgctggcgtc agcgttgggc 5461 gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca cgttgcccat tttcgccagc 5521 ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc ctcccttttg gtgtccaacc 5581 ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc cactcaatgc ttgagtatac 5641 tcactagact ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg ccctacgggc 5701 ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt 5761 ggacgatggc cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg 5821 ctggacaagc tgatggacag gctgcgcctg cccacgagct tgaccacagg gattgcccac 5881 cggctaccca gccttcgacc acatacccac cggctccaac tgcgcggcct gcggccttgc 5941 cccatcaatt tttttaattt tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc 6001 gcttgccggt tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg 6061 gcttggcctt gacgcgcctg gaacgaccca agcctatgcg agtgggggca gtcgaaggcg 6121 aagcccgccc gcctgccccc cgagcctcac ggcggcgagt gcgggggttc caagggggca 6181 gcgccacctt gggcaaggcc gaaggccgcg cagtcgatca acaagccccg gaggggccac 6241 tttttgccgg agggggagcc gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct 6301 ttgggcacca aagaactaga tatagggcga aatgcgaaag acttaaaaat caacaactta 6361 aaaaaggggg gtacgcaaca gctcattgcg gcaccccccg caatagctca ttgcgtaggt 6421 taaagaaaat ctgtaattga ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc 6481 gaaaagttgc agctgattgc gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga 6541 taagcatggc cacgcagtcc agagaaatcg gcattcaagc caagaacaag cccggtcact 6601 gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca 6661 cggcggcaat gctgctgcat cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg 6721 tcagccagaa gacactttcc aagctcatcg gacgttcttt gcggacggtc caatacgcag 6781 tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg 6841 tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc 6901 tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga ccaggacgaa tcgctgttgg 6961 ggcatggcga cctgcgccgc atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg 7021 gccccggcga ggagccgccc agccagcccg gcattccggg catggaacca gacctgccag 7081 ccttgaccga aacggaggaa tgggaacggc gcgggcagca gcgcctgccg atgcccgatg 7141 agccgtgttt tctggacgat ggcgagccgt tggagccgcc gacacgggtc acgctgccgc 7201 gccggtagca cttgggttgc gcagcaaccc gtaagtgcgc tgttccagac tatcggctgt 7261 agccgcctcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc 7321 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accgttttta 7381 tcaggctctg ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag 7441 cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca 7501 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga 7561 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca 7621 ggcgtagcac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc 7681 tgccactcat cgcagtcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 7741 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact 7801 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 7861 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 7921 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctggggccgc 7981 aattcccaat tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 8041 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga 8101 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc 8161 caggaattaa ttccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 8221 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg 8281 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg 8341 ccaggaatta attccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 8401 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg 8461 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact 8521 gccaggaatt aattccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 8581 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc 8641 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac 8701 tgccaggaat tggggatcgg aagctgaatt cactagtcta tgctactcca tcgagccgtc 8761 aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc 8821 acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa 8881 atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt 8941 gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat 9001 ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc 9061 gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc 9121 gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag 9181 taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc 9241 ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg 9301 aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg 9361 cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg 9421 atgaatctct cctggcggga acagca //