LOCUS       pSMART-sgRNA (SP        2207 bp ds-DNA     circular SYN 15-JAN-2016
DEFINITION  High-copy number vector with a kanamycin resistance marker for 
            efficient blunt cloning of unstable sequences.
ACCESSION   AF532107
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Lucigen
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2016 from SnapGene 3.0.2 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     Linearize at HincII to make blunt cloning vector.
COMMENT     VNTDATE|275961600|
COMMENT     VNTDBDATE|758851200|
COMMENT     VNTNAME|pSMART(R) HCKan|
COMMENT     VNTAUTHORNAME|Lucigen|
FEATURES             Location/Qualifiers
     misc_feature    27..290
                     /label=U6
     terminator      484..515
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        516..618
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
     CDS             619..1434
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
     terminator      1458..1485
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1497..2084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2106..2135
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ccaagctcgg atccgatatc gacgtcaagg tcgggcagga agagggccta tttcccatga
       61 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
      121 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      181 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      241 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gggtcttcat
      301 tattccagaa gacaagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      361 tcaacttgaa aaagtggcac cgagtcggtg cttttttttg cggccgcact tgaaattcgg
      421 acgaattctc tagatatcgc tcaatactga ccatttaaat catacctgac ctccatagca
      481 gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag aggttccaac
      541 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
      601 ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc gaggccgcga
      661 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      721 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
      781 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag gctaaactgg
      841 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
      901 tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct
      961 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1021 cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgctca ggcgcaatca
     1081 cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa tggctggcct
     1141 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
     1201 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1261 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     1321 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     1381 aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctaatgaggg
     1441 cccaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc
     1501 cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga
     1561 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1621 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1921 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg
     1981 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2041 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt
     2101 tctaccgaag aaaggcccac ccgtgaaggt gagccagtga gttgattgca gtccagttac
     2161 gctggagtct gaggctcgtc ctgaatgata tcaagcttga attcgtt
//
