LOCUS Exported 11091 bp ds-DNA circular SYN 27-3月-2015 DEFINITION KW542_PB-TA-ERP2 (PB-tetO-RfA-rEf1a-rtTA-puro); All-in-One piggyBac transposon system with constitutive rtTA transactivator and puromycin resistance for dox-inducible expression of Gateway(R) cloned cDNAs. ACCESSION . VERSION . KEYWORDS KW542_PB-TA-ERP2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11091) AUTHORS Knut Woltjen TITLE Direct Submission JOURNAL Exported 2015年3月30日 from SnapGene 2.6.2 http://www.snapgene.com COMMENT Use LR Clonase(R) reaction to introduce a gene of interest from Gateway(R) Entry vector. FEATURES Location/Qualifiers source 1..11091 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=M13 origin /note="M13 origin" rep_origin 13..453 /label=F1 ori /note="F1 ori" CDS complement(463..531) /label=LacZ alpha /note="LacZ alpha" primer_bind 603..619 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(707..941) /gene="piggyBac 3' inverted terminal repeat" /bound_moiety="piggyBac Transposase" /note="PB 3'ITR" /note="Recombination site flanking the pigyBac transposon. Terminates in TTAA. Contains minimal 3' ITR and flanking sequence." protein_bind 1145..1163 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1182..1200 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1219..1237 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1256..1274 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1293..1311 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1330..1348 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1367..1385 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1404..1422 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 1431..1530 /label=mCMV promoter /note="mCMV promoter" protein_bind 1548..1672 /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR1" /note="recombination site for the Gateway(R) LR reaction" promoter 1697..1727 /note="lac UV5 promoter" /note="E. coli lac promoter with an ""up"" mutation" CDS 1781..2440 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" CDS 2782..3087 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="ccdB" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(3128..3252) /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR2" /note="recombination site for the Gateway(R) LR reaction" polyA_site 3370..3824 /note="beta-globin poly(A) signal" /note="rabbit beta-globin polyadenylation signal" enhancer 3928..4407 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 4408..5727 /label=rEF1a promoter /note="rEF1a promoter" CDS 5745..6491 /label=rtTA-Advanced /note="rtTA-Advanced" CDS 6499..7084 /note="IRES2" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 7085..7684 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 7769..8343 /note="EF-1-alpha poly(A) signal" /note="human EF-1-alpha polyadenylation signal" protein_bind 8442..8754 /gene="piggyBac 5' inverted terminal repeat" /bound_moiety="piggyBac Transposase" /note="PB 5'ITR" /note="Recombination site flanking the pigyBac transposon. Terminates in TTAA. Contains minimal 5' ITR and flanking sequence. May contain cryptic promoter activity." promoter complement(8900..8918) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8939..8955) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 8963..8979 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8987..9017) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 9032..9053 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(9341..9929) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10100..10960) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10961..11065) /gene="bla" /note="AmpR promoter" ORIGIN 1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg 661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct 961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt 1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac 1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca 1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga 1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct 1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac 1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat 1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag 1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca 1561 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc 1621 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc 1681 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag 1741 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg 1801 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc 1861 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac 1921 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat 1981 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag 2041 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag 2101 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta 2161 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc 2221 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt 2281 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 2341 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga 2401 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact 2461 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac 2521 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg 2581 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc 2641 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg 2701 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg 2761 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt 2821 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg 2881 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg 2941 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc 3001 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc 3061 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca 3121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg 3181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt 3241 gtacaaagtg gtgatggccg ctctagcgaa gggatccccc atcgaattca ctcctcaggt 3301 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca 3361 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 3421 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 3481 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 3541 ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa 3601 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct 3661 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc 3721 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag 3781 tcatagctgt ccctcttctc ttatgaagat ccctcgacct gcaggaattc gattacttaa 3841 gcctaggatc cagatctgta cattcgaatc gatgaattcg ttaaccatgg aagcttctgc 3901 agcggccgca ctagcggtgg cggcctcgac attgattatt gactagttat taatagtaat 3961 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 4021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 4081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 4141 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 4201 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 4261 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgatg atgcggtttt 4321 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 4381 ccattgacgt caatgggagt ttgttttgac tagtggagcc gagagtaatt catacaaaag 4441 gagggatcgc cttcgcaagg ggagagccca gggaccgtcc ctaaattctc acagacccaa 4501 atccctgtag ccgccccacg acagcgcgag gagcatgcgc ccagggctga gcgcgggtag 4561 atcagagcac acaagctcac agtccccggc ggtgggggga ggggcgcgct gagcgggggc 4621 cagggagctg gcgcggggca aactgggaaa gtggtgtcgt gtgctggctc cgccctcttc 4681 ccgagggtgg gggagaacgg tatataagtg cggtagtcgc cttggacgtt ctttttcgca 4741 acgggtttgc cgtcagaacg caggtgagtg gcgggtgtgg cttccgcggg ccccggagct 4801 ggagccctgc tctgagcggg ccgggctgat atgcgagtgt cgtccgcagg gtttagctgt 4861 gagcattccc acttcgagtg gcgggcggtg cgggggtgag agtgcgaggc ctagcggcaa 4921 ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcc gccgcgtgcc 4981 actccggccg cactatgcgt tttttgtcct tgctgccctc gattgccttc cagcagcatg 5041 ggctaacaaa gggagggtgt ggggctcact cttaaggagc ccatgaagct tacgttggat 5101 aggaatggaa gggcaggagg ggcgactggg gcccgcccgc cttcggagca catgtccgac 5161 gccacctgga tggggcgagg cctgtggctt tccgaagcaa tcgggcgtga gtttagccta 5221 cctgggccat gtggccctag cactgggcac ggtctggcct ggcggtgccg cgttcccttg 5281 cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt gcttgcgcgg aaagatggcc 5341 gctcccgggg ccctgttgca aggagctcaa aatggaggac gcggcagccc ggtggagcgg 5401 gcgggtgagt cacccacaca aaggaagagg gccttgcccc tcgccggccg ctgcttcctg 5461 tgaccccgtg gtctatcggc cgcatagtca cctcgggctt ctcttgagca ccgctcgtcg 5521 cggcgggggg aggggatcta atggcgttgg agtttgttca catttggtgg gtggagacta 5581 gtcaggccag cctggcgctg gaagtcattc ttggaatttg cccctttgag tttggagcga 5641 ggctaattct caagcctctt agcggttcaa aggtattttc taaacccgtt tccaggtgtt 5701 gtgaaagcca ccgctaattc aaagcaatcc ggagtaaatt caccatgtct agactggaca 5761 agagcaaagt cataaacggc gctctggaat tactcaatgg agtcggtatc gaaggcctga 5821 cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga 5881 agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc 5941 acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat 6001 tccgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa 6061 cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct 6121 ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat 6181 tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta 6241 tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct 6301 tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg 6361 gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg 6421 acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc 6481 tccccgggta aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg 6541 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 6601 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 6661 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 6721 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 6781 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 6841 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 6901 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 6961 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta 7021 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac 7081 aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc 7141 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc 7201 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 7261 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 7321 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 7381 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 7441 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 7501 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 7561 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 7621 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 7681 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc 7741 gaaatgaccg accaagcgaa ttcgctagga ttatccctaa tacctgccac cccactctta 7801 atcagtggtg gaagaacggt ctcagaactg tttgtttcaa ttggccattt aagtttagta 7861 gtaaaagact ggttaatgat aacaatgcat cgtaaaacct tcagaaggaa aggagaatgt 7921 tttgtggacc actttggttt tcttttttgc gtgtggcagt tttaagttat tagtttttaa 7981 aatcagtact ttttaatgga aacaacttga ccaaaaattt gtcacagaat tttgagaccc 8041 attaaaaaag ttaaatgaga aacctgtgtg ttcctttggt caacaccgag acatttaggt 8101 gaaagacatc taattctggt tttacgaatc tggaaacttc ttgaaaatgt aattcttgag 8161 ttaacacttc tgggtggaga atagggttgt tttcccccca cataattgga aggggaagga 8221 atatcattta aagctatggg agggttgctt tgattacaac actggagaga aatgcagcat 8281 gttgctgatt gcctgtcact aaaacaggcc aaaaactgag tccttgggtt gcatagaaag 8341 ctgccaaacg ctagccaaca agctcgtcat cgctttgcag aagagcagag aggatatgct 8401 catcgtctaa agaactaccc attttattat atattagtca cgatatctat aacaagaaaa 8461 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg 8521 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt 8581 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag 8641 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc 8701 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaaaaaga 8761 tttgcgcttt actcgaccta aactttaaac acgtcataga atcttcgttt gacaaaaacc 8821 acattgtggc caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc aagtcgcgcg 8881 aggtacccag cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt 8941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 9001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 9061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 9121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 9181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 9241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 9301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 9361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 9421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 9481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 9541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 9601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 9661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 9721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 9781 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 9841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 9901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 9961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 10021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 10081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 10141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 10201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 10261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 10321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 10381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 10441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 10501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 10561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 10621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 10681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 10741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 10801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 10861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 10921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 10981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 11041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g //