LOCUS       Exported               11091 bp ds-DNA     circular SYN 27-3月-2015
DEFINITION  KW542_PB-TA-ERP2 (PB-tetO-RfA-rEf1a-rtTA-puro); All-in-One piggyBac 
            transposon system with constitutive rtTA transactivator and 
            puromycin resistance for dox-inducible expression of Gateway(R) 
            cloned cDNAs.
ACCESSION   .
VERSION     .
KEYWORDS    KW542_PB-TA-ERP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Knut Woltjen
  TITLE     Direct Submission
  JOURNAL   Exported 2015年3月30日 from SnapGene 2.6.2
            http://www.snapgene.com
COMMENT     Use LR Clonase(R) reaction to introduce a gene of interest from 
            Gateway(R) Entry vector.
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=M13 origin
                     /note="M13 origin"
     rep_origin      13..453
                     /label=F1 ori
                     /note="F1 ori"
     CDS             complement(463..531)
                     /label=LacZ alpha
                     /note="LacZ alpha"
     primer_bind     603..619
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(707..941)
                     /gene="piggyBac 3' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 3'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 3' ITR and flanking 
                     sequence."
     protein_bind    1145..1163
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1431..1530
                     /label=mCMV promoter
                     /note="mCMV promoter"
     protein_bind    1548..1672
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1697..1727
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1781..2440
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2782..3087
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3128..3252)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_site      3370..3824
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     enhancer        3928..4407
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4408..5727
                     /label=rEF1a promoter
                     /note="rEF1a promoter"
     CDS             5745..6491
                     /label=rtTA-Advanced
                     /note="rtTA-Advanced"
     CDS             6499..7084
                     /note="IRES2"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7085..7684
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    7769..8343
                     /note="EF-1-alpha poly(A) signal"
                     /note="human EF-1-alpha polyadenylation signal"
     protein_bind    8442..8754
                     /gene="piggyBac 5' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 5'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 5' ITR and flanking 
                     sequence. May contain cryptic promoter activity."
     promoter        complement(8900..8918)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8939..8955)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8963..8979
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8987..9017)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9032..9053
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9341..9929)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10100..10960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10961..11065)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     1621 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     1681 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     1741 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1801 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1861 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1921 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1981 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2041 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2101 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2161 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     2221 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2281 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2341 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     2401 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact
     2461 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     2521 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     2581 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     2641 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     2701 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     2761 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     2821 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     2881 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     2941 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     3001 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     3061 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     3121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     3181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     3241 gtacaaagtg gtgatggccg ctctagcgaa gggatccccc atcgaattca ctcctcaggt
     3301 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     3361 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     3421 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     3481 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     3541 ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa
     3601 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     3661 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     3721 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     3781 tcatagctgt ccctcttctc ttatgaagat ccctcgacct gcaggaattc gattacttaa
     3841 gcctaggatc cagatctgta cattcgaatc gatgaattcg ttaaccatgg aagcttctgc
     3901 agcggccgca ctagcggtgg cggcctcgac attgattatt gactagttat taatagtaat
     3961 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     4021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     4081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     4141 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     4201 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     4261 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgatg atgcggtttt
     4321 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4381 ccattgacgt caatgggagt ttgttttgac tagtggagcc gagagtaatt catacaaaag
     4441 gagggatcgc cttcgcaagg ggagagccca gggaccgtcc ctaaattctc acagacccaa
     4501 atccctgtag ccgccccacg acagcgcgag gagcatgcgc ccagggctga gcgcgggtag
     4561 atcagagcac acaagctcac agtccccggc ggtgggggga ggggcgcgct gagcgggggc
     4621 cagggagctg gcgcggggca aactgggaaa gtggtgtcgt gtgctggctc cgccctcttc
     4681 ccgagggtgg gggagaacgg tatataagtg cggtagtcgc cttggacgtt ctttttcgca
     4741 acgggtttgc cgtcagaacg caggtgagtg gcgggtgtgg cttccgcggg ccccggagct
     4801 ggagccctgc tctgagcggg ccgggctgat atgcgagtgt cgtccgcagg gtttagctgt
     4861 gagcattccc acttcgagtg gcgggcggtg cgggggtgag agtgcgaggc ctagcggcaa
     4921 ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcc gccgcgtgcc
     4981 actccggccg cactatgcgt tttttgtcct tgctgccctc gattgccttc cagcagcatg
     5041 ggctaacaaa gggagggtgt ggggctcact cttaaggagc ccatgaagct tacgttggat
     5101 aggaatggaa gggcaggagg ggcgactggg gcccgcccgc cttcggagca catgtccgac
     5161 gccacctgga tggggcgagg cctgtggctt tccgaagcaa tcgggcgtga gtttagccta
     5221 cctgggccat gtggccctag cactgggcac ggtctggcct ggcggtgccg cgttcccttg
     5281 cctcccaaca agggtgaggc cgtcccgccc ggcaccagtt gcttgcgcgg aaagatggcc
     5341 gctcccgggg ccctgttgca aggagctcaa aatggaggac gcggcagccc ggtggagcgg
     5401 gcgggtgagt cacccacaca aaggaagagg gccttgcccc tcgccggccg ctgcttcctg
     5461 tgaccccgtg gtctatcggc cgcatagtca cctcgggctt ctcttgagca ccgctcgtcg
     5521 cggcgggggg aggggatcta atggcgttgg agtttgttca catttggtgg gtggagacta
     5581 gtcaggccag cctggcgctg gaagtcattc ttggaatttg cccctttgag tttggagcga
     5641 ggctaattct caagcctctt agcggttcaa aggtattttc taaacccgtt tccaggtgtt
     5701 gtgaaagcca ccgctaattc aaagcaatcc ggagtaaatt caccatgtct agactggaca
     5761 agagcaaagt cataaacggc gctctggaat tactcaatgg agtcggtatc gaaggcctga
     5821 cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga
     5881 agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc
     5941 acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat
     6001 tccgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa
     6061 cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct
     6121 ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat
     6181 tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta
     6241 tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct
     6301 tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg
     6361 gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg
     6421 acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc
     6481 tccccgggta aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     6541 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     6601 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     6661 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     6721 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     6781 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     6841 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     6901 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     6961 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     7021 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     7081 aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     7141 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     7201 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     7261 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     7321 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     7381 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     7441 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     7501 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7561 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7621 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7681 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     7741 gaaatgaccg accaagcgaa ttcgctagga ttatccctaa tacctgccac cccactctta
     7801 atcagtggtg gaagaacggt ctcagaactg tttgtttcaa ttggccattt aagtttagta
     7861 gtaaaagact ggttaatgat aacaatgcat cgtaaaacct tcagaaggaa aggagaatgt
     7921 tttgtggacc actttggttt tcttttttgc gtgtggcagt tttaagttat tagtttttaa
     7981 aatcagtact ttttaatgga aacaacttga ccaaaaattt gtcacagaat tttgagaccc
     8041 attaaaaaag ttaaatgaga aacctgtgtg ttcctttggt caacaccgag acatttaggt
     8101 gaaagacatc taattctggt tttacgaatc tggaaacttc ttgaaaatgt aattcttgag
     8161 ttaacacttc tgggtggaga atagggttgt tttcccccca cataattgga aggggaagga
     8221 atatcattta aagctatggg agggttgctt tgattacaac actggagaga aatgcagcat
     8281 gttgctgatt gcctgtcact aaaacaggcc aaaaactgag tccttgggtt gcatagaaag
     8341 ctgccaaacg ctagccaaca agctcgtcat cgctttgcag aagagcagag aggatatgct
     8401 catcgtctaa agaactaccc attttattat atattagtca cgatatctat aacaagaaaa
     8461 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     8521 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     8581 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     8641 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     8701 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaaaaaga
     8761 tttgcgcttt actcgaccta aactttaaac acgtcataga atcttcgttt gacaaaaacc
     8821 acattgtggc caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc aagtcgcgcg
     8881 aggtacccag cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt
     8941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9781 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
