LOCUS       Exported               12326 bp ds-DNA     circular SYN 27-3月-2015
DEFINITION  KW543_PB-TAC-ERP2 (PB-tetO-RfA-mCherry-rEf1a-rtTA-puro); All-in-One 
            piggyBac transposon system with constitutive rtTA transactivator and
            puromycin resistance for dox-inducible expression of Gateway(R) 
            cloned cDNAs with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    KW543_PB-TAC-ERP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Knut Woltjen
  TITLE     Direct Submission
  JOURNAL   Exported 2015年3月30日 from SnapGene 2.6.2
            http://www.snapgene.com
COMMENT     Use LR Clonase(R) reaction to introduce a gene of interest from 
            Gateway(R) Entry vector.
FEATURES             Location/Qualifiers
     source          1..12326
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=M13 origin
                     /note="M13 origin"
     rep_origin      13..453
                     /label=F1 ori
                     /note="F1 ori"
     CDS             complement(463..531)
                     /label=LacZ alpha
                     /note="LacZ alpha"
     primer_bind     603..619
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(707..941)
                     /gene="piggyBac 3' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 3'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 3' ITR and flanking 
                     sequence."
     protein_bind    1145..1163
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1431..1530
                     /label=mCMV promoter
                     /note="mCMV promoter"
     protein_bind    1548..1672
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1697..1727
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1781..2440
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2782..3087
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3128..3252)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3294..3879
                     /note="IRES2"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3880..4590
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_site      4605..5059
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     enhancer        5163..5642
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5643..6962
                     /label=rEF1a promoter
                     /note="rEF1a promoter"
     CDS             6980..7726
                     /label=rtTA-Advanced
                     /note="rtTA-Advanced"
     CDS             7734..8319
                     /note="IRES2"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             8320..8919
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    9004..9578
                     /note="EF-1-alpha poly(A) signal"
                     /note="human EF-1-alpha polyadenylation signal"
     protein_bind    9677..9989
                     /gene="piggyBac 5' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 5'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 5' ITR and flanking 
                     sequence. May contain cryptic promoter activity."
     promoter        complement(10135..10153)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10174..10190)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10198..10214
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10222..10252)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10267..10288
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10576..11164)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11335..12195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12196..12300)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     1621 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     1681 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     1741 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1801 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1861 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1921 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1981 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2041 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2101 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2161 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     2221 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2281 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     2341 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     2401 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact
     2461 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     2521 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     2581 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     2641 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     2701 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     2761 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     2821 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     2881 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     2941 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     3001 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     3061 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     3121 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     3181 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     3241 gtacaaagtg gtgatctagc taaaacgcgg cctcgaatcg aattccgccc ccccccctct
     3301 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     3361 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     3421 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     3481 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     3541 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     3601 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     3661 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     3721 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     3781 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     3841 ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag
     3901 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3961 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     4021 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     4081 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     4141 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4201 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     4261 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     4321 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     4381 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     4441 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     4501 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     4561 accggcggca tggacgagct gtacaagtaa agcggccgcg atctttttcc ctctgccaaa
     4621 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     4681 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     4741 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     4801 atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc
     4861 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     4921 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4981 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     5041 aagatccctc gacctgcagg aattcgatta cttaagccta ggatccagat ctgtacattc
     5101 gaatcgatga attcgttaac catggaagct tctgcagcgg ccgcactagc ggtggcggcc
     5161 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     5221 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     5281 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     5341 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     5401 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     5461 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     5521 attagtcatc gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata
     5581 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     5641 ttgactagtg gagccgagag taattcatac aaaaggaggg atcgccttcg caaggggaga
     5701 gcccagggac cgtccctaaa ttctcacaga cccaaatccc tgtagccgcc ccacgacagc
     5761 gcgaggagca tgcgcccagg gctgagcgcg ggtagatcag agcacacaag ctcacagtcc
     5821 ccggcggtgg ggggaggggc gcgctgagcg ggggccaggg agctggcgcg gggcaaactg
     5881 ggaaagtggt gtcgtgtgct ggctccgccc tcttcccgag ggtgggggag aacggtatat
     5941 aagtgcggta gtcgccttgg acgttctttt tcgcaacggg tttgccgtca gaacgcaggt
     6001 gagtggcggg tgtggcttcc gcgggccccg gagctggagc cctgctctga gcgggccggg
     6061 ctgatatgcg agtgtcgtcc gcagggttta gctgtgagca ttcccacttc gagtggcggg
     6121 cggtgcgggg gtgagagtgc gaggcctagc ggcaaccccg tagcctcgcc tcgtgtccgg
     6181 cttgaggcct agcgtggtgt ccgccgccgc gtgccactcc ggccgcacta tgcgtttttt
     6241 gtccttgctg ccctcgattg ccttccagca gcatgggcta acaaagggag ggtgtggggc
     6301 tcactcttaa ggagcccatg aagcttacgt tggataggaa tggaagggca ggaggggcga
     6361 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgt
     6421 ggctttccga agcaatcggg cgtgagttta gcctacctgg gccatgtggc cctagcactg
     6481 ggcacggtct ggcctggcgg tgccgcgttc ccttgcctcc caacaagggt gaggccgtcc
     6541 cgcccggcac cagttgcttg cgcggaaaga tggccgctcc cggggccctg ttgcaaggag
     6601 ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg tgagtcaccc acacaaagga
     6661 agagggcctt gcccctcgcc ggccgctgct tcctgtgacc ccgtggtcta tcggccgcat
     6721 agtcacctcg ggcttctctt gagcaccgct cgtcgcggcg gggggagggg atctaatggc
     6781 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     6841 cattcttgga atttgcccct ttgagtttgg agcgaggcta attctcaagc ctcttagcgg
     6901 ttcaaaggta ttttctaaac ccgtttccag gtgttgtgaa agccaccgct aattcaaagc
     6961 aatccggagt aaattcacca tgtctagact ggacaagagc aaagtcataa acggcgctct
     7021 ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     7081 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     7141 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     7201 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
     7261 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     7321 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     7381 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     7441 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     7501 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     7561 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg cccttgacga
     7621 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     7681 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaaaatt ccgcccctct
     7741 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     7801 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     7861 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     7921 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     7981 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     8041 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     8101 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     8161 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     8221 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     8281 ttttcctttg aaaaacacga tgataatatg gccacaacca tgaccgagta caagcccacg
     8341 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     8401 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8461 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8521 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8581 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8641 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8701 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8761 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8821 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8881 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     8941 cgcccgaccg aaaggagcgc acgaccccat gcatcgaaat gaccgaccaa gcgaattcgc
     9001 taggattatc cctaatacct gccaccccac tcttaatcag tggtggaaga acggtctcag
     9061 aactgtttgt ttcaattggc catttaagtt tagtagtaaa agactggtta atgataacaa
     9121 tgcatcgtaa aaccttcaga aggaaaggag aatgttttgt ggaccacttt ggttttcttt
     9181 tttgcgtgtg gcagttttaa gttattagtt tttaaaatca gtacttttta atggaaacaa
     9241 cttgaccaaa aatttgtcac agaattttga gacccattaa aaaagttaaa tgagaaacct
     9301 gtgtgttcct ttggtcaaca ccgagacatt taggtgaaag acatctaatt ctggttttac
     9361 gaatctggaa acttcttgaa aatgtaattc ttgagttaac acttctgggt ggagaatagg
     9421 gttgttttcc ccccacataa ttggaagggg aaggaatatc atttaaagct atgggagggt
     9481 tgctttgatt acaacactgg agagaaatgc agcatgttgc tgattgcctg tcactaaaac
     9541 aggccaaaaa ctgagtcctt gggttgcata gaaagctgcc aaacgctagc caacaagctc
     9601 gtcatcgctt tgcagaagag cagagaggat atgctcatcg tctaaagaac tacccatttt
     9661 attatatatt agtcacgata tctataacaa gaaaatatat atataataag ttatcacgta
     9721 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     9781 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     9841 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     9901 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     9961 ttttacgcag actatctttc tagggttaaa aaagatttgc gctttactcg acctaaactt
    10021 taaacacgtc atagaatctt cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc
    10081 gacgcgcgct aaagaatggc aaaccaagtc gcgcgaggta cccagctttt gttcccttta
    10141 gtgagggtta attccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    10201 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10261 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    10321 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    10381 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    10441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    10501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    10561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10981 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    11041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    11461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    11521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    11581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    11641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    11761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    12121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    12181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12241 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    12301 cacatttccc cgaaaagtgc cacctg
//
