LOCUS Exported 11380 bp ds-DNA circular SYN 27-3月-2015 DEFINITION KW031_PB-TAC-OKMS; piggyBac transposon system for dox-inducible iPS cell generation (OKMS cassette with mCherry reporter). ACCESSION . VERSION . KEYWORDS KW031_PB-TAC-OKMS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11380) AUTHORS Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, Yamamoto T, Yamanaka S, Woltjen K. TITLE KLF4 N-Terminal Variance Modulates Induced Reprogramming to Pluripotency. JOURNAL Stem Cell Reports 2015. PUBMED 25772473 REFERENCE 2 (bases 1 to 11380) AUTHORS Knut Woltjen TITLE Direct Submission JOURNAL Exported 2015年3月30日 from SnapGene 2.6.2 http://www.snapgene.com COMMENT Requires piggyBac transposase for genomic integration. FEATURES Location/Qualifiers source 1..11380 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=M13 origin /note="M13 origin" rep_origin 13..453 /label=F1 ori /note="F1 ori" CDS complement(463..531) /label=LacZ alpha /note="LacZ alpha" primer_bind 603..619 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(707..941) /gene="piggyBac 3' inverted terminal repeat" /bound_moiety="piggyBac Transposase" /note="PB 3'ITR" /note="Recombination site flanking the pigyBac transposon. Terminates in TTAA. Contains minimal 3' ITR and flanking sequence." protein_bind 1145..1163 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1182..1200 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1219..1237 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1256..1274 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1293..1311 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1330..1348 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1367..1385 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1404..1422 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 1431..1530 /label=mCMV promoter /note="mCMV promoter" protein_bind 1548..1572 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB1" /note="recombination site for the Gateway(R) BP reaction" CDS 1645..6735 /codon_start=1 /note="OKMS cassette" /translation="MAGHLASDFAFSPPPGGGDGSAGLEPGWVDSRTWLSFQGPPGGPG IGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG TSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLT LGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQA RKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRS SIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSV PVTALGSPMHSNGDLKIVAPVKQTLNFDLLKLAGDVESNPGPLMAVSDALLPSFSTFAS GPAGREKTLRPAGAPTNRWREELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSN NPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPS TCSFSYPIRAGGDPGVAASNTGGGLLYSRESAPPPTAPFNLADINDVSPSGGFVAELLR PELDPVYIPPQQPQPPGGGLMGKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVA PYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTT PTLSPEELLNSRDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPSLHYQELMPPGSCL PEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCG WKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFLEGSGQCTNYALLK LAGDVESNPGPGAPLDFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPYFICDEEEN FYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDG GGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSE KLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPK SCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVS VEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRA KLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPEL ENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGAGDLK IVAPVKQTLNFDLLKLAGDVESNPGPLMNNSPFTMYNMMETELKPPGPQQASGGGGGGG NATAAATGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLL SETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMAS GVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRY DVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSS HSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM" CDS 1648..2700 /codon_start=1 /note="Oct3/4" /note="REFSEQ:NM_013633.3" /translation="AGHLASDFAFSPPPGGGDGSAGLEPGWVDSRTWLSFQGPPGGPGI GPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGT SSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTL GVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQAR KRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSS IEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVP VTALGSPMHSN" variation 1735 /note="rs8252753" /note="Missense variant" /db_xref="dbSNP:rs8252753" CDS 2725..2790 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /note="F2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" CDS 2797..4215 /codon_start=1 /note="Klf4" /note="REFSEQ:NM_010637.3;Klf4-S" /translation="AVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRLP PLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQ ESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAASNTGGGLLYSRE SAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASL TTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAH LSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPGP NYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCGK TYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRA FSRSDHLALHMKRHF" variation 3165 /note="rs16802583" /note="Synonymous variant" /db_xref="dbSNP:rs16802583" variation 4161 /note="rs32168644" /note="Synonymous variant" /db_xref="dbSNP:rs32168644" CDS 4231..4290 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /note="E2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" CDS 4303..5661 /codon_start=1 /note="cMyc" /note="REFSEQ:NM_010849.4;isoform a;non-canonical start" /translation="DFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPYFICDEEENF YHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGG GGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEK LASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKS CTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSV EKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAK LDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELE NNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA" CDS 5686..5751 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /note="F2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" CDS 5779..6732 /codon_start=1 /note="Sox2" /note="REFSEQ:NM_011443.3" /translation="YNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKRP MNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMK EHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAH MNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSP TYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPG AEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM" protein_bind complement(6791..6815) /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB2" /note="recombination site for the Gateway(R) BP reaction" CDS 6857..7442 /note="IRES2" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 7443..8153 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mCherry" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" polyA_site 8168..8622 /note="beta-globin poly(A) signal" /note="rabbit beta-globin polyadenylation signal" protein_bind 8731..9043 /gene="piggyBac 5' inverted terminal repeat" /bound_moiety="piggyBac Transposase" /note="PB 5'ITR" /note="Recombination site flanking the pigyBac transposon. Terminates in TTAA. Contains minimal 5' ITR and flanking sequence. May contain cryptic promoter activity." promoter complement(9189..9207) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9228..9244) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 9252..9268 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9276..9306) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 9321..9342 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(9630..10218) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10389..11249) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(11250..11354) /gene="bla" /note="AmpR promoter" ORIGIN 1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg 661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct 961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt 1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac 1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca 1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga 1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct 1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac 1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat 1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag 1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca 1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccct 1621 taaaaagcag gctccacctt ccccatggct ggacacctgg cttcagactt cgccttctca 1681 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggattctcga 1741 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta 1801 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga 1861 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc 1921 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac 1981 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg 2041 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc 2101 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc 2161 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag 2221 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag 2281 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac 2341 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag 2401 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt 2461 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag 2521 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac 2581 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct 2641 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac 2701 ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg 2761 gctggggatg tagaaagcaa tccaggtcca ctcatggctg tcagcgacgc tctgctcccg 2821 tccttctcca cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt 2881 gccccgacta accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc 2941 ggccgcccct acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt 3001 gcagcttgca gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac 3061 gacctcctgg acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc 3121 gccaccgtga ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct 3181 gccagcgcgc cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc 3241 gtggctgcca gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc 3301 acggccccct tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct 3361 gagctcctgc ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca 3421 ggtggcgggc tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag 3481 tacagcagcc cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg 3541 gtagtggcgc cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg 3601 gtcccgtcct gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc 3661 agcaacggcc accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg 3721 actaccccta cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg 3781 cctcttcccc caggattcca tccccatccg gggcccaact accctccttt cctgccagac 3841 cagatgcagt cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc 3901 ctgccagagg agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc 3961 acccacactt gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag 4021 gcacacctgc gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg 4081 tggaaattcg cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg 4141 ccctttcagt gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac 4201 atgaagaggc actttctcga gggctcgggc cagtgtacta attatgctct cttgaaattg 4261 gctggagatg ttgagagcaa cccaggtccc ggcgcgccac tggatttcct ttgggcgttg 4321 gaaaccccgc agacagccac gacgatgccc ctcaacgtga acttcaccaa caggaactat 4381 gacctcgact acgactccgt acagccctat ttcatctgcg acgaggaaga gaatttctat 4441 caccagcaac agcagagcga gctgcagccg cccgcgccca gtgaggatat ctggaagaaa 4501 ttcgagctgc ttcccacccc gcccctgtcc ccgagccgcc gctccgggct ctgctctcca 4561 tcctatgttg cggtcgctac gtccttctcc ccaagggaag acgatgacgg cggcggtggc 4621 aacttctcca ccgccgatca gctggagatg atgaccgagt tacttggagg agacatggtg 4681 aaccagagct tcatctgcga tcctgacgac gagaccttca tcaagaacat catcatccag 4741 gactgtatgt ggagcggttt ctcagccgct gccaagctgg tctcggagaa gctggcctcc 4801 taccaggctg cgcgcaaaga cagcaccagc ctgagccccg cccgcgggca cagcgtctgc 4861 tccacctcca gcctgtacct gcaggacctc accgccgccg cgtccgagtg cattgacccc 4921 tcagtggtct ttccctaccc gctcaacgac agcagctcgc ccaaatcctg tacctcgtcc 4981 gattccacgg ccttctctcc ttcctcggac tcgctgctgt cctccgagtc ctccccacgg 5041 gccagccctg agcccctagt gctgcatgag gagacaccgc ccaccaccag cagcgactct 5101 gaagaagagc aagaagatga ggaagaaatt gatgtggtgt ctgtggagaa gaggcaaacc 5161 cctgccaaga ggtcggagtc gggctcatct ccatcccgag gccacagcaa acctccgcac 5221 agcccactgg tcctcaagag gtgccacgtc tccactcacc agcacaacta cgccgcaccc 5281 ccctccacaa ggaaggacta tccagctgcc aagagggcca agttggacag tggcagggtc 5341 ctgaagcaga tcagcaacaa ccgcaagtgc tccagcccca ggtcctcaga cacggaggaa 5401 aacgacaaga ggcggacaca caacgtcttg gaacgtcaga ggaggaacga gctgaagcgc 5461 agcttttttg ccctgcgtga ccagatccct gaattggaaa acaacgaaaa ggcccccaag 5521 gtagtgatcc tcaaaaaagc caccgcctac atcctgtcca ttcaagcaga cgagcacaag 5581 ctcacctctg aaaaggactt attgaggaaa cgacgagaac agttgaaaca caaactcgaa 5641 cagcttcgaa actctggtgc aggggatctc aaaattgtcg ctcctgtcaa acaaactctt 5701 aactttgatt tactcaaact ggctggggat gtagaaagca atccaggtcc actcatgaac 5761 aattcgccct tcaccatgta taacatgatg gagacggagc tgaagccgcc gggcccgcag 5821 caagcttcgg ggggcggcgg cggaggaggc aacgccacgg cggcggcgac cggcggcaac 5881 cagaagaaca gcccggaccg cgtcaagagg cccatgaacg ccttcatggt atggtcccgg 5941 gggcagcggc gtaagatggc ccaggagaac cccaagatgc acaactcgga gatcagcaag 6001 cgcctgggcg cggagtggaa acttttgtcc gagaccgaga agcggccgtt catcgacgag 6061 gccaagcggc tgcgcgctct gcacatgaag gagcacccgg attataaata ccggccgcgg 6121 cggaaaacca agacgctcat gaagaaggat aagtacacgc ttcccggagg cttgctggcc 6181 cccggcggga acagcatggc gagcggggtt ggggtgggcg ccggcctggg tgcgggcgtg 6241 aaccagcgca tggacagcta cgcgcacatg aacggctgga gcaacggcag ctacagcatg 6301 atgcaggagc agctgggcta cccgcagcac ccgggcctca acgctcacgg cgcggcacag 6361 atgcaaccga tgcaccgcta cgacgtcagc gccctgcagt acaactccat gaccagctcg 6421 cagacctaca tgaacggctc gcccacctac agcatgtcct actcgcagca gggcaccccc 6481 ggtatggcgc tgggctccat gggctctgtg gtcaagtccg aggccagctc cagccccccc 6541 gtggttacct cttcctccca ctccagggcg ccctgccagg ccggggacct ccgggacatg 6601 atcagcatgt acctccccgg cgccgaggtg ccggagcccg ctgcgcccag tagactgcac 6661 atggcccagc actaccagag cggcccggtg cccggcacgg ccattaacgg cacactgccc 6721 ctgtcgcaca tgtgagggct ggaagggcga attgcctgca ggaattcgcg gccgcactcg 6781 agatatctag acccagcttt cttgtacaaa gtggtgatct agctaaaacg cggcctcgaa 6841 tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct 6901 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg 6961 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt 7021 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg 7081 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac 7141 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg 7201 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct 7261 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg 7321 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg 7381 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa 7441 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca 7501 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 7561 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc 7621 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc 7681 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc 7741 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg 7801 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg 7861 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg 7921 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg 7981 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg 8041 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 8101 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc 8161 gcgatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga 8221 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc 8281 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg 8341 tttagagttt ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga 8401 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 8461 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 8521 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 8581 atagctgtcc ctcttctctt atgaagatcc ctcgacctgc aggaattcga tatcaagctc 8641 tagccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc atcgtctaaa 8701 gaactaccca ttttattata tattagtcac gatatctata acaagaaaat atatatataa 8761 taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt 8821 aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa 8881 atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag 8941 atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag 9001 aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat ttgcgcttta 9061 ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc 9121 aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga ggtacccagc 9181 ttttgttccc tttagtgagg gttaattccg agcttggcgt aatcatggtc atagctgttt 9241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 10021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 10081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 11041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11341 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg //