LOCUS       Exported               11309 bp ds-DNA     circular SYN 27-3月-2015
DEFINITION  KW025_PB-TAC-OSKM; piggyBac transposon system for dox-inducible iPS 
            cell generation (OSKM cassette with mCherry reporter).
ACCESSION   .
VERSION     .
KEYWORDS    KW025_PB-TAC-OSKM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K.
  TITLE     KLF4 N-Terminal Variance Modulates Induced Reprogramming to 
            Pluripotency.
  JOURNAL   Stem Cell Reports 2015.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Knut Woltjen
  TITLE     Direct Submission
  JOURNAL   Exported 2015年3月30日 from SnapGene 2.6.2
            http://www.snapgene.com
COMMENT     Requires piggyBac transposase for genomic integration.
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=M13 origin
                     /note="M13 origin"
     rep_origin      13..453
                     /label=F1 ori
                     /note="F1 ori"
     CDS             complement(463..531)
                     /label=LacZ alpha
                     /note="LacZ alpha"
     primer_bind     603..619
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(707..941)
                     /gene="piggyBac 3' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 3'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 3' ITR and flanking 
                     sequence."
     protein_bind    1145..1163
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1431..1530
                     /label=mCMV promoter
                     /note="mCMV promoter"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1619..6682
                     /codon_start=1
                     /note="OSKM cassette"
                     /translation="MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPG
                     IGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG
                     TSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLT
                     LGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQA
                     RKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRS
                     SIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSV
                     PVTALGSPMHSNASGSGATNFSLLKQAGDVEENPGPACMYNMMETELKPPGPQQASGGG
                     GGGGNATAAATGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAE
                     WKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGN
                     SMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQP
                     MHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVV
                     TSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPL
                     SHMACGSGEGRGSLLTCGDVEENPGPLEMRQPPGESDMAVSDALLPSFSTFASGPAGRE
                     KTLRPAGAPTNRWREELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSNNPALLA
                     RRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPSTCSFSY
                     PIRAGGDPGVAASNTGGGLLYSRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPV
                     YIPPQQPQPPGGGLMGKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGP
                     PRMCPKIKQEAVPSCTVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPE
                     ELLNSRDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKP
                     KRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARS
                     DELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFLEGSGQCTNYALLKLAGDVE
                     SNPGPGAPLDFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQ
                     QSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFS
                     TADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQ
                     AARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSD
                     STAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQT
                     PAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGR
                     VLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKA
                     PKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA"
     CDS             1622..2674
                     /codon_start=1
                     /note="Oct3/4"
                     /note="REFSEQ:NM_013633.3"
                     /translation="AGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGI
                     GPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGT
                     SSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTL
                     GVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQAR
                     KRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSS
                     IEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVP
                     VTALGSPMHSN"
     CDS             2690..2746
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2756..3709
                     /codon_start=1
                     /note="Sox2"
                     /note="REFSEQ:NM_011443.3"
                     /translation="YNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKRP
                     MNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMK
                     EHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAH
                     MNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSP
                     TYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPG
                     AEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM"
     CDS             3725..3778
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     variation       3785..3811
                     /note="+9aa"
                     /note="GENBANK:U20344.1"
     CDS             3788..5233
                     /codon_start=1
                     /note="Klf4"
                     /note="REFSEQ:NM_010637.3;Klf4-S"
                     /translation="RQPPGESDMAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWRE
                     ELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDF
                     ILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAASNT
                     GGGLLYSRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLM
                     GKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSC
                     TVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLP
                     PGFHPHPGPNYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATH
                     TCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRP
                     FQCQKCDRAFSRSDHLALHMKRHF"
     variation       4183
                     /note="rs16802583"
                     /note="Synonymous variant"
                     /db_xref="dbSNP:<html><body>rs16802583</body></html>"
     variation       5179
                     /note="rs32168644"
                     /note="Synonymous variant"
                     /db_xref="dbSNP:<html><body>rs32168644</body></html>"
     CDS             5249..5308
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /note="E2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5321..6679
                     /codon_start=1
                     /note="cMyc"
                     /note="REFSEQ:NM_010849.4;isoform a;non-canonical start"
                     /translation="DFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPYFICDEEENF
                     YHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGG
                     GGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEK
                     LASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKS
                     CTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSV
                     EKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAK
                     LDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELE
                     NNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA"
     protein_bind    complement(6720..6744)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6786..7371
                     /note="IRES2"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7372..8082
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_site      8097..8551
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     protein_bind    8660..8972
                     /gene="piggyBac 5' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 5'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 5' ITR and flanking 
                     sequence. May contain cryptic promoter activity."
     promoter        complement(9118..9136)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9157..9173)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9181..9197
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9205..9235)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9250..9271
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9559..10147)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10318..11178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11179..11283)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccat
     1621 ggctggacac ctggcttcag acttcgcctt ctcaccccca ccaggtgggg gtgatgggtc
     1681 agcagggctg gagccgggct gggtggatcc tcgaacctgg ctaagcttcc aagggcctcc
     1741 aggtgggcct ggaatcggac caggctcaga ggtattgggg atctccccat gtccgcccgc
     1801 atacgagttc tgcggaggga tggcatactg tggacctcag gttggactgg gcctagtccc
     1861 ccaagttggc gtggagactt tgcagcctga gggccaggca ggagcacgag tggaaagcaa
     1921 ctcagaggga acctcctctg agccctgtgc cgaccgcccc aatgccgtga agttggagaa
     1981 ggtggaacca actcccgagg agtcccagga catgaaagcc ctgcagaagg agctagaaca
     2041 gtttgccaag ctgctgaagc agaagaggat caccttgggg tacacccagg ccgacgtggg
     2101 gctcaccctg ggcgttctct ttggaaaggt gttcagccag accaccatct gtcgcttcga
     2161 ggccttgcag ctcagcctta agaacatgtg taagctgcgg cccctgctgg agaagtgggt
     2221 ggaggaagcc gacaacaatg agaaccttca ggagatatgc aaatcggaga ccctggtgca
     2281 ggcccggaag agaaagcgaa ctagcattga gaaccgtgtg aggtggagtc tggagaccat
     2341 gtttctgaag tgcccgaagc cctccctaca gcagatcact cacatcgcca atcagcttgg
     2401 gctagagaag gatgtggttc gagtatggtt ctgtaaccgg cgccagaagg gcaaaagatc
     2461 aagtattgag tattcccaac gagaagagta tgaggctaca gggacacctt tcccaggggg
     2521 ggctgtatcc tttcctctgc ccccaggtcc ccactttggc accccaggct atggaagccc
     2581 ccacttcacc acactctact cagtcccttt tcctgagggc gaggcctttc cctctgttcc
     2641 cgtcactgct ctgggctctc ccatgcattc aaacgctagc ggcagcggcg ccacgaactt
     2701 ctctctgtta aagcaagcag gagatgttga agaaaacccc gggcctgcat gcatgtataa
     2761 catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg gcggcggcgg
     2821 aggaggcaac gccacggcgg cggcgaccgg cggcaaccag aagaacagcc cggaccgcgt
     2881 caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta agatggccca
     2941 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg agtggaaact
     3001 tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc gcgctctgca
     3061 catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga cgctcatgaa
     3121 gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca gcatggcgag
     3181 cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg acagctacgc
     3241 gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc tgggctaccc
     3301 gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc accgctacga
     3361 cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc
     3421 cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg gctccatggg
     3481 ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt cctcccactc
     3541 cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc tccccggcgc
     3601 cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact accagagcgg
     3661 cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg catgcggctc
     3721 cggcgagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggcccact
     3781 cgagatgagg cagccacctg gcgagtctga catggctgtc agcgacgctc tgctcccgtc
     3841 cttctccacg ttcgcgtccg gcccggcggg aagggagaag acactgcgtc cagcaggtgc
     3901 cccgactaac cgttggcgtg aggaactctc tcacatgaag cgacttcccc cacttcccgg
     3961 ccgcccctac gacctggcgg cgacggtggc cacagacctg gagagtggcg gagctggtgc
     4021 agcttgcagc agtaacaacc cggccctcct agcccggagg gagaccgagg agttcaacga
     4081 cctcctggac ctagacttta tcctttccaa ctcgctaacc caccaggaat cggtggccgc
     4141 caccgtgacc acctcggcgt cagcttcatc ctcgtcttcc ccagcgagca gcggccctgc
     4201 cagcgcgccc tccacctgca gcttcagcta tccgatccgg gccgggggtg acccgggcgt
     4261 ggctgccagc aacacaggtg gagggctcct ctacagccga gaatctgcgc cacctcccac
     4321 ggcccccttc aacctggcgg acatcaatga cgtgagcccc tcgggcggct tcgtggctga
     4381 gctcctgcgg ccggagttgg acccagtata cattccgcca cagcagcctc agccgccagg
     4441 tggcgggctg atgggcaagt ttgtgctgaa ggcgtctctg accacccctg gcagcgagta
     4501 cagcagccct tcggtcatca gtgttagcaa aggaagccca gacggcagcc accccgtggt
     4561 agtggcgccc tacagcggtg gcccgccgcg catgtgcccc aagattaagc aagaggcggt
     4621 cccgtcctgc acggtcagcc ggtccctaga ggcccatttg agcgctggac cccagctcag
     4681 caacggccac cggcccaaca cacacgactt ccccctgggg cggcagctcc ccaccaggac
     4741 tacccctaca ctgagtcccg aggaactgct gaacagcagg gactgtcacc ctggcctgcc
     4801 tcttccccca ggattccatc cccatccggg gcccaactac cctcctttcc tgccagacca
     4861 gatgcagtca caagtcccct ctctccatta tcaagagctc atgccaccgg gttcctgcct
     4921 gccagaggag cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa gaacagccac
     4981 ccacacttgt gactatgcag gctgtggcaa aacctatacc aagagttctc atctcaaggc
     5041 acacctgcga actcacacag gcgagaaacc ttaccactgt gactgggacg gctgtgggtg
     5101 gaaattcgcc cgctccgatg aactgaccag gcactaccgc aaacacacag ggcaccggcc
     5161 ctttcagtgc cagaagtgcg acagggcctt ttccaggtcg gaccaccttg ccttacacat
     5221 gaagaggcac tttctcgagg gctcgggcca gtgtactaat tatgctctct tgaaattggc
     5281 tggagatgtt gagagcaacc caggtcccgg cgcgccactg gatttccttt gggcgttgga
     5341 aaccccgcag acagccacga cgatgcccct caacgtgaac ttcaccaaca ggaactatga
     5401 cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga atttctatca
     5461 ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt
     5521 cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct gctctccatc
     5581 ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg gcggtggcaa
     5641 cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag acatggtgaa
     5701 ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca tcatccagga
     5761 ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc tggcctccta
     5821 ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc
     5881 cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca ttgacccctc
     5941 agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta cctcgtccga
     6001 ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct ccccacgggc
     6061 cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca gcgactctga
     6121 agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga ggcaaacccc
     6181 tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac ctccgcacag
     6241 cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg ccgcaccccc
     6301 ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg gcagggtcct
     6361 gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca cggaggaaaa
     6421 cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc tgaagcgcag
     6481 cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg cccccaaggt
     6541 agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg agcacaagct
     6601 cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca aactcgaaca
     6661 gcttcgaaac tctggtgcat aaggcgcgcc gaattcgcgg ccgcactcga gatatctaga
     6721 cccagctttc ttgtacaaag tggtgatcta gctaaaacgc ggcctcgaat cgaattccgc
     6781 ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     6841 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     6901 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6961 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     7021 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     7081 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     7141 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     7201 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     7261 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
     7321 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
     7381 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     7441 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     7501 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     7561 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     7621 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     7681 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     7741 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     7801 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     7861 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     7921 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     7981 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     8041 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgatcttttt
     8101 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     8161 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     8221 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     8281 gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta
     8341 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     8401 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     8461 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     8521 tcttctctta tgaagatccc tcgacctgca ggaattcgat atcaagctct agccaacaag
     8581 ctcgtcatcg ctttgcagaa gagcagagag gatatgctca tcgtctaaag aactacccat
     8641 tttattatat attagtcacg atatctataa caagaaaata tatatataat aagttatcac
     8701 gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt
     8761 aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac
     8821 ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat
     8881 gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt
     8941 caattttacg cagactatct ttctagggtt aaaaaagatt tgcgctttac tcgacctaaa
     9001 ctttaaacac gtcatagaat cttcgtttga caaaaaccac attgtggcca agctgtgtga
     9061 cgcgacgcgc gctaaagaat ggcaaaccaa gtcgcgcgag gtacccagct tttgttccct
     9121 ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9181 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9241 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9361 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9961 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    10021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10621 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10921 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11161 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11221 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11281 gcgcacattt ccccgaaaag tgccacctg
//
