LOCUS       Exported               11404 bp ds-DNA     circular SYN 27-3月-2015
DEFINITION  KW252_PB-TAC-OK+9MS; piggyBac transposon system for dox-inducible 
            iPS cell generation (OK+9MS cassette with mCherry reporter).
ACCESSION   .
VERSION     .
KEYWORDS    KW252_PB-TAC-OK+9MS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K.
  TITLE     KLF4 N-Terminal Variance Modulates Induced Reprogramming to 
            Pluripotency.
  JOURNAL   Stem Cell Reports 2015.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Knut Woltjen
  TITLE     Direct Submission
  JOURNAL   Exported 2015年3月30日 from SnapGene 2.6.2
            http://www.snapgene.com
COMMENT     Requires piggyBac transposase for genomic integration.
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=M13 origin
                     /note="M13 origin"
     rep_origin      13..453
                     /label=F1 ori
                     /note="F1 ori"
     CDS             complement(463..531)
                     /label=LacZ alpha
                     /note="LacZ alpha"
     primer_bind     603..619
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(707..941)
                     /gene="piggyBac 3' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 3'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 3' ITR and flanking 
                     sequence."
     protein_bind    1145..1163
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1431..1530
                     /label=mCMV promoter
                     /note="mCMV promoter"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1645..6759
                     /codon_start=1
                     /note="OK+9MS cassette"
                     /translation="MAGHLASDFAFSPPPGGGDGSAGLEPGWVDSRTWLSFQGPPGGPG
                     IGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG
                     TSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLT
                     LGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQA
                     RKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRS
                     SIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSV
                     PVTALGSPMHSNGDLKIVAPVKQTLNFDLLKLAGDVESNPGPMRQPPGESDMAVSDALL
                     PSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRLPPLPGRPYDLAATVATDLESGG
                     AGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSPAS
                     SGPASAPSTCSFSYPIRAGGDPGVAASNTGGGLLYSRESAPPPTAPFNLADINDVSPSG
                     GFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLTTPGSEYSSPSVISVSKGSPD
                     GSHPVVVAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLSAGPQLSNGHRPNTHDFPLG
                     RQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPSLHYQE
                     LMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPY
                     HCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFLEGSGQ
                     CTNYALLKLAGDVESNPGPGAPLDFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPY
                     FICDEEENFYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSF
                     SPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFS
                     AAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYP
                     LNDSSSPKSCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQED
                     EEEIDVVSVEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRK
                     DYPAAKRAKLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFA
                     LRDQIPELENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLR
                     NSGAGDLKIVAPVKQTLNFDLLKLAGDVESNPGPLMNNSPFTMYNMMETELKPPGPQQA
                     SGGGGGGGNATAAATGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKR
                     LGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLA
                     PGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQEQLGYPQHPGLNAHGAA
                     QMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSS
                     PPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMAQHYQSGPVPGTAING
                     TLPLSHM"
     CDS             1648..2700
                     /codon_start=1
                     /note="Oct3/4"
                     /note="REFSEQ:NM_013633.3"
                     /translation="AGHLASDFAFSPPPGGGDGSAGLEPGWVDSRTWLSFQGPPGGPGI
                     GPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGT
                     SSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTL
                     GVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQAR
                     KRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSS
                     IEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVP
                     VTALGSPMHSN"
     variation       1735
                     /note="rs8252753"
                     /note="Missense variant"
                     /db_xref="dbSNP:<html><body>rs8252753</body></html>"
     CDS             2725..2790
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /note="F2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     variation       2791..2817
                     /note="+9aa"
                     /note="GENBANK:U20344.1"
     CDS             2794..4239
                     /codon_start=1
                     /note="Klf4"
                     /note="REFSEQ:NM_010637.3;Klf4-S"
                     /translation="RQPPGESDMAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWRE
                     ELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDF
                     ILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAASNT
                     GGGLLYSRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLM
                     GKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSC
                     TVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLP
                     PGFHPHPGPNYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATH
                     TCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRP
                     FQCQKCDRAFSRSDHLALHMKRHF"
     variation       3189
                     /note="rs16802583"
                     /note="Synonymous variant"
                     /db_xref="dbSNP:<html><body>rs16802583</body></html>"
     variation       4185
                     /note="rs32168644"
                     /note="Synonymous variant"
                     /db_xref="dbSNP:<html><body>rs32168644</body></html>"
     CDS             4255..4314
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /note="E2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4327..5685
                     /codon_start=1
                     /note="cMyc"
                     /note="REFSEQ:NM_010849.4;isoform a;non-canonical start"
                     /translation="DFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPYFICDEEENF
                     YHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGG
                     GGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEK
                     LASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKS
                     CTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSV
                     EKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAK
                     LDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELE
                     NNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA"
     CDS             5710..5775
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /note="F2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             5803..6756
                     /codon_start=1
                     /note="Sox2"
                     /note="REFSEQ:NM_011443.3"
                     /translation="YNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKRP
                     MNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMK
                     EHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAH
                     MNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSP
                     TYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPG
                     AEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM"
     protein_bind    complement(6815..6839)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6881..7466
                     /note="IRES2"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7467..8177
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_site      8192..8646
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     protein_bind    8755..9067
                     /gene="piggyBac 5' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 5'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 5' ITR and flanking 
                     sequence. May contain cryptic promoter activity."
     promoter        complement(9213..9231)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9252..9268)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9276..9292
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9300..9330)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9345..9366
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9654..10242)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10413..11273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11274..11378)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccct
     1621 taaaaagcag gctccacctt ccccatggct ggacacctgg cttcagactt cgccttctca
     1681 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggattctcga
     1741 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta
     1801 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga
     1861 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc
     1921 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac
     1981 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg
     2041 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc
     2101 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc
     2161 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag
     2221 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag
     2281 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac
     2341 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag
     2401 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt
     2461 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag
     2521 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac
     2581 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct
     2641 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac
     2701 ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg
     2761 gctggggatg tagaaagcaa tccaggtcca atgaggcagc cacctggcga gtctgacatg
     2821 gctgtcagcg acgctctgct cccgtccttc tccacgttcg cgtccggccc ggcgggaagg
     2881 gagaagacac tgcgtccagc aggtgccccg actaaccgtt ggcgtgagga actctctcac
     2941 atgaagcgac ttcccccact tcccggccgc ccctacgacc tggcggcgac ggtggccaca
     3001 gacctggaga gtggcggagc tggtgcagct tgcagcagta acaacccggc cctcctagcc
     3061 cggagggaga ccgaggagtt caacgacctc ctggacctag actttatcct ttccaactcg
     3121 ctaacccacc aggaatcggt ggccgccacc gtgaccacct cggcgtcagc ttcatcctcg
     3181 tcttccccag cgagcagcgg ccctgccagc gcgccctcca cctgcagctt cagctatccg
     3241 atccgggccg ggggtgaccc gggcgtggct gccagcaaca caggtggagg gctcctctac
     3301 agccgagaat ctgcgccacc tcccacggcc cccttcaacc tggcggacat caatgacgtg
     3361 agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg agttggaccc agtatacatt
     3421 ccgccacagc agcctcagcc gccaggtggc gggctgatgg gcaagtttgt gctgaaggcg
     3481 tctctgacca cccctggcag cgagtacagc agcccttcgg tcatcagtgt tagcaaagga
     3541 agcccagacg gcagccaccc cgtggtagtg gcgccctaca gcggtggccc gccgcgcatg
     3601 tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg tcagccggtc cctagaggcc
     3661 catttgagcg ctggacccca gctcagcaac ggccaccggc ccaacacaca cgacttcccc
     3721 ctggggcggc agctccccac caggactacc cctacactga gtcccgagga actgctgaac
     3781 agcagggact gtcaccctgg cctgcctctt cccccaggat tccatcccca tccggggccc
     3841 aactaccctc ctttcctgcc agaccagatg cagtcacaag tcccctctct ccattatcaa
     3901 gagctcatgc caccgggttc ctgcctgcca gaggagccca agccaaagag gggaagaagg
     3961 tcgtggcccc ggaaaagaac agccacccac acttgtgact atgcaggctg tggcaaaacc
     4021 tataccaaga gttctcatct caaggcacac ctgcgaactc acacaggcga gaaaccttac
     4081 cactgtgact gggacggctg tgggtggaaa ttcgcccgct ccgatgaact gaccaggcac
     4141 taccgcaaac acacagggca ccggcccttt cagtgccaga agtgcgacag ggccttttcc
     4201 aggtcggacc accttgcctt acacatgaag aggcactttc tcgagggctc gggccagtgt
     4261 actaattatg ctctcttgaa attggctgga gatgttgaga gcaacccagg tcccggcgcg
     4321 ccactggatt tcctttgggc gttggaaacc ccgcagacag ccacgacgat gcccctcaac
     4381 gtgaacttca ccaacaggaa ctatgacctc gactacgact ccgtacagcc ctatttcatc
     4441 tgcgacgagg aagagaattt ctatcaccag caacagcaga gcgagctgca gccgcccgcg
     4501 cccagtgagg atatctggaa gaaattcgag ctgcttccca ccccgcccct gtccccgagc
     4561 cgccgctccg ggctctgctc tccatcctat gttgcggtcg ctacgtcctt ctccccaagg
     4621 gaagacgatg acggcggcgg tggcaacttc tccaccgccg atcagctgga gatgatgacc
     4681 gagttacttg gaggagacat ggtgaaccag agcttcatct gcgatcctga cgacgagacc
     4741 ttcatcaaga acatcatcat ccaggactgt atgtggagcg gtttctcagc cgctgccaag
     4801 ctggtctcgg agaagctggc ctcctaccag gctgcgcgca aagacagcac cagcctgagc
     4861 cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt acctgcagga cctcaccgcc
     4921 gccgcgtccg agtgcattga cccctcagtg gtctttccct acccgctcaa cgacagcagc
     4981 tcgcccaaat cctgtacctc gtccgattcc acggccttct ctccttcctc ggactcgctg
     5041 ctgtcctccg agtcctcccc acgggccagc cctgagcccc tagtgctgca tgaggagaca
     5101 ccgcccacca ccagcagcga ctctgaagaa gagcaagaag atgaggaaga aattgatgtg
     5161 gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg agtcgggctc atctccatcc
     5221 cgaggccaca gcaaacctcc gcacagccca ctggtcctca agaggtgcca cgtctccact
     5281 caccagcaca actacgccgc acccccctcc acaaggaagg actatccagc tgccaagagg
     5341 gccaagttgg acagtggcag ggtcctgaag cagatcagca acaaccgcaa gtgctccagc
     5401 cccaggtcct cagacacgga ggaaaacgac aagaggcgga cacacaacgt cttggaacgt
     5461 cagaggagga acgagctgaa gcgcagcttt tttgccctgc gtgaccagat ccctgaattg
     5521 gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa aagccaccgc ctacatcctg
     5581 tccattcaag cagacgagca caagctcacc tctgaaaagg acttattgag gaaacgacga
     5641 gaacagttga aacacaaact cgaacagctt cgaaactctg gtgcagggga tctcaaaatt
     5701 gtcgctcctg tcaaacaaac tcttaacttt gatttactca aactggctgg ggatgtagaa
     5761 agcaatccag gtccactcat gaacaattcg cccttcacca tgtataacat gatggagacg
     5821 gagctgaagc cgccgggccc gcagcaagct tcggggggcg gcggcggagg aggcaacgcc
     5881 acggcggcgg cgaccggcgg caaccagaag aacagcccgg accgcgtcaa gaggcccatg
     5941 aacgccttca tggtatggtc ccgggggcag cggcgtaaga tggcccagga gaaccccaag
     6001 atgcacaact cggagatcag caagcgcctg ggcgcggagt ggaaactttt gtccgagacc
     6061 gagaagcggc cgttcatcga cgaggccaag cggctgcgcg ctctgcacat gaaggagcac
     6121 ccggattata aataccggcc gcggcggaaa accaagacgc tcatgaagaa ggataagtac
     6181 acgcttcccg gaggcttgct ggcccccggc gggaacagca tggcgagcgg ggttggggtg
     6241 ggcgccggcc tgggtgcggg cgtgaaccag cgcatggaca gctacgcgca catgaacggc
     6301 tggagcaacg gcagctacag catgatgcag gagcagctgg gctacccgca gcacccgggc
     6361 ctcaacgctc acggcgcggc acagatgcaa ccgatgcacc gctacgacgt cagcgccctg
     6421 cagtacaact ccatgaccag ctcgcagacc tacatgaacg gctcgcccac ctacagcatg
     6481 tcctactcgc agcagggcac ccccggtatg gcgctgggct ccatgggctc tgtggtcaag
     6541 tccgaggcca gctccagccc ccccgtggtt acctcttcct cccactccag ggcgccctgc
     6601 caggccgggg acctccggga catgatcagc atgtacctcc ccggcgccga ggtgccggag
     6661 cccgctgcgc ccagtagact gcacatggcc cagcactacc agagcggccc ggtgcccggc
     6721 acggccatta acggcacact gcccctgtcg cacatgtgag ggctggaagg gcgaattgcc
     6781 tgcaggaatt cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg
     6841 atctagctaa aacgcggcct cgaatcgaat tccgcccccc cccctctccc tccccccccc
     6901 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     6961 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     7021 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     7081 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     7141 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     7201 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     7261 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     7321 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     7381 agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     7441 aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggaggat aacatggcca
     7501 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     7561 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     7621 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     7681 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     7741 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7801 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     7861 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     7921 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     7981 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     8041 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     8101 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     8161 acgagctgta caagtaaagc ggccgcgatc tttttccctc tgccaaaaat tatggggaca
     8221 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     8281 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     8341 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc
     8401 catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     8461 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     8521 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     8581 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgac
     8641 ctgcaggaat tcgatatcaa gctctagcca acaagctcgt catcgctttg cagaagagca
     8701 gagaggatat gctcatcgtc taaagaacta cccattttat tatatattag tcacgatatc
     8761 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
     8821 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga
     8881 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca
     8941 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt
     9001 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta
     9061 gggttaaaaa agatttgcgc tttactcgac ctaaacttta aacacgtcat agaatcttcg
     9121 tttgacaaaa accacattgt ggccaagctg tgtgacgcga cgcgcgctaa agaatggcaa
     9181 accaagtcgc gcgaggtacc cagcttttgt tccctttagt gagggttaat tccgagcttg
     9241 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9601 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9661 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9721 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9781 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9901 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9961 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10021 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10081 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10141 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10261 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10321 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10381 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10501 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10621 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10741 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10801 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10861 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10921 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10981 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11041 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11101 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11161 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11221 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11281 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11341 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11401 cctg
//
