LOCUS Exported 11404 bp ds-DNA circular SYN 27-3月-2015 DEFINITION KW252_PB-TAC-OK+9MS; piggyBac transposon system for dox-inducible iPS cell generation (OK+9MS cassette with mCherry reporter). ACCESSION . VERSION . KEYWORDS KW252_PB-TAC-OK+9MS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11404) AUTHORS Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, Yamamoto T, Yamanaka S, Woltjen K. TITLE KLF4 N-Terminal Variance Modulates Induced Reprogramming to Pluripotency. JOURNAL Stem Cell Reports 2015. PUBMED 25772473 REFERENCE 2 (bases 1 to 11404) AUTHORS Knut Woltjen TITLE Direct Submission JOURNAL Exported 2015年3月30日 from SnapGene 2.6.2 http://www.snapgene.com COMMENT Requires piggyBac transposase for genomic integration. FEATURES Location/Qualifiers source 1..11404 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(3..458) /direction=LEFT /label=M13 origin /note="M13 origin" rep_origin 13..453 /label=F1 ori /note="F1 ori" CDS complement(463..531) /label=LacZ alpha /note="LacZ alpha" primer_bind 603..619 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(707..941) /gene="piggyBac 3' inverted terminal repeat" /bound_moiety="piggyBac Transposase" /note="PB 3'ITR" /note="Recombination site flanking the pigyBac transposon. Terminates in TTAA. Contains minimal 3' ITR and flanking sequence." protein_bind 1145..1163 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1182..1200 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1219..1237 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1256..1274 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1293..1311 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1330..1348 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1367..1385 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 1404..1422 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 1431..1530 /label=mCMV promoter /note="mCMV promoter" protein_bind 1548..1572 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB1" /note="recombination site for the Gateway(R) BP reaction" CDS 1645..6759 /codon_start=1 /note="OK+9MS cassette" /translation="MAGHLASDFAFSPPPGGGDGSAGLEPGWVDSRTWLSFQGPPGGPG IGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG TSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLT LGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQA RKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRS SIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSV PVTALGSPMHSNGDLKIVAPVKQTLNFDLLKLAGDVESNPGPMRQPPGESDMAVSDALL PSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRLPPLPGRPYDLAATVATDLESGG AGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTSASASSSSSPAS SGPASAPSTCSFSYPIRAGGDPGVAASNTGGGLLYSRESAPPPTAPFNLADINDVSPSG GFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLTTPGSEYSSPSVISVSKGSPD GSHPVVVAPYSGGPPRMCPKIKQEAVPSCTVSRSLEAHLSAGPQLSNGHRPNTHDFPLG RQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPSLHYQE LMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPY HCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFLEGSGQ CTNYALLKLAGDVESNPGPGAPLDFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPY FICDEEENFYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSF SPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFS AAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYP LNDSSSPKSCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQED EEEIDVVSVEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRK DYPAAKRAKLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFA LRDQIPELENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLR NSGAGDLKIVAPVKQTLNFDLLKLAGDVESNPGPLMNNSPFTMYNMMETELKPPGPQQA SGGGGGGGNATAAATGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKR LGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLA PGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQEQLGYPQHPGLNAHGAA QMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSS PPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMAQHYQSGPVPGTAING TLPLSHM" CDS 1648..2700 /codon_start=1 /note="Oct3/4" /note="REFSEQ:NM_013633.3" /translation="AGHLASDFAFSPPPGGGDGSAGLEPGWVDSRTWLSFQGPPGGPGI GPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGT SSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTL GVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQAR KRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSS IEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVP VTALGSPMHSN" variation 1735 /note="rs8252753" /note="Missense variant" /db_xref="dbSNP:rs8252753" CDS 2725..2790 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /note="F2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" variation 2791..2817 /note="+9aa" /note="GENBANK:U20344.1" CDS 2794..4239 /codon_start=1 /note="Klf4" /note="REFSEQ:NM_010637.3;Klf4-S" /translation="RQPPGESDMAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWRE ELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDF ILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAASNT GGGLLYSRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLM GKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSC TVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLP PGFHPHPGPNYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATH TCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRP FQCQKCDRAFSRSDHLALHMKRHF" variation 3189 /note="rs16802583" /note="Synonymous variant" /db_xref="dbSNP:rs16802583" variation 4185 /note="rs32168644" /note="Synonymous variant" /db_xref="dbSNP:rs32168644" CDS 4255..4314 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /note="E2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" CDS 4327..5685 /codon_start=1 /note="cMyc" /note="REFSEQ:NM_010849.4;isoform a;non-canonical start" /translation="DFLWALETPQTATTMPLNVNFTNRNYDLDYDSVQPYFICDEEENF YHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGG GGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEK LASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKS CTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSV EKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAK LDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELE NNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA" CDS 5710..5775 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /note="F2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" CDS 5803..6756 /codon_start=1 /note="Sox2" /note="REFSEQ:NM_011443.3" /translation="YNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKRP MNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMK EHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAH MNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSP TYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPG AEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM" protein_bind complement(6815..6839) /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB2" /note="recombination site for the Gateway(R) BP reaction" CDS 6881..7466 /note="IRES2" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 7467..8177 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mCherry" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" polyA_site 8192..8646 /note="beta-globin poly(A) signal" /note="rabbit beta-globin polyadenylation signal" protein_bind 8755..9067 /gene="piggyBac 5' inverted terminal repeat" /bound_moiety="piggyBac Transposase" /note="PB 5'ITR" /note="Recombination site flanking the pigyBac transposon. Terminates in TTAA. Contains minimal 5' ITR and flanking sequence. May contain cryptic promoter activity." promoter complement(9213..9231) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9252..9268) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 9276..9292 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9300..9330) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 9345..9366 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(9654..10242) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10413..11273) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(11274..11378) /gene="bla" /note="AmpR promoter" ORIGIN 1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg 661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct 961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt 1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac 1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca 1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga 1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct 1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac 1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat 1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag 1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca 1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcgccct 1621 taaaaagcag gctccacctt ccccatggct ggacacctgg cttcagactt cgccttctca 1681 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggattctcga 1741 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta 1801 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga 1861 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc 1921 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac 1981 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg 2041 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc 2101 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc 2161 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag 2221 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag 2281 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac 2341 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag 2401 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt 2461 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag 2521 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac 2581 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct 2641 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac 2701 ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg 2761 gctggggatg tagaaagcaa tccaggtcca atgaggcagc cacctggcga gtctgacatg 2821 gctgtcagcg acgctctgct cccgtccttc tccacgttcg cgtccggccc ggcgggaagg 2881 gagaagacac tgcgtccagc aggtgccccg actaaccgtt ggcgtgagga actctctcac 2941 atgaagcgac ttcccccact tcccggccgc ccctacgacc tggcggcgac ggtggccaca 3001 gacctggaga gtggcggagc tggtgcagct tgcagcagta acaacccggc cctcctagcc 3061 cggagggaga ccgaggagtt caacgacctc ctggacctag actttatcct ttccaactcg 3121 ctaacccacc aggaatcggt ggccgccacc gtgaccacct cggcgtcagc ttcatcctcg 3181 tcttccccag cgagcagcgg ccctgccagc gcgccctcca cctgcagctt cagctatccg 3241 atccgggccg ggggtgaccc gggcgtggct gccagcaaca caggtggagg gctcctctac 3301 agccgagaat ctgcgccacc tcccacggcc cccttcaacc tggcggacat caatgacgtg 3361 agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg agttggaccc agtatacatt 3421 ccgccacagc agcctcagcc gccaggtggc gggctgatgg gcaagtttgt gctgaaggcg 3481 tctctgacca cccctggcag cgagtacagc agcccttcgg tcatcagtgt tagcaaagga 3541 agcccagacg gcagccaccc cgtggtagtg gcgccctaca gcggtggccc gccgcgcatg 3601 tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg tcagccggtc cctagaggcc 3661 catttgagcg ctggacccca gctcagcaac ggccaccggc ccaacacaca cgacttcccc 3721 ctggggcggc agctccccac caggactacc cctacactga gtcccgagga actgctgaac 3781 agcagggact gtcaccctgg cctgcctctt cccccaggat tccatcccca tccggggccc 3841 aactaccctc ctttcctgcc agaccagatg cagtcacaag tcccctctct ccattatcaa 3901 gagctcatgc caccgggttc ctgcctgcca gaggagccca agccaaagag gggaagaagg 3961 tcgtggcccc ggaaaagaac agccacccac acttgtgact atgcaggctg tggcaaaacc 4021 tataccaaga gttctcatct caaggcacac ctgcgaactc acacaggcga gaaaccttac 4081 cactgtgact gggacggctg tgggtggaaa ttcgcccgct ccgatgaact gaccaggcac 4141 taccgcaaac acacagggca ccggcccttt cagtgccaga agtgcgacag ggccttttcc 4201 aggtcggacc accttgcctt acacatgaag aggcactttc tcgagggctc gggccagtgt 4261 actaattatg ctctcttgaa attggctgga gatgttgaga gcaacccagg tcccggcgcg 4321 ccactggatt tcctttgggc gttggaaacc ccgcagacag ccacgacgat gcccctcaac 4381 gtgaacttca ccaacaggaa ctatgacctc gactacgact ccgtacagcc ctatttcatc 4441 tgcgacgagg aagagaattt ctatcaccag caacagcaga gcgagctgca gccgcccgcg 4501 cccagtgagg atatctggaa gaaattcgag ctgcttccca ccccgcccct gtccccgagc 4561 cgccgctccg ggctctgctc tccatcctat gttgcggtcg ctacgtcctt ctccccaagg 4621 gaagacgatg acggcggcgg tggcaacttc tccaccgccg atcagctgga gatgatgacc 4681 gagttacttg gaggagacat ggtgaaccag agcttcatct gcgatcctga cgacgagacc 4741 ttcatcaaga acatcatcat ccaggactgt atgtggagcg gtttctcagc cgctgccaag 4801 ctggtctcgg agaagctggc ctcctaccag gctgcgcgca aagacagcac cagcctgagc 4861 cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt acctgcagga cctcaccgcc 4921 gccgcgtccg agtgcattga cccctcagtg gtctttccct acccgctcaa cgacagcagc 4981 tcgcccaaat cctgtacctc gtccgattcc acggccttct ctccttcctc ggactcgctg 5041 ctgtcctccg agtcctcccc acgggccagc cctgagcccc tagtgctgca tgaggagaca 5101 ccgcccacca ccagcagcga ctctgaagaa gagcaagaag atgaggaaga aattgatgtg 5161 gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg agtcgggctc atctccatcc 5221 cgaggccaca gcaaacctcc gcacagccca ctggtcctca agaggtgcca cgtctccact 5281 caccagcaca actacgccgc acccccctcc acaaggaagg actatccagc tgccaagagg 5341 gccaagttgg acagtggcag ggtcctgaag cagatcagca acaaccgcaa gtgctccagc 5401 cccaggtcct cagacacgga ggaaaacgac aagaggcgga cacacaacgt cttggaacgt 5461 cagaggagga acgagctgaa gcgcagcttt tttgccctgc gtgaccagat ccctgaattg 5521 gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa aagccaccgc ctacatcctg 5581 tccattcaag cagacgagca caagctcacc tctgaaaagg acttattgag gaaacgacga 5641 gaacagttga aacacaaact cgaacagctt cgaaactctg gtgcagggga tctcaaaatt 5701 gtcgctcctg tcaaacaaac tcttaacttt gatttactca aactggctgg ggatgtagaa 5761 agcaatccag gtccactcat gaacaattcg cccttcacca tgtataacat gatggagacg 5821 gagctgaagc cgccgggccc gcagcaagct tcggggggcg gcggcggagg aggcaacgcc 5881 acggcggcgg cgaccggcgg caaccagaag aacagcccgg accgcgtcaa gaggcccatg 5941 aacgccttca tggtatggtc ccgggggcag cggcgtaaga tggcccagga gaaccccaag 6001 atgcacaact cggagatcag caagcgcctg ggcgcggagt ggaaactttt gtccgagacc 6061 gagaagcggc cgttcatcga cgaggccaag cggctgcgcg ctctgcacat gaaggagcac 6121 ccggattata aataccggcc gcggcggaaa accaagacgc tcatgaagaa ggataagtac 6181 acgcttcccg gaggcttgct ggcccccggc gggaacagca tggcgagcgg ggttggggtg 6241 ggcgccggcc tgggtgcggg cgtgaaccag cgcatggaca gctacgcgca catgaacggc 6301 tggagcaacg gcagctacag catgatgcag gagcagctgg gctacccgca gcacccgggc 6361 ctcaacgctc acggcgcggc acagatgcaa ccgatgcacc gctacgacgt cagcgccctg 6421 cagtacaact ccatgaccag ctcgcagacc tacatgaacg gctcgcccac ctacagcatg 6481 tcctactcgc agcagggcac ccccggtatg gcgctgggct ccatgggctc tgtggtcaag 6541 tccgaggcca gctccagccc ccccgtggtt acctcttcct cccactccag ggcgccctgc 6601 caggccgggg acctccggga catgatcagc atgtacctcc ccggcgccga ggtgccggag 6661 cccgctgcgc ccagtagact gcacatggcc cagcactacc agagcggccc ggtgcccggc 6721 acggccatta acggcacact gcccctgtcg cacatgtgag ggctggaagg gcgaattgcc 6781 tgcaggaatt cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg 6841 atctagctaa aacgcggcct cgaatcgaat tccgcccccc cccctctccc tccccccccc 6901 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat 6961 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct 7021 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg 7081 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc 7141 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg 7201 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg 7261 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga 7321 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt 7381 agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa 7441 aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggaggat aacatggcca 7501 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 7561 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 7621 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 7681 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 7741 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 7801 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca 7861 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct 7921 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 7981 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 8041 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg 8101 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg 8161 acgagctgta caagtaaagc ggccgcgatc tttttccctc tgccaaaaat tatggggaca 8221 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa 8281 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt 8341 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc 8401 catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat 8461 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg 8521 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc 8581 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgac 8641 ctgcaggaat tcgatatcaa gctctagcca acaagctcgt catcgctttg cagaagagca 8701 gagaggatat gctcatcgtc taaagaacta cccattttat tatatattag tcacgatatc 8761 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata 8821 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga 8881 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca 8941 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt 9001 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta 9061 gggttaaaaa agatttgcgc tttactcgac ctaaacttta aacacgtcat agaatcttcg 9121 tttgacaaaa accacattgt ggccaagctg tgtgacgcga cgcgcgctaa agaatggcaa 9181 accaagtcgc gcgaggtacc cagcttttgt tccctttagt gagggttaat tccgagcttg 9241 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 9301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 9361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 9421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 9481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 9541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 9601 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 9661 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 9721 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 9781 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 9841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 9901 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 9961 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 10021 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 10081 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 10141 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 10201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 10261 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 10321 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 10381 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 10441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 10501 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 10561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 10621 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 10681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 10741 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 10801 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 10861 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 10921 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 10981 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 11041 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 11101 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 11161 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 11221 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 11281 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 11341 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 11401 cctg //