LOCUS       Exported               11904 bp ds-DNA     circular SYN 27-3月-2015
DEFINITION  KW505_PB-TAC-EB-C5; piggyBac transposon system for dox-inducible iPS
            cell generation (EB-C5 cassette with mCherry reporter).
ACCESSION   .
VERSION     .
KEYWORDS    KW505_PB-TAC-EB-C5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K.
  TITLE     KLF4 N-Terminal Variance Modulates Induced Reprogramming to 
            Pluripotency.
  JOURNAL   Stem Cell Reports 2015.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Knut Woltjen
  TITLE     Direct Submission
  JOURNAL   Exported 2015年3月30日 from SnapGene 2.6.2
            http://www.snapgene.com
COMMENT     Requires piggyBac transposase for genomic integration.
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=M13 origin
                     /note="M13 origin"
     rep_origin      13..453
                     /label=F1 ori
                     /note="F1 ori"
     CDS             complement(463..531)
                     /label=LacZ alpha
                     /note="LacZ alpha"
     primer_bind     603..619
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(707..941)
                     /gene="piggyBac 3' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 3'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 3' ITR and flanking 
                     sequence."
     protein_bind    1145..1163
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    1431..1530
                     /label=mCMV promoter
                     /note="mCMV promoter"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1625..7300
                     /codon_start=1
                     /note="EB-C5 cassette"
                     /translation="MAGHLASDFAFSPPPGGGDGSAGLEPGWVVSRTWLSFQGPPGGPG
                     IGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG
                     TSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLT
                     LGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQA
                     RKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRS
                     SIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSV
                     PVTALGSPMHSNGGGSGGEGRGSLLTCGDVEENPGPMYNMMETELKPPGPQQASGGGGG
                     GGNATAAATGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWK
                     LLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSM
                     ASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMH
                     RYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTS
                     SSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSH
                     MGGGSGGEGRGSLLTCGDVEENPGPMAVSDALLPSFSTFASGPAGREKTLRPAGAPTNR
                     WREELSHMKRLPPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLD
                     LDFILSNSLTHQESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAA
                     RNTGGGLLYSRESAPPPTAPFNLGDINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGG
                     GLMGKFVLKASLTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAV
                     PSCTVSRSLEAHLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGL
                     PLPPGFHPHPGANYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRT
                     ATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTG
                     HRPFQCQKCDRAFSRSDHLALHMKRHFGSGKQLLNFDLLKLAGDVESNPGPVEMPLNVN
                     FTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSR
                     RSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDET
                     FIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLT
                     AAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHE
                     ETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCH
                     VSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHN
                     VLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSIQADEHKLTSEKDL
                     LRKRREQLKHKLEQLRNSGAGGGSGGEGRGSLLTCGDVEENPGPMGSVSNQQFAGGCAK
                     AAEKAPEEAPPDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFV
                     HQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKNMQKRR
                     SKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSINHMVASCPLKAQQGPSSQGKPAYFR
                     EEEEEIHSPALLPEAQN"
     CDS             1628..2680
                     /codon_start=1
                     /note="Oct3/4"
                     /note="REFSEQ:NM_013633.3"
                     /translation="AGHLASDFAFSPPPGGGDGSAGLEPGWVVSRTWLSFQGPPGGPGI
                     GPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGT
                     SSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTL
                     GVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQAR
                     KRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSS
                     IEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVP
                     VTALGSPMHSN"
     variation       1713
                     /note="A>T"
                     /note="Missense variant;Asp>Val"
     variation       1715
                     /note="rs8252753"
                     /note="Missense variant"
                     /db_xref="dbSNP:<html><body>rs8252753</body></html>"
     CDS             2699..2752
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2756..3709
                     /codon_start=1
                     /note="Sox2"
                     /note="REFSEQ:NM_011443.3"
                     /translation="YNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKRP
                     MNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMK
                     EHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAH
                     MNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSP
                     TYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPG
                     AEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM"
     CDS             3728..3781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3782..5203
                     /codon_start=1
                     /note="Klf4"
                     /note="REFSEQ:NM_010637.3;Klf4-S"
                     /translation="MAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRL
                     PPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTH
                     QESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAARNTGGGLLYSR
                     ESAPPPTAPFNLGDINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKAS
                     LTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSCTVSRSLEA
                     HLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPG
                     ANYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCG
                     KTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDR
                     AFSRSDHLALHMKRHF"
     variation       4153
                     /note="rs16802583"
                     /note="Synonymous variant"
                     /db_xref="dbSNP:<html><body>rs16802583</body></html>"
     variation       4240
                     /note="C>A"
                     /note="Ser>Arg"
     variation       4308
                     /note="C>G"
                     /note="Synonymous variant"
     variation       4414
                     /note="C>G"
                     /note="Synonymous variant"
     variation       4802
                     /note="C>G"
                     /note="Pro>Ser"
     variation       5149
                     /note="rs32168644"
                     /note="Synonymous variant"
                     /db_xref="dbSNP:<html><body>rs32168644</body></html>"
     CDS             5213..5275
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein (modified Thr/Leu)"
                     /note="F2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="KQLLNFDLLKLAGDVESNPGP"
     CDS             5285..6598
                     /codon_start=1
                     /note="cMyc"
                     /note="REFSEQ:NM_010849.4;isoform b;canonical start"
                     /translation="PLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAPS
                     EDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSLS
                     PARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSSDS
                     LLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESGSS
                     PSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNRK
                     CSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT
                     AYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA"
     CDS             6617..6670
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6671..7297
                     /codon_start=1
                     /note="Lin28"
                     /translation="MGSVSNQQFAGGCAKAAEKAPEEAPPDAARAADEPQLLHGAGICK
                     WFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGL
                     ESIRVTGPGGVFCIGSERRPKGKNMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHF
                     CQSINHMVASCPLKAQQGPSSQGKPAYFREEEEEIHSPALLPEAQN"
     protein_bind    complement(7315..7339)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7381..7966
                     /note="IRES2"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7967..8677
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_site      8692..9146
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     protein_bind    9255..9567
                     /gene="piggyBac 5' inverted terminal repeat"
                     /bound_moiety="piggyBac Transposase"
                     /note="PB 5'ITR"
                     /note="Recombination site flanking the pigyBac transposon. 
                     Terminates in TTAA. Contains minimal 5' ITR and flanking 
                     sequence. May contain cryptic promoter activity."
     promoter        complement(9713..9731)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9752..9768)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9776..9792
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9800..9830)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9845..9866
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10154..10742)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10913..11773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11774..11878)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcagatc
     1621 caccatggct ggacacctgg cttcagactt cgccttctca cccccaccag gtgggggtga
     1681 tgggtcagca gggctggagc cgggctgggt ggtttctcga acctggctaa gcttccaagg
     1741 gcctccaggt gggcctggaa tcggaccagg ctcagaggta ttggggatct ccccatgtcc
     1801 gcccgcatac gagttctgcg gagggatggc atactgtgga cctcaggttg gactgggcct
     1861 agtcccccaa gttggcgtgg agactttgca gcctgagggc caggcaggag cacgagtgga
     1921 aagcaactca gagggaacct cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt
     1981 ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg aaagccctgc agaaggagct
     2041 agaacagttt gccaagctgc tgaagcagaa gaggatcacc ttggggtaca cccaggccga
     2101 cgtggggctc accctgggcg ttctctttgg aaaggtgttc agccagacca ccatctgtcg
     2161 cttcgaggcc ttgcagctca gccttaagaa catgtgtaag ctgcggcccc tgctggagaa
     2221 gtgggtggag gaagccgaca acaatgagaa ccttcaggag atatgcaaat cggagaccct
     2281 ggtgcaggcc cggaagagaa agcgaactag cattgagaac cgtgtgaggt ggagtctgga
     2341 gaccatgttt ctgaagtgcc cgaagccctc cctacagcag atcactcaca tcgccaatca
     2401 gcttgggcta gagaaggatg tggttcgagt atggttctgt aaccggcgcc agaagggcaa
     2461 aagatcaagt attgagtatt cccaacgaga agagtatgag gctacaggga cacctttccc
     2521 agggggggct gtatcctttc ctctgccccc aggtccccac tttggcaccc caggctatgg
     2581 aagcccccac ttcaccacac tctactcagt cccttttcct gagggcgagg cctttccctc
     2641 tgttcccgtc actgctctgg gctctcccat gcattcaaac ggcggcgggt ccggaggaga
     2701 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcctggcc caatgtataa
     2761 catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg gcggcggcgg
     2821 aggaggcaac gccacggcgg cggcgaccgg cggcaaccag aagaacagcc cggaccgcgt
     2881 caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta agatggccca
     2941 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg agtggaaact
     3001 tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc gcgctctgca
     3061 catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga cgctcatgaa
     3121 gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca gcatggcgag
     3181 cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg acagctacgc
     3241 gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc tgggctaccc
     3301 gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc accgctacga
     3361 cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc
     3421 cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg gctccatggg
     3481 ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt cctcccactc
     3541 cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc tccccggcgc
     3601 cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact accagagcgg
     3661 cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg gcggcgggtc
     3721 cggaggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcctggccc
     3781 aatggctgtc agcgacgctc tgctcccgtc cttctccacg ttcgcgtccg gcccggcggg
     3841 aagggagaag acactgcgtc cagcaggtgc cccgactaac cgttggcgtg aggaactctc
     3901 tcacatgaag cgacttcccc cacttcccgg ccgcccctac gacctggcgg cgacggtggc
     3961 cacagacctg gagagtggcg gagctggtgc agcttgcagc agtaacaacc cggccctcct
     4021 agcccggagg gagaccgagg agttcaacga cctcctggac ctagacttta tcctttccaa
     4081 ctcgctaacc caccaggaat cggtggccgc caccgtgacc acctcggcgt cagcttcatc
     4141 ctcgtcttcc ccggcgagca gcggccctgc cagcgcgccc tccacctgca gcttcagcta
     4201 tccgatccgg gccgggggtg acccgggcgt ggctgccaga aacacaggtg gagggctcct
     4261 ctacagccga gaatctgcgc cacctcccac ggcccccttc aacctggggg acatcaatga
     4321 cgtgagcccc tcgggcggct tcgtggctga gctcctgcgg ccggagttgg acccagtata
     4381 cattccgcca cagcagcctc agccgccagg tggggggctg atgggcaagt ttgtgctgaa
     4441 ggcgtctctg accacccctg gcagcgagta cagcagccct tcggtcatca gtgttagcaa
     4501 aggaagccca gacggcagcc accccgtggt agtggcgccc tacagcggtg gcccgccgcg
     4561 catgtgcccc aagattaagc aagaggcggt cccgtcctgc acggtcagcc ggtccctaga
     4621 ggcccatttg agcgctggac cccagctcag caacggccac cggcccaaca cacacgactt
     4681 ccccctgggg cggcagctcc ccaccaggac tacccctaca ctgagtcccg aggaactgct
     4741 gaacagcagg gactgtcacc ctggcctgcc tcttccccca ggattccatc cccatccggg
     4801 ggccaactac cctcctttcc tgccagacca gatgcagtca caagtcccct ctctccatta
     4861 tcaagagctc atgccaccgg gttcctgcct gccagaggag cccaagccaa agaggggaag
     4921 aaggtcgtgg ccccggaaaa gaacagccac ccacacttgt gactatgcag gctgtggcaa
     4981 aacctatacc aagagttctc atctcaaggc acacctgcga actcacacag gcgagaaacc
     5041 ttaccactgt gactgggacg gctgtgggtg gaaattcgcc cgctccgatg aactgaccag
     5101 gcactaccgc aaacacacag ggcaccggcc ctttcagtgc cagaagtgtg acagggcctt
     5161 ttccaggtcg gaccaccttg ccttacacat gaagaggcac tttggcagcg gaaaacagct
     5221 gttgaatttt gaccttctca agttggcggg agacgtggag tccaacccag ggcccgtcga
     5281 gatgcccctc aacgtgaact tcaccaacag gaactatgac ctcgactacg actccgtaca
     5341 gccctatttc atctgcgacg aggaagagaa tttctatcac cagcaacagc agagcgagct
     5401 gcagccgccc gcgcccagtg aggatatctg gaagaaattc gagctgcttc ccaccccgcc
     5461 cctgtccccg agccgccgct ccgggctctg ctctccatcc tatgttgcgg tcgctacgtc
     5521 cttctcccca agggaagacg atgacggcgg cggtggcaac ttctccaccg ccgatcagct
     5581 ggagatgatg accgagttac ttggaggaga catggtgaac cagagcttca tctgcgatcc
     5641 tgacgacgag accttcatca agaacatcat catccaggac tgtatgtgga gcggtttctc
     5701 agccgctgcc aagctggtct cggagaagct ggcctcctac caggctgcgc gcaaagacag
     5761 caccagcctg agccccgccc gcgggcacag cgtctgctcc acctccagcc tgtacctgca
     5821 ggacctcacc gccgccgcgt ccgagtgcat tgacccctca gtggtctttc cctacccgct
     5881 caacgacagc agctcgccca aatcctgtac ctcgtccgat tccacggcct tctctccttc
     5941 ctcggactcg ctgctgtcct ccgagtcctc cccacgggcc agccctgagc ccctagtgct
     6001 gcatgaggag acaccgccca ccaccagcag cgactctgaa gaagagcaag aagatgagga
     6061 agaaattgat gtggtgtctg tggagaagag gcaaacccct gccaagaggt cggagtcggg
     6121 ctcatctcca tcccgaggcc acagcaaacc tccgcacagc ccactggtcc tcaagaggtg
     6181 ccacgtctcc actcaccagc acaactacgc cgcacccccc tccacaagga aggactatcc
     6241 agctgccaag agggccaagt tggacagtgg cagggtcctg aagcagatca gcaacaaccg
     6301 caagtgctcc agccccaggt cctcagacac ggaggaaaac gacaagaggc ggacacacaa
     6361 cgtcttggaa cgtcagagga ggaacgagct gaagcgcagc ttttttgccc tgcgtgacca
     6421 gatccctgaa ttggaaaaca acgaaaaggc ccccaaggta gtgatcctca aaaaagccac
     6481 cgcctacatc ctgtccattc aagcagacga gcacaagctc acctctgaaa aggacttatt
     6541 gaggaaacga cgagaacagt tgaaacacaa actcgaacag cttcgaaact ctggtgcagg
     6601 cggcgggtcc ggaggagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     6661 tcctggccca atgggctcgg tgtccaacca gcagtttgca ggtggctgcg ccaaggcagc
     6721 ggagaaggcg ccagaggagg cgccgcctga cgcggcccga gcggcagacg agccgcagct
     6781 gctgcacggg gccggcatct gtaagtggtt caacgtgcgc atggggttcg gcttcctgtc
     6841 tatgaccgcc cgcgccgggg tcgcgctcga ccccccggtg gacgtctttg tgcaccagag
     6901 caagctgcac atggaagggt tccgaagcct caaggagggt gaggcggtgg agttcacctt
     6961 taagaagtct gccaagggtc tggaatccat ccgtgtcact ggccctggtg gtgtgttctg
     7021 tattggaagt gagcggcggc caaaggggaa gaacatgcag aagcgaagat ccaaaggaga
     7081 caggtgctac aactgcggtg ggctagacca tcatgccaag gaatgcaagc tgccacccca
     7141 gcccaagaag tgccactttt gccaaagcat caaccatatg gtggcctcgt gtccactgaa
     7201 ggcccagcag ggccccagtt ctcagggaaa gcctgcctac ttccgggagg aagaggaaga
     7261 gatccacagc cctgccctgc tcccagaagc ccagaattga ctcgagatat ctagacccag
     7321 ctttcttgta caaagtggtg atctagctaa aacgcggcct cgaatcgaat tccgcccccc
     7381 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     7441 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7501 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7561 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7621 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7681 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7741 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7801 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7861 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     7921 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg
     7981 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     8041 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     8101 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     8161 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     8221 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     8281 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     8341 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     8401 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     8461 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     8521 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     8581 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     8641 ccactccacc ggcggcatgg acgagctgta caagtaaagc ggccgcgatc tttttccctc
     8701 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     8761 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     8821 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     8881 atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga
     8941 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     9001 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     9061 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     9121 tcttatgaag atccctcgac ctgcaggaat tcgatatcaa gctctagcca acaagctcgt
     9181 catcgctttg cagaagagca gagaggatat gctcatcgtc taaagaacta cccattttat
     9241 tatatattag tcacgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     9301 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     9361 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     9421 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     9481 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     9541 ttacgcagac tatctttcta gggttaaaaa agatttgcgc tttactcgac ctaaacttta
     9601 aacacgtcat agaatcttcg tttgacaaaa accacattgt ggccaagctg tgtgacgcga
     9661 cgcgcgctaa agaatggcaa accaagtcgc gcgaggtacc cagcttttgt tccctttagt
     9721 gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9781 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9841 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9901 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9961 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10021 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10081 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10141 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10201 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10261 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10321 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10381 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10441 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10501 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10561 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10621 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10681 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10741 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10801 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10861 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10921 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10981 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11041 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11101 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11161 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11221 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11281 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11341 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11401 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11461 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11521 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11581 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11641 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11701 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11761 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11821 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11881 catttccccg aaaagtgcca cctg
//
