LOCUS Exported 10330 bp ds-DNA circular SYN 07-MAR-2016 DEFINITION Donor vector targeting the human AAVS1 safe-harbor locus for constitutive eGFP and luciferase expression. P, puromycin selection cassette. HA-R (delT2), without remaining CRISPR protospacer and PAM. ACCESSION . VERSION . KEYWORDS KW625_pAAVS1-P-CAG-GFPluc2 SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 10330) AUTHORS Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K. TITLE Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. JOURNAL Methods 2015. PUBMED 26707206 REFERENCE 2 (bases 1 to 10330) AUTHORS Woltjen TITLE Direct Submission JOURNAL Exported Sunday, Jul 3, 2016 from SnapGene 3.1.4 http://www.snapgene.com COMMENT ID: RDB13738 Relation: eGFP only version: KW317 (RDB13737) Remarks: Used in combination with AAVS1 nucleases: CRISPR/Cas9 KW516 (RDB13729), or TALEN KW226 (RDB13730) and KW227 (RDB13731). FEATURES Location/Qualifiers source 1..10330 /organism="recombinant plasmid" /mol_type="other DNA" promoter 21..125 /gene="bla" /note="AmpR promoter" CDS 126..986 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1157..1745 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2033..2054 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 2069..2099 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 2107..2123 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2131..2147 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 2168..2186 /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" misc_feature 2199..3002 /note="HA-L" /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" misc_feature 3009..3034 /note="SA" /note="splice acceptor site" CDS 3058..3111 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3121..3720 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 3758..3982 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" enhancer 4001..4380 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 4382..4659 /note="chicken beta-actin promoter" intron 4660..5675 /note="chimeric intron" /note="chimera between introns from chicken beta-actin and rabbit beta-globin" protein_bind 5769..5793 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB1" /note="recombination site for the Gateway(R) BP reaction" CDS 5799..6515 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6540..8189 /codon_start=1 /product="firefly luciferase" /note="luciferase" /note="synthetic luc2 version of the luciferase gene" /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF VDEVPKGLTGKLDARKIREILIKAKKGGKIAV" protein_bind complement(8217..8241) /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB2" /note="recombination site for the Gateway(R) BP reaction" polyA_site 8402..8457 /note="beta-globin poly(A) signal" /note="rabbit beta-globin polyadenylation signal" misc_feature 8850..9669 /note="HA-R (delT2)" /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene; AAVS1 T2 sgRNA protospacer and PAM sequences removed (ACTAGGGACAGGATTGG)" promoter complement(9687..9705) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9712..9728) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin 9870..10325 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 2161 agctcgaaat taaccctcac taaagggaac aaaagctgtg ctttctctga ccagcattct 2221 ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct 2281 ggcccagatc cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc 2341 ttgctctctg ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc 2401 tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct 2461 ctcctccctc acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt 2521 ctggggcctg tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg 2581 cgtcccgcct ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag 2641 taccccgtct ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc 2701 ccgttctcct gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc 2761 cccttacctc tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg 2821 tccgagagct cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag 2881 catgtttgct gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg 2941 gccggttaat gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg 3001 gcaagcttct gacctcttct cttcctccca cagggcctcg agagatctgg cagcggagag 3061 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc taggctcgag 3121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 3181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 3241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 3301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 3361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 3421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 3481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 3541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 3601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 3661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 3721 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca 3781 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 3841 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 3901 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 3961 ggggatgcgg tgggctctat gggctagcgg tggcggcctc gacattgatt attgactagt 4021 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 4081 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 4141 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 4201 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 4261 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 4321 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 4381 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca 4441 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg 4501 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt 4561 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg 4621 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt 4681 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt 4741 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg 4801 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg 4861 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg 4921 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt 4981 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg 5041 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg 5101 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc 5161 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg 5221 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg 5281 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 5341 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 5401 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 5461 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 5521 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 5581 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 5641 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 5701 tgctgtctca tcattttggc aaagaattcg cggccgcggt accggcgcgc cggatcctgc 5761 aggttatcac aagtttgtac aaaaaagcag gctggaccat ggtgagcaag ggcgaggagc 5821 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 5881 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 5941 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 6001 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 6061 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 6121 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 6181 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 6241 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 6301 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 6361 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 6421 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 6481 ccgggatcac tctcggcatg gacgagctgt acaagggcgg tggaagatct gggaattcag 6541 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg 6601 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct 6661 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc 6721 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca 6781 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg 6841 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc 6901 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga 6961 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc 7021 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg 7081 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta 7141 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg 7201 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc 7261 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg 7321 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga 7381 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg 7441 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg 7501 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc 7561 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag 7621 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac 7681 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg 7741 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg 7801 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga 7861 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc 7921 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg 7981 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg 8041 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg 8101 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg 8161 ccaagaaggg cggcaagatc gccgtgtaat aattctagat tctgcagccc tatagcaccc 8221 agctttcttg tacaaagtgg tgataactct agagaattca ctcctcaggt gcaggctgcc 8281 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt 8341 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc 8401 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc 8461 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt 8521 ttggcaacat atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca 8581 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg 8641 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc 8701 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt 8761 ccctcttctc ttatgaagat ccctcgacct gcagcccaag cttggatccc tcgagttaat 8821 taaatctaga agtcgacagt actaagcttt gacagaaaag ccccatcctt aggcctcctc 8881 cttcctagtc tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct 8941 ggtgacacac ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc 9001 ttacgatgga gccagagagg atcctgggag ggagagcttg gcagggggtg ggagggaagg 9061 gggggatgcg tgacctgccc ggttctcagt ggccaccctg cgctaccctc tcccagaacc 9121 tgagctgctc tgacgcggct gtctggtgcg tttcactgat cctggtgctg cagcttcctt 9181 acacttccca agaggagaag cagtttggaa aaacaaaatc agaataagtt ggtcctgagt 9241 tctaactttg gctcttcacc tttctagtcc ccaatttata ttgttcctcc gtgcgtcagt 9301 tttacctgtg agataaggcc agtagccagc cccgtcctgg cagggctgtg gtgaggaggg 9361 gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt tcaccaggtc 9421 gtggccgcct ctactccctt tctctttctc catccttctt tccttaaaga gtccccagtg 9481 ctatctggga catattcctc cgcccagagc agggtcccgc ttccctaagg ccctgctctg 9541 ggcttctggg tttgagtcct tggcaagccc aggagaggcg ctcaggcttc cctgtccccc 9601 ttcctcgtcc accatctcat gcccctggct ctcctgcccc ttccctacag gggttcctgg 9661 ctctgctcta gcgatcgcca attcgcccta tagtgagtcg tattacaatt cactggccgt 9721 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 9781 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 9841 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 9901 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 9961 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 10021 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 10081 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 10141 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 10201 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 10261 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 10321 aatttaggtg //