LOCUS       Exported                7882 bp ds-DNA     circular SYN 13-JUL-2016
DEFINITION  pET151/D-Topo-ALIX_1-698.
ACCESSION   urn.local...1376250300641.10812
VERSION     .
KEYWORDS    WISP10-648_ALIXBro1V
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Path-IT
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 13, 2016 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    106..125
                     /label=pBRrevBam_primer
                     /note="pBRrevBam_primer"
     promoter        209..227
                     /label=T7_promoter
                     /note="T7_promoter"
     misc_feature    227..254
                     /label=lacO
                     /note="lacO"
     misc_feature    272..288
                     /label=T7_transl_en_RBS
                     /note="T7_transl_en_RBS"
     misc_feature    399..2517
                     /label=ALIX_1-698
                     /note="ALIX_1-698"
     terminator      2538..2666
                     /label=T7_terminator
                     /note="T7_terminator"
     misc_feature    complement(2577..2596)
                     /label=T7_Terminal_primer
                     /note="T7_Terminal_primer"
     promoter        3000..3028
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
     gene            3070..3930
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="Ampicillin"
     CDS             3070..3930
                     /codon_start=1
                     /label=ORF frame 3
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4085..4704
                     /label=pBR322_origin
                     /note="pBR322_origin"
     misc_feature    5103..5125
                     /label=pGEX_3_primer
                     /note="pGEX_3_primer"
     misc_feature    complement(5119..5310)
                     /label=ROP
                     /note="ROP"
     CDS             complement(5949..6566)
                     /codon_start=1
                     /label=ORF frame 3
                     /note="ORF frame 3"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIF
                     GEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA
                     FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPLI
                     VTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            complement(5949..6560)
                     /gene="tet (611 - 0)"
                     /label=tet (611 - 0)
                     /note="tet (611 - 0)"
     CDS             6144..6839
                     /codon_start=1
                     /label=ORF frame 2
                     /note="ORF frame 2"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQR
                     VGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCR
                     HLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVALTAR
                     FPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAASGPRW
                     FAPAGENPV"
     misc_feature    complement(6622..7713)
                     /label=lacI
                     /note="lacI"
     CDS             complement(6622..7581)
                     /codon_start=1
                     /label=ORF frame 2
                     /note="ORF frame 2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS
                     LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgc
      301 atcatcacca tcaccatggt aagcctatcc ctaaccctct cctcggtctc gattctacgg
      361 aaaacctgta ttttcaggga attgatccct tcacccatat ggcgacattc atctcggtgc
      421 agctgaaaaa gacctcagag gtggacctgg ccaagccgct ggtgaagttc atccagcaga
      481 cttacccaag cggcggggaa gagcaggccc agtactgccg cgcggcggag gagctcagca
      541 agctgcgccg cgccgcagtc ggtcgtccgc tggacaagca cgagggcgcg ctcgagacgc
      601 tcctgagata ttatgatcag atttgttcta ttgaacccaa attcccattt tctgaaaatc
      661 agatctgctt gacatttacc tggaaggatg ctttcgataa aggttcactt tttggaggct
      721 ctgtaaaact ggctcttgca agcttaggat atgaaaagag ctgtgtgttg ttcaattgtg
      781 cagccttagc tagccaaatt gcagcagaac agaacctgga taatgatgaa ggattgaaaa
      841 tcgctgctaa acattaccag tttgctagtg gtgccttttt acatattaaa gagacggttt
      901 tatctgcctt aagtcgagag ccgaccgtgg acatatctcc agatactgtt gggaccctca
      961 gtcttattat gctggcacag gctcaagaag tatttttttt aaaagccaca agagataaaa
     1021 tgaaagatgc catcatagct aaattggcta atcaggctgc agattatttt ggtgatgctt
     1081 tcaaacagtg tcaatacaaa gatactctcc ccaaggaggt gttccctgtc ttggctgcaa
     1141 agcactgtat catgcaggcc aatgctgagt accatcagtc tatcctggca aaacagcaga
     1201 agaaatttgg agaagaaatt gcaaggttac agcatgcagc agaactgatt aaaacagtgg
     1261 catctcgcta tgatgaatat gttaatgtga aggatttttc tgacaaaatc aatcgtgccc
     1321 ttgctgcagc aaagaaggat aatgacttca tttatcatga tcgagttcca gaccttaaag
     1381 atctagatcc tattggcaaa gccacacttg tgaaatctac cccggtcaat gtacccatca
     1441 gtcagaaatt tactgatctg tttgagaaga tggttcccgt gtcagtacag cagtctttgg
     1501 ctgcctataa tcagaggaaa gccgatttgg ttaacagatc aattgctcag atgagagaag
     1561 ccaccacttt ggcaaatggg gtgctagctt cccttaatct tccagcagca attgaagatg
     1621 tgtctggaga cactgtacct cagtctatat tgactaaatc cagatctgtg attgaacagg
     1681 gaggcatcca gactgttgat cagttgatta aagaactgcc tgaattactg caacgaaata
     1741 gagaaatcct agatgagtca ttaaggttgt tggatgaaga agaagcaacc gataatgatt
     1801 taagagcaaa atttaaggaa cgttggcaaa ggacaccatc caatgaactg tataagcctt
     1861 taagagcaga gggaaccaac ttcagaacag ttttagataa agctgtgcag gcagatggac
     1921 aagtgaaaga atgttaccag tctcatcgtg acaccatcgt gcttttgtgt aagccagagc
     1981 ctgagctgaa tgctgccatc ccttctgcta atccagcaaa gaccatgcag ggcagtgagg
     2041 ttgtaaatgt cttaaaatcc ttattgtcaa atcttgatga agtaaagaag gaaagagagg
     2101 gtctggagaa tgacttgaaa tctgtgaatt ttgacatgac aagcaagttt ttgacagccc
     2161 tggctcaaga tggtgtgata aatgaagaag ctctttctgt tactgaacta gatcgagtct
     2221 atggaggtct tacaactaaa gtccaagaat ctctaaagaa acaggaggga cttcttaaaa
     2281 atattcaggt ctcacatcag gaattttcaa aaatgaaaca atctaataat gaagctaact
     2341 taagagaaga agttttgaag aatttagcta ctgcgtatga caactttgtt gaacttgtag
     2401 ctaatttgaa ggaaggcaca aagttttaca atgagttgac tgaaatcctg gtcaggttcc
     2461 agaacaaatg cagtgatata gtttttgcac ggaagacaga aagataagga tccaattaag
     2521 ggcgagctca gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     2581 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     2641 gctgaaagga ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc
     2701 aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta
     2761 gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa
     2821 agctagctta tcgatgataa gctgtcaaac atgagaatta attcttgaag acgaaagggc
     2881 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2941 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3001 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3061 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3121 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3181 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3241 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3301 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3361 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3421 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3481 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3541 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3601 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3661 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3721 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3781 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3841 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3901 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3961 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4861 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4921 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     4981 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     5041 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5101 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     5161 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     5221 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     5281 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     5341 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     5401 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     5461 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     5521 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     5581 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     5641 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     5701 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     5761 gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct
     5821 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     5881 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg
     5941 gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag
     6001 acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg
     6061 gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg
     6121 agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     6181 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     6241 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata
     6301 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     6361 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     6421 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     6481 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     6541 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     6601 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     6721 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     6781 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     6841 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     6901 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     6961 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     7021 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     7081 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     7141 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     7201 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     7261 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     7321 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     7381 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     7441 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     7501 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     7561 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     7621 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     7681 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     7741 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     7801 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     7861 ccgccgcaag gaatggtgca tg
//
