LOCUS pDonor-tBFP-NLS-Neo 4761 bp DNA circular UNA 02-SEP-2016 DEFINITION Concatenation of 2 sequences (Circularized). ACCESSION urn:local:.:1451286303211.50 VERSION KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 606..>1314 /note="Geneious type: Blue FP" misc_feature 896 /note="Geneious type: Editing History Replacement" misc_feature 600..605 /note="Geneious type: Kozak" misc_feature 2638..3426 /note="Geneious type: Marker" /gene="NTP_II marker" misc_feature 606..1418 /note="Geneious type: ORF" misc_feature 4736..4738 /note="Geneious type: PAM" misc_feature 2261..2497 /note="Geneious type: Regulatory_Seq" /gene="SV40ER reg" misc_feature 4714..4739 /note="Geneious type: Site" Terminator 3604..3873 /gene="TK_PA term" misc_feature 1125..1127 /note="Geneious type: mutation" misc_feature 1315..1318 /note="Geneious type: ligation" misc_feature 1399..1402 /note="Geneious type: ligation" primer_bind 1198..1217 primer_bind 4566..4585 misc_feature complement(644..668) /note="Geneious type: primer_bind_reverse" misc_feature complement(1493..1513) /note="Geneious type: primer_bind_reverse" Promoter 7..588 /gene="CMV prom" Promoter 2284..2552 /gene="SV40 prom" Promoter 2172..2200 /gene="amp prom" Rep_Origin 4021..4640 /gene="pBR322 origin" Rep_Origin 1787..2093 /gene="f1 origin" Rep_Origin 2451..2528 /gene="SV40 origin" misc_feature 1326..1349 /note="Geneious type: Sorting signal" misc_feature 1350..1373 /note="Geneious type: Sorting signal" misc_feature 1374..1397 /note="Geneious type: Sorting signal" misc_feature 1403..1398 /note="Geneious type: Concatenated sequence" misc_feature <1399..1315 /note="Geneious type: Concatenated sequence" misc_feature 1319..>1398 /note="Geneious type: Concatenated sequence" misc_feature 2531..2543 /note="Geneious type: restriction site" misc_feature complement(4720..4725) /note="Geneious type: restriction site" misc_feature 4728..4733 /note="Geneious type: restriction site" misc_feature 1179..1184 /note="Geneious type: restriction site" misc_feature 2602..2607 /note="Geneious type: restriction site" misc_feature 1398..1403 /note="Geneious type: restriction site" misc_feature 232..237 /note="Geneious type: restriction site" misc_feature 1410..1415 /note="Geneious type: restriction site" misc_feature 1524..1529 /note="Geneious type: restriction site" misc_feature 1318..1323 /note="Geneious type: restriction site" misc_feature 1314..1319 /note="Geneious type: restriction site" misc_feature 1296..1301 /note="Geneious type: restriction site" ORIGIN 1 tagttataat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 541 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcggtcg 601 ccaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg 661 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc 721 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg 781 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact 841 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaggacg 901 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg 961 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg 1021 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg 1081 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata 1141 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac 1201 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg 1261 ccagatactg cgacctccct agcaaactgg ggcacaagct taattccgga ctcagatctc 1321 gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa 1381 aaaagaagag aaaggtagga tccaccggat ctagataact gatcataatc agccatacca 1441 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 1501 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 1561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 1621 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta 1681 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 1741 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 1801 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 1861 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 1921 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 1981 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 2041 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 2101 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 2161 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 2221 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt 2281 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 2341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2401 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 2461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 2521 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 2581 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt 2641 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 2701 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 2761 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac 2821 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 2881 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 2941 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 3001 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 3061 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 3121 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag 3181 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 3241 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 3301 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 3361 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 3421 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 3481 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 3541 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 3601 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga 3661 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg 3721 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg 3781 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg 3841 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga 3901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 3961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 4021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 4081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 4141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 4201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 4261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 4321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 4381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 4441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 4501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 4561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 4621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 4681 acatgttctt tcctgcgtta tcccctgatt ctgcaccggg tcttcgagaa gaccttggat 4741 aaccgtatta ccgccatgca t //