LOCUS       pDonor-tBFP-NLS-Neo        4761 bp    DNA     circular UNA 02-SEP-2016
DEFINITION  Concatenation of 2 sequences (Circularized).
ACCESSION   urn:local:.:1451286303211.50
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    606..>1314
                     /note="Geneious type: Blue FP"
     misc_feature    896
                     /note="Geneious type: Editing History Replacement"
     misc_feature    600..605
                     /note="Geneious type: Kozak"
     misc_feature    2638..3426
                     /note="Geneious type: Marker"
                     /gene="NTP_II marker"
     misc_feature    606..1418
                     /note="Geneious type: ORF"
     misc_feature    4736..4738
                     /note="Geneious type: PAM"
     misc_feature    2261..2497
                     /note="Geneious type: Regulatory_Seq"
                     /gene="SV40ER reg"
     misc_feature    4714..4739
                     /note="Geneious type: Site"
     Terminator      3604..3873
                     /gene="TK_PA term"
     misc_feature    1125..1127
                     /note="Geneious type: mutation"
     misc_feature    1315..1318
                     /note="Geneious type: ligation"
     misc_feature    1399..1402
                     /note="Geneious type: ligation"
     primer_bind     1198..1217
     primer_bind     4566..4585
     misc_feature    complement(644..668)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1493..1513)
                     /note="Geneious type: primer_bind_reverse"
     Promoter        7..588
                     /gene="CMV prom"
     Promoter        2284..2552
                     /gene="SV40 prom"
     Promoter        2172..2200
                     /gene="amp prom"
     Rep_Origin      4021..4640
                     /gene="pBR322 origin"
     Rep_Origin      1787..2093
                     /gene="f1 origin"
     Rep_Origin      2451..2528
                     /gene="SV40 origin"
     misc_feature    1326..1349
                     /note="Geneious type: Sorting signal"
     misc_feature    1350..1373
                     /note="Geneious type: Sorting signal"
     misc_feature    1374..1397
                     /note="Geneious type: Sorting signal"
     misc_feature    1403..1398
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <1399..1315
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1319..>1398
                     /note="Geneious type: Concatenated sequence"
     misc_feature    2531..2543
                     /note="Geneious type: restriction site"
     misc_feature    complement(4720..4725)
                     /note="Geneious type: restriction site"
     misc_feature    4728..4733
                     /note="Geneious type: restriction site"
     misc_feature    1179..1184
                     /note="Geneious type: restriction site"
     misc_feature    2602..2607
                     /note="Geneious type: restriction site"
     misc_feature    1398..1403
                     /note="Geneious type: restriction site"
     misc_feature    232..237
                     /note="Geneious type: restriction site"
     misc_feature    1410..1415
                     /note="Geneious type: restriction site"
     misc_feature    1524..1529
                     /note="Geneious type: restriction site"
     misc_feature    1318..1323
                     /note="Geneious type: restriction site"
     misc_feature    1314..1319
                     /note="Geneious type: restriction site"
     misc_feature    1296..1301
                     /note="Geneious type: restriction site"
ORIGIN      
        1 tagttataat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcggtcg
      601 ccaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
      661 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
      721 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
      781 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
      841 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaggacg
      901 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
      961 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     1021 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     1081 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     1141 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     1201 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     1261 ccagatactg cgacctccct agcaaactgg ggcacaagct taattccgga ctcagatctc
     1321 gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa
     1381 aaaagaagag aaaggtagga tccaccggat ctagataact gatcataatc agccatacca
     1441 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1501 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1621 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta
     1681 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     1741 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     1801 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     1861 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     1921 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     1981 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     2041 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     2101 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2161 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2221 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     2281 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2401 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2521 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2581 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     2641 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     2701 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     2761 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     2821 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     2881 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     2941 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     3001 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     3061 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     3121 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     3181 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     3241 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     3301 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     3361 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     3421 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     3481 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     3541 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     3601 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     3661 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     3721 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     3781 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     3841 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     3901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4681 acatgttctt tcctgcgtta tcccctgatt ctgcaccggg tcttcgagaa gaccttggat
     4741 aaccgtatta ccgccatgca t
//
