LOCUS pMA-gRNA1.0-8 2789 bp DNA circular UNA 28-AUG-2008 FEATURES Location/Qualifiers Site join(386..391,386^387) /site_type="restriction site" /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 368^369" /note="cut_0_on_negative_strand: 372^373" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI Site join(392..397,398^399) /site_type="restriction site" /note="Name: BsaI" /note="Pattern: ggtctc" /note="cut_0_on_positive_strand: 380^381" /note="cut_0_on_negative_strand: 384^385" /note="inhibited_by: 5': 5-methylcytosine" /note="site_type: other" /note="restriction site" /label=BsaI misc_feature 403..777 /feature_type="DNA/RNA Features" /label="G block" misc_feature 403..651 /feature_type="DNA/RNA Features" /label="U6 promoter" misc_feature 670..745 /feature_type="DNA/RNA Features" /label="chimeric gRNA scaffold" misc_feature 746..756 /feature_type="DNA/RNA Features" /label="U6 terminator" Site join(777^778,783..788) /site_type="restriction site" /note="Name: BsaI" /note="Pattern: ggtctc" /note="cut_0_on_positive_strand: 759^760" /note="cut_0_on_negative_strand: 763^764" /note="inhibited_by: 5': 5-methylcytosine" /note="site_type: other" /note="restriction site" /label=BsaI Site join(789..794,789^790) /site_type="restriction site" /note="Name: BsrGI" /note="Pattern: tgtaca" /note="cut_0_on_positive_strand: 771^772" /note="cut_0_on_negative_strand: 775^776" /note="inhibited_by: " /note="site_type: other" /note="restriction site" /label=BsrGI rep_origin complement(986..1653) /label="Col E1 origin" /vntifkey="33" CDS complement(1801..2661) /label="AmpR" /vntifkey="4" ORIGIN 1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGGCCGCT ACAGGGCGCT CCCATTCGCC ATTCAGGCTG CGCAACTGTT 181 GGGAAGGGCG TTTCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT 241 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 301 ACGGCCAGTG AGCGCGACGT AATACGACTC ACTATAGGGC GAATTGGCGG AAGGCCGTCA 361 AGGCCACGTG TCTTGTCCAG AGCTCGGATC CGGTCTCCGA AAGAGGGCCT ATTTCCCATG 421 ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG 481 ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG 541 TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA 601 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGGGTCTTCG 661 AGAAGACCTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT 721 TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TGTTTTAGAG CTAGAAATAG CAAGTTATCG 781 AAGAGACCTG TACAACGCGT GGTACCTGGA GCACAAGACT GGCCTCACGG GCCTTCCGCT 841 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAACAT GGTCATAGCT 901 GTTTCCTTGC GTATTGGGCG CTCTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 961 TTCGGGTAAA GCCTGGGGTG CCTAATGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 1021 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 1081 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 1141 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 1201 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 1261 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 1321 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 1381 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 1441 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 1501 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 1561 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 1621 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 1681 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 1741 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 1801 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 1861 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 1921 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 1981 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 2041 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 2101 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 2161 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 2221 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 2281 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 2341 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 2401 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 2461 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 2521 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 2581 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 2641 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 2701 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 2761 TCCGCGCACA TTTCCCCGAA AAGTGCCAC //