LOCUS       pLit*_cI5G6T,P_assemble 4924 bp ds-DNA     circular     28-OCT-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter            59..163
                         /label="AmpR promoter"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
     CDS                 164..1024
                         /label="AmpR"
                         /ApEinfo_revcolor=#faac61
                         /ApEinfo_fwdcolor=#faac61
     primer_bind         complement(382..401)
                         /label="Amp-R"
                         /ApEinfo_revcolor=#d6b295
                         /ApEinfo_fwdcolor=#d6b295
     CDS                 1107..2381
                         /label="M13 gene III"
                         /ApEinfo_revcolor=#85dae9
                         /ApEinfo_fwdcolor=#85dae9
     terminator          2396..2467
                         /label="rrnB T1 terminator"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
     terminator          2483..2510
                         /label="T7Te terminator"
                         /ApEinfo_revcolor=#f58a5e
                         /ApEinfo_fwdcolor=#f58a5e
     rep_origin          complement(2535..3044)
                         /label="M13 ori"
                         /ApEinfo_revcolor=#c7b0e3
                         /ApEinfo_fwdcolor=#c7b0e3
     primer_bind         complement(2730..2751)
                         /label="F1ori-F"
                         /ApEinfo_revcolor=#ffef86
                         /ApEinfo_fwdcolor=#ffef86
     primer_bind         2938..2957
                         /label="F1ori-R"
                         /ApEinfo_revcolor=#ff9ccd
                         /ApEinfo_fwdcolor=#ff9ccd
     rep_origin          3155..3743
                         /label="ori"
                         /ApEinfo_revcolor=#d6b295
                         /ApEinfo_fwdcolor=#d6b295
     primer_bind         3644..3663
                         /label="pBR322ori-F"
                         /ApEinfo_revcolor=#f8d3a9
                         /ApEinfo_fwdcolor=#f8d3a9
     source              3790..4924
                         /label="source"
                         /ApEinfo_revcolor=#d59687
                         /ApEinfo_fwdcolor=#d59687
     protein_bind        3804..3825
                         /label="CAP binding site"
                         /ApEinfo_revcolor=#c6c9d1
                         /ApEinfo_fwdcolor=#c6c9d1
     open reading frame  4052..4138
                         /label="C1 ORF "
                         /ApEinfo_revcolor=#f58a5e
                         /ApEinfo_fwdcolor=#f58a5e
     terminator          4780..4851
                         /label="rrnB T1 terminator"
                         /ApEinfo_revcolor=#c7b0e3
                         /ApEinfo_fwdcolor=#c7b0e3
     terminator          4867..4894
                         /label="T7Te terminator"
                         /ApEinfo_revcolor=#75c6a9
                         /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 cgcttcggcg ggcttttttt tgttaactac gtcaggtggc acttttcggg gaaatgtgcg
       61 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      121 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      181 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      241 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      301 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat
      361 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
      421 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      481 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      541 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      601 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      661 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      721 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      781 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      841 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tcccgcggta tcattgcagc
      901 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      961 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1021 gtaactgtca gaccaagttt actcatattt acggctagct cagtcctagg tatagtgcta
     1081 gcgaagacaa agatgagaaa tactaggtga aaaaattatt attcgcaatt cctttagttg
     1141 ttcctttcta ttctcactcc gctgaaactg ttgaaagttg tttagcaaaa tcccatacag
     1201 aaaattcatt tactaacgtc tggaaagacg acaaaacttt agatcgttac gctaactatg
     1261 agggctgtct gtggaatgct acaggcgttg tagtttgtac tggtgacgaa actcagtgtt
     1321 acggtacatg ggttcctatt gggcttgcta tccctgaaaa tgagggtggt ggctctgagg
     1381 gtggcggttc tgagggtggc ggttctgagg gtggcggtac taaacctcct gagtacggtg
     1441 atacacctat tccgggctat acttatatca accctctcga cggcacttat ccgcctggta
     1501 ctgagcaaaa ccccgctaat cctaatcctt ctcttgagga gtctcagcct cttaatactt
     1561 tcatgtttca gaataatagg ttccgaaata ggcagggggc attaactgtt tatacgggca
     1621 ctgttactca aggcactgac cccgttaaaa cttattacca gtacactcct gtatcatcaa
     1681 aagccatgta tgacgcttac tggaacggta aattcagaga ctgcgctttc cattctggct
     1741 ttaatgagga tttatttgtt tgtgaatatc aaggccaatc gtctgacctg cctcaacctc
     1801 ctgtcaatgc tggcggcggc tctggtggtg gttctggtgg cggctctgag ggtggtggct
     1861 ctgagggtgg cggttctgag ggtggcggct ctgagggagg cggttccggt ggtggctctg
     1921 gttccggtga ttttgattat gaaaagatgg caaacgctaa taagggggct atgaccgaaa
     1981 atgccgatga aaacgcgcta cagtctgacg ctaaaggcaa acttgattct gtcgctactg
     2041 attacggtgc tgctatcgat ggtttcattg gtgacgtttc cggccttgct aatggtaatg
     2101 gtgctactgg tgattttgct ggctctaatt cccaaatggc tcaagtcggt gacggtgata
     2161 attcaccttt aatgaataat ttccgtcaat atttaccttc cctccctcaa tcggttgaat
     2221 gtcgcccttt tgtctttggc gctggtaaac catatgaatt ttctattgat tgtgacaaaa
     2281 taaacttatt ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt atgtatgtat
     2341 tttctacgtt tgctaacata ctgcgtaata aggagtctta agaagaccca ggcatcaaat
     2401 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2461 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatata
     2521 ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg aagattgtat
     2581 aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
     2641 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2701 aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2761 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2821 aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2881 ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga gaaaggaagg
     2941 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     3001 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga
     3061 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3181 tctgctgctt acaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3301 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3781 tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattagaa ttcgctgaga
     3841 gacccgtgct gatcactgac cacaacgtgc gtgaaacact ggcggtttgt gaacgcgctt
     3901 atatcgtcag tcaggggcat ttgatcgccc acggcacgcc tacagaaatc ttacaagacg
     3961 aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt agaagtttgc
     4021 gacgttttag caggagagta cgattctgaa catgagcaca aaaaagaaac cattaacaca
     4081 agagcagctt gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact
     4141 tggcttatcc caggaattgg tcgcatacga gatggggatg tggcagtcca acttgtcggc
     4201 tttatttaat ggcatctcag cattaaatgc ttataacgcc gcattgcttg caaaaattct
     4261 caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga
     4321 agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt
     4381 tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg
     4441 ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc
     4501 catgaccgca ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga
     4561 ccctgagcag gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt
     4621 taccttcaag aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca
     4681 gtacccaatg atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca
     4741 gtggcctgaa gagacgtttg gctgagaaga cccaggcatc aaataaaacg aaaggctcag
     4801 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     4861 cacactggct caccttcggg tgggcctttc tgcgtttata ggtctcctgg cctgcagtaa
     4921 agcc
//