LOCUS       pZac-SMVP-Rma           6630 bp    DNA     circular     03-AUG-2016
Addgene 80931
Wei Leong Chew
George Church lab
FEATURES             Location/Qualifiers
     repeat_region   612..741
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
     misc_feature    908..1649
                     /note="CMV IE"
     protein_bind    914..927
                     /note="NF1"
     protein_bind    961..974
                     /note="NF1"
     protein_bind    1015..1028
                     /note="NF1"
     misc_feature    complement(801..884)
                     /note="SV40_enhancer"
     protein_bind    818..828
                     /note="NFkB2"
     promoter        1070..1622
                     /label=CMV_immearly_promoter
     misc_feature    1125..1412
                     /label=CAG_enhancer
     promoter        1568..1649
                     /label=CMV_promoter
     misc_feature    1579..1599
                     /label=CMV_fwd_primer
     TATA_signal     1614..1619
                     /note=""
     misc_feature    1720..1852
                     /label=chim_int
     gene            1888..4041
                     /note="hCas9"
     misc_feature    2044..2055
                     /note="Disordered"
     misc_feature    1891..2043
                     /note="C-intein"
     sig_peptide     4021..4041
                     /note="SV40 NLS"
     misc_feature    4009..4041
                     /note="NLS with linker"
     repeat_region   complement(4301..4430)
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
     misc_feature    complement(4052..4071)
                     /label=EBV_rev_primer
     3'UTR           4052..4253
                     /note="SV40 pA"
     polyA_signal    4151..4156
                     /note=""
     source          1..6630
                     /dnas_title="pZac-SMVP-Rma Int-C - SphCas9 -NLS SV40pA
                     (Addgene 80931)"
ORIGIN      
        1 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
       61 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      121 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      181 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctgcgg ttttgctcac
      241 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      301 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      361 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      421 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      481 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      541 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaga
      601 tttaattaag gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      661 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      721 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacCT
      781 GCGGCCGCCA TTATGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
      841 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAgtcccg gtctcctccc
      901 atgcatgtca atattggcca ttagccatat tattcattgg ttatatagca taaatcaata
      961 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     1021 catgtccaat atgaccgcca tgttggcatt gattattgac tagttattaa tagtaatcaa
     1081 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1141 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1201 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1261 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     1321 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     1381 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1441 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1501 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1561 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1621 gcagaggtcg tttagtgaac cgtcagatca ctagtagctt tattgcggta gtttatcaca
     1681 gttaaattgc taacgcagtc agtgctcgac tgatcacagg taagtatcaa ggttacaaga
     1741 caggtttaag gaggccaata gaaactgggc ttgtcgagac agagaagatt cttgcgtttc
     1801 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggGGTACCG
     1861 AAGCCGCTAG CGCTACCGGT CGCCACCatg gcggcggcgt gcccggaact gcgtcagctg
     1921 gcgcagagcg atgtgtattg ggatccgatt gtgagcattg aaccggatgg cgtggaagaa
     1981 gtgtttgatc tgaccgtgcc gggcccgcat aactttgtgg cgaacgatat tattgcgcat
     2041 aacTCTGGCC AGGGGGACAG TCTTCACGAG CACATCGCTA ATCTTGCAGG TAGCCCAGCT
     2101 ATCAAAAAGG GAATACTGCA GACCGTTAAG GTCGTGGATG AACTCGTCAA AGTAATGGGA
     2161 AGGCATAAGC CCGAGAATAT CGTTATCGAG ATGGCCCGAG AGAACCAAAC TACCCAGAAG
     2221 GGACAGAAGA ACAGTAGGGA AAGGATGAAG AGGATTGAAG AGGGTATAAA AGAACTGGGG
     2281 TCCCAAATCC TTAAGGAACA CCCAGTTGAA AACACCCAGC TTCAGAATGA GAAGCTCTAC
     2341 CTGTACTACC TGCAGAACGG CAGGGACATG TACGTGGATC AGGAACTGGA CATCAATCGG
     2401 CTCTCCGACT ACGACGTGGA TCATATCGTG CCCCAGTCTT TTCTCAAAGA TGATTCTATT
     2461 GATAATAAAG TGTTGACAAG ATCCGATAAA AATAGAGGGA AGAGTGATAA CGTCCCCTCA
     2521 GAAGAAGTTG TCAAGAAAAT GAAAAATTAT TGGCGGCAGC TGCTGAACGC CAAACTGATC
     2581 ACACAACGGA AGTTCGATAA TCTGACTAAG GCTGAACGAG GTGGCCTGTC TGAGTTGGAT
     2641 AAAGCCGGCT TCATCAAAAG GCAGCTTGTT GAGACACGCC AGATCACCAA GCACGTGGCC
     2701 CAAATTCTCG ATTCACGCAT GAACACCAAG TACGATGAAA ATGACAAACT GATTCGAGAG
     2761 GTGAAAGTTA TTACTCTGAA GTCTAAGCTG GTcTCAGATT TCAGAAAGGA CTTTCAGTTT
     2821 TATAAGGTGA GAGAGATCAA CAATTACCAC CATGCGCATG ATGCCTACCT GAATGCAGTG
     2881 GTAGGCACTG CACTTATCAA AAAATATCCC AAGCTTGAAT CTGAATTTGT TTACGGAGAC
     2941 TATAAAGTGT ACGATGTTAG GAAAATGATC GCAAAGTCTG AGCAGGAAAT AGGCAAGGCC
     3001 ACCGCTAAGT ACTTCTTTTA CAGCAATATT ATGAATTTTT TCAAGACCGA GATTACACTG
     3061 GCCAATGGAG AGATTCGGAA GCGACCACTT ATCGAAACAA ACGGAGAAAC AGGAGAAATC
     3121 GTGTGGGACA AGGGTAGGGA TTTCGCGACA GTCCGGAAGG TCCTGTCCAT GCCGCAGGTG
     3181 AACATCGTTA AAAAGACCGA AGTACAGACC GGAGGCTTCT CCAAGGAAAG TATCCTCCCG
     3241 AAAAGGAACA GCGACAAGCT GATCGCACGC AAAAAAGATT GGGACCCCAA GAAATACGGC
     3301 GGATTCGATT CTCCTACAGT CGCTTACAGT GTACTGGTTG TGGCCAAAGT GGAGAAAGGG
     3361 AAGTCTAAAA AACTCAAAAG CGTCAAGGAA CTGCTGGGCA TCACAATCAT GGAGCGATCA
     3421 AGCTTCGAAA AAAACCCCAT CGACTTTCTC GAGGCGAAAG GATATAAAGA GGTCAAAAAA
     3481 GACCTCATCA TTAAGCTTCC CAAGTACTCT CTCTTTGAGC TTGAAAACGG CCGGAAACGA
     3541 ATGCTCGCTA GTGCGGGCGA GCTGCAGAAA GGTAACGAGC TGGCACTGCC CTCTAAATAC
     3601 GTTAATTTCT TGTATCTGGC CAGCCACTAT GAAAAGCTCA AAGGGTCtCC CGAAGATAAT
     3661 GAGCAGAAGC AGCTGTTCGT GGAACAACAC AAACACTACC TTGATGAGAT CATCGAGCAA
     3721 ATAAGCGAAT TCTCCAAAAG AGTGATCCTC GCCGACGCTA ACCTCGATAA GGTGCTTTCT
     3781 GCTTACAATA AGCACAGGGA TAAGCCCATC AGGGAGCAGG CAGAAAACAT TATCCACTTG
     3841 TTTACTCTGA CCAACTTGGG CGCGCCTGCA GCCTTCAAGT ACTTCGACAC CACCATAGAC
     3901 AGAAAGCGGT ACACCTCTAC AAAGGAGGTC CTGGACGCCA CACTGATTCA TCAGTCAATT
     3961 ACGGGGCTCT ATGAAACAAG AATCGACCTC TCTCAGCTCG GTGGAGACAG CAGGGCTGAC
     4021 CCCAAGAAGA AGAGGAAGGT GTCTCGAGCT cgatgagttt ggacaaacca caactagaat
     4081 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4141 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4201 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtagcGGCCG
     4261 CAGgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
     4321 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4381 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa
     4441 cctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4501 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4561 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     4621 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4681 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4741 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4801 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4861 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4921 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4981 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5041 aaaatattaa cgtttataat ttcaggtggc atctttcggg gaaatgtgcg cggaacccct
     5101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5161 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5221 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5281 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5341 aatagtggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5401 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5461 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5521 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5581 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5641 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5701 gccataccaa acgacgagcg tgacaccacg atgcctgtag taatggtaac aacgttgcgc
     5761 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5821 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5881 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5941 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6001 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6061 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6121 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6181 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6241 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6301 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6361 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6421 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6481 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6541 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6601 tacctacagc gtgagctatg agaaagcgcc
//

