LOCUS       pSMVP                   5409 bp    DNA     circular     03-AUG-2016
Addgene 80934
Wei Leong Chew
George Church lab
FEATURES             Location/Qualifiers
     promoter        314..866
                     /label=CMV_immearly_promoter
     misc_feature    369..656
                     /label=CAG_enhancer
     misc_feature    152..893
                     /note="CMV IE"
     protein_bind    158..171
                     /note="NF1"
     protein_bind    205..218
                     /note="NF1"
     protein_bind    259..272
                     /note="NF1"
     misc_feature    1..33
                     /note="KanR promoter"
     promoter        812..893
                     /label=CMV_promoter
     misc_feature    823..843
                     /label=CMV_fwd_primer
     misc_feature    964..1096
                     /label=chim_int
     TATA_signal     858..863
                     /note=""
     misc_feature    complement(3638..3657)
                     /label=EBV_rev_primer
     rep_origin      complement(3931..4550)
                     /label=pBR322_origin
     3'UTR           3638..3839
                     /note="SV40 pA"
     polyA_signal    3737..3742
                     /note=""
     gene            complement(4618..5397)
                     /dnas_title="NeoR/KanR (variant)"
                     /label="NeoR/KanR (variant)"
                     /gene="NeoR/KanR (variant)"
     misc_feature    complement(39..59)
                     /label=pBABE_3_primer
     misc_feature    complement(45..128)
                     /label=SV40_enhancer
     protein_bind    62..72
                     /note="NFkB2"
     gene            1132..3318
                     /note="hCas9"
     misc_feature    1138..1161
                     /label=SV40 NLS
     misc_feature    3316..3318
                     /note=""
     misc_feature    3319..3624
                     /note="N-intein"
     source          1..5409
                     /dnas_title="pSMVP - NLS SphCas9 - Rma Int-N - SV40pA
                     (Addgene 80936)"
ORIGIN      
        1 aatattattg aagcatttat cagggttcgt ctcgCTGTGG AATGTGTGTC AGTTAGGGTG
       61 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC
      121 AGCAACCAgt cccggtctcc tcccatgcat gtcaatattg gccattagcc atattattca
      181 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
      241 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
      301 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      541 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      601 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      661 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
      721 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      781 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
      841 gtgtacggtg ggaggtctat ataagcagag gtcgtttagt gaaccgtcag atcactagta
      901 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct cgactgatca
      961 caggtaagta tcaaggttac aagacaggtt taaggaggcc aatagaaact gggcttgtcg
     1021 agacagagaa gattcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     1081 gcctttctct ccacaggGGT ACCGAAGCCG CTAGCGCTAC CGGTCGCCAC CATGGCCCCA
     1141 AAGAAGAAGC GGAAGGTCGG TATCCACGGA GTCCCAGCAG CCGACAAGAA GTACTCCATT
     1201 GGGCTCGATA TCGGCACAAA CAGCGTCGGC TGGGCCGTCA TTACGGACGA GTACAAGGTG
     1261 CCGAGCAAAA AATTCAAAGT TCTGGGCAAT ACCGATCGCC ACAGCATAAA GAAGAACCTC
     1321 ATTGGCGCCC TCCTGTTCGA CTCCGGGGAa ACGGCCGAAG CCACGCGGCT CAAAAGAACA
     1381 GCACGGCGCA GATATACCCG CAGAAAGAAT CGGATCTGCT ACCTGCAGGA GATCTTTAGT
     1441 AATGAGATGG CTAAGGTGGA TGACTCTTTC TTCCATAGGC TGGAGGAGTC CTTTTTGGTG
     1501 GAGGAGGATA AAAAGCACGA GCGCCACCCA ATCTTTGGCA ATATCGTGGA CGAGGTGGCG
     1561 TACCATGAAA AGTACCCAAC CATATATCAT CTGAGGAAGA AGCTTGTAGA CAGTACTGAT
     1621 AAGGCTGACT TGCGGTTGAT CTATCTCGCG CTGGCGCATA TGATCAAATT TCGGGGACAC
     1681 TTCCTCATCG AGGGGGACCT GAACCCAGAC AACAGCGATG TCGACAAACT CTTTATCCAA
     1741 CTGGTTCAGA CTTACAATCA GCTTTTCGAA GAGAACCCGA TCAACGCATC CGGAGTTGAC
     1801 GCCAAAGCAA TCCTGAGCGC TAGGCTGTCC AAATCCCGGC GGCTCGAAAA CCTCATCGCA
     1861 CAGCTCCCTG GGGAGAAGAA GAACGGCCTG TTTGGTAATC TTATCGCCCT GTCACTCGGG
     1921 CTGACCCCCA ACTTTAAATC TAACTTCGAC CTGGCCGAAG ATGCCAAGCT TCAACTGAGC
     1981 AAAGACACCT ACGATGATGA TCTCGACAAT CTGCTGGCCC AGATCGGCGA CCAGTACGCA
     2041 GACCTTTTTT TGGCGGCAAA GAACCTGTCA GACGCCATTC TGCTGAGTGA TATTCTGCGA
     2101 GTGAACACGG AGATCACCAA AGCTCCGCTG AGCGCTAGTA TGATCAAGCG CTATGATGAG
     2161 CACCACCAAG ACTTGACTTT GCTGAAGGCC CTTGTCAGAC AGCAACTGCC TGAGAAGTAC
     2221 AAGGAAATTT TCTTCGATCA GTCTAAAAAT GGCTACGCCG GATACATTGA CGGCGGAGCA
     2281 AGCCAGGAGG AATTTTACAA ATTTATTAAG CCCATCTTGG AAAAAATGGA CGGCACCGAG
     2341 GAGCTGCTGG TAAAGCTTAA CAGAGAAGAT CTGTTGCGCA AACAGCGCAC TTTCGACAAT
     2401 GGAAGCATCC CCCACCAGAT TCACCTGGGC GAACTGCACG CTATCCTCAG GCGGCAAGAG
     2461 GATTTCTACC CCTTTTTGAA AGATAACAGG GAAAAGATTG AGAAAATCCT CACATTTCGG
     2521 ATACCCTACT ATGTAGGCCC CCTCGCCCGG GGAAATTCCA GATTCGCGTG GATGACTCGC
     2581 AAATCAGAAG AgACCATCAC TCCCTGGAAC TTCGAGGAAG TCGTGGATAA GGGGGCCTCT
     2641 GCCCAGTCCT TCATCGAAAG GATGACTAAC TTTGATAAAA ATCTGCCTAA CGAAAAGGTG
     2701 CTTCCTAAAC ACTCTCTGCT GTACGAGTAC TTCACAGTTT ATAACGAGCT CACCAAGGTC
     2761 AAATACGTCA CAGAAGGGAT GAGAAAGCCA GCATTCCTGT CTGGAGAGCA GAAGAAAGCT
     2821 ATCGTGGACC TCCTCTTCAA GACGAACCGG AAAGTTACCG TGAAACAGCT CAAAGAAGAC
     2881 TATTTCAAAA AGATTGAATG TTTCGACTCT GTTGAAATCA GCGGAGTGGA GGATCGCTTC
     2941 AACGCATCCC TGGGAACGTA TCACGATCTC CTGAAAATCA TTAAAGACAA GGACTTCCTG
     3001 GACAATGAGG AGAACGAGGA CATTCTTGAG GACATTGTCC TCACCCTTAC GTTGTTTGAA
     3061 GATAGGGAGA TGATTGAAGA ACGCTTGAAA ACTTACGCTC ATCTCTTCGA CGACAAAGTC
     3121 ATGAAACAGC TCAAGAGGCG CCGATATACA GGATGGGGGC GGCTGTCAAG AAAACTGATC
     3181 AATGGGATCC GAGACAAGCA GAGTGGAAAG ACAATCCTGG ATTTTCTTAA GTCCGATGGA
     3241 TTTGCCAACC GGAACTTCAT GCAGTTGATC CATGATGACT CTCTCACCTT TAAGGAGGAC
     3301 ATCCAGAAAG CACAAGTTtg tctggctggc gatactctca ttaccctggc cgatggacga
     3361 cgagtgccta ttagagaact ggtgtcacag cagaattttt ccgtgtgggc tctgaatcct
     3421 cagacttacc gcctggagag ggctagagtg agtagagctt tctgtaccgg catcaaacct
     3481 gtgtaccgcc tcaccactag actggggaga tccattaggg ccactgccaa ccaccgattt
     3541 ctcacacctc agggctggaa acgagtcgat gaactccagc ctggagatta cctggctctg
     3601 cctaggagaa tccctactgc ctcctgaTCT CGAGCTcgat gagtttggac aaaccacaac
     3661 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3721 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3781 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtac
     3841 ttaagagggg gagaccaaag ggcgagacgt taaggcctca cgtgacatgt gagcaaaagg
     3901 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3961 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4021 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4081 cctgccgctt acGggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4141 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4201 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4261 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4321 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4381 tagaagAaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4441 tggtagctct tgatccggca aacaaaccaC cgctggtagc ggtggttttt ttgtttgcaa
     4501 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4561 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc cgtctcagaa
     4621 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     4681 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     4741 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     4801 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     4861 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc
     4921 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     4981 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     5041 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     5101 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     5161 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     5221 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     5281 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     5341 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     5401 ttcaatcat
//

