LOCUS       pSMVP                   7869 bp    DNA     circular     03-AUG-2016
Addgene 80941
Wei Leong Chew
George Church lab
FEATURES             Location/Qualifiers
     promoter        350..902
                     /label=CMV_immearly_promoter
     misc_feature    405..692
                     /label=CAG_enhancer
     misc_feature    188..929
                     /note="CMV IE"
     protein_bind    194..207
                     /note="NF1"
     protein_bind    241..254
                     /note="NF1"
     protein_bind    295..308
                     /note="NF1"
     misc_feature    1..33
                     /note="KanR promoter"
     promoter        848..929
                     /label=CMV_promoter
     misc_feature    859..879
                     /label=CMV_fwd_primer
     misc_feature    1000..1132
                     /label=chim_int
     TATA_signal     894..899
                     /note=""
     gene            1168..5304
                     /note="hCas9"
     misc_feature    5353..5418
                     /note="P2A"
     sig_peptide     5284..5304
                     /note="SV40 NLS"
     misc_feature    5272..5304
                     /note="NLS with linker"
     sig_peptide     5305..5328
                     /note="SV40 NLS"
     sig_peptide     5329..5352
                     /note="SV40 NLS"
     misc_feature    complement(6098..6117)
                     /label=EBV_rev_primer
     3'UTR           6098..6299
                     /note="SV40 pA"
     rep_origin      complement(6391..7010)
                     /label=pBR322_origin
     polyA_signal    6197..6202
                     /note=""
     gene            complement(7078..7857)
                     /note="NeoR/KanR (variant)"
     misc_feature    complement(81..164)
                     /label=SV40_enhancer
     protein_bind    98..108
                     /note="NFkB2"
     source          1..7869
                     /dnas_title="pSMVP SphCas9 3xNLS P2A turboGFP (Addgene
                     80941)"
ORIGIN      
        1 aatattattg aagcatttat cagggttcgt ctcgtcccTC TAGACCCAGC TTTCTTGTAC
       61 AAAGTTGGCA TTATGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
      121 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAgtcccg gtctcctccc
      181 atgcatgtca atattggcca ttagccatat tattcattgg ttatatagca taaatcaata
      241 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
      301 catgtccaat atgaccgcca tgttggcatt gattattgac tagttattaa tagtaatcaa
      361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
      601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
      661 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      721 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      781 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      841 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      901 gcagaggtcg tttagtgaac cgtcagatca ctagtagctt tattgcggta gtttatcaca
      961 gttaaattgc taacgcagtc agtgctcgac tgatcacagg taagtatcaa ggttacaaga
     1021 caggtttaag gaggccaata gaaactgggc ttgtcgagac agagaagatt cttgcgtttc
     1081 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggGGTACCG
     1141 AAGCCGCTAG CGCTACCGGT CGCCACCATG GACAAGAAGT ACTCCATTGG GCTCGATATC
     1201 GGCACAAACA GCGTCGGCTG GGCCGTCATT ACGGACGAGT ACAAGGTGCC GAGCAAAAAA
     1261 TTCAAAGTTC TGGGCAATAC CGATCGCCAC AGCATAAAGA AGAACCTCAT TGGCGCCCTC
     1321 CTGTTCGACT CCGGGGAaAC GGCCGAAGCC ACGCGGCTCA AAAGAACAGC ACGGCGCAGA
     1381 TATACCCGCA GAAAGAATCG GATCTGCTAC CTGCAGGAGA TCTTTAGTAA TGAGATGGCT
     1441 AAGGTGGATG ACTCTTTCTT CCATAGGCTG GAGGAGTCCT TTTTGGTGGA GGAGGATAAA
     1501 AAGCACGAGC GCCACCCAAT CTTTGGCAAT ATCGTGGACG AGGTGGCGTA CCATGAAAAG
     1561 TACCCAACCA TATATCATCT GAGGAAGAAG CTTGTAGACA GTACTGATAA GGCTGACTTG
     1621 CGGTTGATCT ATCTCGCGCT GGCGCATATG ATCAAATTTC GGGGACACTT CCTCATCGAG
     1681 GGGGACCTGA ACCCAGACAA CAGCGATGTC GACAAACTCT TTATCCAACT GGTTCAGACT
     1741 TACAATCAGC TTTTCGAAGA GAACCCGATC AACGCATCCG GAGTTGACGC CAAAGCAATC
     1801 CTGAGCGCTA GGCTGTCCAA ATCCCGGCGG CTCGAAAACC TCATCGCACA GCTCCCTGGG
     1861 GAGAAGAAGA ACGGCCTGTT TGGTAATCTT ATCGCCCTGT CACTCGGGCT GACCCCCAAC
     1921 TTTAAATCTA ACTTCGACCT GGCCGAAGAT GCCAAGCTTC AACTGAGCAA AGACACCTAC
     1981 GATGATGATC TCGACAATCT GCTGGCCCAG ATCGGCGACC AGTACGCAGA CCTTTTTTTG
     2041 GCGGCAAAGA ACCTGTCAGA CGCCATTCTG CTGAGTGATA TTCTGCGAGT GAACACGGAG
     2101 ATCACCAAAG CTCCGCTGAG CGCTAGTATG ATCAAGCGCT ATGATGAGCA CCACCAAGAC
     2161 TTGACTTTGC TGAAGGCCCT TGTCAGACAG CAACTGCCTG AGAAGTACAA GGAAATTTTC
     2221 TTCGATCAGT CTAAAAATGG CTACGCCGGA TACATTGACG GCGGAGCAAG CCAGGAGGAA
     2281 TTTTACAAAT TTATTAAGCC CATCTTGGAA AAAATGGACG GCACCGAGGA GCTGCTGGTA
     2341 AAGCTTAACA GAGAAGATCT GTTGCGCAAA CAGCGCACTT TCGACAATGG AAGCATCCCC
     2401 CACCAGATTC ACCTGGGCGA ACTGCACGCT ATCCTCAGGC GGCAAGAGGA TTTCTACCCC
     2461 TTTTTGAAAG ATAACAGGGA AAAGATTGAG AAAATCCTCA CATTTCGGAT ACCCTACTAT
     2521 GTAGGCCCCC TCGCCCGGGG AAATTCCAGA TTCGCGTGGA TGACTCGCAA ATCAGAAGAg
     2581 ACCATCACTC CCTGGAACTT CGAGGAAGTC GTGGATAAGG GGGCCTCTGC CCAGTCCTTC
     2641 ATCGAAAGGA TGACTAACTT TGATAAAAAT CTGCCTAACG AAAAGGTGCT TCCTAAACAC
     2701 TCTCTGCTGT ACGAGTACTT CACAGTTTAT AACGAGCTCA CCAAGGTCAA ATACGTCACA
     2761 GAAGGGATGA GAAAGCCAGC ATTCCTGTCT GGAGAGCAGA AGAAAGCTAT CGTGGACCTC
     2821 CTCTTCAAGA CGAACCGGAA AGTTACCGTG AAACAGCTCA AAGAAGACTA TTTCAAAAAG
     2881 ATTGAATGTT TCGACTCTGT TGAAATCAGC GGAGTGGAGG ATCGCTTCAA CGCATCCCTG
     2941 GGAACGTATC ACGATCTCCT GAAAATCATT AAAGACAAGG ACTTCCTGGA CAATGAGGAG
     3001 AACGAGGACA TTCTTGAGGA CATTGTCCTC ACCCTTACGT TGTTTGAAGA TAGGGAGATG
     3061 ATTGAAGAAC GCTTGAAAAC TTACGCTCAT CTCTTCGACG ACAAAGTCAT GAAACAGCTC
     3121 AAGAGGCGCC GATATACAGG ATGGGGGCGG CTGTCAAGAA AACTGATCAA TGGGATCCGA
     3181 GACAAGCAGA GTGGAAAGAC AATCCTGGAT TTTCTTAAGT CCGATGGATT TGCCAACCGG
     3241 AACTTCATGC AGTTGATCCA TGATGACTCT CTCACCTTTA AGGAGGACAT CCAGAAAGCA
     3301 CAAGTTTCTG GCCAGGGGGA CAGTCTTCAC GAGCACATCG CTAATCTTGC AGGTAGCCCA
     3361 GCTATCAAAA AGGGAATACT GCAGACCGTT AAGGTCGTGG ATGAACTCGT CAAAGTAATG
     3421 GGAAGGCATA AGCCCGAGAA TATCGTTATC GAGATGGCCC GAGAGAACCA AACTACCCAG
     3481 AAGGGACAGA AGAACAGTAG GGAAAGGATG AAGAGGATTG AAGAGGGTAT AAAAGAACTG
     3541 GGGTCCCAAA TCCTTAAGGA ACACCCAGTT GAAAACACCC AGCTTCAGAA TGAGAAGCTC
     3601 TACCTGTACT ACCTGCAGAA CGGCAGGGAC ATGTACGTGG ATCAGGAACT GGACATCAAT
     3661 CGGCTCTCCG ACTACGACGT GGATCATATC GTGCCCCAGT CTTTTCTCAA AGATGATTCT
     3721 ATTGATAATA AAGTGTTGAC AAGATCCGAT AAAAATAGAG GGAAGAGTGA TAACGTCCCC
     3781 TCAGAAGAAG TTGTCAAGAA AATGAAAAAT TATTGGCGGC AGCTGCTGAA CGCCAAACTG
     3841 ATCACACAAC GGAAGTTCGA TAATCTGACT AAGGCTGAAC GAGGTGGCCT GTCTGAGTTG
     3901 GATAAAGCCG GCTTCATCAA AAGGCAGCTT GTTGAGACAC GCCAGATCAC CAAGCACGTG
     3961 GCCCAAATTC TCGATTCACG CATGAACACC AAGTACGATG AAAATGACAA ACTGATTCGA
     4021 GAGGTGAAAG TTATTACTCT GAAGTCTAAG CTGGTcTCAG ATTTCAGAAA GGACTTTCAG
     4081 TTTTATAAGG TGAGAGAGAT CAACAATTAC CACCATGCGC ATGATGCCTA CCTGAATGCA
     4141 GTGGTAGGCA CTGCACTTAT CAAAAAATAT CCCAAGCTTG AATCTGAATT TGTTTACGGA
     4201 GACTATAAAG TGTACGATGT TAGGAAAATG ATCGCAAAGT CTGAGCAGGA AATAGGCAAG
     4261 GCCACCGCTA AGTACTTCTT TTACAGCAAT ATTATGAATT TTTTCAAGAC CGAGATTACA
     4321 CTGGCCAATG GAGAGATTCG GAAGCGACCA CTTATCGAAA CAAACGGAGA AACAGGAGAA
     4381 ATCGTGTGGG ACAAGGGTAG GGATTTCGCG ACAGTCCGGA AGGTCCTGTC CATGCCGCAG
     4441 GTGAACATCG TTAAAAAGAC CGAAGTACAG ACCGGAGGCT TCTCCAAGGA AAGTATCCTC
     4501 CCGAAAAGGA ACAGCGACAA GCTGATCGCA CGCAAAAAAG ATTGGGACCC CAAGAAATAC
     4561 GGCGGATTCG ATTCTCCTAC AGTCGCTTAC AGTGTACTGG TTGTGGCCAA AGTGGAGAAA
     4621 GGGAAGTCTA AAAAACTCAA AAGCGTCAAG GAACTGCTGG GCATCACAAT CATGGAGCGA
     4681 TCAAGCTTCG AAAAAAACCC CATCGACTTT CTCGAGGCGA AAGGATATAA AGAGGTCAAA
     4741 AAAGACCTCA TCATTAAGCT TCCCAAGTAC TCTCTCTTTG AGCTTGAAAA CGGCCGGAAA
     4801 CGAATGCTCG CTAGTGCGGG CGAGCTGCAG AAAGGTAACG AGCTGGCACT GCCCTCTAAA
     4861 TACGTTAATT TCTTGTATCT GGCCAGCCAC TATGAAAAGC TCAAAGGGTC tCCCGAAGAT
     4921 AATGAGCAGA AGCAGCTGTT CGTGGAACAA CACAAACACT ACCTTGATGA GATCATCGAG
     4981 CAAATAAGCG AATTCTCCAA AAGAGTGATC CTCGCCGACG CTAACCTCGA TAAGGTGCTT
     5041 TCTGCTTACA ATAAGCACAG GGATAAGCCC ATCAGGGAGC AGGCAGAAAA CATTATCCAC
     5101 TTGTTTACTC TGACCAACTT GGGCGCGCCT GCAGCCTTCA AGTACTTCGA CACCACCATA
     5161 GACAGAAAGC GGTACACCTC TACAAAGGAG GTCCTGGACG CCACACTGAT TCATCAGTCA
     5221 ATTACGGGGC TCTATGAAAC AAGAATCGAC CTCTCTCAGC TCGGTGGAGA CAGCAGGGCT
     5281 GACCCCAAGA AGAAGAGGAA GGTGGATCCA AAAAAGAAGA GgAAGGTAGA TCCCAAGAAA
     5341 AAAAGAAAAG TCggatccgg agccacgaac ttctctctgt taaagcaagc aggGgacgtg
     5401 gaagaaaacc ccggtcctAT GCCCGCCATG AAGATCGAGT GCCGCATCAC CGGCACCCTG
     5461 AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG
     5521 ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC
     5581 GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC
     5641 CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC
     5701 GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC
     5761 AAGGTGGTGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC
     5821 AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGTGCTGGT GGGCAGCTTC
     5881 GCCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT TCGTGGTGGA CAGCCACATG
     5941 CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC
     6001 CGCCGCGTGG agGAgCTGCA CAGCAACACC GAGCTGGGCA TCGTGGagTA CCAGCACGCC
     6061 TTCAAGACCC CCATCGCCTT CGCCAGATCT CGAGCTcgaT gagtttggac aaaccacaac
     6121 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6181 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     6241 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtac
     6301 ttaagagggg gagaccaaag ggcgagacgt taaggcctca cgtgacatgt gagcaaaagg
     6361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6541 cctgccgctt acGggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6841 tagaagAaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6901 tggtagctct tgatccggca aacaaaccaC cgctggtagc ggtggttttt ttgtttgcaa
     6961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc cgtctcagaa
     7081 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     7141 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     7201 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     7261 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     7321 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc
     7381 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     7441 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     7501 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     7561 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     7621 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     7681 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     7741 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     7801 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     7861 ttcaatcat
//

