LOCUS       Exported               10118 bp ds-DNA     circular SYN 21-JUL-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUB_SM_ActB_MS2_original
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 26, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     >flag-Halo-baCDS Ligation of Fragment 1 into Fragment 2
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2058..2175
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UBC_human
                     /note="UBC_human promoter"
     CDS             2628..4763
                     /codon_start=1
                     /label=SpaghettiMonster_actb
                     /note="SpaghettiMonster_actb"
                     /translation="MDYKDDDDKGDYKDDDDKGDYKDDDDKGGNSLIKENMRMKVVMEG
                     SVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFGGGSRTFIKYPKGI
                     PDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQK
                     KTKGWEPNTEMMYPADGGLRGYTHMALKVDGGDYKDDDDKQQDYKDDDDKGQQGDYKDD
                     DDKQQDYKDDDDKGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFV
                     VQREHAVAKFAGLGGGGGDYKDDDDKGDYKDDDDKGDYKDDDDKGGGGSGGGGSLQDDD
                     IAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG
                     ILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI
                     MFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLA
                     GRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY
                     ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVL
                     SGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS
                     KQEYDESGPSIVHRKCF"
     CDS             2628..3602
                     /codon_start=1
                     /label=mRuby2_FLAG
                     /note="mRuby2_FLAG"
                     /translation="MDYKDDDDKGDYKDDDDKGDYKDDDDKGGNSLIKENMRMKVVMEG
                     SVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFGGGSRTFIKYPKGI
                     PDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQK
                     KTKGWEPNTEMMYPADGGLRGYTHMALKVDGGDYKDDDDKQQDYKDDDDKGQQGDYKDD
                     DDKQQDYKDDDDKGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFV
                     VQREHAVAKFAGLGGGGGDYKDDDDKGDYKDDDDKGDYKDDDDK"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             2685..2708
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     misc_feature    2898..2906
                     /label=dark chromophore: to make it bright, turn GGG into
                     /note="dark chromophore: to make it bright, turn GGG into"
                     /note="dark chromophore: to make it bright, turn GGG into 
                     MYG."
     CDS             3213..3236
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             3243..3266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             3279..3302
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             3309..3332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             3525..3548
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             3552..3575
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             3639..4760
                     /codon_start=1
                     /label=actb
                     /note="Beta-actin"
                     /translation="DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG
                     MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
                     TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVP
                     IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF
                     EQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSI
                     MKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI
                     GGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
     protein_bind    4774..4803
                     /label=Zipcode
                     /note="Zipcode"
     protein_bind    5230..5252
                     /label=MS2_1
                     /note="MS2_1"
     protein_bind    5283..5305
                     /label=MS2_2
                     /note="MS2_2"
     protein_bind    5336..5358
                     /label=MS2_3
                     /note="MS2_3"
     protein_bind    5390..5410
                     /label=MS2_4
                     /note="MS2_4"
     protein_bind    5442..5464
                     /label=MS2_5
                     /note="MS2_5"
     protein_bind    5495..5517
                     /label=MS2_6
                     /note="MS2_6"
     protein_bind    5548..5570
                     /label=MS2_7
                     /note="MS2_7"
     protein_bind    5601..5623
                     /label=MS2_8
                     /note="MS2_8"
     protein_bind    5654..5676
                     /label=MS2_9
                     /note="MS2_9"
     protein_bind    5707..5729
                     /label=MS2_10
                     /note="MS2_10"
     protein_bind    5760..5782
                     /label=MS2_11
                     /note="MS2_11"
     protein_bind    5813..5835
                     /label=MS2_12
                     /note="MS2_12"
     protein_bind    5866..5888
                     /label=MS2_13
                     /note="MS2_13"
     protein_bind    5919..5941
                     /label=MS2_14
                     /note="MS2_14"
     protein_bind    5972..5994
                     /label=MS2_15
                     /note="MS2_15"
     protein_bind    6025..6047
                     /label=MS2_16
                     /note="MS2_16"
     protein_bind    6078..6100
                     /label=MS2_17
                     /note="MS2_17"
     protein_bind    6131..6153
                     /label=MS2_18
                     /note="MS2_18"
     protein_bind    6184..6206
                     /label=MS2_19
                     /note="MS2_19"
     protein_bind    6237..6259
                     /label=MS2_20
                     /note="MS2_20"
     protein_bind    6290..6312
                     /label=MS2_21
                     /note="MS2_21"
     protein_bind    6343..6365
                     /label=MS2_22
                     /note="MS2_22"
     protein_bind    6396..6418
                     /label=MS2_23
                     /note="MS2_23"
     protein_bind    6449..6471
                     /label=MS2_24
                     /note="MS2_24"
     misc_feature    6508..7096
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6979..6990)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     LTR             7171..7404
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        7609..7713
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             7714..8574
                     /label=AmpR
                     /note="AmpR"
     rep_origin      8745..9333
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9744..9765
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9780..9810
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9818..9834
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9842..9858
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gactacaagg
     2641 acgacgacga caaaggtgac tacaaagatg atgacgataa aggcgactat aaggacgatg
     2701 acgacaaggg cggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
     2761 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
     2821 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
     2881 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
     2941 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
     3001 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
     3061 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
     3121 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctgaggggct
     3181 acacccacat ggcgctgaag gtcgacggag gagattacaa ggatgacgac gataagcaac
     3241 aagattacaa agacgatgat gacaagggcc agcagggcga ctacaaggac gacgacgaca
     3301 agcagcagga ctacaaagat gacgatgata aaggaggagg acatctgtcc tgttcgttcg
     3361 tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa gatgccgggc attcatgccg
     3421 tcgaccaccg cctggagcgg ctcgaagaat cagacaatga gatgttcgtc gtgcaaagag
     3481 aacatgccgt ggccaagttc gcgggactgg gaggcggtgg aggcgattac aaagacgatg
     3541 atgacaaggg tgactataaa gacgacgatg acaaagggga ttacaaggat gatgatgata
     3601 agggaggcgg tggatcaggt ggaggaggtt cactgcagga tgatgatatc gccgcgctcg
     3661 tcgtcgacaa cggctccggc atgtgcaagg ccggcttcgc gggcgacgat gccccccggg
     3721 ccgtcttccc ctccatcgtg gggcgcccca ggcaccaggg cgtgatggtg ggcatgggtc
     3781 agaaggattc ctatgtgggc gacgaggccc agagcaagag aggcatcctc accctgaagt
     3841 accccatcga gcacggcatc gtcaccaact gggacgacat ggagaaaatc tggcaccaca
     3901 ccttctacaa tgagctgcgt gtggctcccg aggagcaccc cgtgctgctg accgaggccc
     3961 ccctgaaccc caaggccaac cgcgagaaga tgacccagat catgtttgag accttcaaca
     4021 ccccagccat gtacgttgct atccaggctg tgctatccct gtacgcctct ggccgtacca
     4081 ctggcatcgt gatggactcc ggtgacgggg tcacccacac tgtgcccatc tacgaggggt
     4141 atgccctccc ccatgccatc ctgcgtctgg acctggctgg ccgggacctg actgactacc
     4201 tcatgaagat cctcaccgag cgcggctaca gcttcaccac cacggccgag cgggaaatcg
     4261 tgcgtgacat taaggagaag ctgtgctacg tcgccctgga cttcgagcaa gagatggcca
     4321 cggctgcttc cagctcctcc ctggagaaga gctacgagct gcctgacggc caggtcatca
     4381 ccattggcaa tgagcggttc cgctgccctg aggcactctt ccagccttcc ttcctgggca
     4441 tggagtcctg tggcatccac gaaactacct tcaactccat catgaagtgt gacgtggaca
     4501 tccgcaaaga cctgtacgcc aacacagtgc tgtctggcgg caccaccatg taccctggca
     4561 ttgccgacag gatgcagaag gagatcactg ccctggcacc cagcacaatg aagatcaaga
     4621 tcattgctcc tcctgagcgc aagtactccg tgtggatcgg cggctccatc ctggcctcgc
     4681 tgtccacctt ccagcagatg tggatcagca agcaggagta tgacgagtcc ggcccctcca
     4741 tcgtccaccg caaatgcttc taggctagct aggcggactg ttactgagct gcgttttaca
     4801 ccctttcttt gacaaaacct aacttgcgca gaaaaaaaaa aaataagaga caacattggc
     4861 atggctttgt ttttttaaat tttttttaaa gttttttttt tttttttttt tttttttttt
     4921 aagttttttt gttttgtttt ggcgcttttg actcaggatt taaaaactgg aacggtgaag
     4981 gcgacagcag ttggttggag caaacatccc ccaaagttct acaaatgtgg ctgaggactt
     5041 tgtacattgt tttgtttttt tttttttttg gttttgtctt tttttaatag tcattccaag
     5101 tatccatgaa ataagtggtt acaggaagtc cctcaccctc ccaaaagcca cccccactcc
     5161 taagaggagg atggtcgcgt ccatgccctg agtccacccc ggggaaggtg acaaccggta
     5221 acctacaaac gggtggagga tcaccccacc cgacacttca caatcaaggg gtacaataca
     5281 caagggtgga ggaacacccc accctccaga cacattacac agaaatccaa tcaaacagaa
     5341 gcaccatcag ggcttctgct accaaattta tctcaaaaaa ctacaacaag gaatcaccat
     5401 cagggattcc ctgtgcaata tacgtcaaac gagggccacg acgggaggac gatcacgcct
     5461 cccgaatatc ggcatgtctg gctttcgaat tcagtgcgtg gagcatcagc ccacgcagcc
     5521 aatcagagtc gaatacaagt cgactttcgc gaagagcatc agccttcgcg ccattcttac
     5581 acaaaccaca ctctccccta caggaacagc atcagcgttc ctgcccagta cccaactcaa
     5641 gaaaatttat gtccccatgc agcatcagcg catgggcccc aagaatacat ccccaacaaa
     5701 atcacatccg agcaccaaca gggctcggag tgttgtttct tgtccaactg gacaaaccct
     5761 ccatggacca tcaggccatg gactctcacc aacaagacaa aaactactct tctcgaagca
     5821 gcatcagcgc ttcgaaacac tcgagcatac attgtgccta tttcttgggt ggacgatcac
     5881 gccacccatg ctctcacgaa tttcaaaaca cggacaagga cgagcaccac cagggctcgt
     5941 cgttccacgt ccaatacgat tacttacctt tcgggatcac gatcacggat cccgcagcta
     6001 catcacttcc actcaggaca ttcaagcatg cacgatcacg gcatgctcca caagtctcaa
     6061 ccacagaaac taccaaatgg gttcagcacc agcgaaccca ctcctacctc aaacctcttc
     6121 ccacaaaact ggcaagcagg atcaccgctt gcccattcca acataccaaa tcaaaaacaa
     6181 ttactggtac agcatcagcg taccagccca catctctcac tactatcaaa aaccaaaccg
     6241 ttcagcaaca gcgaacggta cacacggaaa aatcaactgg tttacaaata cgaaagacga
     6301 tcacgctttc gtccagcgca aactattacg aaaaacatcc gacgggaaga gcaacagcct
     6361 tcccgcggcg gaaaacctca caaaaacacg acaaacggat gcacgaacac ggcatccgcc
     6421 gacaacccac aaacttacaa ccaggcaaac ggtgcaggat caccgcaccg tacatcaaac
     6481 acctcagatc tcatatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     6541 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6601 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6661 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6721 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6781 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6841 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6901 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6961 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7021 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7081 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     7141 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7201 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7381 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7441 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     7501 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7561 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7621 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7681 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7741 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7801 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7861 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7921 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     7981 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8041 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8101 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8161 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8221 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8281 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8341 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8401 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8461 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8521 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8581 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8641 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8701 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8761 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8821 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8881 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8941 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9001 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9061 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9121 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9181 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9241 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9301 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9361 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9421 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9481 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9541 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
     9601 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9661 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
     9721 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9781 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9841 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
     9901 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     9961 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10021 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10081 aattgtattt gttaaatatg tactacaaac ttagtagt
//
