LOCUS       Exported                4866 bp ds-DNA     circular SYN 01-AUG-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pEZ01
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 4, 2016 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     >pAQ1_YFP
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    545..1450
                     /note="Bla AmpR"
     misc_feature    1010..1031
                     /note="back tetO Fwd"
     misc_feature    complement(1010..1031)
                     /note="back tetO Rev"
     misc_feature    1630..2249
                     /note="pBR322 ori"
     misc_feature    2461..2480
                     /note="Primer76"
     misc_feature    2687..3225
                     /note="acsA up"
     misc_feature    2687..2707
                     /note="acsA-UpF(v2)"
     misc_feature    2687..2695
                     /note="acsA-upF(v1)"
     misc_feature    3131..3150
                     /note="Primer ALM 70"
     misc_feature    3203..3225
                     /note="acsA-UpR [EcoRI]"
     misc_feature    3226..3325
                     /note="Promoter"
     misc_feature    3226..3255
                     /note="TetO2* Vec Fwd"
     -10_signal      3297..3302
                     /note="-10"
     misc_feature    3310
                     /note="transcription start site"
     misc_feature    3350..3355
                     /note="RBS"
     misc_feature    3364..4041
                     /note="Thermostable GFP"
     misc_feature    4095..4113
                     /note="T7 terminator"
     misc_feature    4138..4159
                     /note="pAQ1 into pACSA fix2"
     misc_feature    4146..4159
                     /note="Missing Terminator piece"
     misc_feature    4160..4653
                     /note="acsA down"
     misc_feature    4160..4183
                     /note="acsA-DownF [BamHI]"
     misc_feature    4632..4653
                     /note="acsA-DownR(v2)"
     misc_feature    4636..4653
                     /note="acsA-DownR(v1)"
     misc_feature    4849..4866
                     /note="Primer77"
ORIGIN
        1 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
       61 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
      421 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      481 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2641 attacgccaa gctatttagg tgacactata gaatactcaa gctatgaggt gactgccgca
     2701 ctcaatgttc aaccagatct agtgattatt tcggggttag cagccgatgc gggcaacctg
     2761 gtgaagcaac tgcgagaatt aggttacaac ggcattattg ttggggggaa cggcttaaat
     2821 acttctaata ttttccccgt ctgccaagca aaatgtgatg gggtgttggt ggcccaagcc
     2881 tacagtgccg agttagataa tgagattaac cgcgcgtttc gggacgccta ttttcaacaa
     2941 aaccaaaaag agccgcccca atttagtgcc caggctttta cggcgatcca agtttttgtt
     3001 gaagccctca gcagcctcga tgaaaaaacg cccttagaaa ctcttgctct accggacttg
     3061 cgacgacaac tgcgggacga aatttttgca ggtacctacg tcacgccttt gggtgaaatt
     3121 tccttcacag aggaagggga aattgtccag aaggaatttt ttgtggccca aattgaaatg
     3181 gatgaatcgg gtcaacaggg gcgtttcgcc ttcattgaaa cgaacaaagg aattcttaac
     3241 aaaaaagcag gaataaaatt aacaagatgt aacagacata agtcccatca ccgttgtata
     3301 aagttaactg tgggattgca aaaggagctc ccaactcata aagtcaagta ggagattaat
     3361 catatgtcgg tcatcaagcc cgaaatgaaa attaaactgc gcatggaggg cgctgtgaac
     3421 gggcacaagt tcgtgatcga aggtgaaggc attggtaagc cgtatgaagg tactcaaacc
     3481 ctggacttaa cggttaaaga aggtgccccg ttgccattta gctacgacat cctgacaccc
     3541 gcttttcagt atggcaatcg cgcgttcacc aagtatccga aagatattcc ggattatttc
     3601 aagcaggcat tcccggaagg atactcatgg gaacgctcga tgacctacga agaccagggc
     3661 atttgcattg ccacaagcga catcacaatg gagggtgatt gcttttttta caaaattcgc
     3721 ttcgacggga caaacttccc gccgaatggc ccagtgatgc agaaaaagac gcttaaatgg
     3781 gaaccgtcga cagagaaaat gtacgttcgc gatggcgttc tgaaaggcga tgttaatatg
     3841 gcgctgcttc tcgaaggggg aggccattac cgttgtgatt tcaaaaccac atacaaagca
     3901 aaaaaagatg ttcgtttgcc ggacgcgcat gaagtcgatc accgcattga gatcctgtca
     3961 catgataaag attacaataa ggtccgcctt tatgagcacg ctgaggcccg ctattctatg
     4021 ctgcccagcc aagctaaata aggatccggc tgctaacaaa gcccgaaagg aagctgagtt
     4081 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     4141 gacgggtttt ttgtctagag cgctcaagtt gatcgaatgt atgccccgga tatgacctag
     4201 ggagattctt ctaaagcgag cagggtttgc caagtggcga tcgcctcagg gaaccataac
     4261 caactttgtt cgaggcggcg ggggttgagc tgggaacgat cgagatctag ggcgatcgcc
     4321 cggagttttt cggctttccc tacaagaatt tcctggtcaa cgtcggtggg ttgcctttgg
     4381 gccaaatcgt acatactaat cgccaggccc gcataaatct ccgcctggag ggctgggggg
     4441 aaatcctggg tcgttgccgc cgctaaggct tcataccaac gatcattggc ggcttcgtaa
     4501 tctgcattga ggtaatggac aaaacccagg gccatcaaaa tttcggggtc gttgggctgt
     4561 tgggctaggg ccatgtccca attgtcttgc accgtggcga tcgccgttgc ttgggttggc
     4621 gatggtggga cggcagccaa agttgtttgc cagcatgcgc cctatagtga gtcgtattac
     4681 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4741 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4801 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     4861 cattaa
//
